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98 results for “Data Cloning”
Lipid data for clone C43.pMS148.pABW3
<p>Lipid sequence for clone C43.pMS148.pABW3 which produces archaeatidylglycerol. pRSF indicates a clone with an empty plasmid (which results in the control strain). 0.1 MPa or 50 MPa refers to the pressure during shock. 37C or 47C refers to the temperature (in celsius) during shock.</p>
Data from: Can clone size serve as a proxy for clone age? An exploration using microsatellite divergence in Populus tremuloides
In long-lived clonal plant species, the overall size of a clone has previously been used to estimate clone age. The size of a clone, however, might be largely determined by physical or biotic interactions, obscuring the relationship between clone size and age. Here, we use the accumulation of mutations at 14 microsatellite loci to estimate clone age in trembling aspen, Populus tremuloides, from southwestern Canada. We show that the observed patterns of genetic divergence are consistent with a model of clonal growth, allowing us to use pairwise genetic divergence as an estimator of clone age. In the populations studied, clone size did not exhibit a significant relationship with microsatellite divergence, indicating that clone size is not a good proxy for clone age.
Data accompanying "Coherent optical communications using coherence-cloned Kerr soliton microcombs"
<p>This dataset contains measurement data and digital signal processing (DSP) code for the results presented in "Coherent optical communications using coherence-cloned Kerr soliton microcombs". </p> <p> The program code included in this dataset is distributed under a GPLv3 license.</p>
Data from: Genotypic diversity and spatial-temporal distribution of Symbiodinium clones in an abundant reef coral
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Data from: Evaluating the performance of targeted sequence capture, RNA-Seq, and degenerate-primer PCR cloning for sequencing the largest mammalian multigene family
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Data from: Fire coral clones demonstrate phenotypic plasticity among reef habitats
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Data from: Attack of the PCR clones: rates of clonality have little effect on RAD-seq genotype calls
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Data from: Clone configuration and spatial genetic structure of two Halophila ovalis populations with contrasting internode lengths
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Data from: Heritable gene expression differences between apomictic clone members in Taraxacum officinale: insights into early stages of evolutionary divergence in asexual plants
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Data from: Rapid and robust phylotyping of spa t003, a dominant MRSA clone in Luxembourg and other European countries
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Data from: Widespread generalist clones are associated with range and niche expansion in allopolyploids of Pacific Northwest Hawthorns (Crataegus L.)
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Data from: Can clone size serve as a proxy for clone age? An exploration using microsatellite divergence in Populus tremuloides
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Data from: Maladaptation to acute metal exposure in resurrected Daphnia ambigua clones after decades of increasing contamination
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Data from: A multi-genome analysis approach enables tracking of the invasion of a single Russian wheat aphid (Diuraphis noxia) clone throughout the New World
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Data from: Intraspecific variation among clones of a naïve rare grass affects competition with an invasive forb
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Data from: Clones or clans: the genetic structure of a deep-sea sponge, Aphrocallistes vastus, in unique sponge reefs of British Columbia, Canada
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Data from: Positional cloning of rp2 QTL associates the P450 genes CYP6Z1, CYP6Z3 and CYP6M7 with pyrethroid resistance in the malaria vector Anopheles funestus
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Data from: Neutral processes forming large clones during colonization of new areas
In species reproducing both sexually and asexually clones are often more common in recently established populations. Earlier studies have suggested that this pattern arises due to natural selection favouring generally or locally successful genotypes in new environments. Alternatively, as we show here, this pattern may result from neutral processes during species' range expansions. We model a dioecious species expanding into a new area in which all individuals are capable of both sexual and asexual reproduction, and all individuals have equal survival rates and dispersal distances. Even under conditions that favour sexual recruitment in the long run, colonisation starts with an asexual wave. After colonisation is completed, a sexual wave erodes clonal dominance. If individuals reproduce more than one season, and with only local dispersal, a few large clones typically dominate for thousands of reproductive seasons. Adding occasional long-distance dispersal, more dominant clones emerge, but they persist for a shorter period of time. The general mechanism involved is simple: edge effects at the expansion front favour asexual (uniparental) recruitment where potential mates are rare. Specifically, our model shows that neutral processes (with respect to genotype fitness) during the population expansion, such as random dispersal and demographic stochasticity, produce genotype patterns that differ from the patterns arising in a selection model. The comparison with empirical data from a postglacially established seaweed species (Fucus radicans) shows that in this case a neutral mechanism is strongly supported.
Data from: Offspring provisioning explains clone specific maternal age effects on life history and lifespan in the water flea, Daphnia pulex
Genetic inheritance underpins evolutionary theories of aging, but the role that nongenetic inheritance plays is unclear. Parental age reduces the life span of offspring in a diverse array of taxa but has not been explained from an evolutionary perspective. We quantified the effect that maternal age had on the growth and maturation decisions, life history, rates of senescence, and life span of offspring from three Daphnia pulex clones collected from different populations. We then used those data to test general hypotheses proposed to explain maternal age effects on offspring life span. Three generations of breeding from young or old mothers produced dramatic differences in the life histories of fourth-generation offspring, including significant reductions in life span. The magnitude of the effect differed between clones, which suggests that genetic and nongenetic factors ultimately underpin trait inheritance and shape patterns of aging. Older parents did not transmit a senescent state to their offspring. Instead, offspring from older ancestors had increased early-life reproductive effort, which resulted in an earlier onset of reproductive senescence, and an increased rate of actuarial senescence, which shortened their life span. Our results provide a clear example of the need to consider multiple inheritance mechanisms when studying trait evolution.
Data from: PCR duplication: a one-step cloning-free method to generate duplicated chromosomal loci and interference-free expression reporters in yeast
Here, we report on a novel PCR targeting-based strategy called 'PCR duplication' that enables targeted duplications of genomic regions in the yeast genome using a simple PCR-based approach. To demonstrate its application we first duplicated the promoter of the FAR1 gene in yeast and simultaneously inserted a GFP downstream of it. This created a reporter for promoter activity while leaving the FAR1 gene fully intact. In another experiment, we used PCR duplication to increase the dosage of a gene in a discrete manner, from 1× to 2x. Using TUB4, the gene encoding for the yeast γ-tubulin, we validated that this led to corresponding increases in the levels of mRNA and protein. PCR duplication is an easy one-step procedure that can be adapted in different ways to permit rapid, disturbance-free investigation of various genomic regulatory elements without the need for ex vivo cloning.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.