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324 results for “fluorescent imaging”
Multiplexed fluorescence imaging based on cycles, raw and processed data.
<p>This dataset was created from a larger acquisition in order to provide an example of reasonnable size, as a companion data set to the F1000Research paper preprint DOIXXX.</p> <ul> <li>The original raw data including metadata files are included in <strong>Microscope_Output.zip.</strong></li> <li><strong>Experiment.json</strong> and<strong> channelnames.txt </strong>are the ones generated by the acquisition software. They are the only files needed when starting from one of the processed data set below.</li> <li>The deconvolution obtained with the commercial software Microvolution is also provided in <strong>bu_deconvolution.zip.</strong> To start from Step 1(Extended Depth of Field) instead of Step 0 (deconvolution), unzip this file in your output directory and rename the folder bu_deconvolution to out.</li> <li>The extended field of view 2D images created from step 0 to step 2, provided for convenince in <strong>edfonly.zip</strong></li> <li>The final files generated by trhe Multiplex processor, including the segmentation mask , are provided in<strong> finaloutput.zip</strong>. These files can be used in a specific analysis software.</li> </ul> <p> </p>
Confocal fluorescence microscopy images of the lacuno-canalicular network in bone femoral diaphysis of mice from the BionM1 project (space flight)
<p>This data set provides complementary measurements to a separate THG data set of the same study: doi: 10.5281/zenodo.1475906</p> <p>Data set for 1 sample of each of the 3 groups: Control, Space Flight and Synchro (ground control with space flight housing and feeding conditions). Contains confocal fluorescence microscopy images in tif format of 2D mosaic of selected samples and 3D stacks in selected anatomical regions of interest. See readme file for more information.</p>
Super-Resolved FRET Imaging by Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy
<p>FRET-based methods are a special tool for detecting interactions between (bio)molecules and their immediate environment. The spatial distribution of molecular interactions and functional states can be seen using FLIM (Fluorescence Lifetime IMaging) and FRET imaging. The spatial information, accuracy, and dynamic range of the observed signals are, however, constrained by the fact that conventional FLIM and FRET imaging only provides average information over an ensemble of molecules within a diffraction-limited volume. On the other hand, conventional Single Molecule Localization Microscopy (SMLM) relies on highly sensitive multi-pixel detectors (e.g. sCMOS or EM-CCD) whose time resolution is not suitable for fluorescence lifetime measurements.</p> <p>Here, we demonstrate a method for obtaining super-resolved FRET imaging using confocal fluorescence-lifetime single-molecule localization microscopy. The proof of concept was carried out using a DNA origami sample for performing DNA-PAINT measurements in combination with fluorogenic probes for reducing background signal. With this method, We show that FRET events separated by sub-diffraction distances can be distinguished based on lifetime modifications.</p>
Quantification of ROIs corresponding to MQs from fluorescence in vivo imaging experiments
<p>We injected DIr labelled macrophages into mice carring immunolgical hot and cold KPC pancreatic tumors and quantified the recruitment to the tumor sites and lungs of the injected cells at different days after injection using fluorescence imaging. We hypotesized that macrophages would be recruited into tumor tissue and in prevalence into cold tumors.</p>
Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes
<p>Raw multichannel and/or Z-stack source data from time series images in TIF format to accompany publication of:</p> <p><strong>Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes</strong></p> <p>Jina Ko<sup>1</sup>, Martin Wilkovitsch<sup>2</sup>, Juhyun Oh<sup>1</sup>, Rainer Kohler<sup>1</sup>, Evangelia Bolli<sup>1,3</sup>, Mikael J. Pittet<sup>1,3,4,5</sup>, Claudio Vinegoni<sup>1</sup>, David B. Sykes<sup>6,7</sup>, Hannes Mikula<sup>2</sup>, Ralph Weissleder<sup>1,8</sup>*, Jonathan C. T. Carlson<sup>1,7</sup>*</p> <p><sup>1 </sup>Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114 </p> <p><sup>2</sup> Institute of Applied Synthetic Chemistry, TU Wien, 1060 Vienna, Austria </p> <p><sup>3</sup> Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland</p> <p><sup>4</sup> Ludwig Institute for Cancer Research, Lausanne Branch, Switzerland</p> <p><sup>5</sup> AGORA Cancer Center, Lausanne, Switzerland</p> <p><sup>6</sup> Center for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA</p> <p><sup>7 </sup>Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA</p> <p><sup>8 </sup>Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115</p>
Insights into metabolic changes during epidermal differentiation as revealed by multiphoton microscopy with fluorescence lifetime imaging
