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29 results for “amphibian larvae”
Physiology and acclimation potential are tuned with phenology in larvae of a prolonged breeder amphibian
Due to the speed of climate changes, rapid buffering mechanisms such as phenotypic plasticity – which may depend on breeding phenology – could be key to avoid extinction. The links between phenology and plasticity, however, remain understudied. Here we explored the matching between phenology and the thermal sensitivity of standard (SMR) and routine metabolic rates (RMR), metabolic scope (i.e. the difference between RMR and SMR), survival and growth-development trajectories in larvae of a prolonged breeder amphibian (Alytes almogavarii) acclimated to 10 and 20ºC, belonging to three cohorts: autumn pre-overwintering, autumn overwintering and spring tadpoles. At 20ºC, survival of autumn pre-overwintering larvae was lower than for the rest. Although all cohorts showed acclimation potential, patterns for SMR and RMR differed, leading to differences in metabolic scope. Regardless of temperature, overwintering tadpoles arrested growth and development, while pre-overwintering and spring tadpoles showed higher growth and development at 20ºC. At 10ºC pre-overwintering tadpoles allocated more energy to development compared to spring tadpoles to advance development before winter. Overall, we demonstrate that the effects of temperature depend on phenology, consistent with future, expected thermal regimes. This suggests that extreme events can yield different vulnerability to climate change within populations (e.g., associated to discrete within-year cohorts), and not only between species or populations.
Data from: Skin swabbing of amphibian larvae yields sufficient DNA for efficient sequencing and reliable microsatellite genotyping
Skin swabbing, a minimally invasive DNA sampling method recently developed on adult amphibians, was tested on larvae of fire salamanders (Salamandra salamandra). The quality and quantity of the sampled DNA was evaluated by (i) measuring DNA concentration in DNA extracts, (ii) sequencing part of the mtDNA cytochrome b gene (692 bp) and (iii) genotyping eight polymorphic nuclear microsatellite loci. The multiple-tubes approach was used for calculating allelic dropout (ADO) and false allele (FA) rates to evaluate the reliability of the genotypes. DNA extracts from tissue samples of road-killed individuals were included in the study as positive controls. Our results showed that skin swabs of fire salamander larvae can provide DNA in sufficient quantity and quality, as sequencing was successful and no allelic dropouts or false alleles were detected. This method, tested for the first time on amphibian larvae, has proven to be an efficient and reliable alternative to the controversial tail fin clipping procedure.
Data from: The response of amphibian larvae to environmental change is both consistent and variable
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Do epigenetic changes drive corticosterone responses to alarm cues in larvae of an invasive amphibian?
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Data from: Where have all the tadpoles gone? Individual genetic tracking of amphibian larvae until adulthood.
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Data from: Skin swabbing of amphibian larvae yields sufficient DNA for efficient sequencing and reliable microsatellite genotyping
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Data from: Interacting effects of predation risk and resource level on escape speed of amphibian larvae along a latitudinal gradient
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Physiology and acclimation potential are tuned with phenology in larvae of a prolonged breeder amphibian
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Data from: Body size, swimming speed, or thermal sensitivity? Predator-imposed selection on amphibian larvae
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.