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81 results for “Fluke”
Fig. 4. A in Prevalence and gross pathology of liver fluke in macropods cohabiting livestock farms in north eastern NSW, Australia, and diagnosis using cELISA
Fig. 4. A. Common wallaroo liver (visceral surface) with prominent fibrotic capsules. B. Liver cross-section of fibrous capsules. C. Eastern grey kangaroo liver (visceral surface) with irregular form, hepatomegaly, fibrotic lesions and bile duct hyperplasia. D. Necrotic tracks generated by immature fluke. E. Immature fluke (mm).
Fig. 3. Rainfall and temperature data throughout 2019–2020 in Prevalence and gross pathology of liver fluke in macropods cohabiting livestock farms in north eastern NSW, Australia, and diagnosis using cELISA
Fig. 3. Rainfall and temperature data throughout 2019–2020 recorded at the Armidale airport NSW, Australia (Australian Government of Bureau of Meteorology, 2019, 2020).
Fig. 1 in Prevalence and gross pathology of liver fluke in macropods cohabiting livestock farms in north eastern NSW, Australia, and diagnosis using cELISA
Fig. 1. Geographical location of livestock farms (A–G) surveyed in the Northern Tablelands region of NSW, Australia, to assess liver fluke prevalence in Macropods (ArcGIS 10.4.1 software, 2018).
Fig. 2 in Prevalence and gross pathology of liver fluke in macropods cohabiting livestock farms in north eastern NSW, Australia, and diagnosis using cELISA
Fig. 2. Liver fluke prevalence in Macropods (infected/total sampled) cohabiting farms in the Northern Tablelands region of NSW, Australia. Number of farms by risk site: low – 2 farms, medium – 3 farms, high – 2 farms.
Fig. 5 in Fish blood flukes (Digenea: Aporocotylidae) from Indonesia: Two new genera and species infecting the banded eagle ray, Aetomylaeus nichofii (Bloch and Schneider, 1801) Capape´and Desoutter, 1979 (Myliobatiformes: Myliobatidae) from Borneo
Fig. 5. Phylogenetic relationships of chondrichthyan blood flukes based on morphological characters (tegumental spines, shape of intestines). Host affiliations are included. Dashed lines indicate species with no nucleotide sequences. Boxes indicate spine rows: blue = 2 + spine rows, green = 1 spine row, and red ⋂ = no spines. Shape of the intestine () inverse U-shaped and (X) X-shaped. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Fig. 1-2. Aetohemecus kirstenjensenae Warren and Bullard n. gen., n in Fish blood flukes (Digenea: Aporocotylidae) from Indonesia: Two new genera and species infecting the banded eagle ray, Aetomylaeus nichofii (Bloch and Schneider, 1801) Capape´and Desoutter, 1979 (Myliobatiformes: Myliobatidae) from Borneo
Fig. 1-2. Aetohemecus kirstenjensenae Warren and Bullard n. gen., n. sp. (Digenea: Aporocotylidae) from the heart of the banded eagle ray, Aetomylaeus nichofii (Bloch and Schneider, 1801) Capape´and Desoutter, 1979 (Myliobatiformes: Myliobatidae). (1) Body of holotype (USNM No. 1642775), dorsal view. Bar = 250 μm. (2) Genitalia, paratype (USNM No. 1642776), ventral view. Bar = 100 μm. Mouth (mo), nerve commissure (nc), oesophagus (os), vitellarium (vit), intestine (i), testis (t), uterus (u), metraterm (met), ovary (o), vas deferens (v), seminal vesicle (sv), cirrus sac (cs), cirrus (c), vitelline duct (vd), common genital pore (cgp), oviducal ampullae (oa), and o¨otype (oo).
Fig. 3-4. Homestios janinecairae Warren and Bullard n. gen., n in Fish blood flukes (Digenea: Aporocotylidae) from Indonesia: Two new genera and species infecting the banded eagle ray, Aetomylaeus nichofii (Bloch and Schneider, 1801) Capape´and Desoutter, 1979 (Myliobatiformes: Myliobatidae) from Borneo
Fig. 3-4. Homestios janinecairae Warren and Bullard n. gen., n. sp. (Digenea: Aporocotylidae) from the heart of the banded eagle ray, Aetomylaeus nichofii (Bloch and Schneider, 1801) Capape´and Desoutter, 1979 (Myliobatiformes: Myliobatidae). (3) Body of holotype (USNM No. 1642774), dorsal view. Bar = 250 μm. (4) Genitalia of holotype (USNM No. 1642774), dorsal view. Bar = 100 μm. Mouth (mo), oesophagus (os), vitellarium (vit), intestine (in), testis (t), ovary (ov), vas deferens (vd), uterus, (u), ascending uterus (au), descending uterus (du), seminal vesicle (sv), cirrus (c), and common genital pore (cgp).
