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Multispectral absorbance and fluorescence analysis of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water years 2017 and 2018 (1 Oct 2016 - 30 Sep 2018)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding likely to be extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are multispectral absorbance and fluorescence analyses of organic carbon dissolved in samples of well-mixed river thalweg water. Data include excitation-emission matrices, absorbance spectroscopy, as well as absorbance and fluorometric summary indices calculated at specific wavelengths of excitation and emission. Data are from the 2017 and 2018 water years (1 Oct 2016 to 30 Sep 2018). Data were collected on the Upper Clark Fork River (USGS HUC 17010201) at 13 project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA. These data are a correction of a previously published data product (doi:10.6073/pasta/6ba30f4ebb63175a4399c5d0aa6a8698). Inconsistencies between availability of EEMS data, absorbance data, and fluorometric summary metrics have been corrected. P
Multispectral absorbance and fluorescence analysis of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2019 (1 Oct 2018 - 30 Sep 2019)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding likely to be extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are multispectral absorbance and fluorescence analyses of organic carbon dissolved in samples of well-mixed river thalweg water. Data include excitation-emission matrices, absorbance spectroscopy, as well as absorbance and fluorometric summary indices calculated at specific wavelengths of excitation and emission. Data are from the 2019 water year (1 Oct 2018 to 30 Sep 2019). Data were collected on the Upper Clark Fork River (USGS HUC 17010201) at 13 project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Time series of high-frequency profiles of depth, temperature, dissolved oxygen, conductivity, specific conductance, chlorophyll a, turbidity, pH, oxidation-reduction potential, photosynthetically active radiation, colored dissolved organic matter, phycocyanin, phycoerythrin, and descent rate for Beaverdam Reservoir, Carvins Cove Reservoir, Falling Creek Reservoir, Gatewood Reservoir, and Spring Hollow Reservoir in southwestern Virginia, USA 2013-2025
Depth profiles of water biogeochemical properties were collected with SeaBird Electronics (SBE) Conductivity, Temperature, and Depth (CTD) profilers from 2013-2025 at five drinking water reservoirs in southwestern Virginia, USA. The study reservoirs are: Beaverdam Reservoir (Vinton, Virginia), Carvins Cove Reservoir (Roanoke, Virginia), Falling Creek Reservoir (Vinton, Virginia), Gatewood Reservoir (Pulaski, Virginia), and Spring Hollow Reservoir (Salem, Virginia). Beaverdam, Carvins Cove, Falling Creek, and Spring Hollow Reservoirs are owned and operated by the Western Virginia Water Authority as primary or secondary drinking water sources for Roanoke, Virginia, and Gatewood Reservoir is a drinking water source for the town of Pulaski, Virginia. The dataset consists of CTD depth profiles measured at the deepest site of each reservoir adjacent to the dam as well as other upstream reservoir sites. The profiles were collected approximately fortnightly in the spring months, weekly in the summer and early autumn, and monthly in the late autumn and winter. Beaverdam Reservoir, Carvins Cove Reservoir, and Falling Creek Reservoir were sampled every year in the dataset (2013-2025); Spring Hollow Reservoir was only sampled 2013-2017 and 2019; and Gatewood Reservoir was only sampled in 2016. Data availability differs across years due to additional sensors that have been added or replaced over time. From 2013-2016, profiles were taken with a CTD equipped with an SBE 43 Dissolved Oxygen sensor and an ECO FLNTU sensor for turbidity and chlorophyll. From 2017-2025, profiles were taken with a CTD equipped with an SBE 43 Dissolved Oxygen sensor, an ECO FLNTU sensor for turbidity and chlorophyll, a PAR-LOG ICSW sensor for photosynthetically active radiation, and a SBE 27 pH and ORP (oxidation-reduction potential) sensor. In 2022 and 2023, profiles were also taken with an additional CTD equipped with an SBE 43 Dissolved Oxygen sensor; an ECO Triplet Scattering Fluorescence sensor for
Non-Targeted Screening of Organic Compounds in Environmental and Biological Matrices Related to Children's Environmental Exposure in South Florida, 2022-2024
This dataset provides a comprehensive list of chemicals relevant to children’s exposure from both dietary and non-dietary sources, across five environmental and biological matrices: drinking water (n = 206), food (n = 203), urine (n = 183), soil (n = 178), and household dust (n = 164). Samples were collected between May 2022 and June 2024 in Miami-Dade and Broward counties, Florida. A non-targeted screening approach using high-resolution mass spectrometry (HRMS) coupled with liquid chromatography was employed for analysis, with matrix-specific preparation methods: online solid-phase extraction (SPE) for water and urine, QuEChERS for food, and accelerated solvent extraction (ASE) for soil and dust. Analyses were conducted in full-scan mode under both positive and negative electrospray ionization to maximize compound detection coverage. Compound identification was performed using Compound Discoverer software, incorporating spectral and structural databases such as mzCloud, ChemSpider, ClassyFire, and MassList. Annotations were based on exact mass, mass error threshold (<5ppm), predicted molecular formula, retention time alignment, isotopic pattern fit, MS/MS spectral similarity, and match confidence levels derived from integrated spectral libraries and database scoring algorithms. Quality assurance was maintained through the use of quality control (QC) samples across all matrices and analytical batches. The integration of non-targeted analysis, matrix-optimized extraction, and rigorous QA/QC practices makes this dataset a valuable resource for environmental exposomics, chemical risk assessment, and evidence-based public health policy development.
