Find research datasets worth reusing

opencc-by-4.0Nov 2020View details →

Meta-Analysis on the Effect of Biopreservatives on Staphylococcus aureus in Cheese

Recording of the talk “Meta-analysis on the effect of biopreservatives on Staphylococcus aureus in cheese” presented by Ursula Gonzales-Barron at the 71st Annual Meeting of the European Federation of Animal Science, EAAP, Online virtual meeting (1-4 Dec 2020).

opencc-by-4.0Oct 2020View details →

Meta-regression Models Describing the Effects of Essential Oils and Added Lactic Acid Bacteria on Staphylococcus aureus Inactivation in Cheese

Recording of the talk “Meta-regression models describing the effects of essential oils and added lactic acid bacteria on Staphylococcus aureus inactivation in cheese”, presented by Beatriz Nunes Silva at the 2020 International Association for Food Protection Annual Meeting, IAFP, Online virtual meeting (26-28 Oct 2020).

opencc-by-4.0Dec 2022View details →

Figure 1 in Metal nanoparticles produced by plants with antibacterial properties against Staphylococcus aureus

Figure 1. Synthesis of metal nanoparticles using plant extracts.

opencc-by-4.0Jul 2019View details →

Supervised Molecular Dynamics Movies from: Deciphering the molecular recognition mechanism of multidrug resistance Staphylococcus aureus NorA efflux pump using a Supervised Molecular Dynamics approach

Molecular Recognition pathway of Supervised molecular dynamic simulations of MdfA-CLM NorA-CPX and  NorA-CPX.

opencc-by-4.0Aug 2019View details →

Deciphering the molecular recognition mechanism of multidrug resistance Staphylococcus aureus NorA efflux pump using a Supervised Molecular Dynamics approach.

Legend of Movie-S1 The Movie is composed by four synchronized and animated panels that show different aspects of the SuMD simulation. The time evolution is reported in nanosecond. In the first panel (upper left), the molecular representation of the system is shown. The MdfA backbone is represented by the new cartoon style (cyan). The CLM is shown in yellow and by a transparent surface. The protein residues within 3 Å from the ligand are made explicit by a stick representation. In the second panel (upper-right), the CM-distance between the protein and the ligand centers of mass is reported. In the third panel (lower left), the MMGBSA energy profile is reported. In the fourth panel (lower-right) cumulative electrostatic interactions are reported for the 15 MdfA residues most contacted by CLM during the whole simulation.   Legend of Video-S2 The Movie shows the SuMD trajectory of CLM on MdfA compared to the CLM crystallographic pose. MdfA is represented in cyan new cartoon transparency. The crystallographic pose is showed in yellow while the experimental one in light green. At 16.69 ns a RMSD value of 1.77 Å is highlighted.   Legend of Video-S3 The Movie is composed by four synchronized and animated panels that show different aspects of the SuMD simulation. The time evolution is reported in nanosecond. In the first panel (upper left), the system is shown. The NorA backbone is represented by the new cartoon style (red) and the protein residues within 3 Å of CPX are showed in stick. CPX is rendered by a green stick. In the second panel (upper-right), the distance between the centre of mass of the ligand and the protein during the trajectory is reported. In the third panel (lower left), the MMGBSA energy profile is reported. In the fourth panel (lower-right) cumulative electrostatic interactions are reported for the 15 NorA residues most contacted by CPX during the whole SuMD trajectory. It is important to note that the following video has a duration that is half of the simulation of SuMD. However, this straid does not alter the description of the trajectory performed by the ligand.   Legend of Video-S4 The Movie depicts the clustering analysis of CPX during the whole SuMD simulation. The NorA protein is shown in red new cartoon transparency. CPX is rendered by a light-green stick and by a transparent surface. The spheres are shown in 7 different colours, according to the different clusters. Each sphere dimension is in according to the cluster dimensions. After a first recognition site, the ligand conformations are clustered in different sites of the NorA channel. It is important to note that the following video has a duration that is half of the simulation of SuMD. However, this straid does not alter the description of the trajectory performed by the ligand.

