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525 results for “amplification”
Fig.1 in Optimisation Of Dna Extraction And Rapd-Pcr Amplification For Population Genetic Analysis Of Daphnia Cucullata Sars, 1862 (Crustacea: Cladocera)
Fig.1. RAPD fingerprints results from different samples of Daphnia cucullata with primers OPA-03 and OPA-05 (M- marker, 1-16 runners- different samples of Daphnia cucullata; 17- control) using RAPD-PCR 10 × Taq buffer with KCl.
Data and the simulation script for "Through-Bond and Through-Space Radiofrequency Amplification by Stimulated Emission of Radiation"
<p>The dataset includes NMR spectra and the MatLab script for the simulations of RASER triggered via Nuclear Overhauser Effect for the "Through-Bond and Through-Space Radiofrequency Amplification by Stimulated Emission of Radiation" paper (under revision at the moment of the dataset publication).</p>
Text-fig. 4. Electrophoresis after amplification: Electrophoretical analysis of mitochondrial DNA. mtDNA sequences were amplified by primers F15.412 and R16.169 (450 bp), R16.269 (550 bp), R16.519 (800 bp). Lane 1 are primers F15.412 + R16.169, lane 2 primers F15.412 + R16.269, lane 3 primers F15.412 + R16.519, NC – negative control – water, L – 100 bp DNA ladder (band size from 100 bp to 1500 bp). in Genetic Analysis Of Possibly The Oldest Greyhound Remains Within The Territory Of The Czech Republic As Proof Of A Local Elite Presence At Chotěbuz-Podobora Hillfort In The 8 -9 Century Ad
Text-fig. 4. Electrophoresis after amplification: Electrophoretical analysis of mitochondrial DNA. mtDNA sequences were amplified by primers F15.412 and R16.169 (450 bp), R16.269 (550 bp), R16.519 (800 bp). Lane 1 are primers F15.412 + R16.169, lane 2 primers F15.412 + R16.269, lane 3 primers F15.412 + R16.519, NC – negative control – water, L – 100 bp DNA ladder (band size from 100 bp to 1500 bp).
Data for "Bulk and fracture process zone contribution to the rate-dependent adhesion amplification in viscoelastic broad-band materials"
<p>This dataset contains all the data generated for the publication</p> <p>[1] A. Maghami, Q. Wang, M. Tricarico et al., Bulk and fracture process zone contribution to the rate-dependent adhesion amplification in viscoelastic broad-band materials. Journal of the Mechanics and Physics of Solids (2024), doi:<br>https://doi.org/10.1016/j.jmps.2024.105844.</p> <p><br>The provided data are those which appear in the figures of Ref. [1]. Data are stored using informative named structures in a ".mat" file. The data are easily accessible through the Commercial Software MATLAB (© 1994-2023 The MathWorks, Inc.) or by using the free software GNU Octave. An exemplary code for loading and plotting the data contained in "data_Sphere_Viscoelastic_BroadBand.mat" is the following:</p> <p>%%%%%%%%%%%%%</p> <div> <div> <div> <div>clc</div> <div>clearvars</div> <div> </div> <div>close all</div> <div> </div> <div> </div> <div>% Create a new figure</div> <div>load('data_Sphere_Viscoelastic_BroadBand.mat');</div> <div> </div> <div> </div> <div>figure</div> <div> </div> <div>% Choose one of the following names:</div> <div>% Fig_3_a, Fig_3_b, Fig_3_c,</div> <div>% Fig_4, Fig_5_a, Fig_5_b, Fig_5_b_inset,</div> <div>% Fig_6, Fig_6_inset, Fig_7, Fig_7_inset,</div> <div>% Fig_8_a, Fig_8_b, Fig_10_a, Fig_10_b</div> <div> </div> <div>name=Fig_3_a;</div> <div>% Note: some plots in the manuscript are in log space.</div> <div> </div> <div>% Plot the data from the structure</div> <div> </div> <div> </div> <div>for i = 1:numel(name.x) hold on</div> <div> </div> <div>x_cell = name.x(i);</div> <div>y_cell = name.y(i);</div> <div>x = cell2mat(x_cell);</div> <div>y = cell2mat(y_cell);</div> <div> </div> <div>plot(x, y);</div> <div>end</div> <div> </div> <div> </div> <div> </div> </div> %%%%%%%%%%%%%</div> </div> <p>The code can be easily adapted for plotting the curves of all the figures contained in Ref. [1]. </p>
