Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
324
datasets available to search
ShareScore release 0.9.0
Dataset results
324 results for “fluorescent imaging”
(05)-Strobl2018A-DS0001 – Tribolium castaneum AGOC{Zen1'#O(LA)-mEmerald} #2 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(05)-Strobl2018A-DS0001 – <em>Tribolium castaneum</em> AGOC{Zen1'#O(LA)-mEmerald} #2 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(05)-Strobl2018A-DS0003 – Tribolium castaneum AGOC{ARP5'#O(LA)-mEmerald} #2 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(05)-Strobl2018A-DS0003 – <em>Tribolium castaneum</em> AGOC{ARP5'#O(LA)-mEmerald} #2 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(05)-Strobl2018A-DS0002 – Tribolium castaneum AGOC{ARP5'#O(LA)-mEmerald} #1 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(05)-Strobl2018A-DS0002 – <em>Tribolium castaneum</em> AGOC{ARP5'#O(LA)-mEmerald} #1 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
Time-lapse (4D) volumetric fluorescence microscopy image sequence of a living zebrafish embryo
<p>The dataset contains a time-lapse (4D) volumetric fluorescence microscopy image sequence of a living zebrafish embryo (cxcr4aMO). The sequence has been captured with a confocal laser-scanning microscope during zebrafish gastrulation and shows endodermal cells that have been fluorescently labelled.</p> <p>The sequence is best viewed with Fiji (https://fiji.sc/) and can be loaded into Matlab with tiffread.m (http://www.cytosim.org/misc/index.html).</p> <p>For the treatment of the specimen see:</p> <p>S. Nair and T. F. Schilling. Chemokine signaling controls endodermal migration during zebrafish gastrulation. Science, 322(5898):89–92, October 2008.</p>
Sparks et al, Heterogeneity in tumor chromatin-doxorubicin binding revealed by in vivo fluorescence lifetime imaging confocal endomicroscopy: In vitro data
<p>Data is divided into three folders:</p> <ul> <li>Sparks_et_al_FIG2_Histone_vs_free_GFP <ul> <li>data for Sparks et al Figure 2</li> <li>main text section: <em>'FRET between chromatin-bound GFP and doxorubicin'</em></li> </ul> </li> <li>Sparks_et_al_FIG3_in_vitro_dose_response <ul> <li>data for Sparks et al Figure 3#</li> <li>main text section:<em> 'FLIM endomicroscope can monitor doxorubicin cellular uptake'</em></li> </ul> </li> <li>Sparks_et_al_SuppFIG2_endoscope_spectral_cross_talk <ul> <li>data for Sparks et al Supplementary Figure 2</li> <li>Supplementary information</li> </ul> </li> </ul> <p><strong>Cell lines</strong></p> <p>IGROV-1 cell lines were cultured in CO<sub>2</sub> dependent media with 10% fetal bovine serum and 1% Pen Strep at 37 ˚C. Before experiments, cells were grown to 80% confluence. For measuring doxorubicin uptake by fluorescence an IGROV-1 cell line stably expressing GFP fused to Histone-1 (H1) was made using the PiggyBac transposon system. As a control to show that effect of doxorubicin on GFP depends on whether it is fused to H1 or not, a stable whole cell expression of GFP by lentiviral transfection and selection by Geneticin was made. For bioluminescence imaging of xenograft tumors, all IGROV-1 cell lines were made to stably express firefly luciferase.</p> <p>To investigate the effect of doxorubicin on other histones, IGROV-1 cells were transiently transfected with a Histone-2B-GFP plasmid (gift from Kurt Anderson) using the Lipofectamine® 2000 reagent.</p> <p>IGROV-1 cells were obtained from Crick institute cell services and confirmed as IGROV-1 by Short Tandem Repeats (STR) profiling and no mycoplasma was detected.</p> <p><strong>In vitro experiments</strong></p> <p>IGROV-1 cells were grown to 80% confluence in 75 ml flasks before being re-plated in 12 or 24 well plates or 35 ml glass bottomed dishes and allowed to attach to the surface for 24 hours before experiments.</p> <p>To study how the fluorescence of GFP labelled H1 labelled IGROV-1 cells changes with doxorubicin treatment, fluorescence intensity and lifetime distributions were measured from cells after 3 hours of incubation with doxorubicin of varying concentrations (0, 0.18, 0.9, 1.8, 9, 18 µM) by serial dilutions of a stock solution with PBS. After 3 of hours, cells were washed in PBS then fixed for 20 minutes in 4% PFA. Cells were then imaged in PBS. Doxorubicin hydrochloride (Sigma-Aldrich, D1515-10 mg) was dissolved in PBS to a concentration of 9 mM and stored at -20˚C.</p>
