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Summer water chemistry; sediment phosphorus fluxes and sorption capacity; sedimentation and sediment resuspension dynamics; water column thermal structure; and zooplankton, macroinvertebrate, and macrophyte communities in eight shallow lakes in northwest Iowa, USA (2018-2020)
The primary aim of this data product is to characterize change in water chemistry, sediment-water interactions, and biological communities in shallow, eutrophic lakes undergoing a fishery biomanipulation. We studied eight glacial lakes located in northwest Iowa, USA, from 2018 to 2020 during the summer season (May to September). A subset of these lakes (n = 4; Center, Five Island, North Twin, and Silver Lakes) were part of a fishery biomanipulation in which the Iowa Department of Natural Resources (IDNR) incentivized commercial harvest of common carp (Cyprinus carpio) and bigmouth buffalo (Ictiobus cyprinellus). Harvests occurred in Center and Five Island Lakes during 2018-2019 and in North Twin and Silver Lakes during 2019-2020. Between 73 and 373 kg fish biomass per ha were removed each year. The other study lakes (n = 4; Blue, South Twin, Storm, and Swan Lakes) remained unmanipulated during the study period. Over the course of the biomanipulation, we quantified a suite of physical, chemical, and biological parameters across the study lakes. High frequency aquatic sensors were used to measure water column thermal structure, dissolved oxygen concentrations, and algal pigments. Manual water chemistry sampling further quantified suspended solids, total phosphorus and nitrogen, soluble reactive phosphorus, nitrate, and water clarity. We measured flux rates of phosphorus between bottom sediments and the overlying water using ex situ sediment core incubations under both oxic and anoxic conditions. We further quantified sediment phosphorus sorption capacity using equilibrium phosphorus concentration assays. Tiered sediment traps were used to measure sedimentation rates as well as sediment resuspension in bottom waters. We also measured change in zooplankton, macroinvertebrate, and macrophyte community composition and abundance. These data will be used to better understand the mechanisms of internal phosphorus loading in shallow lakes and the ecosystem effects of fisherie
Dynamic landscapes of fear and safety alter prey refuge use in freshwater habitats
The non-consumptive effects of predators on prey behavior have been studied in many different systems. However, predator-prey ecology has placed a bulk of emphasis on how fear alters prey behavior, and new studies have begun to shift focus to the importance that safety in the form of refuges has in structuring prey behavioral responses. This project focuses on changes in the safety landscape as well as changes in the fear landscape and how these changes impact crayfish behavior. Using an established bass-crayfish predator prey system, we altered shelter quality and location in relation to the presence of bass odor signals. We measured shelter use by the crayfish in response to this changing landscape.
MCR LTER: Coral Reef: Long-term Population and Community Dynamics: Other Benthic Invertebrates, ongoing since 2005 (Reformatted to the ecocomDP Design Pattern)
This data package is formatted as an ecocomDP (Ecological Community Data Pattern). For more information on ecocomDP see https://github.com/EDIorg/ecocomDP. This Level 1 data package was derived from the Level 0 data package found here: https://pasta.lternet.edu/package/metadata/eml/knb-lter-mcr/7/32. The abstract below was extracted from the Level 0 data package and is included for context: The data presented here are the abundances of the major invertebrate herbivores and corallivores on Moorea coral reefs. Abundances are estimated in 4 fixed quadrats along 5 permanent transects at each of 4 habitats at 2 sites on each of the 3 shores of Moorea each year. Counts are made in one-meter-squared quadrats. This material is based upon work supported by the U.S. National Science Foundation under Grant No. OCE 16-37396 (and earlier awards) as well as a generous gift from the Gordon and Betty Moore Foundation. Research was completed under permits issued by the French Polynesian Government (Délégation à la Recherche) and the Haut-commissariat de la République en Polynésie Francaise (DTRT) (Protocole d'Accueil 2005-2020). This work represents a contribution of the Moorea Coral Reef (MCR) LTER Site.
