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Fig. 5 in Methods to separate Lobesia botrana (Lepidoptera: Tortricidae) males from females for the implementation of sterile insect-inherited sterility technique control tactics
Fig. 5. The percentages of male and female Lobesia botrana adults that emerged from the pupal stage on each consecutive d afer the onset of adult emergence.
Fig. 6 in Methods to separate Lobesia botrana (Lepidoptera: Tortricidae) males from females for the implementation of sterile insect-inherited sterility technique control tactics
Fig. 6. Distribution of Lobesia botrana male and female pupae based on the overall lengths of the pupae.
Fig. 3. R, G and B in Methods to separate Lobesia botrana (Lepidoptera: Tortricidae) males from females for the implementation of sterile insect-inherited sterility technique control tactics
Fig. 3. R, G and B color values of male and female Lobesia botrana larvae during the wandering phase. A. Average R, G and B color values of wandering male and female larvae, and B. Percentages of wandering red, green and blue larvae in relation to the R, G and B values as a proportion of total male and female larvae. * P = 0.001, Tukey α = 0.05.
Fig. 1 in Methods to separate Lobesia botrana (Lepidoptera: Tortricidae) males from females for the implementation of sterile insect-inherited sterility technique control tactics
Fig. 1. Lobesia botrana pupae indicating the number of abdominal segments in (A) male (4 segments), and (B) female pupae (3 segments) as measured from the abdomen tip to the wing's point, and (C) distance between the head and the abdomen tip as a measurement of pupal length.
Fig. 4 in Methods to separate Lobesia botrana (Lepidoptera: Tortricidae) males from females for the implementation of sterile insect-inherited sterility technique control tactics
Fig. 4. The percentages of wandering Lobesia botrana larvae that departed from their food on each consecutive day of the wandering phase and that sub- sequently developed into either adult males or adult females.
Fig. 5 in Inherited sterility in Tuta absoluta (Lepidoptera: Gelechiidae): Pest population suppression and potential for combined use with a generalist predator
Fig. 5. Prey consumption in the choice test involving progeny of irradiated females. Number of T. absoluta eggs consumed per T. cucurbitaceus (mean ± SE) individual in 24 h from the following T. absoluta eggs crosses, ♀U × ƋU and ♀I × ƋU. The different letters above the 2 error bars indicates that there was a significant difference in the numbers of eggs consumed based on the crosses from which they had originated, even though the female parent had been irradiated in 1 of the crosses (paired t tests, P <0.05). U = untreated, I = irradiated.
Fig. 3 in Inherited sterility in Tuta absoluta (Lepidoptera: Gelechiidae): Pest population suppression and potential for combined use with a generalist predator
Fig. 3. Prey consumption in the no-choice test. Number of T. absoluta eggs consumed per T. cucurbitaceus (mean ± SE) individual in 24 h from following T. absoluta eggs crosses: ♀U × ƋU,♀U × ƋI or ♀I × ƋU.The same letter above the 3 error bars indicates that there were no significant differences in the numbers of eggs consumed based on the crosses from which they had originated (ANOVA, P> 0.05). U = untreated, I = irradiated.
Fig. 1 in Inherited sterility in Tuta absoluta (Lepidoptera: Gelechiidae): Pest population suppression and potential for combined use with a generalist predator
Fig. 1. Population suppression by irradiated individuals and their progeny over 12 weeks at a 10:1 irradiated:untreated ratio. a) Number of eggs/ cage, b) number of small larvae /cage and c) number of large larvae/cage (mean ± SE). Only 1 release of irradiated moths was made, i.e., at the beginning of the experiment. For details see text on Experiment 1.
Fig. 4 in Inherited sterility in Tuta absoluta (Lepidoptera: Gelechiidae): Pest population suppression and potential for combined use with a generalist predator
Fig. 4. Prey consumption in the choice test involving progeny of irradiated males. Number of T. absoluta eggs from following T. absoluta eggs crosses, ♀U × ƋU and ♀U × ƋI,consumed per T. cucurbitaceus (mean ± SE) individual in 24 h. The same letter above the 2 error bars indicates that there was no significant difference in the numbers of eggs consumed based on the crosses from which they had originated, even though the male parent had been irradiated in 1 of the crosses (paired t tests, P> 0.05). U = untreated, I = irradiated.
Fig. 2 in Inherited sterility in Tuta absoluta (Lepidoptera: Gelechiidae): Pest population suppression and potential for combined use with a generalist predator
Fig. 2. Population suppression by irradiated individuals and their progeny over 12 weeks at a 15:1 irradiated:untreated ratio. a) Number of eggs/cage, b) number of small larvae /cage and c) number of large larvae/cage (mean ± SE). Only 1 release of irradiated moths was made, i.e., at the beginning of the experiment. For details see text on Experiment 2.
Fig. 2. Chromatograph comparing original and redesigned primers for Ccmic3 in Inheritance of fifeen microsatellite loci in Ceratitis capitata (Diptera: Tephritidae)
Fig. 2. Chromatograph comparing original and redesigned primers for Ccmic3 on sample A1-F1-07, Family A1. Both reactions were run simultaneously on the same fragment analysis plate using the same PCR conditions, DNA concentrations, and dilution factor. a) Chromatograph of progeny exhibiting an allele call of 74/74. Parents are 74/76 and 76/76. The observed 76 bp peak was considered to be weak. Cloning and sequencing confirmed the existence of this 76 bp fragment. b) Chromatograph of the same progeny as in Fig. 2a now exhibiting an allele call of 72/74 afer primer modification. Parents are now 72/74 and 74/74. The observed 74 bp peak is more pronounced compared to the previous 76 bp call. The intensity of the 74 bp peak also increased while the other 3 visible peaks decreased. This suggests an increase in adenylation has occurred.
