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144 results for “genetic modifiers”
Monitoring and traceability of genetically modified soybean event GTS 40-3-2 during soybean protein concentrate and isolate preparation
To evaluate DNA fragmentation and GMO quantification during soybean protein concentrate and isolate preparation, genetically modified soybean event GTS 40-3-2 (RRS) was blended with conventional soybeans at mass percentages of 0.9%, 2%, 3%, 5%, and 10%. Qualitative PCR and real-time PCR were used to monitor the taxon-specific lectin and exogenous cp4 epsps target levels in all of the main products and by-products, which has practical significance for RRS labelling threshold and traceability. Along the preparation chain, the majority of DNA was distributed in main products, and the DNA degradation was noticed. From a holistic perspective, the lectin target degraded more than cp4 epsps target during both of the two soybean proteins preparations. Therefore, the transgenic contents in the final protein products were higher than the actual mass percentages of RRS in raw materials. Our results are beneficial to the improvement of GMO labelling legislation and the protection of consumer rights.
Data from: Hybridization between genetically modified Atlantic salmon and wild brown trout reveals novel ecological interactions
Interspecific hybridization is a route for transgenes from genetically modified (GM) animals to invade wild populations, yet the ecological effects and potential risks that may emerge from such hybridization are unknown. Through experimental crosses, we demonstrate transmission of a growth hormone transgene via hybridization between a candidate for commercial aquaculture production, GM Atlantic salmon (Salmo salar), and closely related wild brown trout (S. trutta). Transgenic hybrids were viable and grew more rapidly than transgenic salmon and other non-transgenic crosses in hatchery-like conditions. In stream mesocosms designed to more closely emulate natural conditions, transgenic hybrids appeared to express competitive dominance and suppressed the growth of transgenic and non-transgenic (wild-type) salmon by 82% and 54%, respectively. To the best of our knowledge, this is the first demonstration of environmental impacts of hybridization between a GM animal and a closely related species. These results provide empirical evidence of the first steps towards introgression of foreign transgenes into the genomes of new species and contribute to the growing evidence that transgenic animals have complex and context-specific interactions with wild populations. We suggest that interspecific hybridization be explicitly considered when assessing the environmental consequences should transgenic animals escape to nature.
Data from: Forest trees in human modified landscapes: ecological and genetic drivers of recruitment failure in Dysoxylum malabaricum (Meliaceae)
Tropical agro-forest landscapes are global priority areas for biodiversity conservation. Little is known about the ability of these landscapes to sustain large late successional forest trees upon which much forest biodiversity depends. These landscapes are subject to fragmentation and additional habitat degradation which may limit tree recruitment and thus compromise numerous ecosystem services including carbon storage and timber production. Dysoxylum malabaricum is a large canopy tree species in the Meliaceae, a family including many important tropical timber trees. This species is found in highly fragmented forest patches within a complex agro-forest landscape of the Western Ghats biodiversity hot spot, South India. In this paper we combined a molecular assessment of inbreeding with ecological and demographic data to explore the multiple threats to recruitment of this tree species. An evaluation of inbreeding, using eleven microsatellite loci in 297 nursery-reared seedlings collected form low and high density forest patches embedded in an agro-forest matrix, shows that mating between related individuals in low density patches leads to reduced seedling performance. By quantifying habitat degradation and tree recruitment within these forest patches we show that increasing canopy openness and the increased abundance of pioneer tree species lead to a general decline in the suitability of forest patches for the recruitment of D. malabaricum. We conclude that elevated inbreeding due to reduced adult tree density coupled with increased degradation of forest patches, limit the recruitment of this rare late successional tree species. Management strategies which maintain canopy cover and enhance local densities of adult trees in agro-forest mosaics will be required to ensure D. malabaricum persists in these landscapes. Our study highlights the need for a holistic understanding of the incipient processes that threaten populations of many important and rare tropical tree species in human dominated agro-forest landscapes.