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2021 (1 Oct 2020 - 30 Sep 2021)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are Aurora Total Organic Carbon combustion analyses of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2021 water year (1 Oct 2020 to 30 Sep 2021) from samples collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2023 (1 Oct 2022 - 30 Sep 2023)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are total organic carbon combustion analyses (Shimadzu Scientific Instruments) of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2023 water year (1 Oct 2022 to 30 Sep 2023) from samples collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2024 (1 Oct 2023 - 30 Sep 2024)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are total organic carbon combustion analyses (Shimadzu Scientific Instruments) of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2024 water year (1 Oct 2023 to 30 Sep 2024) from samples collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Soil dissolved organic matter and greenhouse gas fluxes from intact Delmarva Bay wetland soil cores during laboratory simulation of groundwater level rise
Wetlands in low-relief landscapes have dynamic terrestrial-aquatic interfaces as surface water and groundwater levels fluctuate seasonally and these variable water levels influence wetland carbon cycling. Seasonal changes in groundwater levels determine which soils are hydrologically connected to the wetland surface water-groundwater continuum and therefore which soils act as carbon sources. To quantify groundwater-mediated soil dissolved organic matter (DOM) and greenhouse gas (CO2 and CH4) fluxes, we performed a laboratory simulation of groundwater rise on intact soil cores. Soil cores were collected from four Delmarva Bay wetlands located in the low-relief landscape of the Delmarva Peninsula in the Mid-Atlantic United States. At each wetland, two cores (length = 60 cm, diameter = 10.2 cm) were collected: one from within the wetland basin and the second from the transitional zone near the edge of the wetland basin (total number of cores collected = 8). To characterize seasonal hydrologic conditions at each wetland site where intact soil cores were collected (e.g., mean water level, number of saturation events, duration of saturation), we used high frequency water level data collected in wetland center and upland groundwater monitoring wells. In the lab, cores were re-saturated with groundwater over 15 days and after cores were fully re-saturated, core water levels were maintained for an additional 25 days. Water levels in each head tank and soil core were manually recorded on sub-daily timesteps. Rhizon soil porewater samplers were installed at 8, 20, and 40 cm below the soil surface reflecting the expected depths of the O, A, and B soil horizons across the wetland sites. Source groundwater, soil porewater, and exfiltrated surface water samples were collected daily from the soil cores and analyzed for pH, ORP, and DOM concentration (dissolved organic carbon) and DOM composition (absorbance and fluorescence metrics). Discrete measurements of CO2 and CH4 fluxes were
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2022 (1 Oct 2021 - 30 Sep 2022)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are primarily Aurora Total Organic Carbon combustion analyses of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. A few samples from the final campaign in the dataset were analyzed with a Shimadzu instrument using a similar method. Data are from the 2022 water year (1 Oct 2021 to 30 Sep 2022) from samples collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Time series of high-frequency sensor data measuring water temperature, dissolved oxygen, pressure, conductivity, specific conductance, total dissolved solids, chlorophyll a, phycocyanin, fluorescent dissolved organic matter, and turbidity at discrete depths in Falling Creek Reservoir, Virginia, USA in 2018-2025
We monitored water quality in Falling Creek Reservoir (Vinton, Virginia, USA; 37.30325 -79.8373) with high-frequency (10-minute) sensors in 2018-2025. All variables were measured at the deepest site of the reservoir adjacent to the dam. Falling Creek Reservoir is owned and managed by the Western Virginia Water Authority as a primary drinking water source for Roanoke, Virginia. This data product consists of one dataset compiled of depth profiles of water temperature on 1-m intervals from 0.1 to 9 m depth; dissolved oxygen at 5 m and 9 m depth; pressure at 9 m depth; and temperature, dissolved oxygen, conductivity, specific conductance, chlorophyll a, phycocyanin, total dissolved solids, fluorescent dissolved organic matter, turbidity, and pressure at ~1.6 m depth. The dataset is accompanied by a sensor maintenance log and quality assurance/quality control analysis scripts.
