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20,982 results for “Island”
A droplet digital polymerase chain reaction assay to detect rare helminth parasites infecting natural host populations (Vancouver Island 2023, University of Wisconsin Madison Laboratory colony 2024)
Helminth infections represent a significant challenge to human, livestock, and wildlife health, yet they remain relatively under-studied, especially in terms of their ecological impacts. Better understanding of how these parasites spread in wildlife populations could improve our ability to predict and manage disease transmission across various species. Traditional detection methods, such as visually identifying parasites in environmental samples or infected hosts, often fall short, especially during the early stages of infection when parasite loads are minimal. In this study, we introduce a highly sensitive and precise droplet digital PCR (ddPCR) assay that quantifies helminth DNA in aquatic habitats, focusing on the 18S rRNA gene as a marker. These data utilize the model host-parasite system between the tapeworm Schistocephalus solidus, and its cyclopoid copepod host, Acanthocyclops robustus. The molecular assays are built around creating an infection standard in the lab, where copepods were singly infected with a single tapeworm parasite. We extracted DNA from 100 infected adults and used this as a standard to translate gene copy numbers from the ddPCR reactions to actual animal values. After creating a known lab standard, we then use the generated probes and primers to detect (and quantify!) infection burdens in field samples, which include both water filter samples (eDNA) and zooplankton tows from several lakes around Vancouver Island, B.C. The data presented here include well-specific data from ddPCR runs (amplitude of individual level oil droplets in the reaction) as well as each ddPCR analysis in its entirety. In order to prove the specificity of probes and probe-primers, we include here ddPCR runs of closely related helminth species, Schistocephalus cotti and Schistocephalus pungitii. We also consider the binding to another genera of copepod, the calanoid Eurytomora. All of the data wrangling, analysis, and data visualization are included as .Rmd files in th
GRIME AI Water Segmentation Model for the USGS Platte River near Grand Island, NE, 2023-2024
Ground-based observations from fixed-mount cameras have the potential to fill an important role in environmental sensing, including direct measurement of water levels and qualitative observation of ecohydrological research sites. All of this is theoretically possible for anyone who can install a trail camera. Easy acquisition of ground-based imagery has resulted in millions of environmental images stored, some of which are public data, and many of which contain information that has yet to be used for scientific purposes. The goal of this project was to develop and document key image processing and machine learning workflows, primarily related to semi-automated image labeling, to increase the use and value of existing and emerging archives of imagery that is relevant to ecohydrological processes. This data package includes imagery, annotation files, water segmentation model and model performance plots, and model test results (overlay images and masks) for the Platte River, near Grand Island, NE, 2023-2024. All imagery was acquired from the USGS Hydrologic Imagery Visualization and Information System (HIVIS; see https://apps.usgs.gov/hivis/camera/NE_Platte_River_near_Grand_Island for this specific data set) and/or the National Imagery Management System (NIMS) API. Water segmentation models were created by tuning the open-source Segment Anything Model 2 (SAM2, https://github.com/facebookresearch/sam2) using images that were annotated by team members on this project. The models were trained on the "water" annotations, but annotation files may include additional labels, such as "snow", "sky", and "unknown". Image annotation was done in Computer Vision Annotation Tool (CVAT) and exported in COCO format (.json). All model training and testing was completed in GaugeCam Remote Image Manager Educational Artificial Intelligence (GRIME AI, https://gaugecam.org/) software (Version: Beta 16). Model performance plots were automatically generated during this process. This project w
Long-term monitoring of seven high marsh plant mixtures on Sapelo Island GA
We set up plots (1x2m) in 1996 in mixtures or on vegetation borders at three sites. We set up 16 plots for each of seven mixtures - Marsh Landing (Juncus-Meadow, Spartina-Meadow, and Spartina-Batis mixtures), Teal Boardwalk (Juncus-Spartina mixture) and Shell Hammock (Juncus-Spartina, Juncus-Meadow, and Spartina-Meadow mixtures). Half were watered with fresh water 3 times per week (W) and half were controls (C). Watering stopped in April 2000. Plots were monitored to document changes in plant community composition (percent cover) over time.