Sparks et al, Heterogeneity in tumor chromatin-doxorubicin binding revealed by in vivo fluorescence lifetime imaging confocal endomicroscopy: in vivo data
<p>Data is divided into three folders:</p> <ul> <li>Sparks_et_al_FIG_6_IP_intranodule_heterogeneity <ul> <li>data for Sparks et al Figure 6</li> <li>main text section: <em>'FRET between chromatin-bound GFP and doxorubicin'</em></li> </ul> </li> <li>Sparks_et_al_FIG4_5_6_IP_IV_chemo_comparison <ul> <li>data for Sparks et al Figures 4,5 & 6</li> <li>main text section:<em> 'FLIM endomicroscope can monitor doxorubicin cellular uptake'</em></li> </ul> </li> <li>Sparks_et_al_FIG6_IP__internodule_heterogeneity <ul> <li>data for Sparks et al Figure 6</li> <li>main text section: <em>'Intra-tumor heterogeneity'</em></li> </ul> </li> </ul> <p><strong>In vivo experiments</strong></p> <p>Murine xenografts were prepared by intraperitoneal (IP) injection of IGROV-1 cancer cells. IGROV-1 cells were grown to 80% confluence before being trypsinized and re‑suspended in PBS at a concentration of cells per ml. cells were injected into ICRF nude mice. After 14 days post-injection, the presence of intraperitoneal tumors was confirmed by bioluminescence imaging. Briefly, an IVIS bioluminescence imaging system was used to image isoflurane anesthetized mice. 100 µl of D-luciferin (luciferase substrate) at 30mg ml<sup>-1</sup> was injected IP 10 minutes before recording of bioluminescence images. The presence of peritoneal tumors was confirmed if bioluminescence signals from the peritoneum were above background noise 10-30 minutes after D‑luciferin injections. Following confirmation of tumors, in vivo fluorescence imaging experiments were carried out after 21 days. To study differences in drug uptake between intravenous or intraperitoneal delivery, prior to imaging mice were subject to IP or IV doxorubicin-based chemotherapy for 1.5, 3 or 24 hours. Imaging involved terminal procedures, mice were anesthetized then peritoneal tumors were exposed by minor surgery and inspected with the CEM.</p> <p>All animal model procedures were approved by The Francis Crick Institute Biological Ethics Committee and UK Home Office authority provided by Project License 70/8380.</p> <p> </p> <p> </p>
(06)-He2019A-DS0003 – Tribolium castaneum AGOC #6 subline × foxQ2-5' line long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(06)-He2019A-DS0003 – <em>Tribolium castaneum</em> AGOC #6 subline × foxQ2-5' line long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(06)-He2019A-DS0002 – Tribolium castaneum foxQ2-5' line × AGOC #6 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(06)-He2019A-DS0002 – <em>Tribolium castaneum</em> foxQ2-5' line × AGOC #6 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(09)-Pereyra2021A-DS0001--DS0003 – Three Tribolium castaneum long-term live imaging datasets of embryonic development acquired with light sheet fluorescence microscopy
<p>(09)-Pereyra2021A-DS0001--DS0003 – Three <em>Tribolium castaneum</em> long-term live imaging datasets of embryonic development acquired with light sheet fluorescence microscopy</p>
(08)-Strobl2021A-DS0002 – Tribolium castaneum ACOS{ATub'H2B-mRuby} #1 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(08)-Strobl2021A-DS0002 – <em>Tribolium castaneum</em> ACOS{ATub'H2B-mRuby} #1 subline long-term live imaging data of embryonic development acquired with light sheet fluorescence microscopy</p>
