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33 results for “Extremophiles”

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geo24/100

Molecular Mechanisms Contributing to the Growth and Physiology of an Extremophile Cultured with Dielectric Heating

GEO Series GSE79201. Thermus scotoductus SA-01. 17 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenAug 2016View details →
geo24/100

Abundant and diverse non-coding small RNAs identified in an extremophilic microbial community using metatranscriptomics

GEO Series GSE137164. halite metagenome. 45 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenJan 2020View details →
geo24/100

Purification of micrococcal nuclease (MNase) for use in ribosome profiling of high-salinity extremophiles

GEO Series GSE269836. Haloferax volcanii. 6 samples. Type: Other.

openGEO-OpenJun 2024View details →
geo24/100

Selection for transcript diversity in the extremophile Galdieria sulphuraria results in retention of the splicing machinery

GEO Series GSE89169. Galdieria sulphuraria. 9 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenMar 2017View details →
geo24/100

Transcriptome analysis of the extremophile tardigrade Hypsibius exemplaris exposed to the DNA-damaging agent bleomycin

GEO Series GSE168917. Hypsibius exemplaris. 24 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenMay 2024View details →
geo24/100

The energy-saving metabolic switch underlies survival of extremophilic red microalgae in extremely high nickel levels

GEO Series GSE284716. Cyanidioschyzon merolae. 29 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenMay 2025View details →
zenodo24/100

Expression vectors from "Description of a novel extremophile green algae, Chlamydomonas pacifica, and its potential as a biotechnology host."

<h2>Cytosolic vectors for Chlamydomonas reinhardtii</h2> <h3>pAH04 mCherry (5536 bp)&nbsp;<a href="https://www.zotero.org/google-docs/?nUyV9D">(Molino et al., 2018)</a></h3> <ul> <li> <p>AmpR (Ampicillin Resistance):</p> </li> </ul> <p>Positioned at approximately 4000 bp, this gene confers resistance to ampicillin, enabling bacterial selection.</p> <ul> <li> <p>Amp Promoter:</p> </li> </ul> <p>Regulates the expression of the AmpR gene, facilitating its transcription and subsequent resistance to ampicillin.</p> <ul> <li> <p>f1 ori:</p> </li> </ul> <p>Positioned near 5000 bp, this origin of replication (ori) facilitates plasmid&nbsp; replication in bacterial cells, ensuring propagation of the plasmid in E. coli.</p> <ul> <li> <p>PAR1 - HSP70 Promoter + RBCS2 Promoter:</p> </li> </ul> <p>A dual-promoter system that combines the heat-shock protein 70 (HSP70) and ribulose bisphosphate carboxylase small subunit 2 (RBCS2) promoters, providing strong and constitutive expression of downstream genes in Chlamydomonas.</p> <ul> <li> <p>mCherry:</p> </li> </ul> <p>Encodes the mCherry fluorescent protein, a red fluorescent marker commonly used for visualizing gene expression in vivo.</p> <ul> <li> <p>Ble (Bleomycin Resistance):</p> </li> </ul> <p>Provides resistance to bleomycin</p> <ul> <li> <p>Intron and UTRs (rbcS2):</p> </li> </ul> <p>Includes untranslated regions (UTRs) and introns from the rbcS2 gene, enhancing gene expression stability and processing in Chlamydomonas reinhardtii.</p> <ul> <li> <p>XbaI (93 bp) and KpnI (2750 bp):</p> </li> </ul> <p>These are key restriction enzyme sites used for cloning and linearization of the plasmid.</p> <p>&nbsp;</p> <h2>Secretion vectors for <em>Chlamydomonas pacifica</em></h2> <h3>pJPSHx1_PHL7 (5879 bp) <a href="https://www.zotero.org/google-docs/?mCtacP">(Molino, Oliver, et al., 2024)</a></h3> <ul> <li> <p>AmpR (Ampicillin Resistance):</p> </li> </ul> <p>Enables bacterial selection by conferring resistance to ampicillin.</p> <ul> <li> <p>Amp Promoter:</p> </li> </ul> <p>Drives the transcription of the AmpR gene, allowing for the maintenance of plasmids in ampicillin-containing media.</p> <ul> <li> <p>P_TUBA2 Promoter:</p> </li> </ul> <p>Promoter from the tubulin alpha-2 gene from C. pacifica that controls expression of downstream genes, including PHL7, in eukaryotic cells.</p> <ul> <li> <p>T_TUBA2</p> </li> </ul> <p>Terminator region of tubulin alpha-2 gene from C. pacifica</p> <ul> <li> <p>PHL7:</p> </li> </ul> <p>Encodes the PHL7 enzyme, a plastic-degrading enzyme, important for applications in biodegradation and recycling.</p> <ul> <li> <p>F2A Peptide:</p> </li> </ul> <p>A viral peptide sequence that allows for the co-expression and separation of proteins from a single mRNA transcript.</p> <ul> <li> <p>SP7 (Signal Peptide 7):</p> </li> </ul> <p>Directs the PHL7 protein to the secretory pathway for export from the cell.</p> <ul> <li> <p>Step Hygro aph7'' (Hygromycin Resistance):</p> </li> </ul> <p>Provides resistance to hygromycin, facilitating selection of transformed eukaryotic cells.</p> <ul> <li> <p>XbaI (56 bp) and KpnI (3056 bp):</p> </li> </ul> <p>These are key restriction enzyme sites used for cloning and linearization of the plasmid.</p> <p>&nbsp;</p> <p>&nbsp;</p> <p>&nbsp;</p>

opencc-by-4.0Nov 2024View details →
geo24/100

Transcriptome sequencing and microarray design for functional genomics in the extremophile Arabidopsis relative Thellungiella salsuginea

GEO Series GSE42156. Arabidopsis thaliana; Eutrema salsugineum. 24 samples. Type: Expression profiling by array.

openGEO-OpenNov 2013View details →
geo20/100

RNA-seq analysis of the transcriptional response to blue and red light in the extremophilic red alga, Cyanidioschyzon merolae

GEO Series GSE83828. Cyanidioschyzon merolae. 8 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenSep 2016View details →
geo20/100

Identification and Characterization of Cryptic Extremophile Traits by Experimental Evolution

GEO Series GSE76423. Saccharolobus solfataricus. 6 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenDec 2015View details →
geo20/100

RSS mediated persulfidation cascade regulation mechanism of novel transcription factor SscRAc in extremophile Acidithiobacillus caldus under extreme copper stress

GEO Series GSE293048. Acidithiobacillus caldus. 2 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.

openGEO-OpenAug 2025View details →
geo20/100

Transcriptional response of the extremophile red alga Cyanidioschyzon merolae to changes in CO2 concentrations

GEO Series GSE100372. Cyanidioschyzon merolae strain 10D. 12 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenJul 2017View details →
geo20/100

RNA-seq analysis of the transcriptional response to blue and red light in the extremophilic red alga, Cyanidioschyzon merolae

GEO Series GSE67303. Cyanidioschyzon merolae strain 10D. 4 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenSep 2016View details →

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