Seedling composition, growth, and dynamics in tropical rain forest, La Selva, Costa Rica (1983-1996)
Recruitment, growth, and survivorship of the regeneration stages of trees and lianas were studied in old-growth tropical rain forest at La Selva Biological Station of the Organization for Tropical Studies (OTS), near Puerto Viejo de Sarapiquí, Heredia Province, in the Caribbean lowlands of Costa Rica. A total of 48 permanent seedling transects each measuring 10 m x 0.5 m were established at random locations within three La Selva permanent forest inventory plots. The forest plots occupy contrasting landforms: Plot 1 (4.4 ha), old alluvial terrace; Plot 2 (4.0 ha), swamp forest and low hills; and Plot 3 (4.0 ha), steeply dissected terrain with residual volcanic soils. Seedling locations are georeferenced within the grid system of the permanent forest inventory plots, facilitating spatial analysis of seedling populations with respect to adult cohorts. Beginning in June 1983, all seedlings ≤ 0.5 m in height belonging to tree and liana species capable of reaching 10 cm diameter at breast height (dbh) at maturity were tagged, identified to species or morphospecies, mapped to the nearest cm, and measured in height to the nearest cm. Over a period of 18 months, a total of 6403 seedlings belonging to 167 species were tagged. Monitoring and re-measurement of all tagged individuals continued through November 1996. Data include 17 census dates over a period of 13.5 years. At the time of the final census, only 97 individuals (1.52% of the tagged seedlings) were still alive, representing 43 species (25.7% of the initial number). The largest surviving seedling had grown in height from 4 cm to 13 meters during the study period. This dataset on the regeneration stages in old-growth tropical rain forest in the La Selva permanent inventory plots forms a complement to the studies of long-term growth and demography of these species and assemblages at adult stages within the plots. Forest inventory data for trees and lianas ≥ 10 cm dbh in the permanent plots in which the seedling transec
Altering pH changes competition dynamics between two crayfish species for both food and shelter resources
As climate change continues, alterations in abiotic variables within habitats will also change. One of the key variables for aquatic systems is pH. We were interested in how lowered pH altered the behavioral characteristics of two keystone species: Faxonius rusticus, a non-native crayfish in the upper midwest and Faxonius virilis, the native crayfish. We dosed size-matched individuals in separate tanks for four days and than placed these individuals in a flow through mesocosm to allow them to compete over food and shelter resources. Behavior was recorded from midnight to 4 am. Pairs of animals were exposed to pH levels varying from ambient (8.4) to 6.4. The experiment took place at the University of Michigan's Biological Station in northern Michigan in the United States.
Impacts of microplastics on wetland ecosystem dynamics: a mesocosm study of trophic interactions and community responses, 2021-2025
This dataset documents a mesocosm experiment conducted to evaluate the ecological impacts of microplastics (MP) on wetland communities representative of eastern USA wetlands. The study focused on key organisms across trophic levels, including tadpoles (Lithobates pipiens), snails (Helisoma trivolvis), zooplankton (Daphnia pulex), phytoplankton, and periphyton communities, to assess the effects of three microplastic types (low-density polyethylene–LDPE, medium-density polystyrene–PS, and high-density polyester–PES) at two concentrations (1 mg/L and 5 mg/L), alongside a no-microplastic control. Experimental units consisted of 70 mesocosms (19-L buckets) with 10 replicates per treatment, established between July 1–15, 2021, at Binghamton University’s Ecological Research Facility. Response variables included survival and developmental traits (mass, snout-vent length, shell width) of tadpoles and snails, microplastic ingestion, zooplankton abundance, phytoplankton biomass (chlorophyll and phycocyanin concentrations), and periphyton mass. The dataset provides comprehensive measurements of community responses and water quality parameters, offering insights into the ecological consequences of microplastic pollution in wetland ecosystems. This dataset is suitable for researchers studying ecotoxicology, wetland ecology, and the impacts of anthropogenic pollutants on aquatic food webs.
Data associated with the FLooded Upland Dynamics EXperiment (FLUDEX), conducted at the IISD Experimental Lakes Area 1997 to 2003, investigating reservoir flooding impacts on ecosystems, particularly the release of mercury and greenhouse gases.
The data included in this repository were collected over the course of the FLooded Upland Dynamics Experiment (FLUDEX) conducted at the IISD Experimental Lakes Area (IISD-ELA) from 1997 to 2003. A plethora of data was collected over five years of flooding three upland reservoir sites, in order to examine the relationship between the amount of flooded, and thus decomposed, terrestrial organic matter and the production of methylmercury (MeHg), total mercury (THg), and greenhouse gases (GHGs) in the reservoirs. Findings from this experiment suggest that the amount of organic carbon stored in a flooded site does not directly influence the amount of THg, MeHg, and GHGs produced, but it does affect the persistence of mercury in the reservoir and food web. This version of the repository contains data collected on water chemistry, benthic invertebrate (chironomid) emergence, mercury and methylmercury concentrations in the water and food web, stable isotopes of carbon and nitrogen in emerging insects and zooplankton, and abundance and biomass of zooplankton, phytoplankton, and bacteria. This data package contains only some of the data from the FLUDEX project. IISD-ELA hopes to add more data in subsequent versions.
