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45 results for “RFLP”
Soil C, N, P, and Frankia nifD-K RFLP genotypes distribution from Alnus tenuifolia nodules in early and late succession 2005
This dataset contains genetic characterizations of Frankia inhabiting Alnus tenuifolia nodules in early and late succession sites in the Bonanza Creek Experimental Forest (BCEF). Characterizations were done using PCR-RFLP on the nifD-K spacer region of the Frankia genome. The position of each plant and each nodule were mapped in order to examine spatial patterns in Frankia distribution within sites. Soil chemistry data, including C, N, P, and pH, were also collected from mineral and organic layers.
Figure 4 in PCR-RFLP Based genetic diversity of Plasmodium vivax genotypes in district Mardan, Pakistan
Figure 4. Prevalence of six different sub-allele types of Pvmsp-3β (A1-A3, B1-B2 and C1) based on PCR-RFLP.
Figure 2 in PCR-RFLP Based genetic diversity of Plasmodium vivax genotypes in district Mardan, Pakistan
Figure 2. Prevalence of nine different sub-allele types of Pvmsp- 3α(A1-A4), (B1-B3), C1 and D are the nine different alleles from PCR-RFLP.
Figure 3 in Revised classification design of the Anatolian species of Nannospalax (Rodentia: Spalacidae) using RFLP analysis
Figure 3. Neighbor-joining and span tree showing genetic relationships among populations, based on Nei's genetic distance measure.
Figure 1 in Revised classification design of the Anatolian species of Nannospalax (Rodentia: Spalacidae) using RFLP analysis
Figure 1. Sampling localities of Nannospalax xanthodon and Nannospalax ehrenbergi from Turkey for molecular studies. Names for numbered localities indicated in Table 1.
Figure 3 in Determination of genetic variations between Apodemus mystacinus populations distributed in Turkey inferred from mtDNA PCR-RFLP
Figure 3. Restriction patterns of HinfI inferred from D-loop digestion (M: Marker–100bp DNA Ladder, 1. Ordu, 2. Trabzon, 3. Rize, 4. Artvin, 5–6. Erzincan, 7–8. Kahramanmaraş, 9. Adıyaman, 10–11. Adana, 12. Muğla, 13. Burdur, 14. Konya, 15. Antalya, 16. Mersin, 17. Kastamonu, 18. Zonguldak, 19. Düzce, 20. Balıkesir, 21. İzmir, 22. Aydın, 23. A. uralensis, 24. A. witherbyi, 25. D-loop PCR products).
Figure 2 in Determination of genetic variations between Apodemus mystacinus populations distributed in Turkey inferred from mtDNA PCR-RFLP
Figure 2. Restriction patterns of MboI, HaeIII, and RsaI inferred from cytb digestion (M: Marker–100bp DNA Ladder, 1. Ordu, 2. Trabzon, 3. Rize, 4. Artvin, 5. Erzincan, 6. Kahramanmaraş, 7. Adıyaman, 8. Adana, 9. Muğla, 10. Burdur, 11. Konya, 12. Antalya, 13. Mersin, 14. Kastamonu, 15. Zonguldak, 16. Düzce, 17. Balıkesir, 18. İzmir, 19. Aydın, 20. A. uralensis, 21. A. witherbyi, 22. Cytb PCR product).
Figure 5 in Determination of genetic variations between Apodemus mystacinus populations distributed in Turkey inferred from mtDNA PCR-RFLP
Figure 5. PCoA analysis of A. mystacinus clades. The scatter plot is of the scores of three principal eigenvalues inferred from NTSYS software. Each scatter point represents a specimen of A. mystacinus.
Figure 1 in Determination of genetic variations between Apodemus mystacinus populations distributed in Turkey inferred from mtDNA PCR-RFLP
Figure 1. Sampling localities of A. mystacinus specimens. Table 2. Restriction enzymes and their digestion sites with reaction procedures.
Figure 2 in Investigation of GH and GHR Alu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method
Figure 2. Enzyme digestion results of exons 4 and 5 of the GH gene (M: 50-bp DNA ladder; 1–5 and 7: LL genotype, 6: LV genotype).
Figure 4 in Investigation of GH and GHR Alu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method
Figure 4. Enzyme digestion results of the exon 10 region of the GHR gene (M: 50-bp DNA ladder, A: AG genotype, B: AA genotype).
Fig. 1 in Anisakid nematode species identification in harbour porpoises (Phocoena phocoena) from the North Sea, Baltic Sea and North Atlantic using RFLP analysis
Fig. 1. RFLP profiles obtained by digestion of ITS1-5.8S-ITS2 region with the restriction enzymes HinfI, RsaI and HaeIII. a)-i) lane 1–5: Anisakid nematodes from harbour porpoises. j)-l) lane 1–3: A. simplex s. s. from North Sea, Baltic and Norwegian harbour porpoises; lane 4–6: P. decipiens s. s. from North Sea and Baltic harbour and grey seals; lane 7–9: C. osculatum s. s. from North Sea and Baltic harbour and grey seals. L: 100-bp ladder.
