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datasets available to search
ShareScore release 0.9.0
Dataset results
37 results for “Luciferase”
Next-generation interaction screening to discover luciferase (construct #1) related protein-protein interactions regulating barley powdery mildew disease immunity and susceptibility
GEO Series GSE164761. Hordeum vulgare; Blumeria hordei. 6 samples. Type: Other.
RNA-seq of Lin-CD34+ cord-blood cells overexpressing PLAG1-S or firefly Luciferase [OE]
GEO Series GSE181976. Homo sapiens. 4 samples. Type: Expression profiling by high throughput sequencing.
Gene expression profile of UOK257 cells silenced for TFEB or TFE3 relative to control UOK257 silenced for luciferase
GEO Series GSE207650. Homo sapiens. 11 samples. Type: Expression profiling by high throughput sequencing.
RNA-seq analysis of Luciferase control-infected and DOT1L shRNA-infected primary cortical neurons.
GEO Series GSE272883. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
Expression changes in HeLa cells treated with siRNA against HCFC1 or control luciferase
GEO Series GSE31412. Homo sapiens. 18 samples. Type: Expression profiling by array.
Data from: Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy
Secreted Nano-luciferase (secNluc) is a newly engineered secreted luciferase that possesses advantages of high structural stability, long half-life, and glow-type kinetics together with high light emission intensity, and thus would become one of the most valuable tools for bioluminescence assays. However, like other secreted luciferases, secNluc has to mix with the components in the conditioned medium surrounding test cells, or in the biological samples such as blood or urine after being secreted. These components may interfere with secNluc-catalyzed bioluminescence reactions and thus limit the application of the secNluc reporter system. In this study, we first examined the effects of three factors, pH, serum and residual reagents, on secNluc-catalyzed bioluminescence reactions, finding that these factors could interfere with bioluminescence reactions and result in background signal. To resolve these problems, we applied a simple affinity purification strategy in which secNluc was fused with a FLAG-tag, and anti-FLAG magnetic beads were used to catch and transfer the fusion protein to PBST, an optimal buffer for secNluc-catalyzed bioluminescence reactions that was identified in this study. The results indicated that this strategy could not only negate the interferences from serum or residual reagents and enhance the stability of light emission but also greatly increase signal intensity through enzyme enrichment. This strategy may contribute to biomedical studies that utilize secNluc and other secreted luciferases, especially those requiring superior sensitivity, low background noise and high reproducibility.
Normalised luciferase assay data
<p>The Lepidoptera are an insect order of cultural, economic, and environmental importance, representing ∼10% of all described living species. Yet, for all but one of these species (silkmoth, <em>Bombyx mori</em>), the molecular genetics of how sexual fate is determined remains unknown. We investigated this in the diamondback moth (<em>Plutella xylostella</em>), a globally important, highly invasive, and economically damaging pest of cruciferous crops. Our previous work uncovered a regulator of male sex determination in <em>P. xylostella</em>—<em>PxyMasc</em>, a homolog of <em>B. mori Masculinizer</em>—which, although initially expressed in embryos of both sexes, is then reduced in female embryos, leading to <span class="PI"></span>female-specific<span class="PI"></span> splicing of <em>doublesex</em>. Here, through sequencing small RNA libraries generated from early embryos and sexed larval pools, we identified a variety of small silencing RNAs (predominantly <span class="PI"></span>Piwi-interacting<span class="PI"></span> RNAs [piRNAs]) complementary to <em>PxyMasc</em>, whose temporal expression correlated with the reduction in <em>PxyMasc</em> transcript observed previously in females. Analysis of these small RNAs showed that they are expressed from tandemly arranged, multicopy arrays found exclusively on the W (female-specific) chromosome, which we term "<em>Pxyfem</em>". Analysis of the <em>Pxyfem</em> sequences showed that they are partial complementary DNAs (cDNAs) of <em>PxyMasc</em> messenger RNA (mRNA) transcripts, likely integrated into transposable element graveyards by the noncanonical action of retrotransposons (retrocopies), and that their apparent similarity to <em>B. mori feminizer</em> more probably represents convergent evolution. Our study helps elucidate the sex determination cascade in this globally important pest and highlights the "shortcuts" that retrotransposition events can facilitate in the evolution of complex molecular cascades, including sex determination.</p>
Molecular modeling of luciferyl adenylate deprotonation in the active site of Photinus pyralis luciferase
<p>Supporting material for the paper</p>
Effects of LINE-1 induction on transcription in 4T1 cells carrying transgenic recombinant cassette with doxycyclin-inducible LINE-1 with a downstream Gaussia luciferase
GEO Series GSE217039. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
Normalised luciferase assay data
Open the record for dataset details and reuse information.
Data from: Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy
Open the record for dataset details and reuse information.
DNA methylation analysis of HCT116 after knockdown of LIG1, UHRF1, or luciferase (control)
GEO Series GSE147518. Homo sapiens. 3 samples. Type: Methylation profiling by genome tiling array.
MCF-7 Luciferase, PARP-1, PARG, SIRT1, and macroH2A Knockdown
GEO Series GSE12971. Homo sapiens. 15 samples. Type: Expression profiling by array.
High-throughput sensitive screening of small molecule modulators of microexon alternative splicing using dual Nano and Firefly luciferase reporters
GEO Series GSE228599. Mus musculus. 34 samples. Type: Expression profiling by high throughput sequencing.
A Split Luciferase Biosensing Platform for Detection and Imaging of Chromatin Loops in Individual Live Cells
GEO Series GSE295357. Homo sapiens. 12 samples. Type: Other.
Luciferase Expressing Preclinical Model Systems Representing the Different Molecular Subtypes of Colorectal Cancer
GEO Series GSE241569. Homo sapiens. 27 samples. Type: Expression profiling by array.
Gene expression profiles of SOX17 overexpression in the KYSE170 luciferase cell
GEO Series GSE35975. Homo sapiens. 2 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.