MCR LTER: Coral Reef: Long-term Population and Community Dynamics: Corals, ongoing since 2005 (Reformatted to the ecocomDP Design Pattern)
This data package is formatted as an ecocomDP (Ecological Community Data Pattern). For more information on ecocomDP see https://github.com/EDIorg/ecocomDP. This Level 1 data package was derived from the Level 0 data package found here: https://pasta.lternet.edu/package/metadata/eml/knb-lter-mcr/4/38. The abstract below was extracted from the Level 0 data package and is included for context: This dataset contains the percentage cover of the stony corals (Scleractinia) and other major groups analyzed from 0.5 x 0.5 m photographic quadrats in several reef habitats at the Moorea Coral Reef LTER, French Polynesia. This survey has been repeated annually in April since 2005. There are two tables available, providing different views of the same data: a long table having all values in one column and a wide table having a separate column for each dependent variable. Functional groups (i.e., dependent variables) counted are: Scleractinian Corals (by genus where appropriate, see methods), Macroalgae, Crustose Coralline Algae / Bare Space, Soft Corals, Hydrocorals (Millepora), Algal Turf and Sand. The coral community was sampled photographically in all habitats surrounding the island: Fringing Reef, Lagoon (Backreef), and Outer Reef (Forereef.) The sampling regime consists of a repeated-measures protocol in each habitat, and is structured by habitat to allow a statistical contrast of sites, shores, times, and in the case of the outer reef, depths. Detailed methods are available in the protocols section. This material is based upon work supported by the U.S. National Science Foundation under Grant No. OCE 16-37396 (and earlier awards) as well as a generous gift from the Gordon and Betty Moore Foundation. Research was completed under permits issued by the French Polynesian Government (Délégation à la Recherche) and the Haut-commissariat de la République en Polynésie Francaise (DTRT) (Protocole d'Accueil 2005-2020). This work represents a contribution of the Moorea Coral Reef (MCR)
MCR LTER: Coral Reef: Long-term Population and Community Dynamics: Benthic Algae and Other Community Components, ongoing since 2005 (Reformatted to the ecocomDP Design Pattern)
This data package is formatted as an ecocomDP (Ecological Community Data Pattern). For more information on ecocomDP see https://github.com/EDIorg/ecocomDP. This Level 1 data package was derived from the Level 0 data package found here: https://pasta.lternet.edu/package/metadata/eml/knb-lter-mcr/8/32. The abstract below was extracted from the Level 0 data package and is included for context: Coral reefs are comprised of scleractinian corals and many other benthic organims. The sampling described here quantifies the relative abundances of corals (aggregate abundance) and the other major benthic components including algal turfs, macroalgae, crustose corallines, and other sessile invertebrates. Abundance is estimated yearly at each of 6 sites (2 per shore) around the island. At each site, and in each of 4 habitats (fringing reef, backreef, forereef 10-m depth, forereef 17-m depth), 5 permanent 10-m long transects have been established and abundance estimates are made at fixed positions along each transect (n=10, 0.25 m2 quadrats per transect) allowing a repeated measures statistical analysis for the detection of temporal trends. This material is based upon work supported by the U.S. National Science Foundation under Grant No. OCE 16-37396 (and earlier awards) as well as a generous gift from the Gordon and Betty Moore Foundation. Research was completed under permits issued by the French Polynesian Government (Délégation à la Recherche) and the Haut-commissariat de la République en Polynésie Francaise (DTRT) (Protocole d'Accueil 2005-2020). This work represents a contribution of the Moorea Coral Reef (MCR) LTER Site.