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Disturbing the spatial structure of biofilms affects the expression of agr regulated virulence factors in Staphylococcus aureus

Staphylococcus aureus uses quorum sensing and nutrient availability to control the expression of agr-regulated virulence factors. Quorum sensing is mediated by autoinducing peptide (AIP), which at high concentration, reduces expression of surface attachment proteins (coa, fnbpA), and increases expression of exotoxins (lukS) and proteases (splA). Nutrient availability can be sensed through the saeS/saeR system. Low nutrients increase expression of saeR, which augments expression of coa and fnbpA distinct from AIP. The formation of spatial structure, such as biofilms, can alter quorum sensing and nutrient acquisition. In natural environments, biofilms encounter forces that may alter their spatial structure. This may impact quorum sensing and/or nutrient acquisition, and thus affect the expression of agr-regulated virulence factors. However, this has not been studied. We show that periodically disturbing biofilms composed of S. aureus using a physical force affects the expression of agr-regulated virulence factors. In nutrient-poor environments, disturbance increased the expression of coa, fnbpA, lukS, and splA. Disturbance into a nutrient-rich environment at low or high disturbance amplitudes moderately reduced expression of coa and fnbpA but increased expression of lukS and splA. Interestingly, at an intermediate amplitude, the overall expression of agr-regulated virulence factors was the lowest; expression of lukS and splA remained unchanged relative to an undisturbed biofilm while expression of coa and fnbpA significantly decreased. We hypothesize that these changes are a result of disturbance-driven changes in access to AIP and nutrients. Our results may allow the identification of environments where virulence is enhanced, or reduced, owing to disturbance.

opencc-zeroJan 2023View details →

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Data for: Copper ions inhibit pentose phosphate pathway function in Staphylococcus aureus

To gain a better insight of how Cu ions toxify cells, metabolomic analyses were performed in S. aureus strains that lack the described Cu ion detoxification systems (ΔcopBL ΔcopAZ; cop-). Exposure of the cop- strain to Cu(II) resulted in an increase in the concentrations of metabolites utilized to synthesize phosphoribosyl diphosphate (PRPP). PRPP is created using the enzyme phosphoribosylpyrophosphate synthetase (Prs) which catalyzes the interconversion of ATP and ribose 5-phosphate to PRPP and AMP. Supplementing growth medium with metabolites requiring PRPP for synthesis improved growth in the presence of Cu(II). A suppressor screen revealed that a strain with a lesion in the gene coding adenine phosphoribosyltransferase (apt) was more resistant to Cu. Apt catalyzes the conversion of adenine with PRPP to AMP. The apt mutant had an increased pool of adenine suggesting that the PRPP pool was being redirected. Over-production of apt, or alternate enzymes that utilize PRPP, increased sensitivity to Cu(II). Increasing or decreasing expression of prs resulted in decreased and increased sensitivity to growth in the presence of Cu(II), respectively. We demonstrate that Prs is inhibited by Cu ions in vivo and in vitro and that treatment of cells with Cu(II) results in decreased PRPP levels. Lastly, we establish that S. aureus that lacks the ability to remove Cu ions from the cytosol is defective in colonizing the airway in a murine model of acute pneumonia, as well as the skin. The data presented are consistent with a model wherein Cu ions inhibits pentose phosphate pathway function and are used by the immune system to prevent S. aureus infections.

opencc-zeroMay 2023View details →

opencc-by-4.0Aug 2023View details →

Figure 8: Theoretical Prediction of Oxacillin Resistance in Staphylococcus Aureus 2020-2030

Figure 8: Theoretical Prediction of Oxacillin Resistance in Staphylococcus Aureus 2020-2030

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Cystic fibrosis autoantibody signatures associate with Staphylococcus aureus lung infection or cystic fibrosis-related diabetes

