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24 results for “Landscape Survey”
Data from: Survey sequencing reveals elevated DNA transposon activity, novel elements, and variation in repetitive landscapes among vesper bats
The repetitive landscapes of mammalian genomes typically display high Class I (retrotransposon) transposable element (TE) content, usually around half of the genome. In contrast, the Class II (DNA transposon) contribution is typically small (<3% in model mammals). Most mammalian genomes also exhibit a precipitous decline in Class II activity beginning roughly 40 million years ago (Ma). The first signs of more recently active mammalian Class II TEs were obtained from the little brown bat, Myotis lucifugus and are reflected by higher genome content (~5%). To aid in determining taxonomic limits and potential impacts of this elevated Class II activity, we performed 454 survey sequencing of a second Myotis species as well as four additional taxa within the family Vespertilionidae and an outgroup species from Phyllostomidae. Graph-based clustering methods were used to reconstruct the major repeat families present in each species and novel elements were identified in several taxa. Retrotransposons remained the dominant group with regard to overall genome mass. Elevated Class II TE composition (3-4%) was observed in all five vesper bats while less than 0.5% of the phyllostomid reads were identified as Class II derived. Differences in satellite DNA and Class I TE content are also described among vespertilionid taxa. These analyses present the first cohesive description of TE evolution across closely related mammals, revealing genome-scale differences in TE content within a single family.
Landscape scale lake surveys of Algonquin Provincial Park
<p>To better understand the status of Lake Trout and Brook Trout populations across the Algonquin Provincial Park landscape 192 index netting surveys were conducted on 161 lakes utilizing standardized multimesh benthic gillnets between the years 2009 and 2022.</p> <p>Index netting was conducted using a depth-stratified randomized site survey design and employed multi-pass sampling on a majority of the lakes to provide the opportunity for occupancy analysis. Two primary netting methods were employed; <a href="https://www.ontario.ca/page/summer-profundal-index-netting-manual" rel="noopener">Summer Profundal Index Netting (SPIN)</a> for Lake Trout populations sampled between 2009-2012; and a modified <a href="https://www.ontario.ca/page/broad-scale-fish-community-monitoring" rel="noopener">Ontario Broadscale Monitoring</a> (BsM) method we refer to as Short Duration Point Sampling (SDPS). The SDPS method uses the large mesh BsM nets (NA1) deployed for a one-hour duration within the same depth strata used in the BsM program with a sampling intensity in each stratum proportional to the surface area. The overall sampling intensity (nets/lake) is greater than that employed in the BsM program as we are interested in lake-specific analyses. </p> <p>This data dryad provides general information on each lake sampled including lake characteristics, lake volumes, netting site locations, and spring water chemistry. </p>
Landscape scale lake surveys of Algonquin Provincial Park
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Data from: Survey sequencing reveals elevated DNA transposon activity, novel elements, and variation in repetitive landscapes among vesper bats
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