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57 results for “host detection”
Data from: Detecting ancient co-dispersals and host shifts by double dating of host and parasite phylogenies: application in proctophyllodid feather mites associated with passerine birds
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Data from: Molecular detection of trophic links in a complex insect host-parasitoid food web
Previously, host-parasitoid links have been unveiled almost exclusively by time-intensive rearing, while molecular methods were used only in simple agricultural host-parasitoid systems in the form of species specific primers. Here we present a general method for molecular detection of these links applied to a complex caterpillar-parasitoid food web from tropical rainforest of Papua New Guinea. We DNA barcoded hosts, parasitoids and their tissue remnants and matched the sequences to our extensive library of local species. We were thus able to match 87% of host sequences and 36% of parasitoid sequences to species, and infer subfamily or family in almost all cases. Our analysis affirmed 93 hitherto unknown trophic links between 37 host species from a wide range of Lepidoptera families and 46 parasitoid species from Hymenoptera and Diptera by identifying DNA sequences for both the host and the parasitoid involved in the interaction. Molecular detection proved especially useful in cases where distinguishing host species in caterpillar stage was difficult morphologically, or when the caterpillar died during rearing. We have even detected a case of extreme parasitoid specialization in a pair of Choreutis species which do not differ in caterpillar morphology and ecology. Using the molecular approach outlined here leads to better understanding of parasitoid host specificity, opens new possibilities for rapid surveys of food web structure and allows inference of species associations not already anticipated.
FIGURE. Phylogenetic tree of specimens on Poaceae and related host plants constructed by MP method based on ITS+28S regions of rDNA. Bootstrap values of MP and ML are followed by the Bayesian posterior probabilities (Bpp) on the nodes in the topology. Asterisk (*) represents bootstrap values or Bpp less than 50% in the topology. Sample data are shown with voucher specimen number or GenBank accession number, and host plant. Sequence data determined in this study are shown in color. Teliospore shapes are shown in each clade detected, and new species are shown by asterisk (*) on clades. 0, I: Spermogonial and aecial host genus. Asterisk (*) on host plants: Spermogonial and aecial host plants. in Phylogenetic approach for identification and life cycles of Puccinia (Pucciniaceae) species on Poaceae from northeastern China
FIGURE. Phylogenetic tree of specimens on Poaceae and related host plants constructed by MP method based on ITS+28S regions of rDNA. Bootstrap values of MP and ML are followed by the Bayesian posterior probabilities (Bpp) on the nodes in the topology. Asterisk (*) represents bootstrap values or Bpp less than 50% in the topology. Sample data are shown with voucher specimen number or GenBank accession number, and host plant. Sequence data determined in this study are shown in color. Teliospore shapes are shown in each clade detected, and new species are shown by asterisk (*) on clades. 0, I: Spermogonial and aecial host genus. Asterisk (*) on host plants: Spermogonial and aecial host plants.
Fig. 1 in New Turfgrass Host Associations and Regional Detections for the Flea Beetle Chaetocnema minuta Melsheimer (Coleoptera: Chrysomelidae) in the Southwestern United States
Fig. 1. Adult and larva of Chaetocnema minuta isolated from damaged creeping bentgrass in southern Utah (photo: A. Van DYke).
Data from: Using a comprehensive DNA barcode library to detect novel egg and larval host plant associations in a Cephaloleia Rolled-leaf Beetle (Coleoptera: Chrysomelidae)
To fully understand the ecology and evolution of plant-herbivore interactions, information regarding the life history of both immature and adult insect stages is essential. However, most knowledge of plant-herbivore associations is derived from observations of adults. One reason for this bias is that species identification of immature stages is usually challenging. DNA barcodes can be used to identify immature stages to the species-level. This technique compares short sequences of the appropriate DNA barcode loci (e.g., mitochondrial COI gene for insects) of an unidentified specimen to a known DNA barcode library. The accuracy of DNA-based identifications depends on the comprehensiveness of the DNA barcode library. We generated a comprehensive DNA barcode library for a community of Rolled-leaf Beetles (Coleoptera: Chrysomelidae) in a premontane tropical forest in Costa Rica. The DNA barcode COI accurately identified all beetle species included in this study. Using this DNA barcode library, we identified eggs and larvae of Cephaloleia histrionica Baly with 100% confidence. This new record of C. histrionica is unique in that this species completes its life cycle on a bromeliad, whereas most Cephaloleia species are associated with plants from the order Zingiberales. The life cycle, diet breadth, immature stages, and sexual dimorphism are described for C. histrionica.