MCR LTER: Coral Reef: Long-term Community Dynamics: Backreef (Lagoon) Corals Annual Survey, ongoing since 2005 (Reformatted to the ecocomDP Design Pattern)
This data package is formatted as an ecocomDP (Ecological Community Data Pattern). For more information on ecocomDP see https://github.com/EDIorg/ecocomDP. This Level 1 data package was derived from the Level 0 data package found here: https://pasta.lternet.edu/package/metadata/eml/knb-lter-mcr/1038/10. The abstract below was extracted from the Level 0 data package and is included for context: This dataset contains the percentage cover of all stony corals (Scleractinia, pooled among genera) and other major groups analyzed from 0.5 x 0.5 m photographic quadrats at the Backreef habitat at the Moorea Coral Reef LTER, French Polynesia. This survey time series began in 2005 and is repeated each year in April. Functional groups counted are: Scleractinian corals, Macroalgae, Crustose Coralline Algae / Bare Space, Soft Corals, Hydrocorals (Millepora), Algal Turf and Sand. The coral community was sampled photographically in all represented habitats surrounding the island: Fringing Reef, Lagoon, and Outer Reef. This dataset contains only Lagoon (Backreef) data (see knb-lter-mcr.4 for the other habitats) and is structured in a repeated-measures protocol to allow a statistical contrast of sites, shores and times. Community structure was determined through a coarse analysis of the benthic community, initially completed in situ (2005), but using photoquadrats from 2006. There are quadrats analyzed at each of five areas within each site, and the areas are revisited (but not the quadrats) each year to support the repeated measures design. There are two tables available, providing different views of the same data: a long table having all values in one column and a wide table having a separate column for each observed object. Detailed methods are available in the protocols section. This material is based upon work supported by the U.S. National Science Foundation under Grant No. OCE 16-37396 (and earlier awards) as well as a generous gift from the Gordon and Betty Moore Foundation. R
MCR LTER: Coral Reef: Long-term Population and Community Dynamics: Other Benthic Invertebrates, ongoing since 2005 (Reformatted to a Darwin Core Archive)
This data package is formatted as a Darwin Core Archive (DwC-A, event core). For more information on Darwin Core see https://www.tdwg.org/standards/dwc/. This Level 2 data package was derived from the Level 1 data package found here: https://pasta.lternet.edu/package/metadata/eml/edi/194/3, which was derived from the Level 0 data package found here: https://pasta.lternet.edu/package/metadata/eml/knb-lter-mcr/7/32. The abstract below was extracted from the Level 0 data package and is included for context: The data presented here are the abundances of the major invertebrate herbivores and corallivores on Moorea coral reefs. Abundances are estimated in 4 fixed quadrats along 5 permanent transects at each of 4 habitats at 2 sites on each of the 3 shores of Moorea each year. Counts are made in one-meter-squared quadrats. This material is based upon work supported by the U.S. National Science Foundation under Grant No. OCE 16-37396 (and earlier awards) as well as a generous gift from the Gordon and Betty Moore Foundation. Research was completed under permits issued by the French Polynesian Government (Délégation à la Recherche) and the Haut-commissariat de la République en Polynésie Francaise (DTRT) (Protocole d'Accueil 2005-2020). This work represents a contribution of the Moorea Coral Reef (MCR) LTER Site.
Dynamics of montane and subalpine meadows in the Three Sisters Wilderness Area and Biosphere Reserve, 1981-1993
The Three Sisters Wilderness Area is a Biosphere Reserve that represents the northern half of the Sierra-Cascade Province. It encompasses nearly 100,000 ha of essentially undisturbed landscape straddling the crest of the central Cascade Range, Oregon. Coniferous forests dominate the Three Sisters, although the Wilderness also supports a diversity of non-forested habitats including montane and subalpine meadows. The Three Sisters was given wilderness status in 1957 and was selected as a UNESCO Biosphere Reserve in 1974, to represent a "control" area for the nearby H. J. Andrews Biosphere Reserve. Integral to the establishment of a Biosphere Reserve is the documentation of existing ecosystems with baseline data. However, basic ecological research in theThree Sisters has been relatively limited. We conducted two types of studies: (1) phytosociological studies to characterize current vegetation patterns and to interpret relationships between community composition and major environmental and edaphic gradients, and (2) retrospective analyses of the invasion of montane and subalpine meadows by trees with a focus on the effects of recent climatic variation, grazing history, and site environment.
