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FIGURE 9 in A new species of Temnocephala (Platyhelminthes) with an unusual pharynx, including an amendment of the diagnosis of the genus

FIGURE 9. Hyperparasites. (a) Plerocercoid larva, showing four suckers. Scale bar: 50 µm. (b) Nematode (left) and plerocercoid larva (right). Scale bar: 100 µm.

FIGURE 7 in A new species of Temnocephala (Platyhelminthes) with an unusual pharynx, including an amendment of the diagnosis of the genus

FIGURE 7. Penial stylet of of Temnocephala gargantua sp. nov. (a) Whole stylet. Scale bar: 50 µm. (b) Introvert. Scale bar: 15 µm.

FIGURE 6 in A new species of Temnocephala (Platyhelminthes) with an unusual pharynx, including an amendment of the diagnosis of the genus

FIGURE 6. Reproductive complex of Temnocephala gargantua sp. nov. (a) Reconstruction of the whole complex from a dissection. Scale bar: 100 µm. (b) Reconstruction of the female reproductive complex based on serial sagittal sections. Scale bar: 100 µm. (c) Detail of the sphincter in the female reproductive complex. The asterisk marks the thinner side of the sphincter. Scale bar: 30 µm. Abbreviations: at, atrium; cv, contractile vesicle; go, gonopore; ov, ovary; ps, penial stylet; rv, resorbiens vesicle; sp, sphincter; sr, seminal receptacle; sv, seminal vesicle; vd, vitelline duct; vg, vagina.

FIGURE 5 in A new species of Temnocephala (Platyhelminthes) with an unusual pharynx, including an amendment of the diagnosis of the genus

FIGURE 5. Histological details of the pharynx and oesophagus of Temnocephala gargantua sp. nov. (a) Anterior sphincter. Scale bar: 50 µm. (b) Posterior sphincter. Scale bar: 50 µm. (c) Inner circular musculature or the pharynx. Scale bar: 50 µm. (d) Cell with clear cytoplasm among radial muscle fibers. Scale bar: 20 µm. (e) Cell with dark cytoplasm among radial muscle fibers. Scale bar: 20 µm. (f) Circular muscle fibers surrounding the oesophagus. Scale bar: 30 µm. Abbreviations: cm, circular musle fibers; ep, epidermis; in, intestine; oe, oesophagus.

FIGURE 4 in A new species of Temnocephala (Platyhelminthes) with an unusual pharynx, including an amendment of the diagnosis of the genus

FIGURE 4. Sagittal section of the pharynx of Temnocephala gargantua sp. nov. Scale bar: 100 µm. Abbreviations: as, anterior sphincter; cm, circular musculature; ep, epidermis; lm, longitudinal musculature; mo, mouth; ps, posterior sphincter.

FIGURE 3 in A new species of Temnocephala (Platyhelminthes) with an unusual pharynx, including an amendment of the diagnosis of the genus

FIGURE 3. Sagittal section of Temnocephala gargantua sp. nov. Scale bar: 300 µm. Abbreviations: at, atrium; gl, glands surrounding the distal portion of the female reproductive system; go, gonopore; in, intestine; mo, mouth; ov, ovary; ph, pharynx; rv, resorbiens vesicle; sp, sphincter; su, sucker; tn, tentacle; vi, vitellaria.

FIGURE 2 in A new species of Temnocephala (Platyhelminthes) with an unusual pharynx, including an amendment of the diagnosis of the genus

FIGURE 2. Excretory syncytia of Temnocephala gargantua sp. nov. Abbreviations: ep, excretory pore; es, excretory syncytium.

FIGURE 1 in A new species of Temnocephala (Platyhelminthes) with an unusual pharynx, including an amendment of the diagnosis of the genus

FIGURE 1. Temnocephala gargantua sp. nov. in ventral view. (a) Holotype, showing Stratiodrilus' hard parts in the intestine. Scale bar: 400 µm. (b) Diagram of the general organization of the body. Scale bar: 500 µm. Abbreviations: ev, excretory vesicle; hg, Haswell's glands; ph, pharynx; sg, sucker glands; su, sucker; te, testes; tg, tentacular glands; vi, vitellaria.