Figure 4 in Distal leg morphology, subgenual organs and host detection in Stephanidae (Insecta, Hymenoptera)
Figure 4. Megischus spp., female. (A) Hind leg, median view. (B) Hind tibia proximally at level of subgenual organ, lateral view. (C) Hind tarsus, tarsomeres 1–3 (different species from the one illustrated in Figure 4A).
Figure 1 in Distal leg morphology, subgenual organs and host detection in Stephanidae (Insecta, Hymenoptera)
Figure 1. Schlettererius cinctipes, female, foreleg and mid-leg. (A) Fore leg, median view. (B) Basal part of fore tibia, median view, with dome concealing subgenual organ. (C) Fore tarsus, tarsomeres 2–5. (D) Mid leg, lateral view.
Figure 6 in Distal leg morphology, subgenual organs and host detection in Stephanidae (Insecta, Hymenoptera)
Figure 6. Parastephanellus sp., female ovipositing. Redrawn after Rodd (1951, figures 1 and 3). Early (top) and late (bottom) stage. X indicates the point of entry of the ovipositor into the wood, triangles the position of the subgenual organs in the different legs.
Detection and Characterization of Host Defense Defects
ClinicalTrials.gov study NCT00001355. IPD Sharing: YES. Countries: 1. Publications: 3.
Data from: Using a comprehensive DNA barcode library to detect novel egg and larval host plant associations in a Cephaloleia Rolled-leaf Beetle (Coleoptera: Chrysomelidae)
Open the record for dataset details and reuse information.
Data from: Molecular detection of trophic links in a complex insect host-parasitoid food web
Open the record for dataset details and reuse information.
Figure 3 in New host detection of the parasitic mite, Erythraeus pistacicus (Trombidiformes: Erythraeidae) from Iran and indication of possible infection with bacterial symbionts
Figure 3. Phylogenetic tree based on wsp sequences of Wolbachia, constructed by a neighbor-joining procedure. Wolbachia strains are depicted by the host name. The accession numbers are shown after the host name. Numbers on the nodes indicate bootstrap percent confidence values.
Fig. 1 in Detection of haemosporidian parasites in wild and domestic birds in northern and central provinces of Iran: Introduction of new lineages and hosts
Fig. 1. Diversity of reported lineages in the same hosts in the current study. Frequency and diversity of available lineages of each continent are given from A to C. Plasmodium (A), Haemoproteus (B) and Leucocytozoon (C). The Y axis is the geographic locations (continents) and the X axis is the frequency of known lineages around the world.
Detection of Graft Versus Host Disease With [18F]F-AraG
ClinicalTrials.gov study NCT03367962. IPD Sharing: NO. Countries: 1. Publications: 0.
Circulating Cell-Free RNA in Blood as a Host Response Biomarker for the Detection of Tuberculosis [validation_data]
GEO Series GSE255074. Homo sapiens. 60 samples. Type: Expression profiling by high throughput sequencing.
Systematic detection of host pathways universally inhibited by Plasmodium yoelii parasites for immune intervention
GEO Series GSE114718. Mus musculus. 102 samples. Type: Expression profiling by array.
Integrative profiling of early host chromatin accessibility responses in human neutrophils with sensitive pathogen detection [RNA-seq]
GEO Series GSE153521. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
Host transcripts detected in exosomes derived from M.tb infected murine RAW264.7 macrophages
GEO Series GSE54419. Mus musculus. 12 samples. Type: Expression profiling by array.
Integrative profiling of early host chromatin accessibility responses in human neutrophils with sensitive pathogen detection [ATAC-seq]
GEO Series GSE153520. Homo sapiens. 76 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Circulating Cell-Free RNA in Blood as a Host Response Biomarker for the Detection of Tuberculosis [training_data]
GEO Series GSE255071. Homo sapiens. 130 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.