History and Dynamics of American Beech in Coastal New England 1620-2006
Variation in forest response to hurricane disturbance in coastal New England Research on disturbance in forest ecosystems has generally focused on either catastrophic disturbances generating stand-replacing successional sequences, or small-scale disturbances (e.g. individual tree-fall gaps) resulting in tree-by-tree replacement. The role of moderate disturbances in forest development, in contrast, is poorly understood. While the most severe hurricanes can cause catastrophic disturbance, the vast majority of hurricanes that affect forests from the Caribbean to the northeastern United States are moderate in intensity. In this study we examine both individual and population level responses of beech (Fagus grandifolia) and oak species (Quercus spp.) to hurricanes of varying intensity in coastal Massachusetts. We characterize growth response to disturbance using a novel approach that explicitly compares the range of growth responses observed following known disturbances to the range of growth responses in non-event years. The tree species exhibited a wide range of growth and regeneration responses to hurricanes; however, only a single storm caused dramatic increases in growth and new establishment for beech. The results of this study highlight the importance of wind disturbance in the establishment and persistence of beech, and suggest that while some moderate disturbances have little or no effect on species growth and regeneration dynamics, individual storms may have substantial impacts. Forest response to wind storms of varying, but moderate intensities, depended on local site conditions, including environmental, meteorological, topographical, historical and biological factors. Beech dominance in a coastal New England forest Monodominant forests occur in a wide range of tropical and temperate ecosystems, but the mechanisms enabling their development are not well understood. This study examines the history and dynamics of beech-dominated forests in coastal New England i
Long-term Dynamics of Vegetation and Environment in Central Massachusetts 1000-2000
Historical and ecological data from north-central Massachusetts suggest that widespread and intensive human disturbance after European settlement led to a shift in forest composition and obscured regional patterns of species abundance. A paleoecological approach was required to place recent forest dynamics in a long-term context. Pollen and charcoal data from 11 small lakes in north-central Massachusetts were used to reconstruct local vegetation dynamics and fire histories across the region over the past 1000 years. The sites are located across an environmental gradient. Paleoecological data indicate that prior to European settlement, there was regional variation in forest composition corresponding to differences in climate, substrate, and fire regime. Oak, chestnut and hickory were abundant at low elevations, whereas hemlock, beech, sugar maple, and yellow birch were common at high elevations. Fire appears to have been more frequent and/or intense at lower elevations, maintaining high abundances of oak, and archaeological data suggest Native American populations were greater in these areas. A change in forest composition at higher elevations, around 550 years before present, may be related to the Little Ice Age (a period of variable climate), fire, and/or activity by Native Americans, and led to regional convergence in forest composition. After European settlement, forest composition changed markedly in response to human disturbance and there was a sharp increase in rates of vegetation change. Regional patterns were obscured further, leading to homogenization of broad-scale forest composition. There is no indication from the pollen data that forests are returning to pre-European settlement forest composition, and rates of vegetation change remain high, reflecting continuing disturbance to the landscape, despite regional reforestation.
Long-term Stand Dynamics in Central Massachusetts 1500-2000
The long-term impact of human land-use is among the most important factors influencing the development of vegetation in New England, a region which experienced extensive agricultural clearance in the 19th century and subsequent reforestation during this century. We employed stand-level pollen stratigraphies and tree-ring chronologies to examine the post-settlement dynamics of hemlock stands which have never been pastured or plowed, representing among the least disturbed parts of the central Massachusetts landscape. Although the sites are currently dominated by large Tsuga canadensis individuals and give the impression of great age and stability, our results indicate that they have dynamic developmental histories driven by exogenous disturbance factors, including logging, forest pathogens, catastrophic wind disturbance, and fire. Sites containing pre-settlement pollen assemblages indicate stand compositions at this time which were substantially different from each other and from modern assemblages. Pollen assemblages similar to those of modern stands were not established in any of our sites until the 20th century and the mechanisms by which these assemblages arose was considerably different in each stand, indicating great flexibility of forest response to a variety of disturbance types.
Linking Community Dynamics and Ecosystem Function at Harvard Forest 1996-2000
Human activities are effecting profound changes in the structure and function of natural ecosystems. A comprehensive understanding of current ecosystem dynamics and future responses to global change requires an integrated investigation of ecological processes at many levels of organization. My thesis research addressed this goal by examining interactions between community- and ecosystem-level dynamics in mixed conifer broad-leaved forests in eastern North America. I addressed the nature of canopy-seedling feedbacks in mixed forests by relating seedling regeneration patterns in contrasting stand types to understory conditions (EXPERIMENT 1), and by directly manipulating resource availability to separate the individual effects of particular resources (EXPERIMENT 2). To investigate how nitrogen deposition will influence future forest composition, I examined the impact of increased nitrogen availability on regeneration of both coniferous and broad-leaved tree species under both closed canopy (EXPERIMENT 3) and simulated gap (EXPERIMENT 4) conditions. Future changes in forest composition might then influence whole-ecosystem productivity. I used two scaling approaches (leaf-level aggregation, EXPERIMENT 5; whole-tree sap flow, EXPERIMENT 6), I examined how the dominant coniferous and broad-leaved species in mixed temperate forests differed in their contributions to canopy-level photosynthesis.