FIGURE 6 in New species of Temnocephala (Platyhelminthes, Temnocephalidae) ectosymbiont on decapod crustaceans Aegla jarai (Aeglidae) from Brazil and Samastacus spinifrons (Parastacidae) from Chile

FIGURE 6. Photomicrographs of cirri of Temnocephala catarinensis sp. nov. from Samastacus spinifrons, seen with Nomarski's DIC microscopy. A. Entire cirrus (lateral view), showing the evident curvature. B. Entire cirrus (dorsal or ventral view), showing that the curvature is less evident when the cirrus is in this position and the proximal limit of the introvert (head arrow). Scale bars: 25 μm. C–H. Introvert seen in different focusing planes, showing the proximal limit of the introvert (arrow), scale bars: 10 μm.

FIGURE 3 in New species of Temnocephala (Platyhelminthes, Temnocephalidae) ectosymbiont on decapod crustaceans Aegla jarai (Aeglidae) from Brazil and Samastacus spinifrons (Parastacidae) from Chile

FIGURE 3. Line drawings of holotype of Temnocephala catarinensis sp. nov. from Aegla jarai. A. Adult specimen, ventral view, scale bar: 250 μm. B. Cirrus showing the proximal limit of the introvert (arrow), scale bar: 20μm. C. Partial line drawings of the male reproductive system. D. Partial line drawings of the female reproductive system. Scale bars: 50 μm. (adhesive disc (ad), anterior testes (at), anterior portion of the vaginal sphincter (avs), cirrus (c), deferent vessels (dd), excretory vesicle (ev), genital atrium (ga), genital pore (gp), Haswell glands (hg), intestine (i), mouth (m), ovary (ov), paranephrocytes (pa), prostatic bulb (pb), prostatic cells (pc), pharynx (ph), prostatic secretions (ps), posterior testis (pt), posterior portion of the vaginal sphincter (pvs), seminal vesicle (sv), tentacles (t), vagina (va), vitelline duct (vd), vitelline glands (vg), vesicula intermedia (vi), vesicula resorbens (vr)).

FIGURE 1. Aegla jarai A in New species of Temnocephala (Platyhelminthes, Temnocephalidae) ectosymbiont on decapod crustaceans Aegla jarai (Aeglidae) from Brazil and Samastacus spinifrons (Parastacidae) from Chile

FIGURE 1. Aegla jarai A. Merus part of the cheliped showing unhatched (black head arrow) and hatched eggs (white head arrows) of Temnocephala catarinensis sp. nov. Scale bar: 1 mm. B. Posterior portion of the left side of the body showing posterior limit of the rhabditogenic glands (black head arrows) and the posterior limit of the disc glands (white head arrows) of Temnocephala catarinensis sp. nov., Scale bar: 250 μm. C. Ventral view of adult specimens of Temnocephala catarinensis sp. nov. from Aegla jarai. Scale bar: 250 μm.

FIGURE 2 in New species of Temnocephala (Platyhelminthes, Temnocephalidae) ectosymbiont on decapod crustaceans Aegla jarai (Aeglidae) from Brazil and Samastacus spinifrons (Parastacidae) from Chile

FIGURE 2. Dorsal view of Temnocephala catarinensis sp. nov. observed with SEM, showing details of the epidermal 'excretory' syncytial plates (EPs). A. Specimen from Aegla jarai showing right EP and nephridiopore (n). B. Specimen from Samastacus spinifrons showing EPs and nephridiopores (n). C. Detail of the right EP from Samastacus spinifrons showing its limits and nephridiopore (n). Scale bars: 100 μm.

FIGURE 4 in New species of Temnocephala (Platyhelminthes, Temnocephalidae) ectosymbiont on decapod crustaceans Aegla jarai (Aeglidae) from Brazil and Samastacus spinifrons (Parastacidae) from Chile

FIGURE 4. Partial reproductive system of an adult specimen of Temnocephala catarinensis sp. nov. from Aegla jarai. A. Partial female and male reproductive system B. Partial female reproductive system C. Partial male reproductive system. Scale bars: 50 μm. (anterior portion of the vaginal sphincter (avs), cirrus (c), genital atrium (ga), genital pore (gp), ovary (ov), prostatic bulb (pb), posterior portion of the vaginal sphincter (pvs), seminal vesicle (sv), vagina (va), vesicula intermedia (vi), vesicula resorbens (vr)).

