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305 results for “gene clusters”
Microscopy data: interaction of the gene iscub with RNA polymerase II clusters during early zebrafish embryo development
<p>Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II.</p> <p>This data set is for the gene <em>iscub</em> and is obtained from fixed zebrafish embryos, collected at the developmental stages oblong, sphere, dome, 30% epiboly, and 50% epiboly (indicated in the file names). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two independent experiments were performed (IF1, IF2 in the file name), for each experiment, two samples were prepared per stage and experiment (001, 002 in the file name).</p> <p>The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).</p>
IsoAnalyst: A System-Wide Stable Isotopic Labeling Aproach for Connecting Natural Products to Their Cognate Biosynthetic Gene Clusters
<p>Processed mass spectrometry data input used to develop and validate the IsoAnalyst program and the output from these analyses. The S_erythraea folder contains all MS input and output data for <em>Saccharopolyspora erythraea</em>. The Micromonospora_RL09050HVFA folder contains MS input and output data for <em>Micromonopora sp.</em> RL09050HVFA, as well as the full antiSMASH output for the <em>Micromonopora sp.</em> RL09050HVFA genome.</p> <p>Version 2 contains updated IsoAnalyst results for both organisms. </p>
Supporting data for the manuscript "Nerpa: a tool for discovering biosynthetic gene clusters of nonribosomal peptides"
<p>Preprocessed structures of nonribosomal peptides [NRPs] and genomic sequences (reference and representative genomes, biosynthetic gene clusters [BGCs]) used in the benchmark experiments in the Nerpa paper.</p> <p><strong>Files description</strong></p> <ul> <li><strong>mibig_nrp_bacteria_preprocessed.tar.gz</strong> contains the preprocessed dataset of 194 bacterial NRP BGCs from the MIBiG database.</li> <li><strong>mibig_nrp_bacteria_summary.tsv</strong> contains metadata for the MIBiG-NRP dataset.</li> <li><strong>bacterial_ref_and_repr_genomes_20210604_preprocessed.tar.gz</strong> contains the preprocessed dataset of 13,399 reference and representative bacterial genomes from the NCBI RefSeq database (retrieved on 2021/06/04).</li> <li><strong>bacterial_ref_and_repr_genomes_20210604_summary.txt</strong> contains metadata for the RefSeq dataset.</li> <li><strong>pnrpdb_preprocessed.info</strong> contains the Nerpa-preprocessed pNRPdb database, a database of 8,368 known and putative NRP structures.</li> <li><strong>pnrpdb_summary.tsv</strong> contains the pNRPdb database metadata.<br> </li> </ul>
Supplemental data for: Endophyte genomes support greater metabolic gene cluster diversity compared with non-endophytes in Trichoderma
<p><em>Trichoderma</em> is a cosmopolitan genus with diverse lifestyles and nutritional modes, including mycotrophy, saprophytism, and endophytism. Previous research has reported greater metabolic gene repertoires in endophytic fungal species compared to closely-related non-endophytes. However, the extent of this ecological trend and its underlying mechanisms are unclear. Some endophytic fungi may also be mycotrophs and have one or more mycoparasitism mechanisms. Mycotrophic endophytes are prominent in certain genera like <em>Trichoderma</em>, therefore, the mechanisms that enable these fungi to colonize both living plants and fungi may be the result of expanded metabolic gene repertoires. Our objective was to determine what, if any, genomic features are overrepresented in endophytic fungi genomes in order to undercover the genomic underpinning of the fungal endophytic lifestyle. Here we compared metabolic gene cluster and mycoparasitism gene diversity across a dataset of thirty-eight <em>Trichoderma</em> genomes representing the full breadth of environmental <em>Trichoderma</em>'s diverse lifestyles and nutritional modes. We generated four new <em>Trichoderma endophyticum</em> genomes to improve the sampling of endophytic isolates from this genus. As predicted, endophytic <em>Trichoderma</em> genomes contained, on average, more total biosynthetic and degradative gene clusters than non-endophytic isolates, suggesting that the ability to create/modify a diversity of metabolites potential is beneficial or necessary to the endophytic fungi. Still, once the phylogenetic signal was taken into consideration, no particular class of metabolic gene cluster was independently associated with the <em>Trichoderma</em> endophytic lifestyle. Several mycoparasitism genes, but no chitinase genes, were associated with endophytic <em>Trichoderma</em> genomes. Most genomic differences between <em>Trichoderma</em> lifestyles and nutritional modes are difficult to disentangle from phylogenetic divergences among species, suggesting that <em>Trichoderma</em> genomes may be particularly well-equipped for lifestyle plasticity. We also consider the role of endophytism in diversifying secondary metabolism after identifying the horizontal transfer of the ergot alkaloid gene cluster to <em>Trichoderma</em>.</p>
