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31 results for “Erysimum”

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zenodo32/100

Fig. 8 in Acropetal and basipetal cardenolide transport in Erysimum cheiranthoides (wormseed wallflower)

Fig. 8. Intermediate cardenolide profiles in inflorescence-grafted plants. Plants were grafted at the inflorescence stage, with the graft junction above the leaves of the stock and below the developing inflorescence of the scion. Cardenolide content was measured in (A) flowers, (B) green siliques, and (C) dry seeds of the grafted plants. The principal component analysis (PCA) is of eight detected cardenolides. Ellipses signify 95% confidence intervals. Bar graphs of the data are in Supplemental Fig. S4.

opennotspecifiedDec 2021View details →
zenodo32/100

Fig. 7 in Acropetal and basipetal cardenolide transport in Erysimum cheiranthoides (wormseed wallflower)

Fig. 7. Cardenolides transport in aboveground tissue. The stalks of three to four-week-old wildtype and 454 cardenolide mutant plants were grafted. Two weeks later, cardenolides were measured in (A) leaves above the graft junction, (B) leaves below the graft junction, (C) stems immediately above the graft junction, and (D) stems immediately below the graft junction. The principal component analysis (PCA) is of eight detected cardenolides. Bar graphs of the data are in Supplemental Fig. S3. Ellipses signify 95% confidence intervals.

opennotspecifiedDec 2021View details →
zenodo32/100

Fig. 5 in Acropetal and basipetal cardenolide transport in Erysimum cheiranthoides (wormseed wallflower)

Fig. 5. Cotyledons retain the maternal plant cardenolide phenotype until true leaf emergence. Cardenolides were measured in F2 progeny of wt x 454 F1 plants. (A) Cardenolide content of single F2 seeds, (B) cardenolide content of F2 cotyledons from individual plants before true leaf emergence, (C) principal component analysis (PCA) of cardenolide content of true leaves of F2 plants, and (D) cardenolide content of F2 true leaves. Different letters indicate P <0.05 differences for each cardenolide, ANOVA followed by Tukey's HSD test. Bars are mean ± s.d. of N = 4–6 homozygotes and 20–24 F2s. wt = wildtype E. cheiranthoides var. Elbtalaue, 454 = 454 cardenolide mutant line. Ellipses in the PCA plot represent 95% confidence intervals. Peak areas were normalized to an ouabain internal standard.

opennotspecifiedDec 2021View details →
zenodo32/100

Fig. 6 in Acropetal and basipetal cardenolide transport in Erysimum cheiranthoides (wormseed wallflower)

Fig. 6. Grafting experiments show that shoot genotype determines root cardenolide phenotype. Seedlings were grafted at the cotyledon stage and cardenolides were measured in leaves and roots after three weeks. (A) Scion (leaf) cardenolides in grafted plants. (B) Stock (root) cardenolides in grafted plants. Different letters indicate P <0.05 differences for each cardenolide, ANOVA followed by Tukey's HSD test. Bars are mean ±s.d. of N =5–13 for shoot samples and 4–10 for root samples. wt = wildtype E. cheiranthoides var Elbtalaue; 454 = 454 cardenolide mutant line. Peak areas were normalized to an ouabain internal standard.

opennotspecifiedDec 2021View details →
zenodo32/100

Fig. 3 in Acropetal and basipetal cardenolide transport in Erysimum cheiranthoides (wormseed wallflower)

Fig. 3. Maternal genotype determines seed cardenolide phenotype. Seed cardenolide content was measured in seeds from naturally self-pollinated and manually crossed plants. Different letters indicate P <0.05 differences for each cardenolide, ANOVA followed by Tukey's HSD test. Bars are mean ± s.d. of N = 4–5. wt = wildtype E. cheiranthoides var. Elbtalaue, 454 = 454 cardenolide mutant line. Peak areas were normalized to an ouabain internal standard.

opennotspecifiedDec 2021View details →
zenodo32/100

Fig. 4 in Acropetal and basipetal cardenolide transport in Erysimum cheiranthoides (wormseed wallflower)

Fig. 4. Cotyledons retain the maternal plant cardenolide phenotype until true leaf emergence. (A) Cardenolide content of cotyledons before true leaf emergence, (B) cardenolide content of cotyledons after true leaf emergence, (C) cardenolide content of true leaves. Different letters indicate P <0.05 differences for each cardenolide, ANOVA followed by Tukey's HSD test. Bars are mean ± s.d. of N = 6–10. wt = wildtype E. cheiranthoides var. Elbtalaue, 454 = 454 cardenolide mutant line. Peak areas were normalized to an ouabain internal standard.

opennotspecifiedDec 2021View details →
zenodo32/100

Fig. 1 in Acropetal and basipetal cardenolide transport in Erysimum cheiranthoides (wormseed wallflower)

Fig. 1. As described by Züst et al. (2020), abundant cardenolides in E. cheiranthoides have digitoxigenin, cannogenol, cannogenin, or strophanthidin as the steroid core. Sugar side chains added to these steroid cores provide additional structural variation. The side chains of Dig-10, Dig-19, Dig-20, and Can-32 have not been fully characterized but are predicted based on MS fragmentation.

opennotspecifiedDec 2021View details →
zenodo32/100

Fig. 2 in Acropetal and basipetal cardenolide transport in Erysimum cheiranthoides (wormseed wallflower)

Fig. 2. Principal component analysis (PCA) biplot of genin abundance as a percentage of total cardenolide abundance. Variable loadings for the first two principal components are displayed as vectors.

opennotspecifiedDec 2021View details →
dryad32/100

Data from: Independent evolution of ancestral and novel defenses in a genus of toxic plants (Erysimum, Brassicaceae)

Open the record for dataset details and reuse information.

publicApr 2020View details →
zenodo28/100

FIGURE 5 in A new perennial Erysimum species from Turkey, E. nemrutdaghense (Brassicaceae)

FIGURE 5. Mitotic metaphase chromosome of Erysimum nemrutdaghense (B.Mutlu 11232), 2n=16.

opennotspecifiedFeb 2018View details →
zenodo20/100

FIGURE 1 in Erysimum hezarense, a new species and Rhammatophyllum gaudanense, a new record of Brassicaceae from Iran

FIGURE 1. Erysimum hezarense sp. nova. A: habit, B: flower, C: mature fruit and orientation of pedicel, D: distribution map of Erysimum hezarense (○ colored in red), E. laxiflorum (◊), E. subulatum (♦), E. iraqense (G) and Rhammatophyllum gaudanense ().

opennotspecifiedAug 2014View details →

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