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Colored dissolved organic matter (CDOM) absorbance from lagoon, ocean, and river sites along the Alaska Beaufort Sea coast, 2021-ongoing
Multiple water types (river, lagoon, ocean) from the North Slope of Alaska and nearshore Beaufort Sea are sampled seasonally by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Water samples from multiple depths are collected during full ice cover (April), ice break-up (mid-June to early July), and open water (late July and August) periods, filtered, and analyzed for light absorption spectra within 24 hours of collection. Wavelength-specific light absorption coefficients are reported between 250 and 600 nanometers. The data is organized in "long" or "tidy" format, with columns of station, date, wavelength, and absorption. Please see included MakeColumnsAsWavelengths.R, MakeColumnsAsDates.R, and ReshapeDataInExcel.txt for some common ways to reorganize the table for further CDOM analysis. In 2022, data from 2019 were removed due to quality issues (please see revision 1 of this dataset for 2019 CDOM values). For users who may have used 2019 data from this dataset, there are additional files to inform decisions going forward. "BLE_LTER_CDOM_2019_sample_flags.csv" lists which 2019 samples are entirely unreliable, versus usable with caution. "BLE_LTER_CDOM_2021_blank_mean_sd_absorptions.csv" lists mean and standard deviations at each wavelength from all blanks taken in 2021; this is meant to give info on the instruments used and will not be updated further. Please see the methods section for more information on 2019 data.
Soil salinity and organic content at GCE-LTER vegetation monitoring plots in October 2020
Soil samples were collected in conjunction with Fall 2020 plant monitoring at half of the permanent vegetation monitoring plots in the creekbank and midmarsh zones at 10 GCE study sites. Pore-water salinity was determined by analysis of supernatant salinity in dried soil samples hydrated with a measured volume of deionized water. Organic content was measured gravimetrically by comparing ash-free dry weight and total weight of soil samples.
Georgia Salt Marsh: Soil Organic Carbon, Nitrogen, Bulk Density, Moisture, and Texture
As part of project predicting soil carbon at depth from that found at the surface using remote sensing, 28 soil cores were taken from six salt marshes along the Georgia coastline. Cores were taken as deep as possible (25 – 165 cm) and sectioned into 5 cm depths. Soils were analyzed for organic carbon (SOC), total nitrogen (N), bulk density (BD), and particle size (by horizon). Stable carbon isotopes were obtained in three marshes on Sapelo Island; a subset was also analyzed for radiocarbon.
Harmonized Soil Organic Carbon and Phosphorus Data for the Contiguous United States
Soil organic carbon (SOC) and soil phosphorus can strongly influence adjacent water quality by introducing nutrients into aquatic ecosystems and also altering the light environment of those ecosystems. However, national-scale data are uncommon, and even when available, they are usually not aggregated in a manner that is expeditiously merged with basin-level data. To facilitate national-scale analyses of soil data with co-located water quality data, we present aggregated SOC and soil phosphorus data for the Contiguous United States. Data are aggregated using the HydroBASINS basin shapefiles. HYBAS_ID is retained to enable merging with HydroBASINS parent datasets.
Carbon and nitrogen content and stable isotope compositions from particulate organic matter samples from lagoon, river, and open ocean sites along the Alaska Beaufort Sea coast, 2018-ongoing
Multiple water types (river, lagoon, ocean) from the North Slope of Alaska and nearshore Beaufort Sea are sampled seasonally by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Water samples are collected during full ice cover (April), ice break-up (mid-June to early July), and open water (late July and August) periods, and analyzed for particulate organic carbon (POC) and particulate organic nitrogen (PON) content and stable isotopic composition.
Carbon and nitrogen content and stable isotope composition from sediment organic matter from lagoon sites along the Alaska Beaufort Sea coast, 2018-ongoing
Multiple sediment samples from lagoons along the nearshore Beaufort Sea are sampled seasonally by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Sediment samples are collected during full ice cover (April), ice break-up (mid-June to early July), and open water (late July and August) periods, and analyzed for carbon and nitrogen content and stable isotopic composition.
Dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) from river, lagoon, and open ocean sites along the Alaska Beaufort Sea coast, 2018-ongoing
Multiple water types (river, lagoon, ocean) from the North Slope of Alaska and nearshore Beaufort Sea are sampled seasonally by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Water samples are collected during full ice cover (April), ice break-up (mid-June to early July), and open water (late July and August) periods and analyzed for dissolved organic carbon and total dissolved nitrogen content.
Decomposition of soil and permafrost organic matter eroding into the Beaufort Sea near Drew Point, Alaska
Arctic coastal erosion mobilizes large quantities of permafrost organic matter to the Arctic Ocean, where it may be decomposed, releasing carbon dioxide. To quantify the biodegradability of this eroding material, we designed an aerobic bottle incubation experiment to measure CO2 production from coastal soils/sediments submerged in seawater. Seasonally thawed active layer soils and permafrost were sampled near Drew Point along the Alaska Beaufort Sea coast. Cores were taken from three surface geomorphic classifications common in this area: primary surface that has not been reworked by thaw-lake cycles, a young drained lake basin, and an ancient drained lake basin. Core subsamples were chosen to represent three distinct horizons present in eroding bluffs at Drew Point: seasonally thawed active layer soils near the tundra surface, Holocene-age terrestrial soils and/or lake sediments, and late-Pleistocene age relict marine sediments. Soil/sediment subsamples were mixed with Beaufort Sea surface water and incubated in triplicate at 4C and 16C for 40 days. In addition, a subset of soil/sediment samples were incubated with and without seawater at 16C for 40 days. The data reported here summarizes the results of the incubation experiment for each soil sample: cumulative CO2-C production over 40 days normalized to dry weight and to organic carbon content (OC), average rate of CO2 production normalized to dry weight and to TOC, and the percent of OC remineralized over 40 days.
Composition and biodegradability of dissolved organic matter in supra-permafrost groundwater and surface waters near Simpson Lagoon, Alaska
Supra-permafrost groundwater (SPGW) is an important source of terrestrial dissolved organic matter (DOM) to the Arctic Ocean, yet few studies have investigated the quality or characteristics of this DOM. We sampled fresh SPGW, run-off, and rivers near Simpson Lagoon, Alaska during spring ice break-up (mid-June), summer open water (late July), and fall freeze-up (late September - early October). We measured dissolved organic carbon (DOC) concentrations in these samples and analyzed the composition of DOM using high-resolution mass spectrometry (Fourier transform ion cyclotron resonance mass spectrometry; FT-ICR MS). To measure biodegradable dissolved organic carbon (BDOC), we conducted an aerobic incubation experiment following the methods suggested by Vonk et al. (2015). Briefly, water samples were incubated at 20C for 28 days to measure DOC loss due to remineralization by in-situ microbial communities.
Total dissolved nitrogen (TDN), dissolved organic carbon (DOC), radiocarbon (14C-DOC), and stable carbon (13C-DOC) of surface waters from the Canning River watershed, 2019 and 2021
Sites along the Canning River mainstem and contributing streams near the Kavik River Camp, Alaska, were visited to track changes in stream and river total dissolved nitrogen (TDN) concentration, dissolved organic carbon (DOC) concentration, and the stable carbon (13C) and radiocarbon (14C) isotopic composition of DOC across transitions between the Brooks Range, Brooks foothills, and Arctic Coastal Plain. The dataset also includes water samples collected from lakes, springs, groundwater, and streams and rivers outside the Canning River watershed. Water samples were collected in late April and early August 2019 and in late July and early August 2021. Data include measurements of individual samples for TDN (milligrams nitrogen per liter), DOC (milligrams carbon per liter), carbon-13 of DOC (reported as delta-13C, per mil), carbon-14 of DOC (reported as fraction modern), and analytical error in the fraction modern values. Additional water chemistry data for these samples can be found in Koch et al. (2024). References: Koch, J. C., Connolly, C. T., Repasch, M., Best, H. R., Couvillion, C. S., Hunt, A. (2024). [Dataset] Hydrochemistry and age date tracers from springs, streams, and rivers in the Arctic National Wildlife Refuge, 2019-2022, U.S. Geological Survey data release, https://doi.org/10.5066/P95CXJIT.
