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Fig. 6 in Unraveling a new lineage of Hydrobiidae genera (Caenogastropoda: Truncatelloidea) from the Ponto-Caspian region

Fig. 6. Shell, operculum and radula of Persipyrgula saboori (Glöer & Pešić, 2009) gen. et comb. nov. from Zou Eram spring in Zou Eram village, Khorrasan Province, Iran. A–B. Shell in front view. C. Shell in lateral view. D. Protoconch and microsculpture. E–F. Internal and external side of the operculum. G. Rows of teeth of the radula. H. Central teeth. I. Detail of outer marginal teeth.

Fig. 1 in Unraveling a new lineage of Hydrobiidae genera (Caenogastropoda: Truncatelloidea) from the Ponto-Caspian region

Fig. 1. Bayesian inference of hydrobiid species based on COI sequences. Values below branches indicate bootstrap supports for maximum likelihood and BPPs for Bayesian inference. Black bars on the right denote subfamily assignments. Scale bar: expected change per site.

Fig. 8 in Unraveling a new lineage of Hydrobiidae genera (Caenogastropoda: Truncatelloidea) from the Ponto-Caspian region

Fig. 8. Shell, operculum and radula of Shadinia terpoghassiani (Shadin, 1952) from Lake Aiger-Lich, Armenia. A–C. Shell in front view. D. Protoconch and microsculpture. E–F. Internal and external side of the operculum. G. Rows of teeth of the radula. H. Central teeth. I. Detail of outer marginal teeth.

Fig. 2 in Unraveling a new lineage of Hydrobiidae genera (Caenogastropoda: Truncatelloidea) from the Ponto-Caspian region

Fig. 2. Shell, operculum and radula of Intermaria zagrosensis (Glöer & Pešić, 2009) gen. et comb. nov. from Sar Pol Kangarar stream, Kermanshah Province, Iran. A–B. Shell in front view. C. Shell in lateral view. D. Protoconch and microsculpture. E–F. Internal and external side of the operculum. G. Rows of teeth of the radula. H. Central teeth. I. Detail of outer marginal teeth.

Fig. 7 in Unraveling a new lineage of Hydrobiidae genera (Caenogastropoda: Truncatelloidea) from the Ponto-Caspian region

Fig. 7. Anatomy of Persipyrgula saboori (Glöer & Pešić, 2009) gen. et comb. nov. from Zou Eram spring in Zou Eram village, Khorrasan Province, Iran. A. Ctenidium and osphradium. B. Partial nervous system. C. Female genitalia. D. Bursa copulatrix and seminal receptacle. E–F. Head of a male and penis. G. Prostate gland. Anatomical abbreviations given in the Material and methods section.

Fig. 4 in Unraveling a new lineage of Hydrobiidae genera (Caenogastropoda: Truncatelloidea) from the Ponto-Caspian region

Fig. 4. Shell, operculum and radula of Intermaria kermanshahensis (Glöer & Pešić, 2009) gen. et comb. nov. from a spring near Sarabe - Sahneh city, Kermanshah Province, Iran. A–B. Shell in front view. C. Shell in lateral view. D. Protoconch and microsculpture. E–F. Internal and external side of the operculum. G. Rows of teeth of the radula. H. Central teeth. I. Detail of outer marginal teeth.

Fig. 5 in Unraveling a new lineage of Hydrobiidae genera (Caenogastropoda: Truncatelloidea) from the Ponto-Caspian region

Fig. 5. Anatomy of Intermaria kermanshahensis (Glöer & Pešić, 2009) gen. et comb. nov. from a spring near Sarabe - Sahneh city, Kermanshah Province, Iran. A. Ctenidium and osphradium. B. Partial nervous system. C. Female genitalia. D. Bursa copulatrix and seminal receptacle. E–F. Head of a male and penis. G. Prostate gland. Anatomical abbreviations given in the Material and methods section, except DL (distal lobe).

Figure 5 in Salenthydrobia gen. nov. (Rissooidea: Hydrobiidae): a potential relict of the Messinian salinity crisis

Figure 5. Unrooted COI ML trees for populations of taxon A (three populations, 15 specimens), Adriohydrobia gagatinella (four populations, 40 specimens; adapted from Wilke & Falniowski, 2001), and Peringia ulvae (three populations, 14 specimens; modified from Wilke & Davis, 2000; see text for details). Circle sizes are proportional to the observed number of individuals with each haplotype. Missing haplotypes are indicated by small black circles. All individuals of taxon A are areacoded (Ba = Bambinello Spring, Id = Idume Creek, TC = Torre Castiglione).

Figure 6 in Salenthydrobia gen. nov. (Rissooidea: Hydrobiidae): a potential relict of the Messinian salinity crisis

Figure 6. Salenthydrobia ferrerii sp. nov., individuals preserved in ethanol. A, holotype (ANSP, A19754). B-D, paratypes (ANSP, A19755). E, F, paratypes (SMF 323031/2).

