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openCC0Jan 2022View details →

NEON lakes Level 0 multisonde temperature data - 2021

The National Ecological Observatory Network (NEON; https://www.neonscience.org) collects water temperature measurements from its seven lake sites using a temperature chain consisting of thermistors measuring at various depths throughout the water column. This data is published as part of the water temperature at specific depth in surface water data product (DP1.20264.001). During 2021, a misconfiguration resulted in gaps in this data. The multisonde used to measure water quality (DP1.20288.001) also collects water temperature measurements that can potentially be used to fill these gaps. This data is not published by NEON as part of the Level 1 water quality data product because it is not as accurate as the temperature chain derived measurements. The calibration of the multisonde temperature sensor is also factory set and cannot be adjusted. This data package contains the Level 0 multisonde water temperature data for NEON lake sites in 2021. This is raw data that has not been QA/QC'ed. NEON makes no guarantees about the accuracy of this data.

openCC (other)Apr 2022View details →

NEON Provisional aquatic biology and single aspirated air temperature data 2020-2021

NEON field and taxonomic and abundance data for the periphyton (DP1.20166.001), macroinvertebrate (DP1.20120.001), and zooplankton (DP1.20219.001) collection for all aqatic sites, as well as single aspirated air temperature for 15 sites, were downloaded from the NEON data portal. Provisional data, not included in the 2022 data release, are included in this upload. Data are from all 34 aquatic sites across the network. Data are from NEON sources only. NEON is sponsored by the National Science Foundation (NSF) and operated under a cooperative agreement by Battelle. This material is based in part upon work supported by NSF through the NEON Program. NEON (National Ecological Observatory Network). Periphyton, seston, and phytoplankton collection (DP1.20166.001). https://data.neonscience.org (accessed March 12, 2022) NEON (National Ecological Observatory Network). Macroinvertebrate collection (DP1.20120.001). https://data.neonscience.org (accessed March 12, 2022) NEON (National Ecological Observatory Network). Zooplankton collection (DP1.20219.001). https://data.neonscience.org (accessed March 12, 2022) NEON (National Ecological Observatory Network). (2022e). Single aspirated air temperature (DP1.00002.001). https://data.neonscience.org (accessed March 12, 2022) NEON field and taxonomic and abundance data for the periphyton (DP1.20166.001), macroinvertebrate (DP1.20120.001), and zooplankton (DP1.20219.001) collection were downloaded from the NEON data portal. Provisional data, not included in the 2021 data release, are included in this upload. Data are from all 34 aquatic sites across the network. Data are from NEON sources only. NEON is sponsored by the National Science Foundation (NSF) and operated under cooperative agreement by Battelle. This material is based in part upon work supported by NSF through the NEON Program. NEON (National Ecological Observatory Network). Periphyton, seston, and phytoplankton collection (DP1.20166.001). https://data.neonscience.org (accesse

openCC (other)May 2022View details →

Quantity and composition of POM and MAOM in 156 soil samples collected from 20 National Ecological Observatory Network (NEON) sites in 2019

While it is generally assumed that particulate organic matter (POM) and mineral associated organic matter (MAOM) have distinct biogeochemical characteristics, it remains unresolved where and why POM and MAOM differ in their composition and relationships to total SOM decomposition among heterogenous soils. To address these questions, we analyzed elemental, isotopic, and chemical composition, including diffuse reflectance infrared Fourier transform (DRIFT) spectra, of POM and MAOM in 156 soil samples collected from 20 National Ecological Observatory Network (NEON) sites spanning diverse ecosystems (tundra to tropics) across North America in 2019. We used a classic size separation method for POM (53–2000 µm) and MAOM (< 53 µm) following chemical dispersion.

Blair et al. 2020: Machine learning identification of ground beetles (repackaging of occurrences published by the NEON Biorepository Data Portal)

Blair, J.; Weiser, M. D.; Kaspari, M.; Miller, M.; Siler, C.; Marshall, K. E. 2020. Robust and simplified machine learning identification of pitfall trap-collected ground beetles at the continental scale. Ecology and Evolution 10 (23): 13143-13153. https://doi.org/10.1002/ece3.6905 Additional NEON samples (not yet archived at the Biorepository) were used in this research: full list of occurrences used.