<p>Rapid developments in the field of organotypic cultures has generated a growing need for effective quality control measures during tissue development. In this study, we correlate metabolic changes with epidermal differentiation and demonstrate that multiphoton microscopy with fluorescence lifetime imaging (MPM-FLIM) can be applied as a non-invasive approach to monitor epidermal differentiation of keratinocytes with respect to proliferative and differentiated states. Keratinocytes grown at 1.5 mM Ca2+ exhibited increased expression of differentiation markers KRT1 and KRT10 compared to 60 μM Ca2+, and a metabolic shift from glycolysis to mitochondrial respiration. Fitting the fluorescence decay with a biexponential model revealed a decreased relative fraction of intracellular NADH and FAD after high calcium treatment, consistent with increased oxidative phosphorylation. Using these two parameters, the epidermal differentiation process could be monitored over a 96 h period. Implementing discriminating analysis based on k-means clustering generated clusters that correlated well with culturing time, suggesting that this methodology can be employed as part of an automated pipeline for monitoring keratinocyte differentiation.</p>
STORM imaging of Bacillus subtilis labeled by fluorescent d-amino acids
<p>Bacillus subtilus cells were labeled by fluorescent d-amino acids, followed by STORM super-resolution imaging.</p> <p>The wide-field image and STORM imaging stack are uploaded.</p>
X-ray Fluorescence Ghost Imaging - CuSn mask - Three Wires (Fe & Cu)
<p>X-ray Fluorescence Ghost Imaging (XRF-GI) dataset of three wires (one Fe, and two Cu) in a plastic capillary. The capillary contains trace elements like Zn, Zr, etc.</p> <p>The GI scan is presented in the following article <a title="Synchrotron-based x ray fluorescence ghost imaging" href="https://doi.org/10.1364/OL.499046">10.1364/OL.499046</a>. A total of 896 GI realizations were taken, organized into 16 vertical translations and 56 horizontal translations of the structuring element (CuSn mask).<br>The dataset contains both the sample transmission images and the masks plus sample transmission images. No images of the masks are provided (they need to be computed).</p> <p>The data is organized in an HDF5 file, under the following structure:</p> <pre><code>dataset_CuSn-mask_3wires.h5 │ ├data │ ├flat_panel │ │ ├dark [float32: 16 × 170 × 350] │ │ ├empty_beam [float32: 170 × 350] │ │ ├sample [float32: 16 × 170 × 350] │ │ └sample_and_masks [float32: 16 × 56 × 170 × 350] │ └xrf [float32: 16 × 56 × 4096] │ └metadata └xrf ├bias_keV [float64: scalar] ├gain_keV [float64: scalar] └ranges ├Ca [int64: 2] ├Cu [int64: 2] ├Fe [int64: 2] ├Si [int64: 2] ├Ti [int64: 2] ├Zn [int64: 2] └Zr [int64: 2] </code></pre> <p>The meaning of the paths is:</p> <ul> <li><code>/data/xrf</code> contains the XRF spectra for each GI realization</li> <li><code>/data/flat_panel/dark</code> contains the dark images of each scan line (no beam)</li> <li><code>/data/flat_panel/empty_beam</code> contains the empty beam (no sample & no masks) intensity distribution</li> <li><code>/data/flat_panel/sample</code> contains the transmission images of the sample at each scan line</li> <li><code>/data/flat_panel/sample</code>_and_masks contains the transmission images of the sample and masks at each GI realization</li> <li><code>/metadata/xrf/bias_keV</code> contains the bias in keV of the XRF spectrum</li> <li><code>/metadata/xrf/gain_keV</code> contains the gain in keV of each XRF energy bin</li> <li><code>/metadata/xrf/ranges/</code> contains the bin ranges for interesting K<sub>alpha</sub> elemental emission lines in the XRF spectrum</li> </ul> <p>For further information we refer to the associated publication.</p> <p>The data can be processed with structured illumination routines of the code at: <a href="https://github.com/cicwi/PyCorrectedEmissionCT">https://github.com/cicwi/PyCorrectedEmissionCT</a>.</p>
Fluorescent Microglia Images for Analyzing Morphological Changes due to Injury Duration in the Ischemic Rat Brain
<p>The image data included in this dataset are the confocal microscope images (converted from original .nd2 file to .tiff form) used for the publication: Joseph, A., Liao, R., Zhang, M., Helmbrecht, H., McKenna, M., Filteau, J. R., & Nance, E. (2020). Nanoparticle-microglial interaction in the ischemic brain is modulated by injury duration and treatment. <em>Bioengineering & translational medicine</em>, <em>5</em>(3), e10175. https://doi.org/10.1002/btm2.10175</p> <p>The data is organized by brain slice number, region, and image number. There is also an included excel file 'datadescriptions.xlsx' that provides more information about the metadata of the dataset. </p> <p> </p> <p>The data was procured and processed by the Disease Directed Engineering Lab, PI: Elizabeth Nance, at the University of Washington.</p>
Source files and reconstructions for "Simple 3D compressed sensing scheme for faster and less phototoxic fluorescence microscopy imaging"
