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64 results for “Lamella”
SPACEtomo training dataset for lamella detection using YOLOv8
<p><strong>Training data used to train a YOLOv8 model for cryoFIB-milled lamella detection on whole grid TEM montages.</strong></p> <p>This dataset contains 614 LM map pieces of varying pixel sizes (rescaled to 400 nm/pixel) containing 1076 examples of lamellae. Lamellae were classified into "good", "contaminated", "thick" and "broken" lamellae. An additional 534 LM map pieces not containing any lamellae were added to the training dataset. To enhance rotational invariance, the 614 LM map pieces were added to the training dataset again with flipped axes.</p> <p>Thanks to <span>Matthias </span><span>P</span><span>ö<span>ge</span></span><span>, Gregor Weiss, Sven Klumpe, <span>Anna Bieber</span><span> and</span><span> Cristina Capitanio for providing whole grid TEM maps.</span></span></p>
FIgS. 26–29. Urodacus butleri, n. sp., paratype ♂ (WAM T85141). 26. Dextral hemispermatophore, dorsal aspect. 27–29. Detail of capsule lamellae, dorsal, ental, and ventral aspects. Abbreviations: Al, anterior lobe; C, capsule; Cp, conical process; DTR, distal transverse ridge; L, lamella; Lh, lamellar hook; Lhp, lamellar hook process; IBL, internobasal lobe; T, trunk. Scale bars = 0.5 mm.
FIgS. 26–29. Urodacus butleri, n. sp., paratype ♂ (WAM T85141). 26. Dextral hemispermatophore, dorsal aspect. 27–29. Detail of capsule lamellae, dorsal, ental, and ventral aspects. Abbreviations: Al, anterior lobe; C, capsule; Cp, conical process; DTR, distal transverse ridge; L, lamella; Lh, lamellar hook; Lhp, lamellar hook process; IBL, internobasal lobe; T, trunk. Scale bars = 0.5 mm.
Fig. 1 in Morphological requirements in limulid and decapod gills: A case study in deducing the function of lamellipedian exopod lamellae
Fig. 1. Dorsal (A) and ventral (B) morphology of Limulus polyphemus.
MicroED datasets from a proteinase K lamella using a 50 micrometre C2 condenser aperture
<p>These are the two diffraction data sets used to determine the proteinase K structure from a crystalline lamella by electron diffraction, as deposited in the PDB with id <a href="http://doi.org/10.2210/pdb6ZEU/pdb">6ZEU</a> and published in <a href="https://doi.org/10.3389/fmolb.2020.00179">https://doi.org/10.3389/fmolb.2020.00179</a></p> <p>A script is provided that allows indexing of the diffraction spots using DIALS 3.1. This is adapted from the commands used to process the data for the publication, which used DIALS 1.10.</p>
Figure 3. Spesbona angusta, lamellae. a in Larva of one of the world's rarest and most threatened damselflies: Spesbona angusta (Odonata: Platycnemididae)
Figure 3. Spesbona angusta, lamellae. a − lat- eral lamella with more complex frills; b − lat- eral lamella with less complex frills; c − me- dial lamella.
FIGURES 14–15. Supracheliceral lamellae. 14 in Nemaspela ladae sp. n., a new troglobitic nemastomatid (Opiliones, Dyspnoi, Nemastomatidae) from a Dinaric cave
FIGURES 14–15. Supracheliceral lamellae. 14, Nemaspela ladae sp. n., male paratype. 15, Hadzinia karamani (Hadži, 1940), male.
FIGURE 7. A. Eurhopalothrix pilulifera, showing expanded infradental lamella. B. E in A review of the Central American and Caribbean species of the ant genus Eurhopalothrix Brown and Kempf, 1961 (Hymenoptera, Formicidae), with a key to New World species
FIGURE 7. A. Eurhopalothrix pilulifera, showing expanded infradental lamella. B. E. xibalba, showing narrow infradental lamella.
