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33
datasets available to search
ShareScore release 0.9.0
Dataset results
33 results for “Extremophiles”
Molecular Mechanisms Contributing to the Growth and Physiology of an Extremophile Cultured with Dielectric Heating
GEO Series GSE79201. Thermus scotoductus SA-01. 17 samples. Type: Expression profiling by high throughput sequencing.
Abundant and diverse non-coding small RNAs identified in an extremophilic microbial community using metatranscriptomics
GEO Series GSE137164. halite metagenome. 45 samples. Type: Expression profiling by high throughput sequencing.
Purification of micrococcal nuclease (MNase) for use in ribosome profiling of high-salinity extremophiles
GEO Series GSE269836. Haloferax volcanii. 6 samples. Type: Other.
Selection for transcript diversity in the extremophile Galdieria sulphuraria results in retention of the splicing machinery
GEO Series GSE89169. Galdieria sulphuraria. 9 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome analysis of the extremophile tardigrade Hypsibius exemplaris exposed to the DNA-damaging agent bleomycin
GEO Series GSE168917. Hypsibius exemplaris. 24 samples. Type: Expression profiling by high throughput sequencing.
The energy-saving metabolic switch underlies survival of extremophilic red microalgae in extremely high nickel levels
GEO Series GSE284716. Cyanidioschyzon merolae. 29 samples. Type: Expression profiling by high throughput sequencing.
Expression vectors from "Description of a novel extremophile green algae, Chlamydomonas pacifica, and its potential as a biotechnology host."
<h2>Cytosolic vectors for Chlamydomonas reinhardtii</h2> <h3>pAH04 mCherry (5536 bp) <a href="https://www.zotero.org/google-docs/?nUyV9D">(Molino et al., 2018)</a></h3> <ul> <li> <p>AmpR (Ampicillin Resistance):</p> </li> </ul> <p>Positioned at approximately 4000 bp, this gene confers resistance to ampicillin, enabling bacterial selection.</p> <ul> <li> <p>Amp Promoter:</p> </li> </ul> <p>Regulates the expression of the AmpR gene, facilitating its transcription and subsequent resistance to ampicillin.</p> <ul> <li> <p>f1 ori:</p> </li> </ul> <p>Positioned near 5000 bp, this origin of replication (ori) facilitates plasmid replication in bacterial cells, ensuring propagation of the plasmid in E. coli.</p> <ul> <li> <p>PAR1 - HSP70 Promoter + RBCS2 Promoter:</p> </li> </ul> <p>A dual-promoter system that combines the heat-shock protein 70 (HSP70) and ribulose bisphosphate carboxylase small subunit 2 (RBCS2) promoters, providing strong and constitutive expression of downstream genes in Chlamydomonas.</p> <ul> <li> <p>mCherry:</p> </li> </ul> <p>Encodes the mCherry fluorescent protein, a red fluorescent marker commonly used for visualizing gene expression in vivo.</p> <ul> <li> <p>Ble (Bleomycin Resistance):</p> </li> </ul> <p>Provides resistance to bleomycin</p> <ul> <li> <p>Intron and UTRs (rbcS2):</p> </li> </ul> <p>Includes untranslated regions (UTRs) and introns from the rbcS2 gene, enhancing gene expression stability and processing in Chlamydomonas reinhardtii.</p> <ul> <li> <p>XbaI (93 bp) and KpnI (2750 bp):</p> </li> </ul> <p>These are key restriction enzyme sites used for cloning and linearization of the plasmid.</p> <p> </p> <h2>Secretion vectors for <em>Chlamydomonas pacifica</em></h2> <h3>pJPSHx1_PHL7 (5879 bp) <a href="https://www.zotero.org/google-docs/?mCtacP">(Molino, Oliver, et al., 2024)</a></h3> <ul> <li> <p>AmpR (Ampicillin Resistance):</p> </li> </ul> <p>Enables bacterial selection by conferring resistance to ampicillin.</p> <ul> <li> <p>Amp Promoter:</p> </li> </ul> <p>Drives the transcription of the AmpR gene, allowing for the maintenance of plasmids in ampicillin-containing media.</p> <ul> <li> <p>P_TUBA2 Promoter:</p> </li> </ul> <p>Promoter from the tubulin alpha-2 gene from C. pacifica that controls expression of downstream genes, including PHL7, in eukaryotic cells.</p> <ul> <li> <p>T_TUBA2</p> </li> </ul> <p>Terminator region of tubulin alpha-2 gene from C. pacifica</p> <ul> <li> <p>PHL7:</p> </li> </ul> <p>Encodes the PHL7 enzyme, a plastic-degrading enzyme, important for applications in biodegradation and recycling.</p> <ul> <li> <p>F2A Peptide:</p> </li> </ul> <p>A viral peptide sequence that allows for the co-expression and separation of proteins from a single mRNA transcript.</p> <ul> <li> <p>SP7 (Signal Peptide 7):</p> </li> </ul> <p>Directs the PHL7 protein to the secretory pathway for export from the cell.</p> <ul> <li> <p>Step Hygro aph7'' (Hygromycin Resistance):</p> </li> </ul> <p>Provides resistance to hygromycin, facilitating selection of transformed eukaryotic cells.</p> <ul> <li> <p>XbaI (56 bp) and KpnI (3056 bp):</p> </li> </ul> <p>These are key restriction enzyme sites used for cloning and linearization of the plasmid.</p> <p> </p> <p> </p> <p> </p>
Transcriptome sequencing and microarray design for functional genomics in the extremophile Arabidopsis relative Thellungiella salsuginea
GEO Series GSE42156. Arabidopsis thaliana; Eutrema salsugineum. 24 samples. Type: Expression profiling by array.
RNA-seq analysis of the transcriptional response to blue and red light in the extremophilic red alga, Cyanidioschyzon merolae
GEO Series GSE83828. Cyanidioschyzon merolae. 8 samples. Type: Expression profiling by high throughput sequencing.
Identification and Characterization of Cryptic Extremophile Traits by Experimental Evolution
GEO Series GSE76423. Saccharolobus solfataricus. 6 samples. Type: Expression profiling by high throughput sequencing.
RSS mediated persulfidation cascade regulation mechanism of novel transcription factor SscRAc in extremophile Acidithiobacillus caldus under extreme copper stress
GEO Series GSE293048. Acidithiobacillus caldus. 2 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Transcriptional response of the extremophile red alga Cyanidioschyzon merolae to changes in CO2 concentrations
GEO Series GSE100372. Cyanidioschyzon merolae strain 10D. 12 samples. Type: Expression profiling by high throughput sequencing.
RNA-seq analysis of the transcriptional response to blue and red light in the extremophilic red alga, Cyanidioschyzon merolae
GEO Series GSE67303. Cyanidioschyzon merolae strain 10D. 4 samples. Type: Expression profiling by high throughput sequencing.
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