Data from: Evaluating otter reintroduction outcomes using genetic spatial capture-recapture modified for dendritic networks
<p>River otters (Lontra canadensis) were extirpated from New Mexico by the 1950s. A limited reintroduction occurred during 2008–2010 in which 33 otters sourced from Washington (WA) were translocated to the Upper Rio Grande Basin (URG) of New Mexico. We conducted a noninvasive genetic capture-recapture survey during the winter of 2018 by collecting fecal DNA samples from river otter scats found at latrines in the URG dendritic network of perennial waterways. Our objectives were to: 1) estimate genetic diversity and effective population size; 2) genetic divergence from the WA source population and potential connectivity with regionally proximal populations; 3) spatially explicit population density and size; and 4) population growth rate since the founder event. Between February and April 2018, we collected 1,184 fecal DNA samples from 622 individual scats at 20 latrines; genotyping was attempted at 10 otter-specific microsatellite loci for a subsample of 543 samples. A bottlenecking founder effect was strongly supported, which, combined with genetic drift, reduced genetic diversity and effective population size by 20–26% and 106–170%, respectively, compared with the WA source population. Estimated population density from spatial capture-recapture models was 0.23–0.28 otter/km of waterway, or 1 otter/3.57–4.35 km of waterway, corresponding to a total population size of 83–100 otters across 359 km of the perennial dendritic network from La Mesilla, New Mexico to Alamosa National Wildlife Refuge, Colorado. Estimated average annual population growth rate since the founder event was 1.12–1.15/year. Despite successful population establishment, the URG river otter population remains small, is genetically degraded, and does not yet meet the criteria for long-term reintroduction success. Projections suggested that the population could reach the recommended minimum viable population size of ≥400 otters by the years 2030–2033, though sufficient habitat may not exist in the URG Basin to support that many otters. </p>
Data from: Detection of airborne genetically modified maize pollen by real-time PCR
The cultivation of genetically modified (GM) crops has raised in the European Union and other parts of the world numerous concerns about their environmental and economic impact. Especially outcrossing of genetically modified organisms (GMO) was from the beginning a critical issue as airborne pollen has been considered an important way of GMO dispersal. Here, we investigate the use of airborne pollen sampling combined with microscopic analysis and molecular PCR analysis as an approach to monitor GM maize cultivations in a specific area. Field trial experiments in the European Union and South America demonstrated the applicability of the approach under different climate conditions, in rural and semi-urban environment, even at very low levels of airborne pollen. The study documents in detail the sampling of GM pollen, sample DNA extraction and real-time PCR analysis. Our results suggest that this ''GM pollen monitoring by bioaerosol sampling and PCR screening" approach might represent an aid in managing the co-cultivation of GM and non-GM crops, especially in the surveillance of GM-free areas, centres of origin, and natural reserves.
Genetic Modifiers of Cystic Fibrosis: Sibling Study
ClinicalTrials.gov study NCT00037778. IPD Sharing: Not stated. Countries: 1. Publications: 4.
An Antiretroviral Treatment Interruption (ATI) Study to Evaluate the Impact of Genetically Modified Autologous Cells (AGT103-T) to Suppress Human Immunodeficiency Virus Replication in the Absence of A
ClinicalTrials.gov study NCT05540964. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
A Study of a Potential Disease Modifying Treatment in Individuals at Risk for or With a Type of Early Onset AD Caused by a Genetic Mutation
ClinicalTrials.gov study NCT06647498. IPD Sharing: YES. Countries: 14. Publications: 16.
Infusion of Genetically Modified T Cell for Post Transplant Patients With Relapsed Disease
ClinicalTrials.gov study NCT00871702. IPD Sharing: Not stated. Countries: 1. Publications: 16.
Safety and Survival of Genetically Modified White Blood Cells in HIV-infected Twins The Gemini Study
ClinicalTrials.gov study NCT04799483. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Study of the Role of Genetic Modifiers in Hemoglobinopathies
ClinicalTrials.gov study NCT05799118. IPD Sharing: Not stated. Countries: 16. Publications: 1.
Treatment of Patients With Cancer With Genetically Modified Salmonella Typhimurium Bacteria
ClinicalTrials.gov study NCT00004988. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Intra-pleural Administration of GL-ONC1, a Genetically Modified Vaccinia Virus, in Patients With Malignant Pleural Effusion: Primary, Metastases and Mesothelioma
ClinicalTrials.gov study NCT01766739. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Genetic Modifiers of Cystic Fibrosis (CF) Liver Disease
ClinicalTrials.gov study NCT00804583. IPD Sharing: Not stated. Countries: 1. Publications: 9.
Genetically Modified Lymphocytes to Treat HIV-Infected Identical Twins - Study Modifications
ClinicalTrials.gov study NCT00001409. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Genetically Modified T-cell Infusion Following Peripheral Blood Stem Cell Transplant in Treating Patients With Recurrent or High-Risk Non-Hodgkin Lymphoma
ClinicalTrials.gov study NCT01815749. IPD Sharing: Not stated. Countries: 1. Publications: 2.
A Study of Potential Disease Modifying Treatments in Individuals at Risk for or With a Type of Early Onset AD Caused by a Genetic Mutation
ClinicalTrials.gov study NCT05552157. IPD Sharing: YES. Countries: 14. Publications: 13.
Safety and Survival of Genetically Modified White Blood Cells in HIV-Infected Persons - A Study in Identical Twin Pairs
ClinicalTrials.gov study NCT00001353. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Evaluating Genetic Modifiers of Cutaneous Neurofibromas in Adults With Neurofibromatosis Type 1
ClinicalTrials.gov study NCT04941027. IPD Sharing: YES. Countries: 1. Publications: 0.
Evaluation of Clinical and Genetic Modifiers of Long-term Survival in Heart Failure
ClinicalTrials.gov study NCT03461107. IPD Sharing: Not stated. Countries: 1. Publications: 7.
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Allen Brain Atlas
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.