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32 results for “origami”
Fluorophore photostability and saturation in the hotspot of DNA origami nanoantennas
<p>Fluorescent dyes used for single-molecule spectroscopy can undergo millions of excitation-emission<br> cycles before photobleaching. Due to the upconcentration of light in a plasmonic hotspot, the<br> conditions for fluorescent dyes are even more demanding inDNAorigami nanoantennas. Here, we<br> briefly review the current state of fluorophore stabilization for single-molecule imaging and reveal<br> additional factors relevant in the context of plasmonic fluorescence enhancement. Weshow that<br> despite the improved photostability of single-molecule fluorophores byDNAorigami nanoantennas,<br> their performance in the intense electric fields in plasmonic hotspots is still limited by the underlying<br> photophysical processes, such as formation of dim states and photoisomerization. These photophysical<br> processes limit the photon count rates, increase heterogeneity and aggravate quantification of<br> fluorescence enhancement factors. These factors also reduce the time resolution that can be achieved<br> in biophysical single-molecule experiments. Finally, we show how the photophysics of aDNAhairpin<br> assay with a fluorophore-quencher pair can be influenced by plasmonicDNAorigami nanoantennas<br> leading to implications for their use in fluorescence-based diagnostic assays. Especially, we show that<br> such assays can produce false positive results by premature photobleaching of the dark quencher. Here we demonstrate the raw data on which our findings based on.</p>
Day 168: Origami Tiger Mask
3D scan of an origami tiger mask designed by your truly for Chinese New Year. I'll be teaching how to fold this design at Harvard GSAS next week. This one measures 1.6 feet x 1.8 feet. (40 Images, Photo Mode) Created with Polycam Source: Objaverse 1.0 / Sketchfab
Origami-inspired modular electro-ribbon actuator for multi-DoF motion
<p>This upload contains the dataset for the paper: Origami-inspired modular electro-ribbon actuator for multi-DoF motion. The description of the data can be found in the ReadMe file in the folder.</p>
"Robust Folding of Elastic Origami" Data Supplement
<p>Data sets for "Robust Folding of Elastic Origami", meant to be used with Mathematica notebooks provided at <a href="https://github.com/meleetrimble/robust-folding-paper-support">https://github.com/meleetrimble/robust-folding-paper-support</a>.</p>
Data for "Estimation of microtubule-generated forces using a DNA origami nanospring"
<p>Data in support of the publication "Estimation of microtubule-generated forces using a DNA origami nanospring"</p>
Oral Anti-coagulants in Fragile Patients With Percutaneous Endoscopic Gastrostomy and Atrial Fibrillation (ORIGAMI) Pilot Study
ClinicalTrials.gov study NCT04271293. IPD Sharing: UNDECIDED. Countries: 1. Publications: 6.
Using DNA origami nanorulers as traceable distance measurement standards and nanoscopic benchmark structures
<p>In recent years, DNA origami nanorulers for superresolution (SR) fluorescence microscopy have been developed from fundamental proof-of-principle experiments to commercially available test structures. The self-assembled nanostructures allow placing a defined number of fluorescent dye molecules in defined geometries in the nanometer range. Besides the unprecedented control over matter on the nanoscale, robust DNA origami nanorulers are reproducibly obtained in high yields. The distances between their fluorescent marks can be easily analysed yielding intermark distance histograms from many identical structures. Thus, DNA origami nanorulers have become excellent reference and training structures for superresolution microscopy. In this work, we go one step further and develop a calibration process for the measured distances between the fluorescent marks on DNA origami nanorulers. The superresolution technique DNA-PAINT is used to achieve nanometrological traceability of nanoruler distances following the guide to the expression of uncertainty in measurement (GUM). We further show two examples how these nanorulers are used to evaluate the performance of TIRF microscopes that are capable of single-molecule localization microscopy (SMLM).</p> <p>Here we show the raw data the publication is based on.</p>
Supplementary Material: Effects of Handedness and Viewpoint on the Imitation of Origami-Making
<p>Video Supplementary Material: Effects of Handedness and Viewpoint on the Imitation of Origami-Making</p>
The Effect of Origami Activities on Comfort Level and Quality of Life
ClinicalTrials.gov study NCT07296757. IPD Sharing: Not stated. Countries: 1. Publications: 0.
3D model of Origami Crane
3D model of Origami Crane from Wales-Japan Origami Project Source: Objaverse 1.0 / Sketchfab
Fine tuning of CpG spatial distribution with DNA origami for improved cancer vaccination
GEO Series GSE251850. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
Interchromophoric Interactions Determine the Maximum Brightness Density in DNA Origami Structures
<p>An ideal point light source is as small and as bright as possible. For fluorescent point light sources, homogeneity of the light sources is important as well as that the fluorescent units inside the light source maintain their photophysical properties, which is compromised by dye aggregation. Here we propose DNA origami as a rigid scaffold to arrange dye molecules in a dense pixel array with high control of stoichiometry and dye–dye interactions. In order to find the highest labeling density in a DNA origami structure without influencing dye photophysics, we alter the distance of two ATTO647N dyes in single base pair steps and probe the dye–dye interactions on the single-molecule level. For small distances strong quenching in terms of intensity and fluorescence lifetime is observed. With increasing distance, we observe reduced quenching and molecular dynamics. However, energy transfer processes in the weak coupling regime still have a significant impact and can lead to quenching by singlet-dark-state-annihilation. Our study fills a gap of studying the interactions of dyes relevant for superresolution microscopy with dense labeling and for single-molecule biophysics. Incorporating these findings in a 3D DNA origami object will pave the way to bright and homogeneous DNA origami nanobeads.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.