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Figure 1 from: Tuf IH, Drábková L, Šipoš J (2015) Personality affects defensive behaviour of Porcellio scaber (Isopoda, Oniscidea). In: Taiti S, Hornung E, Štrus J, Bouchon D (Eds) Trends in Terrestrial Isopod Biology. ZooKeys 515: 159–171. https://doi.org/10.3897/zookeys.515.9429

Figure 1 - Design of one experimental set. Dashed arrows symbolise repeated stimuli applied if previous stimulus did not evoke tonic immobility. Experimental sets were applied repeatedly over a three week period; each individual was exposed to five experimental sets with 4 days intervals between.

opencc-by-4.0Jul 2015View details →
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Figure 3 from: Tuf IH, Drábková L, Šipoš J (2015) Personality affects defensive behaviour of Porcellio scaber (Isopoda, Oniscidea). In: Taiti S, Hornung E, Štrus J, Bouchon D (Eds) Trends in Terrestrial Isopod Biology. ZooKeys 515: 159–171. https://doi.org/10.3897/zookeys.515.9429

Figure 3 - Endurance of tonic immobility of Porcellio scaber of different body sizes induced by treatments. (*** p < 0.001; ** p < 0.01; * p ≤ 0.05)

opencc-by-4.0Jul 2015View details →
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Supplementary material 5 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

SI Figure 5. Boxplots depicting individual measurements of basal membrane labyrinth depth :

opencc-zeroDec 2018View details →
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Supplementary material 3 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

SI Figure 3. Boxplots depicting individual measurements of basal lamina thickness :

opencc-zeroDec 2018View details →
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Supplementary material 2 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

SI Figure 2. Boxplots depicting individual measurements of cuticle thickness :

opencc-zeroDec 2018View details →
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Supplementary material 4 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

SI Figure 4. Boxplots depicting individual measurements of apical membrane labyrinth depth :

opencc-zeroDec 2018View details →
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Supplementary material 1 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

SI Figure 1. Boxplots depicting individual measurements of cell size and cell nuclei diameter :

opencc-zeroDec 2018View details →
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Figure 8 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

Figure 8 Ultrastructure of the apical plasma membrane labyrinth in the anterior chamber and the papillate region. A Apical membrane labyrinth (AL) in the anterior chamber. In the cytoplasmic strands mitochondria (arrowheads) are present B A tubular appearance of cytoplasmic strands is evident in certain sections (arrow) C Apical membrane labyrinth (AL) in the papillate region. Key: arrowheads – mitochondria, CUT – cuticle.

opencc-by-4.0Dec 2018View details →
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Figure 5 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

Figure 5 Cuticle and basal lamina thickness in the two hindgut regions. Median values of cuticle thickness (A) and basal lamina thickness (B) in cells from the anterior chamber (blue) and the papillate region (red) of three specimens.

opencc-by-4.0Dec 2018View details →
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Figure 4 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

Figure 4 Ultrastructure of the cuticle in the anterior chamber and the papillate region. A The cuticle in the anterior chamber consists of thin epicuticle (EPI) and thick procuticle (PRO) with distinct sublayers. Between the epicuticule and the procuticle, a thin layer of intermediate electron density is present (arrow) B Cuticle in the papillate region consists of epicuticle (EPI) which is proportionally thicker according to the procuticle (PRO). Procuticle sublayers are less pronounced than in the anterior chamber. Between the epicuticle and the procuticle a thin layer of intermediate electron density is present (arrow) C, D The outer part of the epicuticle (EPI) in the anterior chamber (C) and in the papillate region (D) is three-layered (arrow) and covered with a fuzzy layer (arrowhead) E Procuticle (PRO) in the papillate region can be of approximately the same thickness as epicuticle (EPI) and without apparent sublayers.

opencc-by-4.0Dec 2018View details →
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Figure 2 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

Figure 2 Histological structure of the hindgut epithelium in the anterior chamber and papillate region. A Ventral-lateral epithelium in the anterior chamber B Apical parts of ventral and lateral epithelial cells are bulging into the hindgut lumen C Dorsal epithelium in the anterior chamber forms typhlosole (T) and two typhlosole channels (TC) D Epithelial cell of typhlosole E Epithelial cell of typhlosole channel F Hindgut epithelium in the papillate region G Basal parts of epithelial cells in the papillate region are bulging into haemocoel. Abbreviations: M – muscles, N – cell nucleus.

opencc-by-4.0Dec 2018View details →
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Figure 13 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

Figure 13 Localization of Na+/K+-ATPase activity in hindguts of intermoult and postmoult animals. A Na+/K+-ATPase activity in the anterior chamber of intermoult P.scaber. Electron dense deposits are present along tubular (arrowheads) and dilated (arrows) apical membranous infoldings and in the procuticle (PRO) B Na+/K+-ATPase activity in the papillate region of postmoult P.scaber. Abundant deposits are present along the apical infoldings (arrowheads) and in the procuticle (PRO) C Control section in the anterior chamber of intermoult animal where K+ ions were replaced by Na+ ions. No reaction product is present along apical infoldings (AI) or in the cuticle (C) D Control section in the papillate region of postmoult animal treated with ouabain. Some deposits (arrowheads) are present along the apical infoldings. Abbreviations: C – cuticle.

