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Fig. 3 in Diurnal accumulation of K -dependent L-asparaginase in leaf of common bean (Phaseolus vulgaris L.)

Fig. 3. Two-dimensional gel electrophoresis and immunoblotting of asparaginase in developing leaves. Arrows indicate the polypeptide precursor and α-subunit. The experimental pI of the α subunit was measured as 4.87.

Fig. 2 in Diurnal accumulation of K -dependent L-asparaginase in leaf of common bean (Phaseolus vulgaris L.)

Fig. 2. Diurnal accumulation of asparaginase and related metabolites in developing leaves. (A) Immunoblot and corresponding SDS-PAGE. The top band corresponds to the polypeptide precursor and lower band to the α-subunit. Position of molecular wt markers is indicated on the left; (B) asparaginase activity. Average ± s.d.; n = 3. (C) Concentration of asparagine (Asn), aspartate (Asp) and NH+ in leaf tissue. Average ± s.d.; n = 3.

Fig. 4 in Diurnal accumulation of K -dependent L-asparaginase in leaf of common bean (Phaseolus vulgaris L.)

Fig. 4. Analysis of circadian regulation of asparaginase protein. Plants were exposed to a 16 h light and 8 h dark cycle followed by exposure to continuous light. Leaves were sampled at the indicated times and asparaginase detected by immunoblotting.

Fig. 1 in Diurnal accumulation of K -dependent L-asparaginase in leaf of common bean (Phaseolus vulgaris L.)

Fig. 1. RNA-Seq expression profile of PvASPG1 and PvASPG2. Expression value is given as Z-score (Severin et al., 2010). Samples are grouped by tissue. Data are from O'Rourke et al. (2014) (see Supplementary Table S1).

Fig. 5 in Unique localization of jasmonic acid-related compounds in developing Phaseolus vulgaris L. (common bean) seeds revealed through desorption electrospray ionization-mass spectrometry imaging

Fig. 5. DESI-MS/MSI of OPDA and OPC-8 in the developing Phaseolus vulgaris seeds. (a) Optical image of the seed section for OPDA analysis. (b) MS/MS spectrum of precursor ion at m/z 291.1966 ± 1 Da obtained at the target enhanced mode for m/z 165.1. (c) Ion image at m/z 165.1300. (d) Optical image of the seed section for OPC-8:0 analysis. (e) MS/MS spectrum of precursor ion at m/z 293.2122 ± 1 Da obtained at the target enhanced mode for m/z 225.1. Ion images at m/z (f) 223.1400 and (g) 231.2142. Scale bar = 2 mm. Compound names are defined in Table 1.

Fig. 4 in Unique localization of jasmonic acid-related compounds in developing Phaseolus vulgaris L. (common bean) seeds revealed through desorption electrospray ionization-mass spectrometry imaging

Fig. 4. LC-ESI-MS/MS analysis of JA-related compounds in the extracts from the radicle and seed coat of developing Phaseolus vulgaris seeds. MS/MS spectra of peaks at (a) 5.3 min in Fig. 3c, (b) 5.3 min in Fig. 3d, (c) 6.5 min in Fig. 3c and (d) 6.5 min in Fig. 3d and (e) 6.3 min in Fig. 3e and (f) 6.3 min in Fig. 3f and (g) 6.4 min in Fig. 3e, (h) 6.4 min in Fig. 3f, (i) 6.7 min in Fig. 3e, and (j) 6.7 min in Fig. 3f. Compound names are defined in Table 1.

Fig. 2 in Unique localization of jasmonic acid-related compounds in developing Phaseolus vulgaris L. (common bean) seeds revealed through desorption electrospray ionization-mass spectrometry imaging

Fig. 2. LC-ESI-MS/MS analysis of JA-related compound standards. Spectra of (a) OPDA, (b) OPC-8:0, and (c) JA standards. Compound names are defined in Table 1.

Fig. 1 in Unique localization of jasmonic acid-related compounds in developing Phaseolus vulgaris L. (common bean) seeds revealed through desorption electrospray ionization-mass spectrometry imaging

Fig. 1. DESI-MSI analysis of JA-related compounds in the developing Phaseolus vulgaris seeds. (a) Optical image of the section. (b) Mass spectrum obtained from the section. Ion images of m/z (c) 277.2172, (d) 291.1953, and (e) 293.2117. Three different developing seeds were analyzed, and the results from one are shown as representative data. Scale bar = 2 mm. Compound names are defined in Table 1.

Fig. 3 in Unique localization of jasmonic acid-related compounds in developing Phaseolus vulgaris L. (common bean) seeds revealed through desorption electrospray ionization-mass spectrometry imaging

Fig. 3. LC-ESI-MS analysis of JA-related compounds in the extracts from the radicle and seed coat of developing Phaseolus vulgaris seeds. Base peak chromatogram of m/z 277.2173 ±10 ppm for (a) radicle and (b) seed coat, m/z 291.1966 ± 10 ppm for (c) radicle and (d) seed coat, and m/z 293.2122 ± 10 ppm for (e) radicle and seed coat, respectively. Peaks with arrow indicates JA-related compounds: (a) and (b) αLA, (c) and (d) OPDA, and (e) and (f) OPC-8:0. Compound names are defined in Table 1.