Fig. 5 in Death by massive air sac fluke (Trematoda: Bothriogaster variolaris) infection in a free-ranging snail kite (Rostrhamus sociabilis)
Fig. 5. Bayesian inference phylogenetic tree of the Echinostomatoidea superfamily showing the position of trematodes extracted from Snail Kite from Ecuador (in bold). The tree was built using small subunit of the ribosomal RNA gene (18 S rDNA) in Beast v1.10.4. Bayesian posterior probability values ≥ 0.5 are shown in branches. Family, genus, and species from each sequence are listed along with their accession number. Family delimitations are indicated with grey boxes. Sequences of Schinostomatoidea and Opisthorchioidea were used as outgroups. Scale bar indicates number of expected substitutions per site.
Fig. 4 in Death by massive air sac fluke (Trematoda: Bothriogaster variolaris) infection in a free-ranging snail kite (Rostrhamus sociabilis)
Fig. 4. Trematodes in the right abdominal air sac, serosa of gastrointestinal tract and celomic cavity of a Snail Kite (Rostrhamus sociabilis).
Fig. 2 in Death by massive air sac fluke (Trematoda: Bothriogaster variolaris) infection in a free-ranging snail kite (Rostrhamus sociabilis)
Fig. 2. Trematodes in respiratory system and pneumatic bone of a Snail Kite (Rostrhamus sociabilis): A. Macroscopic image of the lungs, with presence of trematodes (arrows) found during necropsy. B. Trematodes (arrows) colonising the lumen of a tertiary bronchus (10x H&E) C. Trematodes (arrows) in an abdominal air sac (10x H&E) D. A parasite (arrow) in the coracoid bone (10x H&E).
Fig. 1 in Death by massive air sac fluke (Trematoda: Bothriogaster variolaris) infection in a free-ranging snail kite (Rostrhamus sociabilis)
Fig. 1. Trematodes in serosa of heart and proventriculus of Snail Kite (Rostrhamus sociabilis): A. Close up image of a trematode (arrow) in the pericardium during necropsy. B. Histologic capture of a trematode (arrow) present in the serosa of the proventriculus (H&E).
Fig. 3 in Death by massive air sac fluke (Trematoda: Bothriogaster variolaris) infection in a free-ranging snail kite (Rostrhamus sociabilis)
Fig. 3. Trematodes in the liver of a Snail Kite (Rostrhamus sociabilis): A. Trematodes (arrows) in the serosa of proventriculus, gizzard and liver found during postmortem procedure. B. Miracidium (arrow) in the liver parenchyma (40x H&E).
Fig. 6 in Death by massive air sac fluke (Trematoda: Bothriogaster variolaris) infection in a free-ranging snail kite (Rostrhamus sociabilis)
Fig. 6. Full body (A), anterior end (B) and posterior end (C) of trematode Bothrigaster variolaris from a Snail Kite (Rostrhamus sociabilis). T = midbody testes, V = ventral sucker, p = pharynx, O = postesticular ovary, E = eggs.
Linked collectors and determiners for: Description of six new large species of Argentinomyia Lynch-Arribalzaga, 1891 and redescription of Talahua fervida (Fluke, 1945) (Diptera, Syrphidae, Syrphinae).
Natural history specimen data linked to collectors and determiners held within, "Description of six new large species of Argentinomyia Lynch-Arribalzaga, 1891 and redescription of Talahua fervida (Fluke, 1945) (Diptera, Syrphidae, Syrphinae)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="https://bionomia.net/dataset/480966e4-4a9a-4965-91eb-e9b0b4813e15">https://bionomia.net/dataset/480966e4-4a9a-4965-91eb-e9b0b4813e15</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/480966e4-4a9a-4965-91eb-e9b0b4813e15">https://gbif.org/dataset/480966e4-4a9a-4965-91eb-e9b0b4813e15</a>. Formatted as a Frictionless Data package.