Ramped Pyrolysis Oxidation (RPO) coupled radiocarbon (14C-DOC) and stable carbon (13C-DOC), high-resolution molecular composition (FT-ICR MS), and biodegradable dissolved organic carbon (BDOC) of groundwater, river water, and lagoon water in northeast Alaska, 2017
Supra-permafrost groundwater (SPGW), river water, and lagoon water were sampled near Kaktovik, AK to assess the reactivity and origin of dissolved organic matter (DOM) across interconnected hydrologic systems during late summer. Water samples were collected on August 17th 2017 from SPGW along the beach of Jago Lagoon (Jago GW), surface water from the Jago River’s main channel above tidal influence (Jago R), and from the water column of Kaktovik Lagoon at 2–3 m depth (KA LW). Measurements were made from grab samples for river and lagoon water, and from a composite sample for SPGW gathered from 10 individual shoreline locations. Data include dissolved organic carbon concentration (DOC, mg C L-1), Ramped Pyrolysis Oxidation (RPO) derived fraction compositions of 13C-DOC (δ13C ‰), 14C-DOC (in fraction modern), and method/instrumental error in the 14C and 13C results, and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) molecular composition and summarized compound classes. Biodegradable DOC (BDOC) bottle experiments were performed using all three sample types, where DOC concentration was subsequently measured at 2, 7, 14, and 28 days. FT-ICR MS composition was subsequently measured at the 28-day timepoint to track changes in molecular formulae and compound class relative abundance following biodegradation. Data from RPO serial thermal oxidation include temperature and normalized CO2 profiles for each background sample. Thermal-oxidation profiles of CO2 were transformed into non-parametric activation energy (E) distributions using an inverse model. Model output includes C mass of oxidized CO2 (µg C), Tmax (K), Emax (kJ mol-1), Emean (kJ mol-1), Estd (kJ mol-1), and p(0,E)max of user-defined sample fractions. FT-ICR MS results include a summary table of the relative abundance of compound classes (e.g., unsaturated phenolic, polyphenolic, aliphatic, condensed aromatics, peptide-like) and elemental groupings (e.g., CHO-type, CHON-type, CHOS-type, CHON
Long-term soil salinity and organic content from the Georgia Coastal Ecosystems LTER Project on Sapelo Island, Georgia
Soil samples were collected in conjunction with annual plant monitoring at half of the permanent vegetation monitoring plots in the creekbank and midmarsh zones at 10 GCE study sites beginning in 2009. Pore-water salinity was determined by analysis of supernatant salinity in dried soil samples hydrated with a measured volume of deionized water. Organic content was measured gravimetrically by comparing ash-free dry weight and total weight of soil samples.
Comparing vertical accretion, organic carbon (C) sequestration, and nitrogen burial between a natural, never diked tidal salt marsh and a hydrologically restored tidal salt marsh on Sapelo Island, Georgia.
Restoration of tidal marshes throughout the 20th century have attempted to bring back important functions of natural tidal systems. In this study, vertical accretion, organic carbon (C) sequestration, and nitrogen burial were compared between a natural, never diked tidal salt marsh and a hydrologically restored tidal salt marsh on Sapelo Island, Georgia to examine the impacts of restoration years later. On Sapelo Island there are two marshes near the University of Georgia Marine Institute, one of which is a natural marsh, and one of which is a restored marsh. The restored marsh had been diked in 1948, and the dike was breached, allowing for the marsh to be restored, in 1956. Soil cores were collected from both marshes, and the sediments were analysed for Nitrogen and Carbon concentrations and bulk density. This analysis was used to determine accretion rates for the two marshes as well as changes in the restored marsh since the dike was breached. Nitrogen burial, carbon sequestration, and soil accretion in the restored marsh as compared to the natural marsh were the focus of this study.
