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45 results for “RFLP”
Figure 1 in Investigation of GH and GHR Alu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method
Figure 1. PCR products of exons 4 and 5 of the GH gene (428-bp band, M: 100-bp DNA ladder).
FIGURE 4 in Description of a new Fridericia species (Oligochaeta: Enchytraeidae) and its molecular comparison with two morphologically similar species by PCRRFLP
FIGURE 4. Fridericia crassiductata sp.n. Fridericia crassiductata sp.n. A = praeclitellar segments (½ X – ½ XII) (sperm funnels marked with arrows, B = Photograph of bursal slits (marked with arrows) of the male copulatory organs, C = Photograph of the subneural gland in XIV (marked with arrow).
FIGURE 3 in Description of a new Fridericia species (Oligochaeta: Enchytraeidae) and its molecular comparison with two morphologically similar species by PCRRFLP
FIGURE 3. Fridericia crassiductata sp.n. Fridericia crassiductata sp.n. Photograph of the clitellar glands (dorsal view).
FIGURE 1 in Description of a new Fridericia species (Oligochaeta: Enchytraeidae) and its molecular comparison with two morphologically similar species by PCRRFLP
FIGURE 1. Fridericia crassiductata sp.n. A = setal bundles of a praeclitellar segment (lateroventral view), B = Photograph of the cutaneous glands of a praeclitellar segment (dorsal view), C = Photograph of the head (head pore marked with arrows), D = Photograph of the head (brain marked with arrows), E = Photograph of the coelomocytes. Each photograph is sized with a black line representing 50 μm length.
FIGURE 6 in Description of a new Fridericia species (Oligochaeta: Enchytraeidae) and its molecular comparison with two morphologically similar species by PCRRFLP
FIGURE 6. The gel image BsuRI of restriction digestion. The numbering of samples corresponds with the number in Table 1. The character M nominates the molecular marker, the length of fragments are represented on the left side of the photo (also by Fig. 7–10).
FIGURE 5 in Description of a new Fridericia species (Oligochaeta: Enchytraeidae) and its molecular comparison with two morphologically similar species by PCRRFLP
FIGURE 5. Fridericia crassiductata sp.n. A = Photograph of the spermatheca. (the diverticle fill with sperm around the ampulla marked with black arrows, the ectal duct with white arrows, ectal gland with asterisk, B = Photograph of the opening of spermathecal ectal duct (glands marked with black arrows, duct with white arrow), C = Photograph of the opening of spermathecal ectal duct three glands marked with black arrows, duct with white arrow).
FIGURE 4. Distinctive RFLP pattern obtained with pDRAW32 in Revision of the genus Reddellomyces (Tuberaceae): a combination of molecular and morphological analysis provides insights into species diversity
FIGURE 4. Distinctive RFLP pattern obtained with pDRAW32 from in silico digestion of ITS rDNA sequences from representative species. In the computer-simulated digestions, the set of seven enzymes AoxI, BtsCI, FatI, GlaI, HaeIII, Hpy1881, HpyCH4V, LmnI, NIaIII, PasI, StyI and TaqI were used. Lanes labelled MW represent Invitrogen 100 kb ladder.
Data from: Development of a PCR-RFLP assay to identify Drosophila melanogaster among field-collected larvae
The fruit fly Drosophila melanogaster is a model organism to study several aspects of metazoan biology. Most of the work has been conducted in adult fruit flies, including laboratory and field-derived specimens, but Drosophila melanogaster larvae recently became a valuable model to better understand animal physiology, development or host-microbe interactions. While adult flies can be easily assigned to a given Drosophila species based on morphological characteristics, such visual identification is more intricate at the larval stage. This could explain the limited number of studies focusing on larvae, especially field-derived samples. Here, we developed a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) assay that discriminates D. melanogaster from other ecologically relevant Drosophila species at the larval stage. The method, which targets the cytochrome oxidase I (COI) gene, was validated using laboratory-derived larvae from seven D. melanogaster populations originating from different geographic areas as well as six Drosophila species. We further validated this PCR-RFLP assay in a natural context, by identifying wild larvae collected in two locations in France. Notably, among all PCR-RFLP profiles that matched the D. melanogaster species, 100% were correctly identified, as confirmed by COI sequencing. In summary, our work provides a rapid, simple and accurate molecular tool to identify D. melanogaster from field-collected larvae.
FIGURE 2 in Identification of Planococcus ficus and Planococcus citri (Hemiptera: Pseudococcidae) by PCR-RFLP of COI gene
FIGURE 2. RFLP analysis with HinfI restriction enzyme of COI gene. Digested products were separated on 2% agarose gels stained with ethidium bromide. Lanes: 1-8, Pl citri; 9-13, Pl ficus; M, 100 bp ladder.
FIGURE 1 in Identification of Planococcus ficus and Planococcus citri (Hemiptera: Pseudococcidae) by PCR-RFLP of COI gene
FIGURE 1. COI gene restriction patterns of Ps. longispinus (L), Pl. citri (C) and Pl. ficus (F) generated by BspPI, HinfI and SspI.
Figure 1 in Genetic diversity of Atherina hepsetus (Osteichthyes: Atherinidae) populations as determined by RFLP analysis of three mtDNA regions
Figure 1. Sampling sites: Monastiraki (MON), Panagopoula (PAN), Kiparissi (KIP), Tinos (TIN), Naxos (NAX), Samos (SAM), Nissiros (NIS), Leipsi (LEI), Kos (KOS), Lesvos (MYT) and Evvoia (EVV).
Figure 3 in Genetic diversity of Atherina hepsetus (Osteichthyes: Atherinidae) populations as determined by RFLP analysis of three mtDNA regions
Figure 3. Dollo parsimony (Farris 1977) dendrogram showing the relationships between the 15 mtDNA haplotypes detected. Numbers indicate the bootstrap support (10,000 replicates) of each node of the majority-rule consensus tree.
Data from: Development of a PCR-RFLP assay to identify Drosophila melanogaster among field-collected larvae
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FIGURE 7 in Description of a new Fridericia species (Oligochaeta: Enchytraeidae) and its molecular comparison with two morphologically similar species by PCRRFLP
FIGURE 7. The gel image TaqI of restriction digestion.
FIGURE 2 in Description of a new Fridericia species (Oligochaeta: Enchytraeidae) and its molecular comparison with two morphologically similar species by PCRRFLP
FIGURE 2. Fridericia crassiductata sp.n. A = oesophageal appendage, B = spermatheca.
FIGURE 9. The gel image Hin6I in Description of a new Fridericia species (Oligochaeta: Enchytraeidae) and its molecular comparison with two morphologically similar species by PCRRFLP
FIGURE 9. The gel image Hin6I of restriction digestion.
FIGURE 8 in Description of a new Fridericia species (Oligochaeta: Enchytraeidae) and its molecular comparison with two morphologically similar species by PCRRFLP
FIGURE 8. The gel image AluI of restriction digestion.
FIGURE 10. The gel image Csp6I in Description of a new Fridericia species (Oligochaeta: Enchytraeidae) and its molecular comparison with two morphologically similar species by PCRRFLP
FIGURE 10. The gel image Csp6I of restriction digestion.
Figure 2 in Genetic diversity of Atherina hepsetus (Osteichthyes: Atherinidae) populations as determined by RFLP analysis of three mtDNA regions
Figure 2. Neighbor-joining (Saitou and Nei 1987) cladogram, based on the net nucleotide divergence.
Sex identification PCR-RFLP assay tested in eight species of Sebastes rockfish
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