Liver fluke (Opisthorchis viverrini) worm burdens and egg counts from humans in Thailand and Lao PDR
<p>Helminth transmission and morbidity are dependent on the number of mature parasites within a host, however, observing adult worms is impossible for many natural infections. An outstanding challenge is therefore relating routine diagnostics, such as faecal egg counts, to the underlying worm burden. This relationship is complicated by density-dependent fecundity (egg output per-worm reduces due to crowding at high burdens) and the skewed distribution of parasites (majority of helminths aggregated in a small fraction of hosts). We address these questions for the carcinogenic liver fluke <em>Opisthorchis viverrini</em>, which infects approximately ten million people across Southeast Asia, by analysing five epidemiological surveys (<em>n </em>= 641) where adult flukes were recovered. Using a mechanistic model, we show that parasite fecundity varies between populations, with surveys from Thailand and Laos demonstrating distinct patterns of egg output and density-dependence. As the probability of observing faecal eggs increases with the number of mature parasites within a host, we quantify diagnostic sensitivity as a function of the worm burden and find that > 50% of cases are misdiagnosed as false negative in communities close to elimination. Finally, we demonstrate that the relationship between observed prevalence from routine diagnostics and true prevalence is non–linear and strongly influenced by parasite aggregation.</p>
Knockout of liver fluke granulin, Ov-grn-1, impedes malignant transformation during chronic infection with Opisthorchis viverrini: BrdU staining and fibrosis
<p>Knockout of liver fluke granulin, Ov-grn-1, impedes malignant transformation during chronic infection with Opisthorchis viverrini:</p> <p>1. BrdU stained images for the detection of proliferation with manual counting of brown stained cell proportion. Each group in a seperate file BRDUxxx</p> <p>2. Fibrosis local images stained with Sirius red with automated analysis with imageJ. Grouped into one zip file</p> <p>3. Global analysis of liver fibrosis pathogenesis (Ishak scores) performed by 2 blinded pathologists. Grouped into one zip file</p> <p>Methods in associated publication. Briefly 3 groups of 15 hamsters were infected with 100 NEJ flukes that had been gene edited with CRISPR/Cas9 plasmids targeting either Ov-grn-1, Ov-tsp-2, or a scrambled sequence as the control group. Images in these files are the micrographs from these three groups of hamsters. Numbered 1-15 representing the hamster and left/middle/right representing the liver lobe the section was taken from.</p> <p> </p> <p>Knockout of liver fluke granulin, Ov-grn-1, impedes malignant transformation during chronic infection with Opisthorchis viverrini</p> <p>ABSTRACT: Infection with the food-borne liver fluke <em>Opisthorchis viverrini</em> is the principal risk factor for cholangiocarcinoma (CCA) in the Mekong Basin countries of Thailand, Lao PDR, Vietnam, Myanmar and Cambodia. Using a novel model of CCA, involving infection with gene-edited liver flukes in the hamster during concurrent exposure to dietary nitrosamine, we explored the role of the fluke granulin-like growth factor <em>Ov</em>-GRN-1 in malignancy. We derived RNA-guided gene knockout flukes (<em>ΔOv-grn-1)</em> using CRISPR/Cas9/gRNA materials delivered by electroporation. Genome sequencing confirmed programmed Cas9-catalyzed mutations of the targeted genes, which was accompanied by rapid depletion of transcripts and the proteins they encode. Gene-edited parasites colonized the biliary tract of hamsters and developed into adult flukes. However, less hepatobiliary tract disease manifested during chronic infection with <em>ΔOv-grn-1</em> worms in comparison to hamsters infected with control gene-edited and mock-edited parasites. Specifically, immuno- and colorimetric-histochemical analysis of livers revealed markedly less periductal fibrosis surrounding the flukes and less fibrosis globally within the hepatobiliary tract during infection with <em>ΔOv-grn-1</em> genotype worms, minimal biliary epithelial cell proliferation, and significantly fewer mutations of <em>TP53</em> in biliary epithelial cells. Moreover, fewer hamsters developed high-grade CCA compared to controls. The clinically relevant, pathophysiological phenotype of the hepatobiliary tract confirmed a role for this secreted growth factor in malignancy and morbidity during opisthorchiasis.</p>
Data from: Parasite spillover rather than niche expansion explains infection of host brain by diplostomid eye flukes
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Liver fluke (Opisthorchis viverrini) worm burdens and egg counts from humans in Thailand and Lao PDR
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Supporting information for "Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni"
<ul> <li>Files “CG_Ro_1_High Sensitivity DNA Assay_DE13805677_2019-06-20_09-10-48.pdf” and “CG_Ro_2_High Sensitivity DNA Assay_DE13805677_2019-06-20_10-12-12.pdf” uncropped files used in figure 2</li> <li>File “gel qpcr test input sds_01.tif” : uncropped file used in figure 8.</li> <li>File "20200612-1_Report.pdf" : qPCR report for inputs 1µL and available chromatine ("Row7"), figure 6 </li> <li>File "20200919-1_Report.pdf" : qPCR report for for testing SDS after Tn5, figure 7B and figure 8</li> <li>File "20200921-1_Report.pdf" : qPCR report for for comparing inputs with enzyme of Diagenode kit and our protocol with other enzyme Tn5 </li> <li>File "wilcoxon_curves.tgz" contains compressed versions of R-script "wilcoxon_curves.Rmd" that was used to compared metagene profiles and generate "wilcoxon_curves.html" and underlying ressources that are also in this compressed archive</li> </ul> <p>Produced at IHPE (http://ihpe.univ-perp.fr)</p>
Genomic data reveal a North-South split and introgression history of blood fluke populations across Africa
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