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Dataset results
55 results for “multiplex PCR”
Assessment of a multiplex PCR and Nanopore-based method for portable dengue virus sequencing in Indonesia
<p>Multiplex primer sets for amplification of the complete coding region of Indonesian dengue virus.</p>
Fast Identification of Pathogen in the Setting of Pneumonia Using Multiplex PCR
ClinicalTrials.gov study NCT01858974. IPD Sharing: Not stated. Countries: 1. Publications: 3.
New Multiplex Real-time PCR for Genital Ulcer Disease
ClinicalTrials.gov study NCT01688258. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Detection of Chlamydia Trachomatis, Neisseria Gonorrhoeae and Mycoplasma Genitalium by Real-time Multiplex Polymerase Chain Reaction (PCR) After Pooling Pharyngeal, Anorectal and Urinary Samples
ClinicalTrials.gov study NCT03568695. IPD Sharing: Not stated. Countries: 1. Publications: 5.
Combined Use of a Respiratory Broad Panel Multiplex PCR and Procalcitonin to Reduce Antibiotics Exposure in Hospitalized Sickle-cell Adults With Acute Chest Syndrome.
ClinicalTrials.gov study NCT03919266. IPD Sharing: NO. Countries: 1. Publications: 1.
Simultaneaous Detection of Quantitative Somatic Alterations Using the Qantitative Multiplex Pcr of Short Fluorescent Fragments Method (QMPSF) in Stage II-III Colon Cancer: a Prospective Study
ClinicalTrials.gov study NCT02110329. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Multiplex PCR -Microarray-based Assay in the Detection of Mycobacterium
ClinicalTrials.gov study NCT03794284. IPD Sharing: NO. Countries: 1. Publications: 11.
Effect of the Use of Probiotic S. Boulardii on Acute Viral Inflammatory Diarrhea Diagnosed With Multiplex PCR.
ClinicalTrials.gov study NCT05226052. IPD Sharing: NO. Countries: 1. Publications: 14.
DiagNostic Study of Low-dose CT and multipleX PCR on Antibiotic Treatment and Outcome of Community-Acquired Pneumonia
ClinicalTrials.gov study NCT03360851. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Data from: Disentangling mite predator-prey relationships by multiplex PCR
Open the record for dataset details and reuse information.
Data from: Multiplex preamplification PCR and microsatellite validation allows accurate single nucleotide polymorphism (SNP) genotyping of historical fish scales
Open the record for dataset details and reuse information.
FIGURE 1. A, B in A multiplex PCR method for identification of two common true cutworm species (Lepidoptera: Noctuidae) tested in the central plain of Guilan province, Iran
FIGURE 1. A, B, Maize and green pepper fields damaged by true cutworm.
The Clinical and Pharmacoeconomic Impact of Rapid Diagnostic Test (Multiplex PCR FilmArray) on Antimicrobial Decision Making Compared to Conventional Decision Making Among Critically Ill Patients
ClinicalTrials.gov study NCT05759494. IPD Sharing: UNDECIDED. Countries: 0. Publications: 4.
Data from: Scaling up DNA barcoding - primer sets for simple and cost efficient arthropod systematics by multiplex PCR and Illumina amplicon sequencing
1. The simplicity and cost efficiency of Illumina amplicon sequencing has greatly contributed to the advancement of DNA barcoding and metabarcoding applications. However, current amplicon sequencing based barcoding approaches are usually restricted to short, single-locus fragments, limiting their taxonomic and phylogenetic resolution. 2. Here, we establish a cost efficient and simple multiplex PCR protocol for arthropod systematics by Illumina amplicon sequencing. We introduce primer sets, including several new, generic primers, to reliably amplify nine loci across a wide range of arthropods. Using a diverse collection of arthropod species from 19 orders, we test loci for amplification efficiency and estimate the effect of cross-species amplification bias on taxon recovery from bulk community samples. We then explore the taxonomic and phylogenetic utility of the primer sets, focusing on a dataset of spiders that includes both deep and recent divergences. 3. The set of loci provides good phylogenetic support across a wide taxonomic spectrum, making it a useful addition to COI for resolving lineages within a comparative context. All loci recover sequences for the majority of arthropod taxa in separate PCRs. However, cross-species amplification bias in some primers prevents an exhaustive taxon recovery from bulk community samples. 4. Our protocol makes it possible to generate multilocus datasets for large numbers of arthropod taxa for a fraction of the price and workload of Sanger sequencing. This opens up the possibility for parallel phylogenetic and taxonomic analysis of large collections of arthropods, but also enables rapid exploratory analyses of target lineages. Primers for metabarcoding applications should be carefully evaluated for their performance in bulk community samples and chosen to minimize cross-species amplification bias.
Data from: Multiplex PCR targeting lineage specific SNPs ‐ A highly efficient and simple approach to block out predator sequences in molecular gut content analysis
Background: Food webs form the basis of biological communities, though empirical research has been hindered by difficulties in quantifying interactions. Metabarcoding from predator gut content extractions with universal primers promises to provide simple and rapid insights into food web interactions. However, the highly overabundant predator DNA often completely outcompetes that of the digested prey DNA during PCR, impeding the ability to assess the abundance and diversity of prey items. Methods: Focusing on the issue of overabundance of predator DNA amplified by a commonly used COI primer pair, we use predator lineage-specific SNPs at the 3'-end of PCR primers to selectively block out predators from amplification. While this approach largely prevents predator amplification, it retains high taxonomic versatility for prey lineages. We introduce a novel multilocus assay, targeting four nuclear and mitochondrial rDNA markers and test our approach in a diverse set of spiders from 12 families. We estimate the recovered prey DNA proportions and compare the taxonomic composition of prey communities between markers. Using a feeding experiment, we also explore recovery of prey DNA over time. Results: While commonly used COI primers yield low and very unpredictable amounts of prey DNA, our assay allows for a considerable and consistent prey enrichment across all tested species. The recovered prey's taxonomic composition is comparable between markers and supports results acquired by COI. The new marker set can be amplified in a simple multiplex PCR, considerably reducing the necessary workload. Conclusions: Our multi-locus approach allows the generation of an unprecedented amount of prey data at low cost and effort. Lineage specific PCR is taxonomically versatile and could readily be adapted to any prey-predator interaction, opening up the opportunity for community-wide studies on food web interactions.
Evaluation of a New Multiplex Quantitative PCR Technique for the Diagnosis of Echinococcosis
ClinicalTrials.gov study NCT05824442. IPD Sharing: NO. Countries: 1. Publications: 0.
Ventilator Associated Pneumonia Multiplex PCR for Anti-Infective Regimens
ClinicalTrials.gov study NCT06554327. IPD Sharing: NO. Countries: 1. Publications: 0.
Multiplex Respiratory PCR Results in Diabetic Pneumonia Cases
ClinicalTrials.gov study NCT06459713. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Use of a Respiratory Multiplex PCR and Procalcitonin to Reduce Antibiotics Exposure in Patients With Severe Confirmed COVID-19 Pneumonia
ClinicalTrials.gov study NCT04334850. IPD Sharing: NO. Countries: 1. Publications: 0.
Diagnostic Modalities for Severe Odontogenic Infections Using Rapid, Target Enriched Multiplex PCR (TEM- PCR) by Diatherix
ClinicalTrials.gov study NCT03772769. IPD Sharing: NO. Countries: 1. Publications: 0.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.