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32 results for “gut content analysis”
Data from: The effect of plant identity and the level of plant decay on molecular gut content analysis in a herbivorous soil insect
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Figures 28-31 from: Klimaszewski J, Morency M, Labrie P, Seguin A, Langor D, Work T, Bourdon C, Thiffault E, Pare D, Newton A (2013) Molecular and microscopic analysis of the gut contents of abundant rove beetle species (Coleoptera, Staphylinidae) in the boreal balsam fir forest of Quebec, Canada. ZooKeys 353: 1-24. https://doi.org/10.3897/zookeys.353.5991
Figures 28-31 - Images of hindgut content of the following rove beetle species: 28–30 Tachinus fumipennis (Say) 31 Tachinus quebecensis Robert.
Figures 12-15 from: Klimaszewski J, Morency M, Labrie P, Seguin A, Langor D, Work T, Bourdon C, Thiffault E, Pare D, Newton A (2013) Molecular and microscopic analysis of the gut contents of abundant rove beetle species (Coleoptera, Staphylinidae) in the boreal balsam fir forest of Quebec, Canada. ZooKeys 353: 1-24. https://doi.org/10.3897/zookeys.353.5991
Figures 12-15 - Images of hindgut content of the following rove beetle species: 12–13 Atheta capsularis Klimaszewski 14–15 Atheta klagesi Bernhauer.
Figures 8-11 from: Klimaszewski J, Morency M, Labrie P, Seguin A, Langor D, Work T, Bourdon C, Thiffault E, Pare D, Newton A (2013) Molecular and microscopic analysis of the gut contents of abundant rove beetle species (Coleoptera, Staphylinidae) in the boreal balsam fir forest of Quebec, Canada. ZooKeys 353: 1-24. https://doi.org/10.3897/zookeys.353.5991
Figures 8-11 - Body images of rove beetles in dorsal view: 8 Tachinus frigidus Erichson 9 Tachinus fumipennis (Say) 10 Tachinus quebecensis Robert 11 Pseudopsis subulata Herman.
Figures 2-7 from: Klimaszewski J, Morency M, Labrie P, Seguin A, Langor D, Work T, Bourdon C, Thiffault E, Pare D, Newton A (2013) Molecular and microscopic analysis of the gut contents of abundant rove beetle species (Coleoptera, Staphylinidae) in the boreal balsam fir forest of Quebec, Canada. ZooKeys 353: 1-24. https://doi.org/10.3897/zookeys.353.5991
Figures 2-7 - Body images of rove beetles in dorsal view: 2 Atheta capsularis Klimaszewski 3 Atheta klagesi Bernhauer 4 Oxypoda grandipennis (Casey) 5 Bryophacis smetanai Campbell 6 Ischnosoma longicorne (Mäklin) [previously cited as synonymous Ischnosoma fimbriatum Campbell] 7 Mycetoporus montanus Luze [previously cited as synonymous Mycetoporus rugosus Hatch].
Figures 20-23 from: Klimaszewski J, Morency M, Labrie P, Seguin A, Langor D, Work T, Bourdon C, Thiffault E, Pare D, Newton A (2013) Molecular and microscopic analysis of the gut contents of abundant rove beetle species (Coleoptera, Staphylinidae) in the boreal balsam fir forest of Quebec, Canada. ZooKeys 353: 1-24. https://doi.org/10.3897/zookeys.353.5991
Figures 20-23 - Images of hindgut content of the following rove beetle species: 20 Ischnosoma longicorne (Mäklin) 21–22 Mycetoporus montanus Luze 23 Tachinus frigidus Erichson.
