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191 results for “Low flows”
dataset for "Corner-bridge flow as pore-scale flow mechanism controlling capillary pressure curve at low saturations"
<p>dataset for "Corner-bridge flow as pore-scale flow mechanism controlling capillary pressure curve at low saturations", including raw images from drainage experiments</p>
A streamlined approach for fluorescence labelling of low copy-number plasmids for determination of conjugation frequency by flow cytometry
<p><span>Bacterial conjugation plays a major role in the dissemination of antibiotic resistance and virulence traits through horizontal transfer of plasmids. </span>Robust <span>measurement</span> of<span> the conjugation frequency of plasmids between bacterial strains and species </span>is therefore important <span>to understand the transfer dynamics </span>and epidemiology <span>of conjugative plasmids. In this study, we present a streamlined experimental approach for fluorescence labelling of low copy-number conjugative plasmids that allows plasmid transfer frequency during filter mating to be measured by flow cytometry. A blue fluorescence gene is inserted into a conjugative plasmid of interest using a simple homologous recombineering procedure. </span><span>A small non-conjugative plasmid, which carries a red fluorescence gene with a toxin-antitoxin system that functions as a plasmid stability module, is used to label the recipient bacterial strain. This offers the dual advantage of circumventing chromosomal modifications of recipient strains and ensuring that the red fluorescence gene-bearing plasmid can be stably maintained in recipient cells in an antibiotic-free environment during conjugation. A strong constitutive promoter allows the two fluorescence genes to be strongly and constitutively expressed from the plasmids, thus allowing flow cytometers to clearly distinguish between donor, recipient and transconjugant populations in a conjugation mix for monitoring conjugation frequencies more precisely over time. </span></p>
Psycho-physio-neurological correlates of qualitative attention, emotion and flow experiences in a close-to-real-life extreme sports situation: low- and high-altitude slackline walking
<p>It has been indicated that extreme sport activities, despite providing fear, stress and anxiety, also result in a highly rewarding experience. Studies have related this experience to the concept of flow, a positive feeling that individuals undergo when they are completely immersed in an activity. However, little is known about the exact nature of these experiences, and, there are still no empirical results to characterize the brain dynamics during extreme sport practice. This work aimed at investigating changes in psychological responses while recording physiological (heart rate – HR, and breathing rate – BR) and neural (electroencephalographic – EEG) data of eight volunteers, during outdoors slackline walking in a mountainous environment at two different altitude conditions (1 m – low-walk – and 45 m – high-walk – from the ground). Low-walk showed a higher score on flow scale, while high-walk displayed a higher score in the negative affect aspects, which together point to some level of flow restriction during high-walk. The order of task performance was shown to be relevant for the physiological and neural variables. The brain behavior during flow, mainly considering attention networks, displayed the stimulus-driven ventral attention network – VAN, regionally prevailing (mainly at the frontal lobe), over the goal-directed dorsal attention network – DAN. Therefore, we suggest an interpretation of flow experiences as an opened attention to more changing details in the surroundings, i.e., configured as a ‘task-constantly-opened-to-subtle-information experience’, rather than a ‘task-focused experience’.</p>
Psycho-physio-neurological correlates of qualitative attention, emotion and flow experiences in a close-to-real-life extreme sports situation: low- and high-altitude slackline walking
<p>It has been indicated that extreme sport activities, despite providing fear, stress and anxiety, also result in a highly rewarding experience. Studies have related this experience to the concept of flow, a positive feeling that individuals undergo when they are completely immersed in an activity. However, little is known about the exact nature of these experiences, and, there are still no empirical results to characterize the brain dynamics during extreme sport practice. This work aimed at investigating changes in psychological responses while recording physiological (heart rate – HR, and breathing rate – BR) and neural (electroencephalographic – EEG) data of eight volunteers, during outdoors slackline walking in a mountainous environment at two different altitude conditions (1 m – low-walk – and 45 m – high-walk – from the ground). Low-walk showed a higher score on flow scale, while high-walk displayed a higher score in the negative affect aspects, which together point to some level of flow restriction during high-walk. The order of task performance was shown to be relevant for the physiological and neural variables. The brain behavior during flow, mainly considering attention networks, displayed the stimulus-driven ventral attention network – VAN, regionally prevailing (mainly at the frontal lobe), over the goal-directed dorsal attention network – DAN. Therefore, we suggest an interpretation of flow experiences as an opened attention to more changing details in the surroundings, i.e., configured as a ‘task-constantly-opened-to-subtle-information experience’, rather than a ‘task-focused experience’.</p>
Low-Load Blood Flow Restriction Training vs Traditional Resistance Training Exercises Following ACLR Surgery
ClinicalTrials.gov study NCT06480032. IPD Sharing: YES. Countries: 1. Publications: 3.
Low Load Resistance Training Using Blood Flow Restriction for People With Multiple Sclerosis
ClinicalTrials.gov study NCT04633759. IPD Sharing: Not stated. Countries: 1. Publications: 1.