Long-term soil salinity and organic content from the Georgia Coastal Ecosystems LTER Project on Sapelo Island, Georgia
Soil samples were collected in conjunction with annual plant monitoring at half of the permanent vegetation monitoring plots in the creekbank and midmarsh zones at 10 GCE study sites beginning in 2009. Pore-water salinity was determined by analysis of supernatant salinity in dried soil samples hydrated with a measured volume of deionized water. Organic content was measured gravimetrically by comparing ash-free dry weight and total weight of soil samples.
Wrack classification data based on UAV imagery from Dean Creek on Sapelo Island, GA
We used a DJI Matrice 210 UAV with a MicaSense Altum to collect a total of 20 images from January 2020 - December 2021 in a the Dean Creek marsh on Sapelo Island, GA. Wrack was classified using a principal component analysis. Wrack patches under 1 m2 were excluded from analyses. Wrack classifications were converted to polygon and point data where each point represents a 5 cm x 5 cm pixel. Those files were then used to analyze wrack characteristics, their relation to environmental drivers, and landscape based patterns. For both polygon and point data, we used the National Elevation Dataset (https://gdg.sc.egov.usda.gov/Catalog/ProductDescription/NED.html) to determine the elevation of each wrack patch. Creeks and shorelines were digitized and used to determine each wrack patches' distance to water. We calculated the frequency of wrack deposition at each point by adding together the number of images where that pixel was classified as wrack over the course of the study. Polygon data were related to tide height from a NOAA tidal station data product (Ft. Pulaski, Station 8670870; https://tidesandcurrents.noaa.gov) and wind speed and wind direction from the Marsh Landing weather station (downloaded data for the SAPMLMET met station from: https://cdmo.baruch.sc.edu/) to evaluate the relationship of wrack to environmental drivers.
Comparing vertical accretion, organic carbon (C) sequestration, and nitrogen burial between a natural, never diked tidal salt marsh and a hydrologically restored tidal salt marsh on Sapelo Island, Georgia.
Restoration of tidal marshes throughout the 20th century have attempted to bring back important functions of natural tidal systems. In this study, vertical accretion, organic carbon (C) sequestration, and nitrogen burial were compared between a natural, never diked tidal salt marsh and a hydrologically restored tidal salt marsh on Sapelo Island, Georgia to examine the impacts of restoration years later. On Sapelo Island there are two marshes near the University of Georgia Marine Institute, one of which is a natural marsh, and one of which is a restored marsh. The restored marsh had been diked in 1948, and the dike was breached, allowing for the marsh to be restored, in 1956. Soil cores were collected from both marshes, and the sediments were analysed for Nitrogen and Carbon concentrations and bulk density. This analysis was used to determine accretion rates for the two marshes as well as changes in the restored marsh since the dike was breached. Nitrogen burial, carbon sequestration, and soil accretion in the restored marsh as compared to the natural marsh were the focus of this study.
Elevation and soil salinity transects at Airport Marsh and Old Beach Road on Sapelo Island, Georgia in 2001
To examine the relationship between elevation and soil characteristics, I sampled two sites on Sapelo Island (Old Beach Road and Airport Marsh) in July of 2001. At each site, I collected 160 soil samples across a transect from the high to the low marsh, and surveyed the relative elevation of each sample location with a theodolite. I determined soil water content gravimetrically, soil organic content by ashing samples, and soil salinity by rehydrating dried soils, adding deionized water, measuring the salinity of the supernatant, and back calculating to the original soil water content.
Borrichia removal experiment at Dean Creek marsh on Sapelo Island, Georgia between 1995 and 2001
To examine the effect of Borrichia on Batis and Sarcocornia sp., I completely clipped all vegetation from six 0.5 x 0.5 m quadrats in a mixed stand of the three species at the Dean Creek marsh in June 1995. Six additional plots were left unmanipulated as controls. Removal and control plots were fully interspersed. I clipped Borrichia from the removal plots every 6 months but allowed other plant species to re-colonize.
Marsh vegetation and soil survey at Marsh Landing, Sapelo Island GA from July 1999.