Figures 32-35 from: Klimaszewski J, Morency M, Labrie P, Seguin A, Langor D, Work T, Bourdon C, Thiffault E, Pare D, Newton A (2013) Molecular and microscopic analysis of the gut contents of abundant rove beetle species (Coleoptera, Staphylinidae) in the boreal balsam fir forest of Quebec, Canada. ZooKeys 353: 1-24. https://doi.org/10.3897/zookeys.353.5991
Figures 32-35 - Images of hindgut content of the following rove beetle species: 32–33 Tachinus quebecensis Robert 34–35 Pseudopsis subulata Herman.
Figures 24-27 from: Klimaszewski J, Morency M, Labrie P, Seguin A, Langor D, Work T, Bourdon C, Thiffault E, Pare D, Newton A (2013) Molecular and microscopic analysis of the gut contents of abundant rove beetle species (Coleoptera, Staphylinidae) in the boreal balsam fir forest of Quebec, Canada. ZooKeys 353: 1-24. https://doi.org/10.3897/zookeys.353.5991
Figures 24-27 - Images of hindgut content of the following rove beetle species: 24–26 Tachinus frigidus Erichson 27 Tachinus fumipennis (Say).
Figures 16-19 from: Klimaszewski J, Morency M, Labrie P, Seguin A, Langor D, Work T, Bourdon C, Thiffault E, Pare D, Newton A (2013) Molecular and microscopic analysis of the gut contents of abundant rove beetle species (Coleoptera, Staphylinidae) in the boreal balsam fir forest of Quebec, Canada. ZooKeys 353: 1-24. https://doi.org/10.3897/zookeys.353.5991
Figures 16-19 - Images of hindgut content of the following rove beetle species: 16 Oxypoda grandipennis (Casey) 17–18 Bryophacis smetanai Campbell 19 Ischnosoma longicorne (Mäklin).
Data from: Multiplex PCR targeting lineage specific SNPs ‐ A highly efficient and simple approach to block out predator sequences in molecular gut content analysis
Background: Food webs form the basis of biological communities, though empirical research has been hindered by difficulties in quantifying interactions. Metabarcoding from predator gut content extractions with universal primers promises to provide simple and rapid insights into food web interactions. However, the highly overabundant predator DNA often completely outcompetes that of the digested prey DNA during PCR, impeding the ability to assess the abundance and diversity of prey items. Methods: Focusing on the issue of overabundance of predator DNA amplified by a commonly used COI primer pair, we use predator lineage-specific SNPs at the 3'-end of PCR primers to selectively block out predators from amplification. While this approach largely prevents predator amplification, it retains high taxonomic versatility for prey lineages. We introduce a novel multilocus assay, targeting four nuclear and mitochondrial rDNA markers and test our approach in a diverse set of spiders from 12 families. We estimate the recovered prey DNA proportions and compare the taxonomic composition of prey communities between markers. Using a feeding experiment, we also explore recovery of prey DNA over time. Results: While commonly used COI primers yield low and very unpredictable amounts of prey DNA, our assay allows for a considerable and consistent prey enrichment across all tested species. The recovered prey's taxonomic composition is comparable between markers and supports results acquired by COI. The new marker set can be amplified in a simple multiplex PCR, considerably reducing the necessary workload. Conclusions: Our multi-locus approach allows the generation of an unprecedented amount of prey data at low cost and effort. Lineage specific PCR is taxonomically versatile and could readily be adapted to any prey-predator interaction, opening up the opportunity for community-wide studies on food web interactions.
Figure 1 from: Klimaszewski J, Morency M, Labrie P, Seguin A, Langor D, Work T, Bourdon C, Thiffault E, Pare D, Newton A (2013) Molecular and microscopic analysis of the gut contents of abundant rove beetle species (Coleoptera, Staphylinidae) in the boreal balsam fir forest of Quebec, Canada. ZooKeys 353: 1-24. https://doi.org/10.3897/zookeys.353.5991
Figure 1 - Map of ribosomal RNA genes and ITS regions.
Data from: Multiplex PCR targeting lineage specific SNPs ‐ A highly efficient and simple approach to block out predator sequences in molecular gut content analysis
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