Stachewicz et al. 2021: Trait correlation, phylogenetic signal in Carabidae morphology (repackaging of occurrences published by the NEON Biorepository Data Portal)

Stachewicz JD, Fountain-Jones NM, Koontz A, Woolf H, Pearse WD, Gallinat AS. 2021. Strong trait correlation and phylogenetic signal in North American ground beetle (Carabidae) morphology bioRxiv 02.12.431029; doi: https://doi.org/10.1101/2021.02.12.431029 Many NEON samples and specimens used in this work resulted from NEON prototype data and will not be archived in the Biorepository. See the appendices in the above linked article for a full list of NEON samples and specimens and their associated collection data. Additionally, see appendices of above linked article for specimen-level morphological trait measurements and genetic sequence data.

Staines & Staines 2021: The Geadephaga (Coleoptera: Carabidae and Rhysodidae) of SERC (repackaging of occurrences published by the NEON Biorepository Data Portal)

Linked records of Carabidae and Rhysodidae specimens from the SERC site, collected between 2015-2018, included in the following publication: Staines, C.L. & Staines, S. L. The Geadephaga (Coleoptera: Carabidae and Rhysodidae) of the Smithsonian Environmental Research Center, Maryland. 2021. Banisteria 55: 75-100. Original abstract: "An inventory of the Geadephaga (Coleoptera) at the Smithsonian Environmental Research Center, Anne Arundel County, Maryland is being conducted. Pitfall traps were placed and monitored from 2015 to 2018. From 2017 to 2020 directed collecting efforts were made to document the Geadephaga of the facility. A total of 111 Geadephaga species was collected: Carabidae - 110, Rhysodidae - 1." Research article available for download: https://virginianaturalhistorysociety.com/banisteria/pdf-files/ban55/Staines_SERC_Geadephaga.pdf

Decomissioned Site: Arthur Brook's (D01 ARTH) Legacy and Prototype Aquatic invertebrates (repackaging of occurrences published by the NEON Biorepository Data Portal)

This collection contains legacy and prototype NEON aquatic invertebrates from the decommissioned Arthur Brook's site in Worcester County, Massachusets. These samples and specimens were collected using NEON protocol NEON.DP1.20120 and are summarized in NEON Prototype dataset e7d152a1-1181-4c7e-ac75-ae707ffc7299. This data is available here.

Decomissioned Site: Ichawanochaway Creek (D03 ICHA) Legacy and Prototype Aquatic invertebrates (repackaging of occurrences published by the NEON Biorepository Data Portal)

This collection contains legacy and prototype NEON aquatic invertebrates from the decommissioned Ichawanochaway Creek site in Baker County, Georgia. These samples and specimens were collected using NEON protocol NEON.DP1.20120 and are summarized in NEON Prototype dataset 1599af29-fad4-4721-9b09-f8324b672d50. This data is available here.

Decommissioned Site: Mameyes (D04 MAME) Belowground Plant Biomass (Megapit) (repackaging of occurrences published by the NEON Biorepository Data Portal)

These samples are associated with NEON prototype dataset: NEON Decommissioned Site data: Root sampling, chemistry, and isotopes (Megapit) from D04 MAME site (a36e6ce2-d845-13e9-c881-c1d4e5881a53) DOI: 10.48443/j1hp-f273 NEON (National Ecological Observatory Network). NEON Decommissioned Site data: Root sampling, chemistry, and isotopes (Megapit) from D04 MAME site, v1 (10.48443/j1hp-f273). https://doi.org/10.48443/j1hp-f273. Dataset accessed from https://data.neonscience.org on July 7, 2021

Identified invertebrate bycatch from beetle pitfall traps at SJER and SOAP, 2017 - 2018 (repackaging of occurrences published by the NEON Biorepository Data Portal)

California permit requirements necessitated a more thorough identification of beetle pitfall samples than is typical of this protocol. These invertebrate bycatch samples therefore have occurrence associations that indicate their contents in both the NEON Biorepository and main NEON data portals.  See NEON prototype dataset 9bc959c-148b-aaad-aa35-2d0805327428 available here.

Pre- and Post- Hurricane Maria Dry Soil Collection (GUAN, LAJA) (repackaging of occurrences published by the NEON Biorepository Data Portal)

These samples from Soil Collection (Distributed Periodic) (NEON-SOIC-DP) were collected at NEON GUAN and LAJA sites in Puerto Rico before (July and November 2017) and after (July 2018) Hurricane Maria, a Category 5 storm that affected the Carribean and occurred September 16, 2017 – October 2, 2017.