<p>Source files and reconstructions for "Simple 3D compressed sensing scheme for faster and less phototoxic fluorescence microscopy imaging"</p> <p>The source files are to be used with the code on https://github.com/MaximeMaW/CompressedSensingMicroscopy3D (also archived in https://zenodo.org/record/439690)</p> <ol> <li>The files prefixed with "VIZ" are high resolution TIF visualizations.</li> <li>The files come from three experiments on two different setups: <ol> <li>A lattice light sheet microscope (LLSM): beads sample (filed termed "<strong>lattice-beads</strong>" and actin-labelled mESCs (files termed "<strong>lattice-phalloidin</strong>")</li> <li>An epifluorescence microscope: beads sample (files termed "<strong>epifluorescence</strong>")</li> </ol> </li> <li>The acquisitions were either performed using an identity measurement matrix (mimicking the plane-by-plane acquisition mode of a traditional z-stack): files termes "<strong>reference</strong>" or with a Fourier measurement matrix (described in the code mentioned above) with a compression ratio of 2 (files termed "<strong>compressed</strong>".</li> <li>The reconstructions were performed as described in the paper with the code mentioned above. Several reconstructions were computed from the same compressed images by simulating increasing compression ratios. To do so, reconstructions were performed by selecting a subset of the acquired planes (number indicated as "<strong>**frames</strong>")</li> <li>Reconstructions were sparsified using a 2D PSF model computed for our epifliuorescence setup and the LLSM (files termed "<strong>PSF_model</strong>"). These are provided as numpy arrays.</li> </ol> <p> </p>
(10)-Strobl2022A-DS0008 – Ceratitis capitata TREhs43-hid^Ala5_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(10)-Strobl2022A-DS0008 – <em>Ceratitis capitata</em> TREhs43-hid<sup>Ala5</sup>_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(10)-Strobl2022A-DS0004 – Ceratitis capitata TREhs43-hid^Ala5_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(10)-Strobl2022A-DS0004 – <em>Ceratitis capitata</em> TREhs43-hid<sup>Ala5</sup>_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(10)-Strobl2022A-DS0001 – Ceratitis capitata TREhs43-hid^Ala5_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(10)-Strobl2022A-DS0001 – <em>Ceratitis capitata</em> TREhs43-hid<sup>Ala5</sup>_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(10)-Strobl2022A-DS0003 – Ceratitis capitata TREhs43-hid^Ala5_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(10)-Strobl2022A-DS0003 – <em>Ceratitis capitata</em> TREhs43-hid<sup>Ala5</sup>_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(10)-Strobl2022A-DS0002 – Ceratitis capitata TREhs43-hid^Ala5_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(10)-Strobl2022A-DS0002 – <em>Ceratitis capitata</em> TREhs43-hid<sup>Ala5</sup>_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(10)-Strobl2022A-DS0007 – Ceratitis capitata TREhs43-hid^Ala5_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(10)-Strobl2022A-DS0007 – <em>Ceratitis capitata</em> TREhs43-hid<sup>Ala5</sup>_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(10)-Strobl2022A-DS0009 – Ceratitis capitata TREhs43-hid^Ala5_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(10)-Strobl2022A-DS0009 – <em>Ceratitis capitata</em> TREhs43-hid<sup>Ala5</sup>_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(10)-Strobl2022A-DS0006 – Ceratitis capitata TREhs43-hid^Ala5_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(10)-Strobl2022A-DS0006 – <em>Ceratitis capitata</em> TREhs43-hid<sup>Ala5</sup>_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
(10)-Strobl2022A-DS0005 – Ceratitis capitata TREhs43-hid^Ala5_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy
<p>(10)-Strobl2022A-DS0004 – <em>Ceratitis capitata</em> TREhs43-hid<sup>Ala5</sup>_F1m2 long-term live imaging dataset of embryonic development acquired with light sheet fluorescence microscopy</p>
Code and data for: Decoupling channel count from field-of-view and spatial resolution in single-sensor imaging systems for fluorescence image-guided surgery
<p><em>Significance</em></p> <p>Near-infrared fluorescence image-guided surgery is often thought of as a spectral imaging problem where the channel count is the critical parameter, but it should also be thought of as a multiscale imaging problem where the field-of-view and spatial resolution are similarly important.</p> <p><em>Aim</em></p> <p>Conventional imaging systems based on division-of-focal-plane architectures suffer from a strict relationship between the channel count on one hand and the field-of-view and spatial resolution on the other, but bioinspired imaging systems that combine stacked photodiode image sensors and long-pass/short-pass filter arrays offer a weaker tradeoff.</p> <p><em>Approach</em></p> <p>In this paper, we explore how the relevant changes to the image sensor and associated image processing routines affect image fidelity during image-guided surgeries for tumor removal in an animal model of breast cancer and nodal mapping in women with breast cancer.</p> <p><em>Results</em></p> <p>We demonstrate that a transition from a conventional imaging system to a bioinspired one, along with optimization of the image processing routines, yields improvements in multiple measures of spectral and textural rendition relevant to surgical decision-making.</p> <p><em>Conclusions</em></p> <p>These results call for a critical examination of the devices and algorithms that underpin image-guided surgery to ensure that surgeons receive high-quality guidance and patients receive high-quality outcomes as these technologies enter clinical practice.</p>
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