Developer-Centric Test Amplification: The Interplay Between Automatic Generation and Human Exploration --- Appendix
<p>This online appendix contains the accumulated code occurences during the interviews performed to evaluate our developer-centric test amplification approach and the TestCube plugin. In addition, it documents the inter-rater-reliability analysis we performed.</p>
Data from: Refining the evolutionary time machine: an assessment of whole genome amplification using single historical Daphnia eggs
<p>This dataset contains the original raw sequence files used in the associated publication "Refining the evolutionary time machine: an assessment of whole genome amplification using single historical <em>Daphnia</em> eggs" by O'Grady, Dhandapani, Colbourne & Frisch in Molecular Ecology Resources. DOI:10.1111/1755-0998.13524</p> <p> </p> <p>(filename > name used in associated publication)</p> <p>DF1_ATTACTC-GGCTCTG_L008_R1_001.fastq.gz -> DM1<br> DF2_TCCGGAG-GGCTCTG_L008_R1_001.fastq.gz -> DM2<br> DF3_CGCTCAT-GGCTCTG_L008_R1_001.fastq.gz -> DM3<br> DF4_GAGATTC-GGCTCTG_L008_R1_001.fastq.gz -> DM4<br> DF5_CTGAAGC-GGCTCTG_L008_R1_001.fastq.gz -> DM5<br> DF11_CTGAAGC-AGGCGAA_L008_R1_001.fastq.gz -> DM6<br> DF12_TAATGCG-AGGCGAA_L008_R1_001.fastq.gz -> DM7</p> <p>DF6_TAATGCG-GGCTCTG_L008_R1_001.fastq.gz -> DP1<br> DF7_ATTACTC-AGGCGAA_L008_R1_001.fastq.gz -> DP4<br> DF8_TCCGGAG-AGGCGAA_L008_R1_001.fastq.gz -> DP5<br> DF9_CGCTCAT-AGGCGAA_L008_R1_001.fastq.gz -> DP2<br> DF10_GAGATTC-AGGCGAA_L008_R1_001.fastq.gz -> DP3</p> <p>170426_E00397_0064_BHJ2JWALXX_2_TP-D7-004_1.fastq.gz -> DP6 (fw)<br> 170426_E00397_0064_BHJ2JWALXX_2_TP-D7-004_2.fastq.gz -> DP6 (rv)<br> 170426_E00397_0064_BHJ2JWALXX_2_TP-D7-010_1.fastq.gz -> DP7 (fw)<br> 170426_E00397_0064_BHJ2JWALXX_2_TP-D7-010_2.fastq.gz -> DP7 (rv)</p> <p>170426_E00397_0064_BHJ2JWALXX_2_TP-D7-005_1.fastq.gz -> DP8 (fw)<br> 170426_E00397_0064_BHJ2JWALXX_2_TP-D7-005_2.fastq.gz -> DP8 (rv)<br> 170426_E00397_0064_BHJ2JWALXX_2_TP-D7-006_1.fastq.gz -> DP9 (fw)<br> 170426_E00397_0064_BHJ2JWALXX_2_TP-D7-006_2.fastq.gz -> DP9 (rv)</p>
Model data used for the paper: "Heat extremes driven by amplification of phase-locked circumglobal waves forced by topography in an idealized atmospheric model"
<p>Atmospheric model output to reproduce the results of the study: "<strong>Heat Extremes Driven by Amplification of Phase-Locked Circumglobal Waves Forced by Topography in an Idealized Atmospheric Model" </strong>published in Geophysical Research letters.<br> <br> Authors: B. Jiménez-Esteve. K. Kornhuber and D. I.V. Domeisen <br> <br> DOI: <a href="https://doi.org/10.1029/2021GL096337">https://doi.org/10.1029/2021GL096337</a><br> <br> For more information about the model setup and the design of the experiments please refer to the above publication.</p>
Data from: Cost-saving population genomic investigation of Daphnia longispina complex resting eggs using whole genome amplification and pre-sequencing screening
<p>This dataset contains all paired MiSeq sequences that were generated for the study "Cost-saving population genomic investigation of<em> Daphnia longispina</em> complex resting eggs using whole genome amplification and pre-sequencing screening" by Nickel and Cordellier.</p> <p>The sample names used in the study and the associated file names are explained in the table<strong> </strong>"Study_sample_names.xlsx"</p> <p> </p>
Growth form and leaf habit drive contrasting effects of Arctic amplification in long-lived woody species