To document edaphic and vegetation patterns in a Georgia marsh, I sampled seven vegetation "zones" at Marsh Landing on Sapelo Island in July, 1999. Vegetation zones were delineated based on vegetation composition. I located 8 transects running from the Juncus zone in the high marsh down into the short S. alterniflora zone. The tall Spartina zone was not sampled. I sampled a single quadrat (0.25 m x 0.25 m) in each vegetation zone along each transect, for a total of 56 plots. I measured canopy height with a meter stick. I harvested aboveground biomass within each quadrat, sorted it to species, dried and weighed it. I measured soil water content gravimetrically by drying surface (4 cm deep) soil cores and expressing results as (water mass)/(mass of wet core). I determined porewater salinity by rehydrating dried soil cores with a known volume of deionized water, measuring the salinity of the supernatant after 48 h, and back-calculating to the volume of water originally present. I measured soil organic content as loss on ignition of dried soil cores at 450oC for 12 h. I measured relative elevation of each vegetation zone with a theodolite, with the elevation of the lowest zone arbitrarily set to zero. The resulting dataset describes how plant species composition, richness, height and biomass varies as a function of abiotic conditions in the upper part of a Georgia marsh.
Lake Sediment Pollen from Wildwood Lake in Long Island NY from 4500 BP to Present
Analyses of pollen, charcoal, and organic content in a lake-sediment core from Wildwood Lake, Long Island, New York, provide insights into the ecological and environmental history of this region. The early-Holocene interval of the record (~9800-8800 cal. a BP) indicates the presence of Pinus rigida-Quercus ilicifolia woodlands with high fire activity. A layer of sandy sediment dating to 9200 cal. a BP may reflect a brief period of reduced water depth, consistent with widespread evidence for cold, dry conditions at that time. Two other sandy layers, bracketed by 14C dates, represent a sedimentary hiatus from ~8800 to 4500 cal. a BP. This discontinuity may reflect the removal of some sediment during brief periods of reduced water depth at 5300 and 4600 cal. a BP. In the upper portion of the record (before 4500 cal. a BP), subtle changes at ~3000 cal. a BP indicate declining prevalence of Quercus-Fagus-Carya forests and increasing abundance of Pinus rigida, perhaps due to reduced summer precipitation. Elevated percentages of herbaceous taxa in the uppermost sediments represent European agricultural activities. However, unlike charcoal records from southern New England, fire activity does not increase dramatically with European settlement. These findings indicate that present-day Pinus rigida-Quercus ilicifolia woodlands on eastern Long Island are not a legacy of recent, anthropogenic disturbances.
Land Cover on the Elizabeth Islands, Martha's Vineyard, and Nantucket 1938
The widespread influence of land use and natural disturbance on population, community, and landscape dynamics and the long-term legacy of disturbance on modern ecosystems requires that a historical, broad-scale perspective become an integral part of modern ecological studies and conservation assessment and planning. In previous studies, the Harvard Forest Long Term Ecological Research (LTER) program has developed an integrated approach of paleoecological and historical reconstruction, meteorological modeling, air photo interpretation, GIS analyses, and field studies of vegetation and soils, to address fundamental ecological questions concerning the rates, direction, and causes of vegetation change, to evaluate controls over modern species and community distributions and landscape patterns, and to provide critical background for conservation and restoration planning. In the current study, we extend this approach to investigate the link between landscape history and the abundance, distribution, and dynamics of species, communities and landscapes of the Cape Cod to Long Island coastal region, including the islands of Martha's Vineyard, Nantucket, and Block Island. The study region includes many areas of high conservation priority that are linked geographically, historically, and ecologically. This dataset includes a land cover GIS layer created from aerial photographs from 1938. Janice Stone interpreted the photos onto acetates which were then redrawn onto USGS topographic maps using a zoom transfer scope to reduce edge distortion from the photographs. The landcover polygons were then digitized into a GIS. As 1938 is near the midpoint between the peak of 19th century agricultural land clearance and the modern plant communities of the region, this data provides valuable information on changing landscape characteristics and vegetation successional patterns which shape the modern landscape.