Figure 3 in Salenthydrobia gen. nov. (Rissooidea: Hydrobiidae): a potential relict of the Messinian salinity crisis

Figure 3. Maximum likelihood tree for hydrobiine taxa based on 1127 bp of the combined COI and 16S genes. Mercuria similis and Pseudamnicola lucensis were used as outgroups. The scale bar indicates the expected number of substitutions according to the model of sequence evolution applied. For taxon A, individual DNA isolation numbers are given. Bootstrap values (in percentage) are provided (for details see text).

Figure 1 in Salenthydrobia gen. nov. (Rissooidea: Hydrobiidae): a potential relict of the Messinian salinity crisis

Figure 1. Southern Italy with sampling localities on the Salentina Peninsula. The shaded areas show the land mass after the Pliocene flooding (about 4.5 Mya).

Figure 2 in Salenthydrobia gen. nov. (Rissooidea: Hydrobiidae): a potential relict of the Messinian salinity crisis

Figure 2. Head-penis complex in the four known genera of the Hydrobiinae (sensu Radoman, 1977): Hydrobia (A), Peringia (B), Ventrosia (C), Adriohydrobia (D), as well as in taxon A (E). Note that the general penis morphology is constant within the putative genera of the Hydrobiinae. All drawings are to scale.

Figure 4 in Salenthydrobia gen. nov. (Rissooidea: Hydrobiidae): a potential relict of the Messinian salinity crisis

Figure 4. Time scale for cladogenic events in hydrobiine taxa based on a ML analysis of the COI gene under the constraint of the molecular clock hypothesis. The outgroup taxa were removed a postori. The thick bars represent population divergence (±SE) for the two nodes involving the splits of taxon A as well as Adriohydrobia. Rates of evolution (1–3% population divergence per Myr) are shown above the time scale.

Fig. 12. Holotypes. A–C. H in On the origin and diversification of the stygobiotic freshwater snail genus Hauffenia (Caenogastropoda: Hydrobiidae) with special focus on the northern species and the description of two new species

Fig. 12. Holotypes. A–C. H. lozekiana sp. nov. (NHMW 113638). D–E. H. steffeki sp. nov. (NHMW 113640). Scale bar = 500 µm.

Fig. 1 in On the origin and diversification of the stygobiotic freshwater snail genus Hauffenia (Caenogastropoda: Hydrobiidae) with special focus on the northern species and the description of two new species

Fig. 1. Current distribution of Hauffenia Pollonera, 1898 in relation to the extension of the Pannonian Sea 10 Ma.

Fig. 17 in On the origin and diversification of the stygobiotic freshwater snail genus Hauffenia (Caenogastropoda: Hydrobiidae) with special focus on the northern species and the description of two new species

Fig. 17. Penes (SEM micrographs). A–B. H. lozekiana sp. nov. (different specimens). C–E. H. steffeki sp. nov. (one specimen). Arrow points at penial stylet, which is withdrawn in A and B. Scale bars: A–C = 50 µm; D = 10 µm; E = 2 µm.

Fig. 9 in On the origin and diversification of the stygobiotic freshwater snail genus Hauffenia (Caenogastropoda: Hydrobiidae) with special focus on the northern species and the description of two new species

Fig. 9. Phylogenetic time-tree from Bayesian analysis in BEAST based on COI. Specimens identified by two-digit locality code in bold, four digit individual DNA code, and orographic unit (see Table 1) or GenBank accession number (from Wilke & Davis 2000; Wilke 2003; Ponder et al. 2008; Falniowski & Szarowska 2015; Barco et al. 2016; Rysiewska et al. 2017); posterior probabilities and highest posterior density intervals at nodes; arrow points at calibrated node; scale bar in substitutions per site; timeline on bottom in Ma before present.

Fig. 6 in On the origin and diversification of the stygobiotic freshwater snail genus Hauffenia (Caenogastropoda: Hydrobiidae) with special focus on the northern species and the description of two new species

Fig. 6. Phylogenetic tree from Bayesian analysis in MrBayes based on 16S rRNA and ITS2 (outgroup pruned off). Specimens identified by two-digit locality code in bold, four digit individual DNA code, and orographic unit (see Table 1) or GenBank accession number (from Ponder et al. 2008); posterior probabilities at nodes; scale bar in substitutions per site.

Fig. 5 in On the origin and diversification of the stygobiotic freshwater snail genus Hauffenia (Caenogastropoda: Hydrobiidae) with special focus on the northern species and the description of two new species

Fig. 5. Type locality of H. lozekiana sp. nov. (71) and Dabarska Pećina, a locality of H. steffeki sp. nov. (72) (see Table 1).

Fig. 14 in On the origin and diversification of the stygobiotic freshwater snail genus Hauffenia (Caenogastropoda: Hydrobiidae) with special focus on the northern species and the description of two new species

Fig. 14. Protoconchs (SEM micrographs). A. H. lozekiana sp. nov., paratype (NHMW 113639). B. H. steffeki sp. nov., paratype (NHMW 113641). Scale bars = 100 µm.