While cystic fibrosis (CF) lung disease is characterized by persistent inflammation and infections, and chronic inflammatory diseases are often accompanied by autoimmunity, autooimmune reactivity in CF has not been studied in depth. In this work, we undertook an unbiased approach to explore the systemic autoantibody repertoire in CF. Our results show higher levels of several new autoantibodies in the blood of people with CF (PwCF) compared to control subjects. Some of these are IgA autoantibodies targeting neutrophil components or autoantigens linked to neutrophil-mediated tissue damage in CF. We also found that PwCF with higher systemic IgM autoantibody levels have lower prevalence of S. aureus infection. On the other hand, IgM autoantibody levels in S. aureus-infected PwCF correlate with lung disease severity. Diabetic PwCF have significantly higher levels of IgA autoantibodies in their circulation compared to nondiabetic PwCF, and several of their IgM autoantibodies are associated with worse lung disease. In contrast, in nondiabetic PwCF blood levels of IgA autoantibodies correlate with lung disease. We have also identified other autoantibodies in CF that are associated with P. aeruginosa airway infection. In summary, we have identified several new autoantibodies and associations of autoantibody signatures with specific clinical features in CF.

opencc-zeroAug 2023View details →

opencc-by-4.0Sep 2023View details →

Data for A Far-Red Fluorescent Probe to Visualize Staphylococcus aureus in Patient Samples

Raw and processed data supporting the manuscript "A Far-Red Fluorescent Probe to Visualize Staphylococcus aureus in Patient Samples"

opencc-by-4.0Dec 2022View details →

Airway environment drives the selection of quorum sensing mutants and promote Staphylococcus aureus chronic lifestyle

Sequence data linked to the manuscript "Airway environment drives the selection of quorum sensing mutants and promote Staphylococcus aureus chronic lifestyle" - Shotgun metagenomic from sputa of Patients A B C H J - 10 hemolytic and 10 non-hemolytic isolates from competition assays at day 5

controlledIPD-YESFeb 2026View details →

Adjunctive Fosfomycin for Treatment of Staphylococcus Aureus Bacteraemia

ClinicalTrials.gov study NCT06695832. IPD Sharing: YES. Countries: 3. Publications: 21.

closedIPD-NOFeb 2026View details →

Persistent Methicillin Resistant Staphylococcus Aureus Eradication Protocol (PMEP)

ClinicalTrials.gov study NCT01594827. IPD Sharing: NO. Countries: 1. Publications: 4.

Methicillin-resistant Staphylococcus Aureus (MRSA) Skin and Soft Tissue Infection (SSTI) Prevention in Military Trainees

ClinicalTrials.gov study NCT01105767. IPD Sharing: Not stated. Countries: 1. Publications: 8.

Comparison of Telavancin and Vancomycin for Hospital-acquired Pneumonia Due to Methicillin-resistant Staphylococcus Aureus

ClinicalTrials.gov study NCT00124020. IPD Sharing: Not stated. Countries: 1. Publications: 3.

The Impact of Treating Staphylococcus Aureus Infection and Colonization on the Clinical Severity of Atopic Dermatitis

ClinicalTrials.gov study NCT00179959. IPD Sharing: Not stated. Countries: 1. Publications: 1.

Cluster Randomized Trial of Hospitals to Assess Impact of Targeted Versus Universal Strategies to Reduce Methicillin-resistant Staphylococcus Aureus (MRSA) in Intensive Care Units (ICUs)

ClinicalTrials.gov study NCT00980980. IPD Sharing: Not stated. Countries: 1. Publications: 3.

Skin Structure Infections With Suspected or Proven Methicillin-Resistant Staphylococcus Aureus (MRSA)

ClinicalTrials.gov study NCT00087490. IPD Sharing: Not stated. Countries: 16. Publications: 2.

Prospective Study of Methicillin-Resistant Staphylococcus Aureus (MRSA) Among HIV-Infected Persons

ClinicalTrials.gov study NCT00631566. IPD Sharing: Not stated. Countries: 1. Publications: 4.

Comparison of Telavancin and Vancomycin for Complicated Skin and Skin Structure Infections With a Focus on Methicillin-resistant Staphylococcus Aureus

ClinicalTrials.gov study NCT00107978. IPD Sharing: Not stated. Countries: 1. Publications: 3.