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66 results for “biosecurity”
CS3 Experiment 7 Abalone growth in a Land Based IMTA system on commercial feeds incorporating biosecure IMTA grown algae
<p>CS3 Experiment 7 Abalone growth in a Land Based IMTA system on commercial feeds incorporating biosecure IMTA grown algae</p>
Data from: RapidRat: development, validation and application of a genotyping-by-sequencing panel for rapid biosecurity and invasive species management
Open the record for dataset details and reuse information.
Supplementary material 2 from: McNeill MR, Phillips CB, Richards NK, Aalders LT, van Koten C, James TK, Young SD, Bell NL, Laugraud A (2023) Defining the biosecurity risk posed by soil found on sea freight. NeoBiota 88: 103-133. https://doi.org/10.3897/neobiota.88.98440
Soil contamination data, Insect taxa, Pseudomonas isolates and reference species, and plant taxa identified by molecular analysis
Supplementary material 1 from: McNeill MR, Phillips CB, Richards NK, Aalders LT, van Koten C, James TK, Young SD, Bell NL, Laugraud A (2023) Defining the biosecurity risk posed by soil found on sea freight. NeoBiota 88: 103-133. https://doi.org/10.3897/neobiota.88.98440
Sampling points on sea container and visualisation of the genetic diversity between the 16S rRNA genes
Detection of Khapra Beetle environmental DNA using portable technologies in Australian biosecurity
<p>Khapra beetle, Trogoderma granarium Everts, 1898, is a serious pest of stored grain products globally. Environmental DNA (eDNA)-based methods offer sensitive detection tools used to inform biosecurity officers on the presence of high-risk pests. This study tested laboratory and portable molecular technologies to detect khapra beetle environmental DNA extracted from dust samples collected during biosecurity responses (Tuggeranong and Fyshwick) to khapra beetle incursions in Australia. Airborne and floor dust samples were collected opportunistically using handheld vacuum cleaners and eDNA was extracted using either field or laboratory-based extraction methods and analyzed using laboratory benchtop real time PCR machines and portable machines with two TaqMan and one LAMP-based assay. We successfully collected, extracted, and amplified khapra beetle eDNA from dust samples by qPCR, but failed to amplify T. granarium eDNA using LAMP. The Laboratory qPCR machine showed significantly higher mean Ct values (p &lt; 0.001) and significantly higher positive detections for both assays (p &lt; 0.001) compared to the portable thermocycler. DNA yield was significantly higher in samples extracted using laboratory-based kits compared to field kits (p &lt; 0.001) for both vacuumed and airborne samples (Mean DNA ± S.D. = 5.52 ± 4.45 and 4.77 ± 1.68 ng/μL, respectively), compared to field kits, (1.75 ± 1.17 and 1.36± 1.29 ng/μL for vacuumed and airborne samples, respectively). There were no significant differences in DNA yield between collection methods or differences in amplification associated to extraction or collection methods in either platform tested in this study. Portable technologies tested in this study (Franklin™ Real Time Thermocycler and Genie III) accurately amplified all tissue derived DNA during assay optimisation and field testing, highlighting the capacity of these technologies to complement biosecurity in confirming specimen ID. There was a high incidence of positive detections in field negative controls (Tuggeranong = 12.3 % and Fyshwick = 50 %), mostly attributed to the use of contaminated vacuum cleaners. We discuss suitable methods to minimize sample cross-contamination, the potential of portable molecular technologies as tools for biosecurity applications, and the suitability of eDNA-based molecular detection methods to complement global trade biosecurity for one of the most invasive and important grain pests worldwide.</p>
FIGURE 18. Hyperlasion aliens Mohrig, 2004 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 18. Hyperlasion aliens Mohrig, 2004 (specimen from Papua New Guinea). A. Hypopygium. B. Flagellomere 3–5. C. Male.
FIGURE 17 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 17. Scatopsciara atomaria (Zetterstedt, 1851). A. Hypopygium. B. Flagellomeres 4–6. C. Palpus. D. Fore tibia.
FIGURE 16 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 16. Bradysia strenua (winnertz, 1867). A. Left side of the hypopygium in ventral view. B. Gonostylus. C. 4th flagellomere. D. Scutellum.
FIGURE 15 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 15. Corynoptera concinna (winnertz, 1867). A. Hypopygium. B. Basal segments of antenna. C. Fore tibia.
FIGURE 14 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 14. Bradysia spatitergum (Hardy, 1956). A. Hypopygium, ventral side. B. Hypopygium, dorsal side. C. Flagellomeres 3–5.
FIGURE 13 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 13. Bradysia pallipes (Fabricius, 1787); (specimen from Australia). A. Hypopygium (left half in ventral view).
FIGURE 11 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 11. Pnyxia scabiei (Hopkins, 1895). A. Hypopygium. B. Head with basal segments of antenna (male). C. Fore tibia.
FIGURE 9 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 9. Cosmosciara hartii (Johannsen, 1912). (A, C and D, morphotype II). A. Head, female (specimen from Hawaii). B. Head, female (morphotype I, from Europe). C. Male (specimen from Fiji). D. Head and thorax of female (specimen from Hawaii).
FIGURE 10 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 10. Bradysia tilicola (Loew, 1850); (specimen from Europe). A. Hypopygium. B. Flagellomeres 4–5. C. Palpus. D. Fore tibia. E. wing.
FIGURE 5 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 5. Lycoriella ingenua (Dufour, 1839). A. Hypopygium. B. Gonostylus. C. Flagellomeres 2–4. D. Palpus. E. Fore tibia.
FIGURE 8 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 8. Cosmosciara hartii (Johannsen, 1912). Male (morphotype I). A. Hypopygium. B. Distal part of gonostylus. C. Flagellomeres 2–6.
FIGURE 4 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 4. Lycoriella agraria (Felt, 1898). A. Hypopygium. B. Basal segments of antenna. C. Palpus. D. Fore tibia. E. wing.
FIGURE 1 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 1. Bradysia impatiens (Johannsen, 1912). Form A (typical pest specimen). A. Hypopygium. B. Flagellomeres 3–5. C. Palpus. D. Fore tibia. E. wing.
FIGURE 3 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 3. Bradysia ocellaris (Comstock, 1882). A. Hypopygium. B. Head and thorax. C. Basal segments of antenna. D. Palpus. E. Fore tibia.
FIGURE 2 in Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions
FIGURE 2. Bradysia impatiens (Johannsen, 1912). Form B. (specimen from Kingston, Tasmania, glasshouse). A. Hypopygium. B. Gonostylus. C. Flagellomeres 3–4.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.