Long-term Vegetation Dynamics on the Massachusetts Coast from 2000 BP to Present
We use a retrospective approach to reconstruct the past distribution of fire in New England and to investigate the important drivers of this pattern across the period of European arrival to North America. Our study sites are in New England, and range from pitch pine and oak forests of coastal Massachusetts, pine and hardwood forests of central Massachusetts, and northern hardwood and spruce fir forests of northern Massachusetts and Vermont. We collected sediment profiles from 18 lakes across the study area to assess fossil charcoal and pollen abundance over the past 1000 years and including the time period of European arrival and settlement. Based on presettlement pollen composition, our study sites are divided into three vegetation types: 1) pitch pine and oak, 2) oak, pine, and hardwood, and 3) northern hardwoods. The abundance of presettlement charcoal in these lakes is closely related to climate and the composition of surrounding vegetation. Charcoal is most abundant in pitch pine forests and least common in northern hardwood and spruce forests. Following the arrival of Europeans, charcoal abundance increases, at most sites substantially, and vegetation composition changed in a direction of either greater dominance by pitch pine or white pine, depending on whether the forests were located in the southern or northern part of New England. The major factor influencing the distribution of fire across New England is climate, which has a direct effect on the physical conditions conducive to fire ignition and spread and an indirect effect on fire through its control on the distribution of vegetation at this spatial scale. We find evidence that other factors exert some control over local fire regimes as well including landforms and their impact on vegetation composition, firebreaks, and prevailing winds. Native Americans likely influenced the local occurrence of fire, but their impact on regional fire regimes in New England is not apparent from this or other studies. Ho
Long-term Vegetation Dynamics in Southwestern New Hampshire from 13000 BP to Present
The paleoecological record allows contemporary ecologists to put current phenomena into the context of a longer time-frame, thereby providing the opportunity to evaluate the importance of slowly operating processes, past cyclic or unusual events, disturbance regimes, and historically constrained phenomena. We briefly outline the environmental history of the Quaternary, discuss the spatial and temporal resolution of the paleoecological evidence for biotic change, and summarize data relevant to such current issues as the nature of the biotic community, the role of disturbance, stability versus rapid change, evolutionary theory, explanations of species diversity, and refugia theory. Finally, we offer examples of the utility of paleoecological techniques for ecologists and environmental scientists.
Microbial Observatory at North Temperate Lakes LTER Time series of bacterial community dynamics in Lake Mendota 2000 - 2009
With an unprecedented decade-long time series from a temperate eutrophic lake, we analyzed bacterial and environmental co-occurrence networks to gain insight into seasonal dynamics at the community level. We found that (1) bacterial co-occurrence networks were non-random, (2) season explained the network complexity and (3) co-occurrence network complexity was negatively correlated with the underlying community diversity across different seasons. Network complexity was not related to the variance of associated environmental factors. Temperature and productivity may drive changes in diversity across seasons in temperate aquatic systems, much as they control diversity across latitude. While the implications of bacterioplankton network structure on ecosystem function are still largely unknown, network analysis, in conjunction with traditional multivariate techniques, continues to increase our understanding of bacterioplankton temporal dynamics.
Microbial Observatory at North Temperate Lakes LTER Spatial and temporal cyanobacterial population dynamics in Lake Mendota 2009 - 2011
Toxic cyanobacterial blooms threaten freshwaters worldwide but have proven difficult to predict because the mechanisms of bloom formation and toxin production are unknown, especially on weekly time scales. Water quality management continues to focus on aggregated metrics, such as chlorophyll and total nutrients, which may not be sufficient to explain complex community changes and functions such as toxin production. For example, nitrogen (N) speciation and cycling play an important role, on daily time scales, in shaping cyanobacterial communities because declining N has been shown to select for N fixers. In addition, subsequent N pulses from N2 fixation may stimulate and sustain toxic cyanobacterial growth. Herein, we describe how rapid early summer declines in N followed by bursts of N fixation have shaped cyanobacterial communities in a eutrophic lake (Lake Mendota, Wisconsin, USA), possibly driving toxic Microcystis blooms throughout the growing season. On weekly time scales in 2010 and *2011, we monitored the cyanobacterial community in a eutrophic lake using the phycocyanin intergenic spacer (PC-IGS) region to determine population dynamics. In parallel, we measured microcystin concentrations, N2 fixation rates, and potential environmental drivers that contribute to structuring the community.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.