Figure 4 from: Lenis C, Ruiz F, Muskus C, Marcilla A, Vélez I (2020) A new flatworm species of Temnocephala (Rhabdocoela, Temnocephalidae) ectosymbiont on the freshwater crab Valdivia serrata (Decapoda, Trichodactylidae) from Amazonas, Colombia. ZooKeys 918: 1-14. https://doi.org/10.3897/zookeys.918.38201

Figure 4 Details of the reproductive system of Temnocephala ivandarioi sp. nov. A, B partial female reproductive system, showing: anterior portion of the distal vaginal sphincter (advs), anterior portion of the proximal vaginal sphincter (apvs), distal vaginal sphincter (dvs), genital atrium (ga), posterior portion of the distal vaginal sphincter (pdvs), posterior portion of the proximal vaginal sphincter (ppvs), proximal vaginal sphincter (pvs), ovary (ov), vagina (va), and vesicula resorbens (vr) C, D partial male reproductive system, showing: cirrus (c), prostatic bulb (pb), seminal vesicle duct (svd), seminal vesicle (sv), and vasa deferentia (vd) E, F cirrus introvert observed in different focusing planes, view of the circle of sclerites (sp) in the distal portion of the introvert and the smooth portion (smp) in the proximal limit of the introvert (pli). Scale bars: 50 μm (A–D); 20 μm (E, F).

Figure 3 from: Lenis C, Ruiz F, Muskus C, Marcilla A, Vélez I (2020) A new flatworm species of Temnocephala (Rhabdocoela, Temnocephalidae) ectosymbiont on the freshwater crab Valdivia serrata (Decapoda, Trichodactylidae) from Amazonas, Colombia. ZooKeys 918: 1-14. https://doi.org/10.3897/zookeys.918.38201

Figure 3 Temnocephala ivandarioi sp. nov. details of epidermal excretory syncytial plates (DLSPs) and eggs A antero-lateral area observed with SEM showing leftmost tentacle and left DLSP, arrow showing contour and position of excretory pores (n) B line drawing of entire specimen showing the DLSPsC egg observed with SEM showing the filament (fi), peduncle (pe), and plane of fracture of the operculum (pf) D line drawing of a whole egg showing the oblique fracture plane to the longitudinal axis of the egg E live adult specimen showing red eyespot pigment F unhatched egg showing the filament (fi) G, H live eggs deposited on branchial chambers of V. serrata. Scale bars: 100 μm (A–F); 1mm (G, H).

Figure 6 from: Lenis C, Ruiz F, Muskus C, Marcilla A, Vélez I (2020) A new flatworm species of Temnocephala (Rhabdocoela, Temnocephalidae) ectosymbiont on the freshwater crab Valdivia serrata (Decapoda, Trichodactylidae) from Amazonas, Colombia. ZooKeys 918: 1-14. https://doi.org/10.3897/zookeys.918.38201

Figure 6 Diagrams of the cirrus of the species of Temnocephala associated with trichodactylid crabs. Terminology based on the cirrus structure (see comparative notes and Fig. 5). ATemnocephala ivandarioi sp. nov. (present study) BTemnocephala lanei Pereira & Cuocolo, 1941 CTemnocephala longivaginata Seixas, Amato & Amato, 2011 DTemnocephala lutzi Monticelli, 1913 (Amato et al. 2005) ETemnocephala microdactyla Monticelli, 1903 FTemnocephala pignalberiae Dioni, 1967 (Amato et al. 2010) GTemnocephala santafesina Dioni, 1967 HTemnocephala trapeziformis Amato, Amato & Seixas, 2006 ITemnocephala travassosfilhoi Pereira & Cuocolo, 1941. Key: Shaft [shape: CO–cone; FU–funnel]; [curvature: CU–curved up; ST–straight; CD–curved down]. Introvert [shape: CY–cylindrical; CO–cone; SC–scoop]; [armed with: UN–unarmed; SP–spines; SL–sclerites; RI–ridges; ND–not described]; [angle: NO–not oblique; VO–very oblique]; [curvature: NC–not curved; FC–forward curved; BC–backward curved].