(08)-Strobl2021A-DS0001 – Tribolium castaneum AGOC{Zen1'#O(LA)-mEmerald} #1 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(08)-Strobl2021A-DS0001 – <em>Tribolium castaneum</em> AGOC{Zen1'#O(LA)-mEmerald} #1 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(07)-Ratke2020A-DS0005 – Tribolium castaneum AGOC{Zen1'#O(LA)-mEmerald} #2 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(07)-Ratke2020A-DS0005 – <em>Tribolium castaneum</em> AGOC{Zen1'#O(LA)-mEmerald} #2 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(07)-Ratke2020A-DS0004 – Tribolium castaneum AGOC{Zen1'#O(LA)-mEmerald} #1 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(07)-Ratke2020A-DS0004 – <em>Tribolium castaneum</em> AGOC{Zen1'#O(LA)-mEmerald} #1 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(07)-Ratke2020A-DS0003 – Drosophila melanogaster w[*]; P{w[+mC]=His2Av-EGFP.C}2/SM6a line long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(07)-Ratke2020A-DS0003 – <em>Drosophila melanogaster</em> w[*]; P{w[+mC]=His2Av-EGFP.C}2/SM6a line long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(07)-Ratke2020A-DS0001 – Drosophila melanogaster y[1] w[67c23]; P{w[+mC]=Ubi-GFP.nls}ID-2; P{Ubi-GFP.nls}ID-3 line long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(07)-Ratke2020A-DS0001 – <em>Drosophila melanogaster</em> y[1] w[67c23]; P{w[+mC]=Ubi-GFP.nls}ID-2; P{Ubi-GFP.nls}ID-3 line long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(07)-Ratke2020A-DS0002 – Drosophila melanogaster w[*]; P{w[+mC]=Tub84B-EGFP.NLS}3 long-term live imaging dataset acquired with light sheet fluorescence microscopy
<p>(07)-Ratke2020A-DS0002 <em>–</em> <em>Drosophila melanogaste</em>r y[1] w[67c23]; P{w[+mC]=Ubi-GFP.nls}ID-2; P{Ubi-GFP.nls}ID-3 (Bloomington <em>Drosophila</em> Stock Center #29724) long-term live imaging dataset acquired with light sheet fluorescence microscopy</p>
(08)-Strobl2021A-DS0003 – Tribolium castaneum Gruul #1 hybrid line long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(08)-Strobl2021A-DS0003 – <em>Tribolium castaneum</em> Gruul #1 hybrid line long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(07)-Ratke2020A-DS0006 – Tribolium castaneum AGOC{Zen1'#O(LA)-mEmerald} #3 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(07)-Ratke2020A-DS0006 – <em>Tribolium castaneum</em> AGOC{Zen1'#O(LA)-mEmerald} #3 subline long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
Linked collectors and determiners for: Taxonomic synthesis of the eastern North American millipede genus Pseudopolydesmus (Diplopoda: Polydesmida: Polydesmidae), utilizing high-detail ultraviolet fluorescence imaging.
Natural history specimen data linked to collectors and determiners held within, "Taxonomic synthesis of the eastern North American millipede genus Pseudopolydesmus (Diplopoda: Polydesmida: Polydesmidae), utilizing high-detail ultraviolet fluorescence imaging". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="https://bionomia.net/dataset/7226548c-af21-4648-85ea-733acdfda22e">https://bionomia.net/dataset/7226548c-af21-4648-85ea-733acdfda22e</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/7226548c-af21-4648-85ea-733acdfda22e">https://gbif.org/dataset/7226548c-af21-4648-85ea-733acdfda22e</a>. Formatted as a Frictionless Data package.
Dataset for Adaptive Light-Sheet Fluorescence Microscopy with a Deformable Mirror for Video-Rate Volumetric Imaging
<p>1. Underlying data of figures in the paper </p> <p>2. Background images used to process the experimental data</p> <p>3. image stack of 250 nm beads</p> <p>4. image stack of sunflower pollen grains</p> <p>5. image stacks and videos of Fluo-4 labelled cells</p> <p>6. image stacks and videos of CMO-labelled cells</p> <p>The data is organised according to the figures they are related to in the following publication:</p> <p> </p> <p><a href="https://aip.scitation.org/author/Hong%2C+Wenzhi">Wenzhi Hong</a><em>, </em><a href="https://aip.scitation.org/author/Wright%2C+Terry">Terry Wright</a><em>, </em><a href="https://aip.scitation.org/author/Sparks%2C+Hugh">Hugh Sparks</a><em>, </em><a href="https://aip.scitation.org/author/Dvinskikh%2C+Liuba">Liuba Dvinskikh</a><em>, </em><a href="https://aip.scitation.org/author/MacLeod%2C+Ken">Ken MacLeod</a><em>, </em><a href="https://aip.scitation.org/author/Paterson%2C+Carl">Carl Paterson</a><em>, and </em><a href="https://aip.scitation.org/author/Dunsby%2C+Chris">Chris Dunsby</a> </p> <p>, "Adaptive light-sheet fluorescence microscopy with a deformable mirror for video-rate volumetric imaging", Appl. Phys. Lett. 121, 193703 (2022) <a href="https://doi.org/10.1063/5.0125946">https://doi.org/10.1063/5.0125946</a></p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.