The Impact of Low Flow Nocturnal Oxygen Therapy on Hospital Admissions and Mortality in Patients With Heart Failure and Central Sleep Apnea
ClinicalTrials.gov study NCT03745898. IPD Sharing: YES. Countries: 1. Publications: 1.
Is Low-load Resistance Training With Blood Flow Restriction Feasible During Rehabilitation of Military Personnel With Lower Limb Injuries? Phase One RCT.
ClinicalTrials.gov study NCT06621914. IPD Sharing: YES. Countries: 1. Publications: 1.
Low Intensity Blood Flow Restriction Training Study
ClinicalTrials.gov study NCT03055260. IPD Sharing: YES. Countries: 1. Publications: 2.
Is Low-load Resistance Training With Blood Flow Restriction More Effective Than Traditional Rehabilitation of Military Personnel With Lower Limb Injuries
ClinicalTrials.gov study NCT06621953. IPD Sharing: YES. Countries: 1. Publications: 1.
The Efficacy of Low-load Blood Flow Restricted Resistance Before TKR
ClinicalTrials.gov study NCT04081493. IPD Sharing: YES. Countries: 1. Publications: 3.
A streamlined approach for fluorescence labelling of low copy-number plasmids for determination of conjugation frequency by flow cytometry
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Data from: Anthropogenic land‐use change intensifies the effect of low flows on stream fishes
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Data and scripts from: Balanced polymorphism fuels rapid selection in an invasive crab despite high gene flow and low genetic diversity
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Dataset to accompany: Heat flux in low mass flux horizontal cryogenic flow
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Data from: Low coverage genomic data resolve the population divergence and gene flow history of an Australian rain forest fig wasp
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Data from: Experimentally induced low flows indicate climate change may shrink trophic niches of mountain-stream predators
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Importance of snowmelt contribution to seasonal runoff and summer low flows in Czechia
<p>The file in this record represents a supplementary data for the journal paper Jenicek, M., Ledvinka, O. (2020). Importance of snowmelt contribution to seasonal runoff and summer low flows in Czechia. Hydrology and Earth System Sciences, https://doi.org/10.5194/hess-2019-611. The file contain simulations of the HBV rainfall-runoff model for 59 mountain catchments in Czechia. The model simulated different water balance components, such as runoff, snow water equivalent, evapotranspiration and groundwater storage for the study period 1980-2014.</p>
Data from: Gene flow in the European coal tit, Periparus ater (Aves: Passeriformes): low among Mediterranean populations but high in a continental contact zone
Extant phylogeographic patterns of Palearctic terrestrial vertebrates are generally believed to have originated from glacial range fragmentation. Post-Pleistocene range expansions have led to the formation of secondary contact zones among genetically distinct taxa. For coal tits (Periparus ater), such a contact zone has been localized in Germany. In this study, we quantified gene flow between Fennoscandian and southern European coal tits using a set of 13 microsatellite loci. STRUCTURE analysis revealed four genetic clusters two of these on Mediterranean islands. German populations were genetically admixed but introgression of southern alleles was evident for Fennoscandian populations. In the South, we found negligible introgression of northern alleles (and haplotypes) but slight admixture of two southern genetic clusters in the Pyrenees and on the Balkan Peninsulae and near complete sorting of these two allelic lineages on the islands of Corsica and Sardinia. Genetic distinctiveness of the Mediterranean island populations reflects general patterns of endemism in the Corso-Sardinian fauna and the Cypriot fauna. Wide-range gene flow in Central Europe suggests a broad zone of intergradation between subspecies of the coal tit rather than a narrow contact zone. This is in accordance with low morphological and bioacoustic differentiation of European coal tit populations.
Data from: Run to the hills: gene flow among mountain areas leads to low genetic differentiation in the Norwegian lemming
The endemic Norwegian lemming (Lemmus lemmus) is an icon for cyclic species, famous since the Middle Ages for its enormous population outbreaks and mass movements. Although the drivers behind this cyclicity have been intensively investigated, virtually nothing is known about the extent to which long-distance dispersal during population peaks actually lead to gene flow among mountain tundra areas. In this article, we use nine microsatellite markers to address this question and analyse range-wide genetic diversity and differentiation between Fennoscandian sub-regions. The results revealed a high genetic variation with a surprisingly weak population structure, comparable to that of much larger mammals. The differentiation was mainly characterized as a genetic cline across the species' entire distribution, and results from spatial autocorrelation analyses suggested that gene flow occurs with sufficiently high frequency to create a genetic patch size of 100 km. Further, we found that for the equivalent distances, the southern sub-regions were genetically more similar to each other than those in the north, which indicates that the prolonged periods of interrupted lemming cyclicity recorded in the northern parts of Fennoscandia have led to increased isolation and population differentiation. In summary, we propose that mass movements during peak years act as pulses of gene flow between mountain tundra areas, and that these help to maintain genetic variation and counteract differentiation over vast geographic distances.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.