<p><strong>Raw_and_STD_Chronologies.txt</strong> contains the raw (suffix "_raw") and indexed (suffix "_std") ring-width chronology derived from seven species (<em>Juniperus communis</em> L., <em>Betula nana</em> L., <em>Salix lanata</em> L., <em>Picea abies</em> L., <em>Pinus contorta</em> Douglas, <em>Betula pubescens</em> Ehrh., and <em>Sorbus aucuparia</em> L.) collected from ten sites located across Iceland. In the file, each column addresses the chronology belonging to a precise site and species, coded following the one used in the International Tree-Ring Data Bank (ITRDB) as listed in Grissino_Mayer (1993). This data was used to model growth trends and to perform climate-growth associations over the 1967-2018 period.</p>
eDNAssay: a machine learning tool that accurately predicts qPCR cross-amplification
Open the record for dataset details and reuse information.
Dataset for: Ultrarapid detection of SARS-CoV-2 RNA using a reverse transcription-free exponential amplification reaction, RTF-EXPAR
Open the record for dataset details and reuse information.
Optomechanical microwave amplification without mechanical amplification
<p>High-gain and low-noise signal amplication is a valuable tool in various cryogenic microwave experiments. A microwave optomechanical device, in which a vibrating capacitor modulates the frequency of a microwave cavity, is one technique that is able to amplify microwave signals with high gain and large dynamical range. Such optomechanical ampliers typically rely on strong backaction of microwave photons on the mechanical mode achieved in the sideband-resolved limit of optomechanics. Here, we observe microwave amplication in an optomechanical cavity in the extremely unresolved sideband limit. A large gain is observed for any detuning of the single pump tone within the cavity linewidth, a clear indication that the amplication is not induced by dynamical backaction. By being able to amplify for any detuning of the pump signal, the amplication center frequency can be tuned over the entire range of the broad cavity linewidth. Additionally, by providing microwave amplication without mechanical amplication, we predict that using this scheme it is possible to achieve near-quantum-limited microwave amplication despite a large thermal occupation of the mechanical mode.</p>
Gene family amplification facilitates adaptation in freshwater Unionid bivalve Megalonaias nervosa
<p>As organisms are faced with intense rapidly changing selective pressures, new genetic material is required to facilitate adaptation. Among sources of genetic novelty, gene duplications and transposable elements (TEs) offer new genes or new regulatory patterns that can facilitate evolutionary change. With advances in genome sequencing it is possible to gain a broader view of how gene family proliferation and TE content evolve in non-model species when populations become threatened. Freshwater bivalves (Unionidae) currently face severe anthropogenic challenges. Over 70% of species in the United States are threatened, endangered or extinct due to pollution, damming of waterways, and overfishing. We have created a reference genome for M. nervosa to determine how genome content has evolved in the face of these widespread environmental challenges. We observe a burst of recent transposable element proliferation causing a 382 Mb expansion in genome content. Gene family expansion is common, with a duplication rate of 1.16 x 10^-8 per gene per generation. Cytochrome P450, ABC transporters, Hsp70 genes, von Willebrand proteins, chitin metabolism genes, mitochondria eating proteins, and opsin gene families have experienced significantly greater amplification and show signatures of selection. We use evolutionary theory to assess the relative contribution of SNPs and duplications in evolutionary change. Estimates suggest that gene family evolution may offer an exceptional substrate of genetic variation in M. nervosa, with Psgv=0.185 compared with Psgv=0.067 for single nucleotide changes. Hence, we suggest that gene family evolution is a source of "hopeful monsters" within the genome that facilitate adaptation.</p>