FIGURE 10. Lamella copulatrix. a in Taxonomic revision of the Dichotomius sericeus (Harold, 1867) species group (Coleoptera: Scarabaeidae: Scarabaeinae)
FIGURE 10. Lamella copulatrix. a—Dichotomius sericeus (Harold, 1867); b—D. irinus (Harold, 1867); c—D. laevicollis (Felsche, 1901); d—D. schiffleri Vaz-de-Mello, Gavino & Louzada, 2001; e—Dichotomius guaribensis sp. nov.; f— Dichotomius gilletti sp. nov.; g—Dichotomius iannuzziae sp. nov.; h—Dichotomius catimbau sp. nov. Scale bar 0,5 mm.
Supporting MD trajectories for the paper "In Situ Captured Antibacterial Action of Membrane-Incising Peptide Lamellae"
<p>Source data and molecular dynamic trajectories for the article "In Situ Captured Antibacterial Action of Membrane-Incising Peptide Lamellae"</p> <p>New compounds with unique mechanisms of action are needed to combat the growing issue of antimicrobial resistance. Supramolecular assemblies, which combine the complex membrane attacking mechanisms of natural host defense peptides with the improved biostability of non-natural compounds offer a promising alternative to current small molecule antibiotics. However, for such membrane-targeting compounds the direct visual insight on the toxic agents in bacteria is still lacking. To this end, we employed a design strategy focusing on an inducible assembly mechanism and utilized electron microscopy (EM) to follow the formation of supramolecular peptide structures triggered by bacterial cell surface lipopolysaccharides (LPS). Inspired by the alternating chirality backbone pattern of some effective peptide antimicrobials, we designed lysine-rich heterochiral β3-peptides, termed lamellin-2K and lamellin-3K, with optimal residual spacing for enhanced coordination on the phosphate groups of LPS. Combined molecular dynamics simulations (MD), EM and bacterial assays confirmed that the phosphate-induced conformational change of these lamellins led to the formation of thin, striped lamellar layers, where each stripe represents double arrays of H-bonded peptide molecules that are interconnected by phosphate ions. EM micrographs of Gram-negative bacteria show that the lamellae incised the cell envelope, while leakage and antibacterial activity assays prove that growth inhibition starts already at submicromolar concentrations. Detailed image analysis demonstrated that the lamellae penetrating deep into the bacterial cell have a rather uniform size distribution and, surprisingly, only a few of these supramolecules are sufficient to cause major cell wall damage making them efficient in destroying target cells. Our findings also provide a missing mechanistic link for membrane-targeting agents, connecting how the antibiotic mechanism is built up from individual molecules through on-site formation of the active supramolecules that lead to bactericidal activity.</p> <p>Molecular dynamics trajectories from the production runs of single beta-peptide strands in water + 150 mM NaCl + 50:1 MePO4^(2-):peptide. Altogether 8 runs were performed, each one starting from a different helical conformation (H10, H12 and H14, both positive and negative winding) of the peptide. In the first half (500 ns) of the simulation, the intra-chain hydrogen bonds responsible for the actual helix were kept together by distance restraints. In the second half (again 500 ns), these restraints were instantaneously lifted and the system was left to evolve from the same state. In all cases the helical structure unwound in a very short time (under 100 ns), and did not refold in any helix.</p>
FIGURE 2. Phylloporus septocystidiatus. a. basidia. b spores. c hymenophoral trama. d. lamella edge. e. multiseptate cystidia. f–g. pileipellis. h–i in A new species of Phylloporus (Agaricales, Boletaceae) from India
FIGURE 2. Phylloporus septocystidiatus. a. basidia. b spores. c hymenophoral trama. d. lamella edge. e. multiseptate cystidia. f–g. pileipellis. h–i. caulocystidia. Scale bar = 10 μm. Photos: C.K. Pradeep
FIGURE 6 in New species of Mycena (Mycenaceae, Agaricales) with colored lamellae and three new species records from China
FIGURE 6. RAxML and MrBayes phylogram inferred from partial ITS sequence data. The tree is rooted with several species of sect. Calodontes. Maximum Likelihood support values (>90) and posterior probalities (>0.90) are showed on each branches (MP/PP). New and newly recorded species are marked by ●.