Organic and Inorganic Nitrogen Uptake by Sarracenia Purpurea at Harvard Forest and Fort Albany ON 2007
The nitrogen-limited carnivorous pitcher plant Sarracenia purpurea and its associated detritus-based food web is a model system for studying plant nutrient dynamics. We tested if S. purpurea can directly take up intact amino acids and compared uptake of organic and inorganic forms of nitrogen (N) across a gradient of N deposition. At sites in Canada and the United States, individual pitchers with complete or incomplete food webs were fed U-13C-15N-glycine, U-13C-15N-phenylalanine and 15NH415NO3 individually and in mixture. Plants took up intact amino acids. Acquisition of each N form provided in isolation exceeded uptake of the same form in mixture. At the high deposition site, uptake of 15N from amino acids was higher than uptake of 15N from inorganic nitrogen. At the low deposition site, uptake of 15N from all three forms of N were similar. Completeness of the associated food web had no effect on 15N uptake. By taking up intact amino acids, Sarracenia purpurea can short-circuit the inorganic N cycle, thus minimizing potential bottlenecks in N availability that result from the plant’s reliance for nitrogen mineralization on a seasonally reconstructed food web operating on infrequent and irregular prey capture.
Correction Factors for Dissolved Organic Carbon Extracted from Soil in New England 2012-2013
Oxidizable dissolved organic carbon (DOC) is regularly measured in environmental samples using a colorimetric method with Mn(III)-pyrophosphate as the oxidizing agent. It is simpler to use and has a much higher throughput than the commonly used dichromate oxidation and combustion methods. Here, we demonstrate that the method often leads to an underestimation or overestimation of the concentration of common organic compounds in solutions. To our knowledge, no published study has taken this fact into account when analyzing DOC data. Hence, we compared Mn(III)-pyrophosphate-based results with measurements performed with a total organic carbon combustion analyzer for samples of organic and mineral soil horizons of two temperate deciduous forests (Harvard Forest, Hubbard Brook), of organic soil horizon of a primary growth hemlock stand (Harvard Forest), and of a peatland (Caribou Bog) located in New England, USA. The Mn(III)-pyrophosphate method consistently underestimated DOC concentration in soil extracts. We present correction factors for the different types of soil studied. By employing correction factors, we find the method can be an inexpensive, accurate, and high throughput tool to measure DOC in environmental samples.
Mercury in soil, vegetation, and organisms across Niwot Ridge, Saddle Catchment, and Green Lakes Valley, 2020 - 2023.
This dataset includes soil, vegetation, water, atmospheric deposition, litterfall, incubation, and organism data from the Niwot Ridge, Saddle Catchment, and Green Lakes Valley collected during 2020 and 2021 to investigate the storage, transformation, and mobilization of mercury in the Colorado Rocky Mountains. During Summer 2020, we collected soil cores (10cm x 3cm) across vegetation plant functional groups in wet meadows, moist meadows, dry meadows, krummholz, subalpine forest, shrub areas, as well as at the inlet and outlet of the Green Lakes in Green Lakes Valley. At each of these sites, we collected leaves from forbs, graminoids, and shrubs, as well as litter (and moss if present). For organisms, we sampled pika hairs from nine different pika trapped on the West Knoll, in addition to caddisfly pupae found in wet meadows in the Saddle Catchment. We analyzed hairs from weasel specimens at the CU Boulder Natural History Museum that were trapped either on, or near, Niwot Ridge. Finally, we analyzed dust samples collected by Dr. Ruth Heindel in 2018 and 2019 on Niwot Ridge. We analyzed soil samples for organic matter; pH; water content; percent carbon, nitrogen, and sulfur; stable carbon, nitrogen, and sulfur isotopes; total mercury; and methylmercury. We analyzed vegetation samples for percent carbon, nitrogen, and sulfur; stable carbon, nitrogen, and sulfur isotopes; total mercury; and methylmercury. We analyzed organism and dust samples for total mercury and methylmercury. During Spring 2021, we collected composite snow cores from 4 sites in the Saddle region and 3 sites in the subalpine forest. We measured snow depth and density to calculate snow water equivalent and then analyzed these samples for sulfate, nitrate, chloride, dissolved organic carbon, dissolved organic nitrogen, total mercury, and methylmercury concentrations. During Summer 2021, we collected soil cores (10cm x 3cm) every other week from June through September from a solifluction lobe, alpine wet
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water years 2017 and 2018 (1 Oct 2016 - 30 Sep 2018)