NEON Biorepository Aquatic Microalgae Collection (Chemical Preservation) (repackaging of occurrences published by the NEON Biorepository Data Portal)

This collection contains subsamples of aquatic microalgae preserved in either glutaraldehyde or a high-iodine Lugol's solution (NEON sample class: ptx_taxonomy_in.preserved). Periphyton and phytoplankton samples are collected three times per year at wadeable stream, river, and lake sites during aquatic biology bout windows, roughly in spring, summer, and fall. Benthic samples are collected using the most appropriate sampler for the habitat and substratum type, including rock scrubs, grab samples, and epiphyton. In wadeable streams, periphyton samples are collected in the two most dominant benthic habitat types (e.g. riffles, runs, pools, step pools), and seston samples were collected from the water column near the S2 sensor (seston samples were discontinued in 2018). In lakes, water-column phytoplankton samples are collected near the buoy and littoral sensors using a Kemmerer sampler, and in littoral areas using the best benthic sampling method for the dominant substratum type. In rivers, phytoplankton samples are collected near the buoy and two other deep-water locations using a Kemmerer or Van Dorn sampler, and in littoral areas using the best benthic sampling method for the dominant substratum type. All field-collected samples are split into subsamples in the domain support facility, preserved, and shipped to a contracting taxonomy laboratory where samples are further subsampled for analysis and archiving. All samples are archived in 20 mL glass scintillation vials and stored in a temperature (17-18°C) and humidity controlled environment. Phytoplankton and seston samples are preserved in a 2% high-iodine Lugol's solution from 2014-2020 and 0.5% glutaraldehyde starting in 2021. Periphyton samples are preserved in 0.5% glutaraldehyde. See related links below for protocols and NEON related data products.

NEON Biorepository Aquatic Microalgae Collection (Freeze-dried) (repackaging of occurrences published by the NEON Biorepository Data Portal)

This collection contains freeze-dried subsamples of aquatic microalgae (NEON sample class: ptx_taxonomy_in.freezeDried). Periphyton and phytoplankton samples are collected three times per year at wadeable stream, river, and lake sites during aquatic biology bout windows, roughly in spring, summer, and fall. Benthic samples are collected using the most appropriate sampler for the habitat and substratum type, including rock scrubs, grab samples, and epiphyton. In wadeable streams, periphyton samples are collected in the two most dominant benthic habitat types (e.g. riffles, runs, pools, step pools), and seston samples were collected from the water column near the S2 sensor (seston samples were discontinued in 2018). In lakes, water-column phytoplankton samples are collected near the buoy and littoral sensors using a Kemmerer sampler, and in littoral areas using the best benthic sampling method for the dominant substratum type. In rivers, phytoplankton samples are collected near the buoy and two other deep-water locations using a Kemmerer or Van Dorn sampler, and in littoral areas using the best benthic sampling method for the dominant substratum type. All field-collected samples are split into subsamples in the domain support facility, preserved, and shipped to a contracting taxonomy laboratory where samples are further subsampled for analysis and archiving. Freeze dried subsamples contained cleaned, freeze dried diatoms. Samples are archived in 20 mL glass scintillation vials and stored at room temperature. See related links below for protocols and NEON related data products.

NEON Biorepository Aquatic Microalgae Collection (Microscope Slides) (repackaging of occurrences published by the NEON Biorepository Data Portal)

This collection contains slide-mounted subsamples of aquatic microalgae (NEON sample class: ptx_taxonomy_in.slideID). Periphyton and phytoplankton samples are collected three times per year at wadeable stream, river, and lake sites during aquatic biology bout windows, roughly in spring, summer, and fall. Benthic samples are collected using the most appropriate sampler for the habitat and substratum type, including rock scrubs, grab samples, and epiphyton. In wadeable streams, periphyton samples are collected in the two most dominant benthic habitat types (e.g. riffles, runs, pools, step pools), and seston samples were collected from the water column near the S2 sensor (seston samples were discontinued in 2018). In lakes, water-column phytoplankton samples are collected near the buoy and littoral sensors using a Kemmerer sampler, and in littoral areas using the best benthic sampling method for the dominant substratum type. In rivers, phytoplankton samples are collected near the buoy and two other deep-water locations using a Kemmerer or Van Dorn sampler, and in littoral areas using the best benthic sampling method for the dominant substratum type. All field-collected samples are split into subsamples in the domain support facility, preserved, and shipped to a contracting taxonomy laboratory where samples are further subsampled for analysis and archiving. Algae specimens in this collection contain cleaned diatom subsamples that have been mounted on glass microscope slides and are archived at room temperature. See related links below for protocols and NEON related data products.