Time series of high-frequency sensor data measuring water temperature, dissolved oxygen, conductivity, specific conductance, total dissolved solids, chlorophyll a, phycocyanin, fluorescent dissolved organic matter, and turbidity at discrete depths, and water level in Beaverdam Reservoir, Virginia, USA in 2009-2025
We monitored water level and water quality in Beaverdam Reservoir (Vinton, Virginia, USA; 37.31288, -79.8159) with visual observations and high-frequency (10- to 15-minute resolution) sensors in 2009-2025. All variables were measured at the deepest site of the reservoir adjacent to the dam. Beaverdam Reservoir is owned and managed by the Western Virginia Water Authority as a secondary drinking water source for Roanoke, Virginia. This data package is comprised of three datasets: 1) bvre-waterlevel_2009_2025.csv, 2) bvre-sensorstring_2016_2020.csv, and 3) bvre-waterquality_2020_2025.csv. 1) bvre-waterlevel_2009_2025.csv contains water level observations of the staff gauge at a platform near the reservoir's dam by both the Western Virginia Water Authority and the Virginia Tech Reservoir Group LTREB field crew. This dataset spans 2009 to 2025, with data collection still ongoing. 2) bvre-sensorstring_2016_2020.csv consists of a water temperature profile at ~1-meter intervals from the surface of the reservoir to 10.5 m below the water, complemented by intermittent data collected by a dissolved oxygen logger deployed at 5 m or 10 m. A sonde measuring water temperature, conductivity, specific conductance, chlorophyll a, phycocyanin, total dissolved solids, dissolved oxygen, fluorescent dissolved organic matter, and turbidity was additionally deployed at ~1.5 m depth. This dataset spans 2016 to 2020, with no additional data collection beyond the last observation. The third dataset is bvre-waterquality_2020_2025.csv, with data collection still ongoing and an accompanying maintenance log. This dataset contains: a) a temperature string with 13 temperature sensors deployed ~1 m apart from the surface to 0.5 m above the sediments of the reservoir; b) two dissolved oxygen sensors, one in the middle of the string and one sensor above the sediments; and c) a pressure sensor just above the sediments. The same sonde from the first 2016-2020 dataset is also included in this 2020-2025 d
Long term response of arctic tussock tundra to thermal erosion features: A modeling analysis. Tussock tundra regrowth after a thermal erosion event: Simulation A - increased Phase II soil organic matter
The Multiple Element Limitation (MEL) model is used to simulate the recovery of Alaskan arctic tussock tundra to thermal erosion features (TEFs) caused by permafrost thaw and mass wasting. TEFs could be significant to regional carbon (C) and nutrient budgets because permafrost soils contain large stocks of soil organic matter (SOM) and TEFs are expected to become more frequent as climate warms. These simulations deal only with recovery following TEF stabilization and do not address initial losses of C and nutrients during TEF formation. To capture the variability among and within TEFs, we simulate a range of post-stabilization conditions by varying the initial size of SOM pools and nutrient supply rates. This file contains the results for 100 years of tussock tundra recovery after a thermal erosion event. This simulation is of TEF recovery with increased Phase II soil organic matter compared to the base simulation. Data is presented for day 250 of each year.
Long term response of arctic tussock tundra to thermal erosion features: A modeling analysis. Tussock tundra regrowth after a thermal erosion event: Simulation C - increased Phase I and Phase II soil organic matter
The Multiple Element Limitation (MEL) model is used to simulate the recovery of Alaskan arctic tussock tundra to thermal erosion features (TEFs) caused by permafrost thaw and mass wasting. TEFs could be significant to regional carbon (C) and nutrient budgets because permafrost soils contain large stocks of soil organic matter (SOM) and TEFs are expected to become more frequent as climate warms. These simulations deal only with recovery following TEF stabilization and do not address initial losses of C and nutrients during TEF formation. To capture the variability among and within TEFs, we simulate a range of post-stabilization conditions by varying the initial size of SOM pools and nutrient supply rates. This file contains the results for 100 years of tussock tundra recovery after a thermal erosion event. This simulation is of TEF recovery with increased Phase I and Phase II soil organic matter compared to the base simulation. Data is presented for day 250 of each year.