Phylogenomics of the psychoactive mushroom genus Psilocybe and evolution of the psilocybin biosynthetic gene cluster
<p>Psychoactive mushrooms in the genus <em>Psilocybe</em> have immense cultural value and have been used for centuries in Mesoamerica. Despite a recent surge in interest in these mushrooms due to emerging evidence that psilocybin, the main psychoactive compound, is a promising therapeutic for a variety of mental illnesses, their phylogeny and taxonomy remain substantially incomplete. Moreover, the recent elucidation of the psilocybin biosynthetic gene cluster is known for only five species of <em>Psilocybe</em>, four of which belong to only one of two major clades. We set out to improve the phylogeny for <em>Psilocybe</em> using shotgun sequencing of 71 fungarium specimens, including 23 types, and conducting phylogenomic analysis using 2,983 single-copy gene families to generate a fully supported phylogeny. Molecular clock analysis suggests the stem lineage arose ~67 mya and diversified ~56 mya. We also show that psilocybin biosynthesis first arose in <em>Psilocybe</em>, with 4–5 possible horizontal transfers to other mushrooms between 40 and 9mya. Moreover, predicted orthologs of the psilocybin biosynthetic genes revealed two distinct gene orders within the cluster that corresponds to a deep split within the genus, possibly consistent with the independent acquisition of the cluster. By mining genomic data beyond markers for phylogenetic inference, we gained novel insights into the evolutionary origins of psilocybin biosynthesis that have implications for understanding the functional role of this powerful chemical and can inform translational applications for human well-being.</p>
Data from: A high-quality genome assembly of the tetraploid Teucrium chamaedrys unveils a recent whole genome duplication and a large biosynthetic gene cluster for diterpenoid metabolism
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Data for: Tomato root specialized metabolites evolved through gene duplication and regulatory divergence within a biosynthetic gene cluster
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Gene clustering and copy number variation in alkaloid metabolic pathways of opium poppy
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Data for: Endophyte genomes support greater metabolic gene cluster diversity compared with non-endophytes in Trichoderma
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Metagenomic bins and biosynthetic gene clusters in gut bacteria of turtle ants
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Phylogenomics of the psychoactive mushroom genus Psilocybe and evolution of the psilocybin biosynthetic gene cluster
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Ancient gene clusters govern the initiation of monoterpenoid indole alkaloid biosynthesis and C3 stereochemistry inversion
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Association of oligopaint FISH labeled super-enhancers and genes with polymerase clusters (raw data, SE1-SE6)
<p>This data set assesses the placement of different genomic regions relative to clusters formed by RNA polymerase II in zebrafish embryos. RNA polymerase was labeled by immunofluorescence, genomic regions by oligopaint DNA fluorescence in-situ hybridization (FISH). Microscopy images were acquired by instant-SIM microscopy, and analyzed using MatLab scripts and the bioformats importer. This data set contains the raw image data as well as all further analysis scripts.</p> <p>Raw image data for super enhancers SE1 to SE6</p>
Association of oligopaint FISH labeled super-enhancers and genes with polymerase clusters (raw data, genes)
<p>This data set assesses the placement of different genomic regions relative to clusters formed by RNA polymerase II in zebrafish embryos. RNA polymerase was labeled by immunofluorescence, genomic regions by oligopaint DNA fluorescence in-situ hybridization (FISH). Microscopy images were acquired by instant-SIM microscopy, and analyzed using MatLab scripts and the bioformats importer. This data set contains the raw image data as well as all further analysis scripts.</p> <p>Raw image data for four genes: rnf19a, cdc25b, celf1, crsp7</p>
Association of oligopaint FISH labeled super-enhancers and genes with polymerase clusters (raw data, SE7-SE12)
<p>This data set assesses the placement of different genomic regions relative to clusters formed by RNA polymerase II in zebrafish embryos. RNA polymerase was labeled by immunofluorescence, genomic regions by oligopaint DNA fluorescence in-situ hybridization (FISH). Microscopy images were acquired by instant-SIM microscopy, and analyzed using MatLab scripts and the bioformats importer. This data set contains the raw image data as well as all further analysis scripts.</p> <p>Raw image data for super enhancers SE7 to SE12</p>
Association of oligopaint FISH labeled super-enhancers and genes with polymerase clusters
