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106 results for “cell length”
Purification of full-length huntingtin Q23 from insect cells - 25th January 2018
<p>Huntingtin structure-function open lab notebook project</p>
Binding From both sides: TolR and full-length OmpA bind and maintain the local structure of the E. coli cell wall.
<p>We present a molecular modelling and simulation study of the <em>E. coli </em>cell envelope, with a particular focus on the role of TolR, a native protein of the <em>E. coli </em>inner membrane in interactions with the cell wall. TolR has been proposed to bind to peptidoglycan, but the only structure of this protein thus far is in a conformation in which the putative peptidoglycan binding domain is not accessible. We show that a model of the extended conformation of the protein in which this domain is exposed, binds peptidoglycan largely through electrostatic interactions. Non-covalent interactions of TolR and OmpA with the cell wall, from the inner membrane and outer membrane sides respectively, maintain the position of the cell wall even in the absence of Braun’s lipoprotein. The charged residues that mediate the cell-wall interactions of TolR in our simulations, are conserved across a number of species of Gram-negative bacteria.</p>
Impact on the Length of Stay in Incentive Spirometry and Pain in the Decompensation of Sickle Cell Disease: .
ClinicalTrials.gov study NCT04041180. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Telomere Length in Human Polar Body and Telomere Length in Cumulus Cells: A Clinical Validation Study
ClinicalTrials.gov study NCT04577560. IPD Sharing: YES. Countries: 1. Publications: 11.
Does Recombinant FSH (rFSH, i.g. Gonal F®) as Compared to Human Menopausal Gonadotrophin (hMG) Affect Telomere Length of Cumulus Cells During Antral Follicle Growth and Impact Blastocyst Status?
ClinicalTrials.gov study NCT06732843. IPD Sharing: UNDECIDED. Countries: 1. Publications: 15.
Data from: Threshold effect of growth rate on population variability of Escherichia coli cell lengths
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Epidermal cell length of <em>Bursera</em> <em>simaruba</em> and <em>Eucalyptus camaldulensis</em> leaves
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Systematic profiling of full-length immunoglobulin and T-cell receptor repertoire diversity in rhesus macaque through long read transcriptome sequencing
<p>Using long read sequencing, we sequenced four Indian-origin rhesus macaque tissues. From raw full-length, non-chimeric circular consensus sequencing (CCS) reads, we obtained high quality, full-length sequences for over 6,000 unique immunoglobulin and T-cell receptor transcripts, without the need for sequence assembly.</p>
Data from: Systematic study of the surface plasmon resonance signals generated by cells for sensors with different characteristic lengths
The objectives of this study were to establish an in-depth understanding of the signals induced by mammalian cells in surface plasmon resonance (SPR) sensing. To this end, two plasmonic structures with different propagation and penetration distances were used: conventional surface plasmon resonance and long-range surface plasmon resonance. Long-range SPR showed a lesser sensitivity to the absolute number of round cells but a greater resolution due to its very narrow spectral dip. The effect of cell spreading was also investigated and the resonance angle of long-range SPR was mostly insensitive unlike in the conventional SPR counterpart. Experimental data was compared with suitable models used in the SPR literature. Although these simple averaging models could be used to describe some of the experimental data, important deviations were observed which could be related to the fact that they do not take into consideration critical parameters such as plasmon scattering losses, which is particularly crucial in the case of long-range SPR structures. The comparison between conventional and long-range SPR for cellular schemes revealed important fundamental differences in their responses to the presence of cells, opening new horizons for SPR-based cell assays. From this study, long-range SPR is expected to be more sensitive towards both the detection of intracellular events resulting from biological stimulation and the detection of microorganisms captured from complex biological samples.