Lake Sediment Pollen and Charcoal from Blaney's Pond in the Elizabeth Islands MA from 13582 BP to Present
Aim We analyzed a dataset composed of multiple palaeoclimate and lake-sediment pollen and charcoal records from New England to explore how postglacial changes in forest composition and spatial patterns of vegetation and fire were controlled by regional-scale climate change, a subregional environmental gradient, and landscape-scale variations in soil characteristics. Location The 120,000-km2 study area includes parts of Vermont and New Hampshire in the north, where sites are 150-200 km from the Atlantic Ocean, and spans the coastline from southeastern New York to Cape Cod and the adjacent islands, including Block Island, the Elizabeth Islands, Nantucket, and Martha’s Vineyard. Results Boreal forest featuring Picea and Pinus banksiana was present across the region when conditions were cool and dry 14,000-12,000 calibrated 14C yrs before present (ybp). Pinus strobus became regionally dominant as temperatures increased between 12,000 and 10,000 ybp. The composition of forests in inland and coastal areas diverged in response to further warming after 10,000 ybp, when Quercus and Pinus rigida expanded across southern New England, while conditions remained cool enough in inland areas to maintain Pinus strobus. Fire severity was high during 10,000-8000 ybp. Increasing precipitation allowed Tsuga canadensis, Fagus grandifolia, and Betula to replace Pinus strobus in inland areas during 9000-8000 ybp, and also led to the expansion of Carya across the coastal part of the region beginning at 7000-6000 ybp. Abrupt cooling at 5500-5000 ybp caused sharp declines in Tsuga in inland areas and Quercus at some coastal sites, and the populations of those taxa remained low until they recovered around 3000 ybp in response to rising precipitation. Throughout most of the Holocene, sites underlain by sandy glacial deposits were occupied by Pinus rigida and Quercus. Main conclusions Postglacial changes in the composition and spatial pattern of New England forests were controlled by long-term t
Lake Sediment Pollen from Fresh Pond on Block Island RI from 13581 BP to Present
Aim We analyzed a dataset composed of multiple palaeoclimate and lake-sediment pollen records from New England to explore how postglacial changes in the composition and spatial patterns of vegetation were controlled by regional-scale climate change, a subregional environmental gradient, and landscape-scale variations in soil characteristics. Location The 120,000-km2 study area includes parts of Vermont and New Hampshire in the north, where sites are 150-200 km from the Atlantic Ocean, and spans the coastline from southeastern New York to Cape Cod and the adjacent islands, including Block Island, the Elizabeth Islands, Nantucket, and Martha’s Vineyard. Results Boreal forest featuring Picea and Pinus banksiana was present across the region when conditions were cool and dry 14,000-12,000 calibrated 14C yrs before present (ybp). Pinus strobus became regionally dominant as temperatures increased between 12,000 and 10,000 ybp. The composition of forests in inland and coastal areas diverged in response to further warming after 10,000 ybp, when Quercus and Pinus rigida expanded across southern New England, while conditions remained cool enough in inland areas to maintain Pinus strobus. Increasing precipitation allowed Tsuga canadensis, Fagus grandifolia, and Betula to replace Pinus strobus in inland areas during 9000-8000 ybp, and also led to the expansion of Carya across the coastal part of the region beginning at 7000-6000 ybp. Abrupt cooling at 5500-5000 ybp caused sharp declines in Tsuga in inland areas and Quercus at some coastal sites, and the populations of those taxa remained low until they recovered around 3000 ybp in response to rising precipitation. Throughout most of the Holocene, sites underlain by sandy glacial deposits were occupied by Pinus rigida and Quercus. Main conclusions Postglacial changes in the composition and spatial pattern of New England forests were controlled by long-term trends and abrupt shifts in temperature and precipitation, as well as by
Lake Sediment Pollen and Charcoal from Sears Pond on Long Island NY from 10690 BP to Present
Aim We analyzed a dataset composed of multiple palaeoclimate and lake-sediment pollen and charcoal records from New England to explore how postglacial changes in forest composition and spatial patterns of vegetation and fire were controlled by regional-scale climate change, a subregional environmental gradient, and landscape-scale variations in soil characteristics. Location The 120,000-km2 study area includes parts of Vermont and New Hampshire in the north, where sites are 150-200 km from the Atlantic Ocean, and spans the coastline from southeastern New York to Cape Cod and the adjacent islands, including Block Island, the Elizabeth Islands, Nantucket, and Martha’s Vineyard. Results Boreal forest featuring Picea and Pinus banksiana was present across the region when conditions were cool and dry 14,000-12,000 calibrated 14C yrs before present (ybp). Pinus strobus became regionally dominant as temperatures increased between 12,000 and 10,000 ybp. The composition of forests in inland and coastal areas diverged in response