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216 results for “endoplasmic reticulum”
Fam134c and Fam134b shape axonal endoplasmic reticulum architecture in vivo
<p>Endoplasmic reticulum (ER) remodeling is vital for cellular organization. ER-phagy, a selective autophagy targeting ER, plays an important role in maintaining ER morphology and function. The FAM134 protein family, including FAM134A, FAM134B, and FAM134C, mediates ER-phagy. While FAM134B mutations are linked to hereditary sensory and autonomic neuropathy in humans, the physiological role of the other FAM134 proteins remains unknown. To address this, we investigated the roles of FAM134 proteins using single and combined knockouts (KOs) in mice. Single KO in young mice showed no major phenotypes, but combined <em>Fam134b</em> and <em>Fam134c</em> deletion (<em>Fam134b/c<sup>dKO</sup>)</em> led to rapid neuromuscular and somatosensory degeneration, resulting in premature death. <em>Fam134b/c<sup>dKO</sup></em> mice show rapid loss of motor and sensory axons in the peripheral nervous system. Long axons from <em>Fam134b/c<sup>dKO</sup></em> mice exhibited expanded tubular ER with a transverse ladder-like appearance, whereas no obvious abnormalities were observed in cortical ER. Our study unveils critical roles of FAM134C and FAM134B in the formation of tubular ER network in axons of both motor and sensory neurons.</p>
Multi-omic Mapping of Human Pancreatic Islet Endoplasmic Reticulum and Cytokine Stress Responses Provide Type 2 Diabetes Genetic Insights
<p>The following data accompanying the manuscript can be found here:</p> <ul> <li>Expression (bulk RNA-seq & scRNA-seq) matrices</li> <li>Chromatin accessibility (bulk ATAC-seq) matrices</li> <li>TDF files for IGV browser visualization of cis-regulatory elements</li> </ul>
Immunization of Patients With Metastatic Melanoma Using the GP100 Peptide Preceded by an Endoplasmic Reticulum Insertion Signal Sequence
ClinicalTrials.gov study NCT00001705. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Study of the Expression of Endoplasmic Reticulum Stress During Extracorporeal Circulation in Humans
ClinicalTrials.gov study NCT03722979. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Relationship of Endoplasmic Reticulum Stress and Tonsillar Tissue Diseases
ClinicalTrials.gov study NCT04653376. IPD Sharing: UNDECIDED. Countries: 1. Publications: 3.
Involvement of Reticulum Endoplasmic Stress in the Physiopathology of Polycystic Ovary Syndrome
ClinicalTrials.gov study NCT02302326. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Relationship of Endoplasmic Reticulum Stress and Adenoid Diseases
ClinicalTrials.gov study NCT04583631. IPD Sharing: UNDECIDED. Countries: 1. Publications: 2.
Data from: A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress
Open the record for dataset details and reuse information.
Data from Ca2+ mobilization-dependent reduction of the endoplasmic reticulum lumen is due to influx of cytosolic glutathione
<p>Research data associated with the manuscript "Ca2+ mobilization-dependent reduction of the endoplasmic reticulum lumen is due to influx of cytosolic glutathione" that is under consideration at BMC Biology.</p> <p>Data files are associated with eight main figures and four supplementary figures in the paper. For data reuse and file naming convention, note the read me.txt file.</p>
Selenoprotein N is an endoplasmic reticulum calcium sensor that links luminal calcium levels to a redox activity
<p>Data files for Manuscript "Selenoprotein N is an endoplasmic reticulum calcium sensor that links luminal calcium levels to a redox activity" by Chernorudskiy et al., 2020</p>
BRD4-mediated epigenetic regulation of endoplasmic reticulum-mitochondria contact sites is governed by the mitochondrial complex III
<p>This is a high content drug screening dataset composed of images and single-cell analysis results from a 384-well plate </p> <p>The SPLICS reporter is composed of a split GFP1-10 barrel localized to the mitochondrial outer membrane, a P2A self-cleavage signal for equimolar expression, and the remaining beta11 fragment targeted to the ER membrane. Upon ERMCS formation within the 8-10 nm distance, the split-GFP reporter forms intact GFP. Doxycycline-inducible SPLICS reporter stable cell lines were generated via a three-plasmid PiggyBac transposase system. Cells were co-transfected with the plasmids using Lipofectamine 2000 transfection reagent, then the next day selected with 2 mg/ml of G418 (Geneticin) for 7 days. With the SPLICS integrated cells, mitochondrial TagRFP is expressed in them. For mitochondrial TagRFP labeling in cells, we utilized a lentiviral backbone and introduced a mitochondria matrix TagRFP (mitoTagRFP) to control for gene expression and monitor mitochondrial abundance. After mitoTagRFP integration, 100 ng/ml of doxycycline were used to induce SPLICS reporter expression, and GFP/TagRFP double positive cells were sorted on Bigfoot Spectral Cell Sorter (Invitrogen) to isolate GFP/TagRFP positive clones. Clones were cultured and screened for accurate localization of SPLICS reporter to mitochondria and ER via live cell imaging and for normal mitochondrial oxygen consumption rate. Probe induction did not have a deleterious effect on cell number or increasing green fluorescence for at least 72 hours of reporter induction. For ERMCS analysis with SPLICS, cells will be induced with 100 ng/ml of doxycycline for minimally 24 hr before treatment or analysis.</p>
Figure 5 from: Abbas AH, Mahmood AAR, Tahtamouni LH, Al-Mazaydeh ZA, Rammaha MS, Alsoubani F, Al-bayati RI (2021) A novel derivative of picolinic acid induces endoplasmic reticulum stress-mediated apoptosis in human non-small cell lung cancer cells: synthesis, docking study, and anticancer activity. Pharmacia 68(3): 679-692. https://doi.org/10.3897/pharmacia.68.e70654
Figure 5 Compound 5 induces the ER-mediated apoptosis. A) Representative Western blot showing the enhanced release of Smac/DIABLO but not cytochrome c from the mitochondria (MF) into the cytosol (CF) of compound 5-treated A549 lung cancer cells (GI50). Equal protein loading was controlled by staining membranes with Ponceau S (a representative section of the stained membrane is shown). The experiment was repeated three times and the corresponding quantification is shown in (B), B) Quantification of smac/DIABLO and cytochrome C levels in vehicle-treated control A549 cells and cells treated with GI50 amount of compound 5 or 5% DMSO as a negative control. M: mitochondria fraction; C: cytosol fraction. Scale bars: mean ± SEM of three independent experiments. ** p < 0.01 compared to vehicle-treated control cells, C) Representative Western blot showing the induction of phosphorylation of eukaryotic initiation factor-2 (eIF-2) in A549 cells treated with GI50 amounts of compound 5. For comparison purposes, thapsigargin (TG), an ER stress-causing drug was used (3 μM, 2 h) as a positive control. The experiment was repeated three times.