DATA - Effects of Hearing Aid Amplification on Robust Neural Coding of Speech
<p>This data is presented in the following dissertation:<br /> Effects of Hearing Aid Amplification on Robust Neural Coding of Speech<br /> http://docs.lib.purdue.edu/open_access_dissertations/190/</p> <p>The code for analyzing this data is here:<br /> http://dx.doi.org/10.5281/zenodo.49296</p> <p>The data is organized as follows:</p> <ul> <li>The main file is Research.zip. This contains the directory structure within the "Research" folder.</li> <li>The contents of PhaseModulation.zip should be moved to "Research\Matlab"</li> <li>Everything else should be moved to "Research\Matlab\Vowel_STMP\ExpData"</li> </ul> <p> </p>
Data and code for the paper titled "Changing Northern Hemisphere Weather Linked to Warming Amplification in High Mountain Asia"
<p>This is the data and code for the paper titled "Changing Northern Hemisphere Weather Linked to Warming Amplification in High Mountain Asia", which has published in</p> <div> <div><em>Communications Earth & Environment, </em>DOI : 10.1038/s43247-025-02883-0.</div> </div>
Dataset: Survey on the actual situation of antibiotic resistant bacteria detection by nucleic acid amplification test in clinical microbiology laboratories at hospitals in Japan: Online survey of participants in workshops organized by the Nara Association of Medical Technologists
<p>The coronavirus disease 2019 pandemic has led to the widespread use of the nucleic acid amplification test (NAAT), along with an increase in demand for SARS-CoV-2 tests. NAAT has been used to detect antimicrobial resistance (AMR) genes since before the pandemic, but the test has been performed in a limited number of facilities. We investigated the current status and background of Japanese clinical laboratories by surveying the implementation of genotypic AST in NAAT, which has become widespread owing to the pandemic. This means that 59% of the respondents possessed NAAT and were using it for genotypic AST. GeneXpert and FilmArray were introduced in the majority of cases (62.5% and 82.6%, respectively), with the pandemic as the trigger. More than half of the respondents cited “rapid detection” (56.0%) and “ICT requests” (52.4%) as the reasons for introducing the system. Regarding usefulness, “contribution to infectious disease treatment” (74.1%) showed the highest percentage. Among the respondents who cited “not implemented”, the most frequent responses were “I have no plans, but I want to do it.” (38.1%) and “would do so if requested by a physician” (33.3%). The most common reason for not implementing the system was concern about increased workload (52.9%). We believe that this is due to changes in the working environment caused by the pandemic and the characteristics of Japanese society. In the future, to promote the adoption of genotypic AST, it will be necessary to approach it through reports on its usefulness from domestic facilities, and simultaneously, improving and enhancing efficiency in work processes will also be essential.</p>
Dissecting muscle power output: Evidence of multi-scale power amplification in skeletal muscle