Fig. 1 in Inheritance of fifeen microsatellite loci in Ceratitis capitata (Diptera: Tephritidae)
Fig. 1. Sequence alignment of Ccmic3 with original primer design. Top two sequences are genotypes of 74 bp allele and bottom two sequences are genotypes of the 76 bp allele. One extra thymine residue on the 5' end appeared in 8 of the 8 clones for this allele leading to difficulties in scoring this locus. Redesign of the forward primer by adding the extra guanines provided better resolution in scoring. Lower case sequence represents the Topo 2.1 vector just past the EcoRI in the multiple cloning site.
Fig. 3 in Inheritance of fifeen microsatellite loci in Ceratitis capitata (Diptera: Tephritidae)
Fig. 3. Comparing artifactual bands to actual bands to determine allele as observed at the Ccmic25 locus. a) Chromatograph of sample exhibiting an artifactual band can be observed at the 136 bp peak. The geometric shape and configuration is not consistent with those of microsatellites. b) This chromatograph shows the characteristic high peak followed by a low peak expected when making a call around the 137 bp peak. To the lef of both peaks are very low broad bands, commonly observed during analysis. In homozygote individuals, these peaks may extend higher and resemble an artifactual band similar to what was observed in Fig. 3a.
Nanopore deep sequencing as a tool to characterize and quantify aberrant splicing caused by variants in inherited retinal dystrophy genes
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Social network inheritance and differentiation in wild baboons
<p>Immatures' social development may be fundamental to understanding important biological processes, such as social information transmission through groups, that can vary with age and sex. Our aim was to determine how social networks change with age and differ between sexes in wild immature baboons, group-living primates that readily learn socially. Our results show that immature baboons inherited their mothers' networks and differentiated from them as they aged, increasing their association with partners of similar age and the same sex. Males were less bonded to their matriline and became more peripheral with age compared to females. Our results may pave the way to further studies testing a new hypothetical framework: in female-philopatric societies, social information transmission may be constrained at the matrilineal level by age- and sex-driven social clustering.</p>
Supplementary material for: PhyloCoalSimulations: A simulator for network multispecies coalescent models, including a new extension for the inheritance of gene flow
<p>We consider the evolution of phylogenetic gene trees along phylogenetic species networks, according to the network multispecies coalescent process, and introduce a new network coalescent model with correlated inheritance of gene flow. This model generalizes two traditional versions of the network coalescent: with independent or common inheritance. At each reticulation, multiple lineages of a given locus are inherited from parental populations chosen at random, either independently across lineages, or with positive correlation according to a Dirichlet process. This process may account for locus-specific probabilities of inheritance, for example.</p> <p>We implemented the simulation of gene trees under these network coalescent models in the Julia package PhyloCoalSimulations, which depends on PhyloNetworks and its powerful network manipulation tools. Input species phylogenies can be read in extended Newick format, either in numbers of generations or in coalescent units. Simulated gene trees can be written in Newick format, and in a way that preserves information about their embedding within the species network. This embedding can be used for downstream purposes, such as to simulate species-specific processes like rate variation across species, or for other scenarios as illustrated in this note. This package should be useful for simulation studies and simulation-based inference methods. The software is available open source with documentation and a tutorial at <a href="https://github.com/cecileane/PhyloCoalSimulations.jl">https://github.com/cecileane/PhyloCoalSimulations.jl</a>.</p>
Data from: Distinct patterns of inheritance shape life-history traits in steelhead trout
<p>Life-history variation is the raw material of adaptation, and understanding its genetic and environmental underpinning is key to designing effective conservation strategies. We used large-scale genetic pedigree reconstruction of anadromous steelhead trout (<em>Oncorhynchus</em> <em>mykiss</em>) from the Russian River, California, USA to elucidate sex-specific patterns of life-history traits and their heritability. SNP data from adults returning from sea over a 14-year period were used to identify 13,474 parent-offspring trios. These pedigrees were used to determine age structure, size distributions, and family sizes for these fish, as well as to estimate the heritability of two key life-history traits, spawn date and age at maturity (first reproduction). Spawn date was highly heritable (h<sup>2</sup> = 0.73) and had a cross-sex genetic correlation near unity. We provide the first estimate of heritability for age at maturity in ocean-going fish from this species and found it to be high heritable (h<sup>2</sup> from 0.29–0.62, depending upon sex and calculation), with a much lower genetic correlation across sexes. We also evaluated genotypes at a migration-associated inversion polymorphism and found sex-specific correlations with age at maturity. The significant heritability of these two key reproductive traits in these imperiled fish, and their patterns of inheritance in the two sexes, is consistent with predictions of both natural and sexually antagonistic selection (sexes experience opposing selection pressures). This emphasizes the importance of anthropogenic factors, including hatchery practices and ecosystem modifications, in shaping the fitness of this species, thus providing important guidance for management and conservation efforts.</p>
Supplementary material for: PhyloCoalSimulations: A simulator for network multispecies coalescent models, including a new extension for the inheritance of gene flow
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Data from: Distinct patterns of inheritance shape life-history traits in steelhead trout
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Data from: Inheritance of somatic mutations can affect fitness in monkeyflowers
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.