opencc-by-4.0Dec 2018View details →
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Figure 11 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

Figure 11 Ultrastructure of cell junctions in the anterior chamber and the papillate region. A The junctional complex in the anterior chamber consists of subapically located adherens junctions (AJ) and septate junctions (SJ) located beneath the adherens junctions B The junctional complex in the papillate region consists of subapically located adherens junctions (AJ) and extremely long and convoluted septate junctions (SJ) located beneath the adherens junctions C Adherens junctions consist of two electron dense plaques (arrowheads) at the cytoplasmic sides of lateral plasma membranes of two neighbouring cells and electron dense material in the intercellular space between the membranes D Septate junctions are visible as electron dense septa arranged in strings (arrow). Dilated intercellular spaces are visible where septate junctions are locally interrupted (arrowhead). Abbreviations: C – cuticle, AI – apical infoldings, SJ – septate junction.

opencc-by-4.0Dec 2018View details →
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Figure 1 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

Figure 1 Measurements in ImageJ/Fiji. A Measurements of cell width (line a), height (line b) and nucleus diameter (line c) B Measurements of cuticle and basal lamina thickness. Cuticle and basal lamina thickness (yellow lines) were measured at intersections of grid lines (blue grid) with cuticle/basal lamina surface C Measurements of membrane labyrinths depth. Membrane labyrinth depths (yellow lines) were measured at intersections of grid lines (blue grid) with the outline of membrane labyrinth edge (red line) D Measurements of spatial density of membrane infoldings. The length of apical/basal surface outline (red line) was measured and the infoldings along the outline (yellow points) were counted.

opencc-by-4.0Dec 2018View details →
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Figure 9 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

Figure 9 Ultrastructure of the basal plasma membrane labyrinth in the anterior chamber and the papillate region. A Basal plasma membrane in the anterior chamber forms sparse narrow infoldings (arrows) B Extensive basal membrane labyrinth (BL) in the papillate region is associated with numerous mitochondria (arrowheads) C Basal lamina in the anterior chamber. Hemidesmosome-like junctions are visible as small electron dense plaques (arrowheads) D Basal lamina in the papillate region. Hemidesmosome-like junctions are visible as small electron dense plaques (arrowheads). Abbreviation: M – muscle.

opencc-by-4.0Dec 2018View details →
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Figure 12 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

Figure 12 Lateral parts of epithelial cells in the anterior chamber and the papillate region. A Two neighbouring cells in the anterior chamber B Two neighbouring cells in the papillate region. Abbreviations: LID – area of lateral interdigitations, AL – apical labyrinth, BL – basal labyrinth, M – muscle.

opencc-by-4.0Dec 2018View details →
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Figure 7 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

Figure 7 Bacteria associated with hindgut cuticle in the papillate region of one specimen. A bacteria are visible near or at the cuticle (C) surface. Bacterial cells are connected to each other with thin filamentous structures (arrow) B bacteria are connected to the fuzzy layer of epicuticle (arrow) by thin filamentous structures (arrowheads).

opencc-by-4.0Dec 2018View details →
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Figure 10 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

Figure 10 Apical and basal labyrinth depth and density of membrane infoldings in the two hindgut regions. Scatter plots depict: A Median values of apical labyrinth depth versus the median values of basal labyrinth depth in cells from the anterior chamber (blue) and the papillate region (red) of three specimens B Median values of apical infoldings density against the median values of basal infoldings density in cells from the anterior chamber (blue) and the papillate region (red) of three specimens. The central line in both plots is the line of equality. Points lying on the line indicate that median value of depth/density is equal, apically and basally. Points below the line indicate that the median value of depth/density is larger apically than basally. Points above the line indicate that the median value is larger basally than apically.

opencc-by-4.0Dec 2018View details →
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Figure 3 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

Figure 3 Cell width, cell height and cell nucleus diameter in the two hindgut regions. Scatter plot depicting median values of cell width, cell height and cell nucleus diameter in the anterior chamber against median values of same parameters in the papillate region of seven specimens. The central line is the line of equality. Points lying on the line indicate that the median value of measured parameter is equal in both hindgut regions. Points below the line indicate that the median value of measured parameter is larger in the anterior chamber than in the papillate region. Points above the line indicate that the median value of measured parameter is larger in the papillate region than in the anterior chamber.

opencc-by-4.0Dec 2018View details →
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Figure 6 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395

Figure 6 Ultrastructure of junctions between cuticle and apical plasma membrane. A Junctions between cuticle and apical plasma membrane in the anterior chamber visible as electron dense structures (arrows), from which fibers (arrowheads) extend into the procuticle B Junctions between cuticle and apical plasma membrane in the papillate region (arrowheads) associated with abundant bundles of microtubules inside the cell (arrow) C, D Higher resolution images of bundles of microtubules (MTB) associated with junctions between cuticle and apical plasma membrane. Image D displays the area denoted on image C (red rectangle) where individual microtubules (arrows) can be discerned.

opencc-by-4.0Dec 2018View details →

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