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112 results for “Dugesia”

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dryad32/100

Data from: Outstanding intraindividual genetic diversity in fissiparous planarians (Dugesia, Platyhelminthes) with facultative sex

Background: Predicted genetic consequences of asexuality include high intraindividual genetic diversity (i.e., the Meselson effect) and accumulation of deleterious mutations (i.e., Muller's Ratchet), among others. These consequences have been largely studied in parthenogenetic organisms, but studies on fissiparous species are scarce. Differing from parthenogens, fissiparous organisms inherit part of the soma of the progenitor, including somatic mutations. Thus, in the long term, fissiparous reproduction may also result in genetic mosaicism, besides the presence of the Meselson effect and Muller's Ratchet. Dugesiidae planarians show outstanding regeneration capabilities, allowing them to naturally reproduce by fission, either strictly or combined with sex (facultative). Therefore, they are an ideal model to analyze the genetic footprint of fissiparous reproduction, both when it is alternated with sex and when it is the only mode of reproduction. Results: In the present study, we generate and analyze intraindividual cloned data of a nuclear and a mitochondrial gene of sexual, fissiparous and facultative wild populations of the species Dugesia subtentaculata. We find that most individuals, independently of their reproductive strategy, are mosaics. However, the intraindividual haplotype and nucleotide diversity of fissiparous and facultative individuals is significantly higher than in sexual individuals, with no signs of Muller's Ratchet. Finally, we also find that this high intraindividual genetic diversity of fissiparous and facultative individuals is composed by different combinations of ancestral and derived haplotypes of the species. Conclusions: The intraindividual analyses of genetic diversity point out that fissiparous reproduction leaves a very special genetic footprint in individuals, characterized by mosaicism combined with the Meselson effect (named in the present study as the mosaic Meselson effect). Interestingly, the different intraindividual combinations of ancestral and derivate genetic diversity indicate that haplotypes generated during periods of fissiparous reproduction can be also transmitted to the progeny through sexual events, resulting in offspring showing a wide range of genetic diversity and putatively allowing purifying selection to act at both intraindividual and individual level. Further investigations, using Dugesia planarians as model organisms, would be of great value to delve into this new model of genetic evolution by the combination of fission and sex.

opencc-zeroDec 2018View details →
zenodo32/100

Supplementary material 1 from: Solà E, Sluys R, Segev O, Blaustein L, Riutort M (2015) The taxonomic status of Dugesia biblica from Israel and Turkey (Platyhelminthes, Tricladida, Dugesiidae). ZooKeys 506: 1-12. https://doi.org/10.3897/zookeys.506.9663

Supplementary Table 1: Explanation note: Localities in Israel from which no specimens of Dugesia could be obtained during our samplings.

opencc-by-4.0May 2015View details →
zenodo32/100

Fig. 6 in Dugesia hepta and Dugesia benazzii (Platyhelminthes: Tricladida): two sympatric species with occasional sex?

Fig. 6 Summarization of dataset IV groups and their characteristics. Individuals whose karyotype is stablished are highlighted with bivalent chromosome symbol. Black and white split-up circles indicate individuals with recombinant sequences

opennotspecifiedMay 2020View details →
zenodo32/100

Fig. 2 in Dugesia hepta and Dugesia benazzii (Platyhelminthes: Tricladida): two sympatric species with occasional sex?

Fig. 2 Sampling localities used for this study and geographical distribution of D. hepta and D. benazzii. Localities are numbered according to Supporting information Table S1. Red-colored circles

opennotspecifiedMay 2020View details →
zenodo32/100

Fig. 5 Haplotype networks for datasets IV in Dugesia hepta and Dugesia benazzii (Platyhelminthes: Tricladida): two sympatric species with occasional sex?

Fig. 5 Haplotype networks for datasets IV (a Dunuc12) and III (b Cox1). Haplotypes are depicted as individual circles which are proportional to their abundancy (number of sequences), highlighted in a white square. Mutations are either depicted with black bars or black triangles when the number of mutations between linked haplotypes is equal to or exceeds a certain

opennotspecifiedMay 2020View details →
zenodo32/100

Figure 11 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 11. Dugesia hoidi: A, holotype RMNH.VER.21056.1, photomicrograph showing the penial fold (pf) in sagiưal section; B, paratype RMNH.VER.21056.2, photomicrograph showing the penis papilla (pp) and the penial fold (pf) in transverse section.

opennotspecifiedNov 2023View details →
zenodo32/100

Figure 10. Dugesia hoidi. Holotype RMNH.VER.21056.1 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 10. Dugesia hoidi. Holotype RMNH.VER.21056.1: A, sagiưal reconstruction of the male copulatory apparatus (anterior to the right); B, sagiưal reconstruction of the penial fold and female copulatory apparatus; C, photomicrograph of sagiưal section, showing penis bulb (pb) with the seminal vesicle (sv), right (rvd) and the less (lvd) vas deferens, penis papilla (pp) with the pointed diaphragm (d), and the ejaculatory duct (ed).

opennotspecifiedNov 2023View details →
zenodo32/100

Figure 7. Dugesia benazzii s.s., CGAS Pla 25.1 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 7. Dugesia benazzii s.s., CGAS Pla 25.1: A, sagiưal reconstruction of the male copulatory apparatus (anterior to the right); B, sagiưal reconstruction of the fold and female copulatory apparatus; C, photomicrograph of sagiưal section, showing the penis bulb (pb), penis papilla (pp) with conical, pointed diaphragm (d), ejaculatory duct (ed), penial fold (pf), and 'angled' bursal canal (abc).