Figure 4 from: Abbas AH, Mahmood AAR, Tahtamouni LH, Al-Mazaydeh ZA, Rammaha MS, Alsoubani F, Al-bayati RI (2021) A novel derivative of picolinic acid induces endoplasmic reticulum stress-mediated apoptosis in human non-small cell lung cancer cells: synthesis, docking study, and anticancer activity. Pharmacia 68(3): 679-692. https://doi.org/10.3897/pharmacia.68.e70654
Figure 4 Compound 5 causes caspase activation. A) qRT-PCR analysis of caspase 3, 4, 8 and 9 mRNA in compound 5-treated cells (GI50) as compared to vehicle-treated control cells [set as 1 arbitrary unit (a.u.)]. Values were normalized to β-actin. Scale bars: mean ± SEM of three independent experiments performed in triplicates. *p < 0.05, **p < 0.01 compared to vehicle-treated control cells, B) Representative Western blots showing cleavage "activation' of procaspase 3 to the active form p17, procaspase 4 to the active form p20, and procaspase 9 to the active forms p35/p37. C: vehicle-treated control cells; 0.5%: 0.5% DMSO-treated cells; 1%: 1% DMSO-treated cells; 5%: 5% DMSO-treated cells; C.5: Compound 5-treated cells. The experiment was repeated three times.
Figure 2 from: Abbas AH, Mahmood AAR, Tahtamouni LH, Al-Mazaydeh ZA, Rammaha MS, Alsoubani F, Al-bayati RI (2021) A novel derivative of picolinic acid induces endoplasmic reticulum stress-mediated apoptosis in human non-small cell lung cancer cells: synthesis, docking study, and anticancer activity. Pharmacia 68(3): 679-692. https://doi.org/10.3897/pharmacia.68.e70654
Figure 2 The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) assay results for compound 5 against MCF10A cells, and white blood cells (WBC) after 24, 48 and 72 h of treatment.
Figure 3 from: Abbas AH, Mahmood AAR, Tahtamouni LH, Al-Mazaydeh ZA, Rammaha MS, Alsoubani F, Al-bayati RI (2021) A novel derivative of picolinic acid induces endoplasmic reticulum stress-mediated apoptosis in human non-small cell lung cancer cells: synthesis, docking study, and anticancer activity. Pharmacia 68(3): 679-692. https://doi.org/10.3897/pharmacia.68.e70654
Figure 3 Compound 5 induces apoptosis in A549 lung cancer cells. A) Fluorescence images of A549 lung cancer cells showing fragmented nuclei after DAPI staining. Scale bar: 10 µm, B) Detection of DNA fragmentation by agarose gel electrophoresis. Cells were treated and genomic DNA was extracted and electrophoresed on 1.5% agarose gels. M: DNA molecular weight marker; C: vehicle-treated control cells; 1%: 1% DMSO-treated cells; 5%: 5% DMSO-treated cells; C.5: Compound 5-treated cells. Arrows indicate DNA fragments. Three experiments were performed with similar results.
Figure 6 from: Abbas AH, Mahmood AAR, Tahtamouni LH, Al-Mazaydeh ZA, Rammaha MS, Alsoubani F, Al-bayati RI (2021) A novel derivative of picolinic acid induces endoplasmic reticulum stress-mediated apoptosis in human non-small cell lung cancer cells: synthesis, docking study, and anticancer activity. Pharmacia 68(3): 679-692. https://doi.org/10.3897/pharmacia.68.e70654
Figure 6 3D View of the target compounds (5, 7A, 6B and 6C) docked against ATP binding site of EGFR kinase.
Single cell transcriptomics of vomeronasal neuroepithelium reveals a differential endoplasmic reticulum environment amongst neuronal subtypes
GEO Series GSE253252. Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing.
Loss of dihydroceramide desaturase drives neurodegeneration by disrupting endoplasmic reticulum and lipid droplet homeostasis in glial cells
GEO Series GSE263308. Drosophila melanogaster. 10 samples. Type: Expression profiling by high throughput sequencing.
Endoplasmic reticulum stress regulates immunosuppressive function of myeloid derived suppressor cells in leprosy that can be overcome by IFNg production
GEO Series GSE129033. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
Overexpression of PTHrP in beta cells reveals key effects on endoplasmic reticulum stress
GEO Series GSE295339. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.