Figure 5 from: Lenis C, Ruiz F, Muskus C, Marcilla A, Vélez I (2020) A new flatworm species of Temnocephala (Rhabdocoela, Temnocephalidae) ectosymbiont on the freshwater crab Valdivia serrata (Decapoda, Trichodactylidae) from Amazonas, Colombia. ZooKeys 918: 1-14. https://doi.org/10.3897/zookeys.918.38201

Figure 5 Diagrams showing the terminology used to describe the cirrus of the species of Temnocephala (terminology updated from Sewell et al. (2007), Seixas et al. (2011), Garcés et al. (2013), and Ponce de León et al. (2015); diagrams modified from Sewell et al. 2007: 205, fig. 2).

Figure 1 from: Lenis C, Ruiz F, Muskus C, Marcilla A, Vélez I (2020) A new flatworm species of Temnocephala (Rhabdocoela, Temnocephalidae) ectosymbiont on the freshwater crab Valdivia serrata (Decapoda, Trichodactylidae) from Amazonas, Colombia. ZooKeys 918: 1-14. https://doi.org/10.3897/zookeys.918.38201

Figure 1 Temnocephala ivandarioi sp. nov. and Valdivia serrataA paratype of Temnocephala ivandarioi sp. nov. showing an egg, ventral view B adult paratype stained in Meyer´s paracarmine C holotype stained in Borax carmine D male specimen of V. serrataE abdomen F gonopods, lateral view. Scale bars: 200 µm (A–C); 10 mm (D–F).

Figure 2 from: Lenis C, Ruiz F, Muskus C, Marcilla A, Vélez I (2020) A new flatworm species of Temnocephala (Rhabdocoela, Temnocephalidae) ectosymbiont on the freshwater crab Valdivia serrata (Decapoda, Trichodactylidae) from Amazonas, Colombia. ZooKeys 918: 1-14. https://doi.org/10.3897/zookeys.918.38201

Figure 2 Temnocephala ivandarioi sp. nov. A adult specimen diagram, showing adhesive disk (ad), anterior testes (at), cyanophilous glands (cg), disk glands (dg), excretory vesicle (ev), Haswell´s glands (hg), intestinal sac (i), mouth (m), pharynx (ph), posterior testes (pt), rhabditogenic glands (rg), tentacles (t), and vitellarium (v) B reproductive system, showing female reproductive complex: anterior portion of the distal vaginal sphincter (advs), anterior portion of the proximal vaginal sphincter (apvs), genital atrium (ga), genital pore (gp), posterior portion of the distal vaginal sphincter (pdvs), posterior portion of the proximal vaginal sphincter (ppvs), ovary (ov), vagina (va), and resorbens vesicle (vr); and male reproductive organs: cirrus (c), prostatic bulb (pb), prostatic vesicle (pv), seminal vesicle (sv), and vasa deferentia (vd) C line drawing of cirrus in different focusing planes, showing the sclerites portion of the introvert (sp), and proximal limit of the introvert (arrows). Scale bars: 200 µm (A); 100 µm (B); 20 μm (C).

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FIGURE 5 in New species of Temnocephala (Platyhelminthes, Temnocephalidae) ectosymbiont on decapod crustaceans Aegla jarai (Aeglidae) from Brazil and Samastacus spinifrons (Parastacidae) from Chile

FIGURE 5. Photomicrographs of cirri of Temnocephala catarinensis sp. nov. from Aegla jarai, seen with Nomarski's DIC microscopy. A. Entire cirrus (lateral view), showing the evident curvature, scale bar: 25 μm. B. Entire cirrus (dorsal or ventral view), showing that the curvature is less evident when the cirrus is in this position, the proximal limit of the introvert (arrow) and the shaft rim (head arrow), typical of adult worms, scale bar: 20μm. C–K. Introvert seen in different focusing planes, showing the proximal limit of the introvert (arrow), scale bars: 10 μm.