Long term response of arctic tussock tundra to thermal erosion features: A modeling analysis. Tussock tundra regrowth after a thermal erosion event: Simulation D - reduced Phase I and Phase II soil organic matter
The Multiple Element Limitation (MEL) model is used to simulate the recovery of Alaskan arctic tussock tundra to thermal erosion features (TEFs) caused by permafrost thaw and mass wasting. TEFs could be significant to regional carbon (C) and nutrient budgets because permafrost soils contain large stocks of soil organic matter (SOM) and TEFs are expected to become more frequent as climate warms. These simulations deal only with recovery following TEF stabilization and do not address initial losses of C and nutrients during TEF formation. To capture the variability among and within TEFs, we simulate a range of post-stabilization conditions by varying the initial size of SOM pools and nutrient supply rates. This file contains the results for 100 years of tussock tundra recovery after a thermal erosion event. This simulation is of TEF recovery with reduced Phase I and Phase II soil organic matter compared to the base simulation. Data is presented for day 250 of each year. .
Long term response of arctic tussock tundra to thermal erosion features: A modeling analysis. Tussock tundra regrowth after a thermal erosion event: Simulation B - increased Phase I soil organic matter
The Multiple Element Limitation (MEL) model is used to simulate the recovery of Alaskan arctic tussock tundra to thermal erosion features (TEFs) caused by permafrost thaw and mass wasting. TEFs could be significant to regional carbon (C) and nutrient budgets because permafrost soils contain large stocks of soil organic matter (SOM) and TEFs are expected to become more frequent as climate warms. These simulations deal only with recovery following TEF stabilization and do not address initial losses of C and nutrients during TEF formation. To capture the variability among and within TEFs, we simulate a range of post-stabilization conditions by varying the initial size of SOM pools and nutrient supply rates. This file contains the results for 100 years of tussock tundra recovery after a thermal erosion event. This simulation is of TEF recovery with increased Phase I soil organic matter compared to the base simulation. Data is presented for day 250 of each year.
Long term response of arctic tussock tundra to thermal erosion features: A modeling analysis. Tussock tundra regrowth after a thermal erosion event: Simulation E - reduced Phase I soil organic matter
The Multiple Element Limitation (MEL) model is used to simulate the recovery of Alaskan arctic tussock tundra to thermal erosion features (TEFs) caused by permafrost thaw and mass wasting. TEFs could be significant to regional carbon (C) and nutrient budgets because permafrost soils contain large stocks of soil organic matter (SOM) and TEFs are expected to become more frequent as climate warms. These simulations deal only with recovery following TEF stabilization and do not address initial losses of C and nutrients during TEF formation. To capture the variability among and within TEFs, we simulate a range of post-stabilization conditions by varying the initial size of SOM pools and nutrient supply rates. This file contains the results for 100 years of tussock tundra recovery after a thermal erosion event. This simulation is of TEF recovery with decreasing Phase I soil organic matter compared to the base simulation. Data is presented for day 250 of each year.