These data were collected by the University of Montana and Montana State University to support the Upper Clark Fork River restoration monitoring project supported by the US NSF Long Term Research in Environmental Biology (LTREB) program. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are Aurora Total Organic Carbon combustion analyses of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2017 and 2018 water year (1 Oct 2016 to 30 Sep 2018). Data were collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Soil Organic Matter Mechanisms of Stabilization (SOMMOS) - enhanced soil characterization data from 40 National Ecological Observatory Network (NEON) sites
Soil organic matter (SOM) is a critical linkage among many ecosystem services that sustain our society and life on Earth. It is the primary energy source for microbes and the principal storehouse of water necessary for plant growth. SOM also stores nutrients for plants and sorbs pollutants that otherwise could contaminate food and water supplies. Soils also help regulate climate by storing carbon that would otherwise be released to the atmosphere and contribute to climate change. The SOMMOS project investigated processes in the soil that protect SOM from being decomposed by microbes, processes that increase its sensitivity to environmental changes, and how changes in climate and land management influence the amount and stability of SOM. The project, which was a collaboration between scientists from the National Ecological Observatory Network (NEON), University of Colorado, University of Michigan, Oregon State University, Virginia Polytechnic Institute and State University, and the USDA-Forest Service, took advantage of soil samples collected across NEON, a major NSF investment in environmental monitoring that covers the entire United States. This continental-scale soil sample set was analyzed for a wide array of physical and chemical properties, well beyond those typically measured on such a large-scale sample set, including radiocarbon, extractable metals, organic matter chemistry by pyrolysis-GCMS, liquid extract fluorescence spectroscopy, and more. In addition to this dataset, archived samples are available from the project for sharing with interested researchers.
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2020 (1 Oct 2019 - 30 Sep 2020)
These data were collected to support monitoring of the Upper Clark Fork River restoration, and data collection was funded by the US NSF Long Term Research in Environmental Biology (LTREB) program and the US NSF EPSCoR funded Montana Consortium for Research on Environmental Water Systems. The LTREB monitoring project consists of monthly or bi-weekly water quality monitoring across a 200-km restoration gradient contaminated by historic mining practices to monitor inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and heavy metal contamination. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are Aurora Total Organic Carbon combustion analyses of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2020 water year (1 Oct 2019 to 30 Sep 2020). Data were collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Time series of high-frequency sensor data measuring water temperature, dissolved oxygen, conductivity, specific conductance, total dissolved solids, chlorophyll a, phycocyanin, turbidity, and fluorescent dissolved organic matter at discrete depths in Carvins Cove Reservoir, Virginia, USA in 2020-2025
We monitored water quality in Carvins Cove Reservoir (Roanoke, Virginia, USA; 37.3697 -79.958) with high-frequency (10-minute) sensors in 2020-2025. Carvins Cove Reservoir is owned and managed by the Western Virginia Water Authority as a primary drinking water source. This data package consists of datasets from two separate deployments. First, from July 2020 - August 2021, depth profiles of water temperature were measured on 1-meter intervals using HOBO temperature pendant loggers deployed from 0.1 m below the surface of the reservoir to 10 m depth, and also at 15 and 20 m depth. Additionally, water temperature was measured in the Sawmill Branch inflow at 0.5 m depth using HOBO temperature pendant loggers. Second, from 9 April 2021 - 31 December 2025, depth profiles of water temperature were measured on 1-meter intervals from 0.1 m below the surface of the reservoir to 11 m depth and additionally at 15 and 19 m. A YSI EXO2 sonde measured water temperature, conductivity, specific conductance, chlorophyll a, phycocyanin, total dissolved solids, dissolved oxygen, and fluorescent dissolved organic matter at ~1.5 m depth. A YSI EXO3 sonde measured water temperature, conductivity, specific conductance, total dissolved solids, dissolved oxygen, and fluorescent dissolved organic matter at ~9 m depth, which corresponds to the depth of a water outtake valve. The thermistors, EXO3 sonde, and pressure sensor were deployed at stationary, fixed elevations (referred to as positions) deployed off of the dam near the water outtake valves. Due to variable water levels in the reservoir, the depths of these sensors varied over time. In contrast, the EXO2 was deployed on a buoy from 2021-2022 and remained at 1.5 m depth as the water level fluctuated. However, in 2023, the buoy disappeared in a storm, and after that the EXO2 was deployed at a stationary elevation as the water level fluctuated around the sensor. The EXO2 was redeployed on the buoy in 2024. The monitoring site's maximum de
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.