Influence of alkyl chain length on the photovoltaic properties of dithienopyran-based hole-transporting materials for perovskite solar cells
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Exploring the cellular landscape of circular RNAs using full-length single-cell RNA sequencing
<p>Source data for our circSC manuscript.</p>
Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/08/04
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Rationale </strong>- Purified huntingtin samples with and without stabilising binding partners (HAP40) are required for use in structural and functional studies, in particular SAXS experiments. </p>
Text-fig. 34. Synchrotron radiation X-ray tomographic microscopy (SRXTM, a–h) images of "Paisia-like follicle"; Catefica locality, Portugal. a–d) Volume rendering of follicles in lateral (a, c) and ventral (b, d) views showing the decurrent stigmatic region that extends from base of the follicle to the apex; note papillate zone forming a probable stigma along the full length of the ventral suture; e) Longitudinal section (volume rendering cut at orthoslice yz0326) near the base of the follicle showing two ovules with a striate-reticulate surface (asterisks); note transverse fibers lining the inner follicle wall and large cells of the mesocarp; f) Transverse section (volume rendering cut at orthoslice xy2475) of follicle showing two rows of ovules borne on placentae on either side of the ventral suture (asterisks); note also the pronounced and densely-spaced papillae around the ventral suture; g) Transverse section (orthoslice xy1988) of follicle showing two ventral vascular bundles and one dorsal bundle (arrows) and ovules/seeds borne on two placentae, one on either side of the ventral suture; note the remains of the small thin-walled cells of the outer epidermis that cover the thicker-walled cells of the mesocarp; h) Transverse section (orthoslice xy2860) of follicle showing two ventral bundles and one dorsal bundle (arrows) and ovules/seeds in two rows on the placentae, one on either side of the ventral suture; note the remains of small epidermal cells and the large rounded cells of the mesocarp with thicker walls. Specimens, Catefica 49-S174916 (a, b), Catefica 49-S174917 (c–f, h), Catefica 50-S171525 (g). Scale bars = 300 Μm (a–d), 100 Μm (e–h). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 34. Synchrotron radiation X-ray tomographic microscopy (SRXTM, a–h) images of "Paisia-like follicle"; Catefica locality, Portugal. a–d) Volume rendering of follicles in lateral (a, c) and ventral (b, d) views showing the decurrent stigmatic region that extends from base of the follicle to the apex; note papillate zone forming a probable stigma along the full length of the ventral suture; e) Longitudinal section (volume rendering cut at orthoslice yz0326) near the base of the follicle showing two ovules with a striate-reticulate surface (asterisks); note transverse fibers lining the inner follicle wall and large cells of the mesocarp; f) Transverse section (volume rendering cut at orthoslice xy2475) of follicle showing two rows of ovules borne on placentae on either side of the ventral suture (asterisks); note also the pronounced and densely-spaced papillae around the ventral suture; g) Transverse section (orthoslice xy1988) of follicle showing two ventral vascular bundles and one dorsal bundle (arrows) and ovules/seeds borne on two placentae, one on either side of the ventral suture; note the remains of the small thin-walled cells of the outer epidermis that cover the thicker-walled cells of the mesocarp; h) Transverse section (orthoslice xy2860) of follicle showing two ventral bundles and one dorsal bundle (arrows) and ovules/seeds in two rows on the placentae, one on either side of the ventral suture; note the remains of small epidermal cells and the large rounded cells of the mesocarp with thicker walls. Specimens, Catefica 49-S174916 (a, b), Catefica 49-S174917 (c–f, h), Catefica 50-S171525 (g). Scale bars = 300 Μm (a–d), 100 Μm (e–h).
Measurment of Optic Disc Parameters, RNFL Thickness and Ganglion Cell Complex in Myopic, Hypermetropic and Emmetropic Patients Using OCT and Axial Length Measurments
ClinicalTrials.gov study NCT07241806. IPD Sharing: UNDECIDED. Countries: 0. Publications: 1.
Data from: Systematic study of the surface plasmon resonance signals generated by cells for sensors with different characteristic lengths
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Data from: Age-related declines in immune response in a wild mammal are unrelated to immune cell telomere length
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Double-stranded microRNA mimics can induce length- and passenger strand-dependent effects in a cell type-specific manner (RNA 2015), Exp 1
GEO Series GSE75800. Homo sapiens. 12 samples. Type: Expression profiling by array.
Genome-wide DNA replication profiling and full-length total RNA sequencing from the same single cell [01_RPE1_midS]
GEO Series GSE278941. Homo sapiens. 77 samples. Type: Expression profiling by high throughput sequencing; Other.
Simultaneous profiling of full-length RNA transcripts and chromatin accessibility within single cells of human retinal organoids
GEO Series GSE251754. Homo sapiens. 10 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing.
Genome-wide DNA replication profiling and full-length total RNA sequencing from the same single cell [03_CBMS1_mESC]
GEO Series GSE278949. Mus musculus. 168 samples. Type: Expression profiling by high throughput sequencing; Other.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.