Composition and biodegradability of dissolved organic matter leached from eroding coastal soils and permafrost in seawater, from Drew Point, Alaska
Eroding permafrost coastlines export significant quantities of organic carbon (OC) to the marine environment, similar in magnitude to riverine particulate OC fluxes to the Arctic Ocean. Moreover, erosion rates are predicted to increase due to warming temperatures, declines in sea ice, and increasing waves. While erosion primarily mobilizes organic matter in the particulate form, this material can be leached to dissolved organic matter (DOM). This DOM may be incorporated by microbial communities and fuel marine food webs or decomposed to form greenhouse gases like carbon dioxide and methane. Many studies show that permafrost-derived organic matter can be rapidly decomposed in soils and freshwater, but few studies examine the fate of permafrost organic matter in seawater. To address this knowledge gap, we designed a laboratory experiment to leach coastal soils and permafrost in seawater and examine the composition and biodegradability of leached DOM. Coastal soil/sediment was cored near Drew Point, Alaska in 2019, representing three horizons found within rapidly eroding permafrost bluffs: seasonally thawed active layer soils, Holocene terrestrial soils and/or lacustrine sediments, and late-Pleistocene relict marine sediments. To measure dissolved organic carbon (DOC) leaching yields, we placed soil/sediments in Beaufort Sea seawater for 24 hours before filtering to remove particulates. To measure biodegradable dissolved organic carbon (BDOC), we conducted an aerobic incubation experiment following the methods suggested by Vonk et al. (2015). Briefly, leachates were incubated at approximately room temperature for 26 and 90 days to measure DOC loss due to remineralization and/or incorporation into microbial biomass. Additionally, we used chromophoric dissolved organic matter (CDOM) measurements and ultra-high resolution mass spectrometry (FT-ICR MS) to examine the initial leachate DOM composition. References: Vonk, J. E., Tank, S. E., Mann, P. J., Spencer, R. G. M., Tre
Stream Suspended Sediment and Particulate Organic Matter at Harvard Forest 2009-2010
In addition to conveying water and nutrients and providing habitat to a variety of ecosystems, streams transport downstream mineral sediment and other particulate matter washed in from hillslopes and eroded from its channel and banks. At high levels, suspended sediment can be a devastating pollutant for aquatic organisms. The amount of suspended material in a stream varies tremendously with discharge; typically, suspended sediment increases with discharge, as stormwater runoff and overland flow carry particles from the hillslopes into the channel. Suspended sediment can also be a function of land use and vegetation, both of which affect the infiltration capacity of the landscape; more infiltration generally means less surface runoff and thus less sediment. Forested watersheds such as the Bigelow Brook watershed will typically have less suspended sediment than similar watersheds in urban environments. By analyzing how suspended sediment varies with discharge, I will be able to compare the relative effectiveness of overland flow of stormwater in washing materials into the streams. It is also possible that tree loss due to the wooly adelgid, ice storms, or fire in the watershed may increase the amount of sediment to Bigelow Brook, as a loss in tree canopy may result in more soil erosion due to rain splash and more water overall reaching the stream. For this reason, I hope to continue monitoring sediment in Bigelow Brook for an extended period of time to record any significant changes due to changing vegetation. Furthermore, by determining how much of the suspended sediment consists of particulate organic matter (using standard LOI techniques), I will be able to estimate the net carbon export from the two watersheds via that pathway. Preliminary, back-of-the-envelope calculations suggest that as much as 3-5% of the total annual carbon export leaves the Harvard Forest watershed via stream-transported particulate organic matter. To this end, I propose to measure suspended
Molecular composition of dissolved organic matter in NTL-LTER lakes detected by Fourier-transform ion cyclotron resonance mass spectrometry
The composition of dissolved organic matter (DOM) varies widely in the environment due to distinct sources of the material and subsequent processing. DOM composition drives its reactivity in terms of many processes including photochemical reactions, microbial metabolism, and carbon cycling within water bodies. This study uses ultra-high resolution mass spectrometry via a Fourier-transform ion cyclotron resonance mass spectrometer (FT-ICR MS) to evaluate DOM composition at the molecular level to determine differences in DOM composition among the NTL-LTER lakes. Whole water samples were collected from the surface of each lake near the shore on August 18th and 19th in 2016 in. Ultraviolet-visible spectra were recorded as light absorbance can also give information about DOM composition. Additionally, concentrations of anions, cations, and pH were measured waters because these can all alter DOM reactivity in the environment. Both water chemistry and DOM composition vary widely among the lakes with the bogs displaying the most terrestrial-like signature in DOM and the oligotrophic lakes show more microbial-like or environmentally processed DOM.
Molecular composition of dissolved organic matter from Lake Mendota from June – November 2017, analyzed by Fourier-transform ion cyclotron resonance mass spectrometry
Dissolved organic matter (DOM) is a complex mixture of organic compounds found in all natural waters. Its composition affects its reactivity towards numerous processes. Its composition is a function of both its source (e.g., allochthonous or autochthonous) as well as the extent of environmental processing it has undergone (e.g., chemical or biological degradation). Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) allows for the characterization of dissolved organic matter at the molecular level. The water sample was collected near the NTL-LTER research buoy on Lake Mendota. Formula assignments were made to raw mass to charge ratios detected in the mass spectrum using a custom processing script and resulting in a list of chemical formulas making up the DOM sample.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.