to further warming after 10,000 ybp, when Quercus and Pinus rigida expanded across southern New England, while conditions remained cool enough in inland areas to maintain Pinus strobus. Fire severity was high during 10,000-8000 ybp. Increasing precipitation allowed Tsuga canadensis, Fagus grandifolia, and Betula to replace Pinus strobus in inland areas during 9000-8000 ybp, and also led to the expansion of Carya across the coastal part of the region beginning at 7000-6000 ybp. Abrupt cooling at 5500-5000 ybp caused sharp declines in Tsuga in inland areas and Quercus at some coastal sites, and the populations of those taxa remained low until they recovered around 3000 ybp in response to rising precipitation. Throughout most of the Holocene, sites underlain by sandy glacial deposits were occupied by Pinus rigida and Quercus. Main conclusions Postglacial changes in the composition and spatial pattern of New England forests were controlled by long-term t
Witness Tree Data in Southern New England and Long Island 1640-1999
Early surveyors' witness-tree records from initial land divisions are often used to reconstruct the vegetation at the time of European settlement and before extensive Euro-American land use. The following description of methods comes from the published paper (Hall, B., G .Motzkin, D. R. Foster, M. Syfert and J. Burk. 2002. Three hundred years of forest and land-use change in Massachusetts, USA. Journal of Biogeography 29, 1319-1335): "Following methods described more fully in Cogbill et al. (2002), we used early surveyors' tree records from initial land divisions in each town to reconstruct the vegetation at the time of European settlement and before extensive Euro-American land use. All available original 17th - early 19th century survey records located at the state archives and individual town halls were examined and colloquial or common names of marker trees were noted... With few exceptions, only surveys that occurred within 80 years of town settlement were gathered in order to limit the potential influence of European land use on species composition."
Rates of benthic metabolism and nutrient cycling in the Parker and Rowley Rivers of the Plum Island Sound estuary, Massachusetts, PIE LTER.
Rates of benthic metabolism and nutrient cycling in the Parker and Rowley Rivers of the Plum Island Sound estuary. Measurements include those conducted at two sites in the Parker River in Spring (high river discharge) and Fall (low discharge) for long-term monitoring, also at other sites throughout the estuary over a variety of seasons and salinities.
PIE LTER dissolved nutrient and particulate concentrations of freshwater inputs to the Plum Island estuarine system, Massachusetts, taken approximately monthly.
Multi-year data of water chemistry including nutrient concentrations for various forms of N, P, C, as well as suspended sediments, was determined from monthly grab samples taken at watershed inputs to the Plum Island Sound Estuary. Sampling sites were the Ipswich River (Sylvania Dam, Ipswich, MA), Parker River Dam (Central St, Newbury, MA), Egypt River (Ipswich, MA) Mill River (Newbury, MA), Muddy Run (Ipswich, MA), Little River (Newbury, MA). These nutrient concentrations are then used in conjunction with USGS discharge data (recorded at gages in the Parker River at Byfield, MA and the Ipswich River at Ipswich, MA) to calculate annual nutrient loading to the Plum Island Sound Estuary, coming over each dam. Annual yield is also calculated for both dams. Refer to file WAT-VA-Load for loading data.
PIE LTER transects of the Parker River Plum Island Sound Estuary, Massachusetts conducted at dawn and dusk, containing dissolved oxygen, conductivity, temperature, percent saturation, pH, DIC and pCO2 data.
Multi-year transects, beginning in 1995, of the Parker River Plum Island Sound Estuary conducted at dawn and dusk, containing dissolved oxygen, conductivity, temperature, percent saturation, pH, salinity, DIC and pCO2 measurements. Differences between dawn and dusk measurements can be used to determine the water metabolism and corresponding estimates of gross primary production, total system respiration and net ecosystem production.
Transects of the Rowley River, Plum Island Sound Estuary, Massachusetts conducted at dawn and dusk, containing dissolved oxygen, conductivity, temperature, percent saturation, pH, DIC and pCO2 data, PIE LTER.
Multi-year transects, beginning in 2016, of the Rowley River Plum Island Sound Estuary, MA conducted at dawn and dusk, containing dissolved oxygen, conductivity, temperature, percent saturation, pH, DIC and pCO2 measurements. Differences between dawn and dusk measurements can be used to determine the water column metabolism and corresponding estimates of gross primary production, total system respiration and net ecosystem production.
PIE LTER 10-minute marsh water table height at Nelson Island, Rowley, MA from April-November 2025.
Measurements of water table height in the Nelson Island marsh located near the Nelson Island eddy flux tower, Rowley, MA. Measurements were taken every 10 minutes at each logger along a transect of water level loggers running perpendicular to the Nelson stream bank at Nelson Island from Apr-November 2025.
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.