<p>This data set assesses the placement of different genomic regions relative to clusters formed by RNA polymerase II in zebrafish embryos. RNA polymerase was labeled by immunofluorescence, genomic regions by oligopaint DNA fluorescence in-situ hybridization (FISH). Microscopy images were acquired by instant-SIM microscopy, and analyzed using MatLab scripts and the bioformats importer. This data set contains the raw image data as well as all further analysis scripts.</p>
Data from: Comprehensive identification and clustering of CLV3/ESR-related (CLE) genes in plants finds groups with potentially shared function
CLV3/ESR (CLE) proteins are important signaling peptides in plants. The short CLE peptide (12–13 amino acids) is cleaved from a larger pre-propeptide and functions as an extracellular ligand. The CLE family is large and has resisted attempts at classification because the CLE domain is too short for reliable phylogenetic analysis and the pre-propeptide is too variable. We used a model-based search for CLE domains from 57 plant genomes and used the entire pre-propeptide for comprehensive clustering analysis. In total, 1628 CLE genes were identified in land plants, with none recognizable from green algae. These CLEs form 12 groups within which CLE domains are largely conserved and pre-propeptides can be aligned. Most clusters contain sequences from monocots, eudicots and Amborella trichopoda, with sequences from Picea abies, Selaginella moellendorffii and Physcomitrella patens scattered in some clusters. We easily identified previously known clusters involved in vascular differentiation and nodulation. In addition, we found a number of discrete groups whose function remains poorly characterized. Available data indicate that CLE proteins within a cluster are likely to share function, whereas those from different clusters play at least partially different roles. Our analysis provides a foundation for future evolutionary and functional studies.
Data from: A universal probe set for targeted sequencing of 353 nuclear genes from any flowering plant designed using k-medoids clustering
Sequencing of target-enriched libraries is an efficient and cost-effective method for obtaining DNA sequence data from hundreds of nuclear loci for phylogeny reconstruction. Much of the cost of developing targeted sequencing approaches is associated with the generation of preliminary data needed for the identification of orthologous loci for probe design. In plants, identifying orthologous loci has proven difficult due to a large number of whole-genome duplication events, especially in the angiosperms (flowering plants). We used multiple sequence alignments from over 600 angiosperms for 353 putatively single-copy protein-coding genes identified by the One Thousand Plant Transcriptomes Initiative to design a set of targeted sequencing probes for phylogenetic studies of any angiosperm group. To maximize the phylogenetic potential of the probes while minimizing the cost of production, we introduce a k-medoids clustering approach to identify the minimum number of sequences necessary to represent each coding sequence in the final probe set. Using this method, five to 15 representative sequences were selected per orthologous locus, representing the sequence diversity of angiosperms more efficiently than if probes were designed using available sequenced genomes alone. To test our approximately 80,000 probes, we hybridized libraries from 42 species spanning all higher-order groups of angiosperms, with a focus on taxa not present in the sequence alignments used to design the probes. Out of a possible 353 coding sequences, we recovered an average of 283 per species and at least 100 in all species. Differences among taxa in sequence recovery could not be explained by relatedness to the representative taxa selected for probe design, suggesting that there is no phylogenetic bias in the probe set. Our probe set, which targeted 260 kbp of coding sequence, achieved a median recovery of 137 kbp per taxon in coding regions, a maximum recovery of 250 kbp, and an additional median of 212 kbp per taxon in flanking non-coding regions across all species. These results suggest that the Angiosperms353 probe set described here is effective for any group of flowering plants and would be useful for phylogenetic studies from the species level to higher-order groups, including the entire angiosperm clade itself.
S1_The_differentially_expressed_genes_enriched_in_GO_clusters
<p>This is supporting information to the article <em>Downregulation of ammonium uptake improves the growth and tolerance of Kluyveromyces marxianus at high temperature</em>, which has been submitted to <em>MicrobiologyOpen</em>.</p>
Genome sequences of Rhizopogon roseolus, Mariannaea elegans, Myrothecium verrucaria, and Sphaerostilbella broomeana and the identification of biosynthetic gene clusters for fungal peptide natural products
<p>Data to accompany the paper, detailed in manifest.txt</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.