<p class="MsoNormal">Many animals use a combination of skeletal muscle and elastic structures to amplify power output for fast motions. Among vertebrates, tendons in series with skeletal muscle are often implicated as the primary power-amplifying spring, but muscles contain elastic structures at all levels of organization, from the muscle tendon to the extracellular matrix to elastic proteins within sarcomeres. The present study used <em>ex vivo</em> muscle preparations in combination with high-speed video to quantify power output, as the product of force and velocity, at several levels of muscle organization to determine where power amplification occurs. Dynamic ramp shortening contractions in isolated frog flexor digitorum superficialis brevis were compared with isotonic power output to identify power amplification within muscle fibers, the muscle belly, free tendon and elements external to the muscle tendon. Energy accounting revealed that artifacts from compliant structures outside of the muscle–tendon unit contributed significant peak instantaneous power. This compliance included deflection of clamped bone that stored and released energy contributing 195.22±33.19 W kg<sup>−1</sup> (mean±s.e.m.) to the peak power output. In addition, we found that power detected from within the muscle fascicles for dynamic shortening ramps was 338.78 ±16.03 W kg<sup>−1</sup>, or nearly twice the maximum isotonic power output of 195.23±8.82 W kg<sup>−1</sup>. Measurements of muscle belly and muscle–tendon unit also demonstrated significant power amplification. These data suggest that intramuscular tissues, as well as bone, have the capacity to store and release energy to amplify whole-muscle power output.</p>
Ground motion amplification of the Santiago basin
<div> <div>Code that generates an approximate distribution of strong ground motion amplification in the Santiago Basin. A combination of two-dimensional Gaussians is used to resemble the distribution found by Pilz et al. (2011) [https://doi.org/10.1111/j.1365-246X.2011.05183.x].</div> <div>The parameters used were selected through trial and error until a distribution was obtained that roughly resembled those found by Pilz. This code requires the data available at https://doi.org/10.5281/zenodo.10050539.</div> </div>
[Dataset] Polar and Topographic Amplifications of Inter-model Spread of Surface Temperature in Climate Models
<p>All the raw CMIP5 and CMIP6 model data used in this work are available at <a href="https://data.ceda.ac.uk/badc/cmip5/data/cmip5/output1">https://data.ceda.ac.uk/badc/cmip5/data/cmip5/output1</a> and <a href="https://esgf-node.llnl.gov/search/cmip6/">https://esgf-node.llnl.gov/search/cmip6/</a> respectively.</p> <p>The data uploaded here is the processed data that support and lead to the described results in the manuscript entitled "Polar and Topographic Amplifications of Inter-model Spread of Surface Temperature in Climate Models"</p> <p>Figure numbers shown in brackets are associated figures in the manuscript.</p> <p><br> cmip5_sdev.nc (the first row of figure 1 )<br> cmip6_sdev.nc (the second row figure 1 )</p> <p>cmip6_np_decompose.nc (figure 2 and figure 6)<br> cmip6_sp_decompose.nc (figure 3 and figure 7)<br> cmip6_tp_decompose.nc (figure 4 and figure 8)<br> cmip6_clt.nc (figure 5)<br> cmip6_np_energy_transport.nc (the first column of figure 9)<br> cmip6_sp_energy_transport.nc (the second column of figure 9)<br> cmip6_tp_energy_transport.nc (the third column of figure 9)</p> <p><br> cmip5_np_decompose.nc (figure 10 and figure 14 in appendix)<br> cmip5_sp_decompose.nc (figure 11 and figure 15 in appendix)<br> cmip5_tp_decompose.nc (figure 12 and figure 16 in appendix)<br> cmip5_clt.nc (figure 13 in appendix)<br> cmip5_np_energy_transport.nc (the first column of figure 17 in appendix)<br> cmip5_sp_energy_transport.nc (the second column of figure 17 in appendix)<br> cmip5_tp_energy_transport.nc (the third column of figure 17 in appendix)<br> </p>
Optical parametric amplification seeded by four-wave mixing in photonic crystal fibres
<p>Open access dataset for figures in 'Optical parametric amplification seeded by four-wave mixing in photonic crystal fibres', accepted for publication in Nonlinear Frequency Generation and Conversion: Materials and Devices XXI, paper 11985-4, SPIE LASE Photonics West, 2022.</p>
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.