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Figure 6 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 6. Karyogram of (A) Dugesia benazzii s.s. from Su Rizzolu River (Oưi, loc. 13) and (B) Dugesia hoidi.

Figure 1. A in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 1. A, sampling localities of the present study. Numbers correspond to population codes listed in Appendix, Table A1 and coincide with those in Dols-Serrate et al. (2020). Red circles indicate populations used for morphological analyses. B, rectangular inset: enlargement of the Bunnari–Mascari confluence area. Ŋe map was created using Q-GIS v.3.2.2 (hưps://qgis.org/es/site/ last accessed September 2023) and edited in ILLUSTÞTOR CC v.22.0.1 (hưps://www.adobe.com/products/illustrator.html last accessed September 2023).

Figure 14 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 14. Dugesia mariae: A, CGAS Pla 27. 1, photomicrograph showing the penis bulb with the seminal vesicle (sv), less (lvd) and right (rvd) vas deferens, the penial papilla (pp) and the two atrial folds (af) in horizontal section; B, CGAS Pla 27. 4, photomicrograph showing the penis papilla (pp) and the penial fold (pf) in transverse section.

Figure 9. Dugesia benazzii s.s in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 9. Dugesia benazzii s.s.: A, CGAS Pla 25. 6, photomicrograph showing the penial fold (pf) and the two atrial folds (af) in sagiưal section; B, CGAS Pla 25. 4, photomicrograph showing the penis papilla (pp) and the penial fold (pf) in transverse section.

Figure 3 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 3. mtDNA (dataset I) phylogenetic tree and species discovery delimitation schemes for ABGD, GMYC, and mPTP, as well as PSHs and PSCs. Ultrametric tree from BEAST is shown only for visual purposes; posterior probabilities (pp) and bootstrap support values (bs) relate to MrBayes and ÞxML analyses, respectively; pp and bs node support values represented by squares and circles, filled with white (unsupported), grey (supported), and black (maximum support), respectively.

Figure 8. Dugesia benazzii s.s., CGAS Pla 25.1 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 8. Dugesia benazzii s.s., CGAS Pla 25.1. Photomicrograph of the copulatory bursa with a ciliate parasite (cp) and a spermatophore (sp) in a sagiưal section.

Figure 5 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 5. BFD results for the array of models tested (1–6), with different a priori species-delimitation hypotheses tested on three datasets (I, IV, and V). Each column represents a model with a unique combination of lineages (rows). Star symbol indicates reassignment of PSC4. Marginal-likelihood estimates (MLE) and Bayes' factors comparison results (2lnBf) from the combined analyses of five independent runs with PS (path-sampling) and SS (stepping stone) are represented with circles and a grey-scale scheme. 2lnBf comparison support indicates differences from the best model: non-significant indicates no difference in support for the two models; positively supported and decisively-supported indicate clear support in favour of the best-fiưing model over its alternative.

Figure 4 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 4. Schematic representation of BPP results on multi-locus data for two topologies. Colour scheme and squares represent posterior probability (pp) for each node under four different prior combinations, using two algorithms (A0 and A1) and three datasets (I, IV, and V). *Unsupported node with a pp of 0.94.

Figure 2 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 2. Simplified Bayesian (A–E1) and ML dendrograms (E2). A, dataset I: COI–NADH4–tRNAW–COII; B, dataset II: Dunuc10; C, dataset III: Dunuc12; D, dataset IV: Dunuc10 * 12; E, dataset V: mtDNA * nDNA. Posterior probabilities (pp) are indicated by filled squares and bootstrap support values (bs) with filled circles. Full trees are represented in Figure 3 (dataset I) and Supporting Information, Figure S1 (datasets I–V).

Figure 13. Dugesia mariae. Holotype RMNH.VER.21056.1 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 13. Dugesia mariae. Holotype RMNH.VER.21056.1: A, sagiưal reconstruction of the copulatory apparatus (anterior to the right); B, photomicrograph showing, in a sagiưal section, the penis bulb (pb) with seminal vesicle (sv), penis papilla (pp) with the pointed diaphragm (d), pleated ejaculatory duct (ed), and the penial fold (pf).

Figure 12 in Fantastic beasts and how to delimit them: an integrative approach using multispecies coalescent methods reveals two new, endemic Dugesia species (Platyhelminthes: Tricladida) from Corsica and Sardinia

Figure 12. Dugesia mariae. Photomicrograph of a preserved sexual specimen from the Golo River (Barcheưa, loc. 24).

Figure 6 in Bayesian Poisson tree processes and multispecies coalescent models shed new light on the diversification of Nawab butterflies in the Solomon Islands (Nymphalidae, Charaxinae, Polyura)

Figure 6. Habitus of Polyura bicolor and Polyura epigenes across the Solomon Islands. Pictures of the upperside (left half) and underside (right half) of the two sexes. All pictures taken by Bernard Turlin.

Figure 4 in Bayesian Poisson tree processes and multispecies coalescent models shed new light on the diversification of Nawab butterflies in the Solomon Islands (Nymphalidae, Charaxinae, Polyura)

Figure 4. Nuclear haplotype network and maximum-likelihood phylogenetic reconstruction. The haplotype network reconstructed using the concatenated RPS5 and wingless alignments is presented on the left. The phylogenetic hypothesis inferred using the same data set in RA×ML is presented at the top right. A table highlighting the nucleotide substitutions found between Polyura epigenes bicolor and Polyura epigenes* is presented at the bottom right.

Figure 3 in Bayesian Poisson tree processes and multispecies coalescent models shed new light on the diversification of Nawab butterflies in the Solomon Islands (Nymphalidae, Charaxinae, Polyura)

Figure 3. Comparison of species delimitation results. Graph showing the result of each analysis of species delimitation. The posterior probability of either Polyura bicolor or Polyura epigenes (including Polyura bicolor) as a valid species are shown.

Figure 2 in Bayesian Poisson tree processes and multispecies coalescent models shed new light on the diversification of Nawab butterflies in the Solomon Islands (Nymphalidae, Charaxinae, Polyura)

Figure 2. Molecular phylogeny and molecular species delimitation results. Bayesian phylogeny as recovered from MrBayes analyses. The nodal supports of both Bayesian-inference (BI) and maximum-likelihood (ML) analyses are shown, with colour coding as indicated in the figure. The cloudogram of the *BEAST analysis showing all posterior species trees is presented at the bottom left. Denser regions indicate a robust support for the inferred relationship.

Figure 1 in Bayesian Poisson tree processes and multispecies coalescent models shed new light on the diversification of Nawab butterflies in the Solomon Islands (Nymphalidae, Charaxinae, Polyura)

Figure 1. Map of the geographic range of Polyura epigenes in the Solomon Islands. Male habitus of the different subspecies are shown above the name of the taxon. The distribution of each taxa is indicated by a coloured dashed line. The colour of the line refers to the pastille on the side of the taxon name. All pictures were taken by Bernard Turlin. The map is from National Geographic's MapMaker Interactive.

Figure 5 in Bayesian Poisson tree processes and multispecies coalescent models shed new light on the diversification of Nawab butterflies in the Solomon Islands (Nymphalidae, Charaxinae, Polyura)

Figure 5. Divergence time estimates derived from the *BEAST analysis. Chronogram derived from the posterior trees of dating analysis conducted in BEAST. The 95% credibility intervals are shown at each node of the phylogeny. A map of the Solomon Islands with bathymetry is shown at the top of the figure. The island of Malaita is highlighted in violet. A picture of a female Polyura epigenes bicolor f. cinereus (orange morph) is presented. Picture taken by Bernard Turlin. T in time bar stands for Tarantian.