FIGURE 5 in New species of Mycena (Mycenaceae, Agaricales) with colored lamellae and three new species records from China
FIGURE 5. Mycena strobilinoidea (HMJAU 43654) a. Basidiomata; b. Basidia; c. Basidiospores; d. Cheilocystidia; e. Pleurocystidia; f. Pileipellis; g. Stipitipellis. Bars a=1 cm; b, c, d, e, f, g=10 μm Illustration by Qin Na
FIGURE 2 in New species of Mycena (Mycenaceae, Agaricales) with colored lamellae and three new species records from China
FIGURE 2. Mycena entolomoides (HMJAU 43048) a. Basidiomata; b. Basidia; c. Basidiospores; d. Pleurocystidia; e. Cheilocystidia; f. Pileipellis; g. Stipitipellis. Bars a=1 cm; b, c, d, e, f, g=10 μm Illustration by Qin Na
FIGURE 4 in New species of Mycena (Mycenaceae, Agaricales) with colored lamellae and three new species records from China
FIGURE 4. Mycena purpureofusca (HMJAU 43554) a. Basidiomata; b. Basidiospores; c.; Basidia d. Pileipellis; e. Stipitipellis; f. Cheilocystidia. Bars a=2 cm; b, c, d, e, f=10 μm Illustration by Qin Na
FIGURE 1. a in New species of Mycena (Mycenaceae, Agaricales) with colored lamellae and three new species records from China
FIGURE 1. a) Mycena entolomoides (HMJAU 43048) b) M. entolomoides (HMJAU 43052) c, d) M. citrinomarginata (HMJAU 43563) e) M. purpureofusca (HMJAU 43554) f) M. purpureofusca (HMJAU 43624) g, h) M. strobilinoidea (HMJAU 43654). Bars: a-h=10 mm. Photos a–b by Yu-Guang Fan; Photos c–h by Qin Na
FIGURE 3 in New species of Mycena (Mycenaceae, Agaricales) with colored lamellae and three new species records from China
FIGURE 3. Mycena citrinomarginata (HMJAU 43563) a. Basidiomata; b. Basidia; c. Basidiospores; d. Cheilocystidia; e. Pileipellis; f. Stipitipellis. Bars a=1 cm; b, c, d, e, f =10 μm Illustration by Qin Na
FIGURE 1 in Xanthagaricus siamensis sp. nov. (Agaricaceae), a new species with dull green lamellae from northern Thailand
FIGURE 1. RAxML tree based on a combined ITS and nrLSU sequence dataset. Bootstrap values for ML and MP equal or greater than 75% are placed above and below the branches respectively. Branches with Bayesian posterior probabilities (PP) from MCMC analysis equal or greater than 0.95 are in bold. Newly generated sequences are indicated in red. The tree is rooted with Chlorophyllum globosum (DMSC1138) and C. molybdites (DMSC07290).
FIGURE 3 in Xanthagaricus siamensis sp. nov. (Agaricaceae), a new species with dull green lamellae from northern Thailand
FIGURE 3. Xanthagaricus siamensis. (MFLU 19-0575, holotype). a: Basidiospores. b: Basidia. c: Cheilocystidia. d: Pileipellis hyphae. Scale bars: a=2 μm, b–c=4 μm, d=20 μm.
FIGURE 2 in Xanthagaricus siamensis sp. nov. (Agaricaceae), a new species with dull green lamellae from northern Thailand
FIGURE 2. Fresh basidiomata of Xanthagaricus siamensis at different stages of development. a–e: MFLU 19-0574. f–g: MFLU 19-0575 (holotype). h: MFLU 19-0576. Scale bars: a–e=2 cm, f–g=1 cm, h=2 cm.
FIGURES 246–248. Elaphropeza ephippiata. 246. epandrium with cerci. 247. right epandrial lamella. 248 in Revision of the genus Elaphropeza Macquart (Diptera: Hybotidae) from the Oriental Region, with a special attention to the fauna of Singapore
FIGURES 246–248. Elaphropeza ephippiata. 246. epandrium with cerci. 247. right epandrial lamella. 248. left surstylus.
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