opennotspecifiedNov 2023View details →
zenodo32/100

Figure 6 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 6. Karyogram of (A) Dugesia benazzii s.s. from Su Rizzolu River (Oưi, loc. 13) and (B) Dugesia hoidi.

opennotspecifiedNov 2023View details →
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Figure 1. A in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 1. A, sampling localities of the present study. Numbers correspond to population codes listed in Appendix, Table A1 and coincide with those in Dols-Serrate et al. (2020). Red circles indicate populations used for morphological analyses. B, rectangular inset: enlargement of the Bunnari–Mascari confluence area. Ŋe map was created using Q-GIS v.3.2.2 (hưps://qgis.org/es/site/ last accessed September 2023) and edited in ILLUSTÞTOR CC v.22.0.1 (hưps://www.adobe.com/products/illustrator.html last accessed September 2023).

opennotspecifiedNov 2023View details →
zenodo32/100

Figure 14 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 14. Dugesia mariae: A, CGAS Pla 27. 1, photomicrograph showing the penis bulb with the seminal vesicle (sv), less (lvd) and right (rvd) vas deferens, the penial papilla (pp) and the two atrial folds (af) in horizontal section; B, CGAS Pla 27. 4, photomicrograph showing the penis papilla (pp) and the penial fold (pf) in transverse section.

opennotspecifiedNov 2023View details →
zenodo32/100

Figure 9. Dugesia benazzii s.s in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 9. Dugesia benazzii s.s.: A, CGAS Pla 25. 6, photomicrograph showing the penial fold (pf) and the two atrial folds (af) in sagiưal section; B, CGAS Pla 25. 4, photomicrograph showing the penis papilla (pp) and the penial fold (pf) in transverse section.

opennotspecifiedNov 2023View details →
zenodo32/100

Figure 3 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 3. mtDNA (dataset I) phylogenetic tree and species discovery delimitation schemes for ABGD, GMYC, and mPTP, as well as PSHs and PSCs. Ultrametric tree from BEAST is shown only for visual purposes; posterior probabilities (pp) and bootstrap support values (bs) relate to MrBayes and ÞxML analyses, respectively; pp and bs node support values represented by squares and circles, filled with white (unsupported), grey (supported), and black (maximum support), respectively.

opennotspecifiedNov 2023View details →
zenodo32/100

Figure 8. Dugesia benazzii s.s., CGAS Pla 25.1 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 8. Dugesia benazzii s.s., CGAS Pla 25.1. Photomicrograph of the copulatory bursa with a ciliate parasite (cp) and a spermatophore (sp) in a sagiưal section.

opennotspecifiedNov 2023View details →
zenodo32/100

Figure 5 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 5. BFD results for the array of models tested (1–6), with different a priori species-delimitation hypotheses tested on three datasets (I, IV, and V). Each column represents a model with a unique combination of lineages (rows). Star symbol indicates reassignment of PSC4. Marginal-likelihood estimates (MLE) and Bayes' factors comparison results (2lnBf) from the combined analyses of five independent runs with PS (path-sampling) and SS (stepping stone) are represented with circles and a grey-scale scheme. 2lnBf comparison support indicates differences from the best model: non-significant indicates no difference in support for the two models; positively supported and decisively-supported indicate clear support in favour of the best-fiưing model over its alternative.

opennotspecifiedNov 2023View details →
zenodo32/100

Figure 4 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 4. Schematic representation of BPP results on multi-locus data for two topologies. Colour scheme and squares represent posterior probability (pp) for each node under four different prior combinations, using two algorithms (A0 and A1) and three datasets (I, IV, and V). *Unsupported node with a pp of 0.94.

opennotspecifiedNov 2023View details →
zenodo32/100

Figure 2 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 2. Simplified Bayesian (A–E1) and ML dendrograms (E2). A, dataset I: COI–NADH4–tRNAW–COII; B, dataset II: Dunuc10; C, dataset III: Dunuc12; D, dataset IV: Dunuc10 * 12; E, dataset V: mtDNA * nDNA. Posterior probabilities (pp) are indicated by filled squares and bootstrap support values (bs) with filled circles. Full trees are represented in Figure 3 (dataset I) and Supporting Information, Figure S1 (datasets I–V).

opennotspecifiedNov 2023View details →
zenodo32/100

Figure 13. Dugesia mariae. Holotype RMNH.VER.21056.1 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 13. Dugesia mariae. Holotype RMNH.VER.21056.1: A, sagiưal reconstruction of the copulatory apparatus (anterior to the right); B, photomicrograph showing, in a sagiưal section, the penis bulb (pb) with seminal vesicle (sv), penis papilla (pp) with the pointed diaphragm (d), pleated ejaculatory duct (ed), and the penial fold (pf).

opennotspecifiedNov 2023View details →
zenodo32/100

Figure 12 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 12. Dugesia mariae. Photomicrograph of a preserved sexual specimen from the Golo River (Barcheưa, loc. 24).

opennotspecifiedNov 2023View details →
zenodo32/100

Figure 6 in New insights into the evolution and biogeography of freshwater planarians on islands in the Tyrrhenian Sea, Western Mediterranean Basin, with the integrative description of a new endemic species from Corsica (Platyhelminthes: Tricladida: Dugesia)

Figure 6. Dugesia xeropotamica. Photomicrographs of sagittal histological sections. A, paratype GAS Pla 29.1, showing the bursal canal with the ectal reinforcement. B, holotype RMNH.VER.21534.1, showing the extrabulbar penial glands and the penis bulb. Abbreviations: bc, bursal canal; cb, copulatory bursa; cm, circular muscle; e, epithelium; epg, extra bulbar penial glands; er, ectal reinforcement; lm, longitudinal muscle; pb, penis bulb.

opennotspecifiedAug 2024View details →

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