Fig. 2. The PCR products identified within the 18S in Usefulness of PCR-RFLP of 18S rRNA gene for rapid post-mortem diagnostics of highly pathogenic Eimeria spp. (Apicomplexa: Eimeriidae) of European bison, Bison bonasus L. with histopathological correlation
Fig. 2. The PCR products identified within the 18S rRNA of Eimeria bovis following digestion with two restriction endonucleases: AluI recognising AG∧CT and Hin1II recognising CATG∧. M1: GeneRuler 100 bp Plus DNA Ladder (Thermo Fisher Scientific); M2: GeneRuler 50bp DNA Ladder (Thermo Fisher Scientific); lane 1: European bison colon wall tissue; lane 2: European bison colon wall tissue after digestion; lane 3: E. bovis oocysts of European bison; lane 4: E. bovis oocysts of European bison after digestion.
Fig. 3 in Usefulness of PCR-RFLP of 18S rRNA gene for rapid post-mortem diagnostics of highly pathogenic Eimeria spp. (Apicomplexa: Eimeriidae) of European bison, Bison bonasus L. with histopathological correlation
Fig. 3. The virtual double digestion of the 18S rRNA gene of eimerians infecting the large intestine of the European bison with the restriction enzymes Mval (BstNI) recognising CC∧WGG, and KpnI recognising GGTAC∧C, simulated with SnapGene version 5.0.6 (GSL Biotech LLC); M: GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific). (A) A three-band pattern for E. bovis (20 bp, 210 bp, 343 bp). (B) A four-band pattern for E, zuernii (20 bp, 100 bp, 210 bp, 242 bp). (C) A two-band pattern for E. alabamensis (212 bp, 362 bp).
Fig. 1 in Usefulness of PCR-RFLP of 18S rRNA gene for rapid post-mortem diagnostics of highly pathogenic Eimeria spp. (Apicomplexa: Eimeriidae) of European bison, Bison bonasus L. with histopathological correlation
Fig. 1. Histopathological lesions associated with endogenous stages of Eimeria spp. in sections of the ileum and colon of European bison (H-E staining). (A) Shortening and blunting of the intestinal villi of the ileum with diffuse infiltration of mononuclear inflammatory cells within the lamina propria, edematous stroma, dilated crypt containing necrotic debris (arrow), and atrophy of submucosal lymphoid follicles (× 20 magnification). (B) Schizonts and degenerating merozoites in the crypt lumen of the colon (arrows); immature macrogamont with a central nucleus (arrowhead) (× 1000 magnification). (C) Immature microgamonts in the epithelial cells of the colon crypt (arrows) (× 400 magnification). (D) Mature microgamont in the epithelial cells of the colon crypt (arrow) (× 1000 magnification). (E) Gametogonic stages of Eimeria development in the epithelial cells of the colon. Microgamont with peripheral microgames (arrowhead), (a) nearly mature microgamonts, (b) macrogamont with eosinophilic wall-forming bodies, (c) early oocyst (× 400 magnification). (F) Mature macrogamont in the epithelial cells of the cecum (arrow) (× 1000 magnification).
Fig. 6. Electrophoresis agarose gel showing RFLP pattern comparison between the F2 in Detection of maize bushy stunt phytoplasma in leafoppers collected in native corn crops grown at high elevations in southeast Mexico
Fig. 6. Electrophoresis agarose gel showing RFLP pattern comparison between the F2nR2 sequences amplified from Dalbulus elimatus and Idiodonus wickhami digested with AluI, BstUI, HaeIII, HinfI, and Tsp509I. Molecular weight (MW) marker, 1 kb plus.
Figure 1 in PCR-RFLP Based genetic diversity of Plasmodium vivax genotypes in district Mardan, Pakistan
Figure 1. Prevalence of four different alleles of Pvmsp-3α (A, B, C and D) from PCR-RFLP
Figure 3 in PCR-RFLP Based genetic diversity of Plasmodium vivax genotypes in district Mardan, Pakistan
Figure 3. Prevalence of three different alleles of Pvmsp-3β (A, B, C) from PCR-RFLP.
Figure 2 in Revised classification design of the Anatolian species of Nannospalax (Rodentia: Spalacidae) using RFLP analysis
Figure 2. Principal coordinate analysis plot of the chromosomal races of Nannospalax.
Figure 4 in Determination of genetic variations between Apodemus mystacinus populations distributed in Turkey inferred from mtDNA PCR-RFLP
Figure 4. UPGMA dendrogram of the composite data by combining cytb and D-loop regions.
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