NEON Biorepository Benthic Microbe Collection (Sterivex Filters) (repackaging of occurrences published by the NEON Biorepository Data Portal)

This collection contains benthic biofilm samples collected on 67 mm long, 1.7 cm diameter, 0.22 um Sterivex capsule filters (NEON sample class: amb_fieldParent_in.archiveID). Benthic biofilm samples are collected 3 times per year at the same time and location as periphyton (microalgae) samples and microbe samples sent for sequencing analysis, three times per year in wadeable streams during aquatic biology bout windows, roughly in spring, summer, and fall. Benthic biofilms are not collected in lakes and rivers. Samples are collected from rock and wood scrubs using field-sterile methods, and filtered through a 0.22 um Sterivex SVGP capsule filters. In wadeable streams, periphyton samples are collected in the two most dominant benthic habitat types (e.g. riffles, runs, pools, step pools). Sterivex filters are capped and flash-frozen in the field and then shipped to the Biorepository to be archived at -80 degrees Celsius. See related links below for protocols and NEON related data products.

NEON HQ Soil Archive (Megapit) (repackaging of occurrences published by the NEON Biorepository Data Portal)

This collection contains soil samples collected from the megapit at each terrestrial site (NEON sample class: mgp_perarchivesample). During the construction of all 47 terrestrial field sites, "Megapit" soils were collected from multiple horizons at a single soil pit that was up to 2m deep. These samples serve as a reference of soil physical and chemical conditions at the time the NEON site was constructed. The Megapit Archive is curated at the NEON program headquarters in Boulder, CO. Megapit soil samples are available upon request (https://www.neonscience.org/samples/soil-archive).

NEON Biorepository Particulate Mass Filter Collection (repackaging of occurrences published by the NEON Biorepository Data Portal)

This collection contains quartz microfiber particulate mass filters (NEON sample class: dpm_fieldData_in.sampleID and dpm_filterBlank_in.sampleID). Particulate mass sampling is executed at six of NEON's terrestrial sites, located in Domains 10, 13, and 15. The subset of sites included for sampling are those in the Basin and Range, Eastern and Western slopes of the Rocky Mountains, and the Eastern plains of Colorado. This selection of sites enables focus on transportation of particulate matter from the Great Basin and the Colorado Plateau by prevailing westerly winds over the Colorado Rocky Mountains, to receptor sites in the Rockies and Great Plains. Samples are collected by an automated assembly that pulls air through a quartz microfiber filter with a porosity of 10 micrometers, to collect PM10. Filters are weighed at high precision pre- and post-deployment at the Colorado Department of Public Health and Environment Air Resources Laboratory to determine dust deposition mass. Filters are then shipped to the Biorepository to be archived at 4 degrees Celsius in air-tight plastic sleeves. Subsamples of the filters are available to the science community upon request to enable the assessment of chemical and nutrient inputs in the region. Additionally, 5 filter blanks from each box of filters are archived and available upon request. See related links below for protocols and NEON related data products.

NEON Biorepository Soil Collection (Distributed Periodic) (repackaging of occurrences published by the NEON Biorepository Data Portal)

This collection contains air-dried soil samples collected during periodic soil sampling at NEON terrestrial sites (NEON sample class: sls_bgcSubsampling_in.bgcArchiveID). Soil biogeochemical samples are collected once every 5 years, with three unique sampling locations per plot and ten plots per site. Soil sampling is conducted to a maximum depth of 30 ± 1 cm where possible. When organic (O) and mineral (M) horizons are present within a single profile they are separated prior to analysis and archiving. However, other sub-horizons are not separated. Soil from the O horizon is homogenized and non-soil material is removed by hand (no sieving), whereas soil from the M horizon is homogenized and sieved to 2 mm. Prior to archiving, all soil samples are air-dried, then placed into glass jars and stored at room temperature. See links below for NEON data products that provide various physical, chemical, and biological measurements (pH, moisture, carbon and nitrogen content and stable isotopes, inorganic nitrogen pools and net transformation rates, microbial community composition and biomass) for these same soils. In addition, a more detailed characterization of the dominant soil types at each site, including taxonomy, texture, bulk density, and geochemical properties, occurred during the construction period of NEON through two projects. These data are available in NEON data products Soil physical and chemical properties, distributed initial characterization (DP1.10047.001) and Soil physical and chemical properties, Megapit (DP1.00096.001).