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33 results for “primer evaluation”
Supplementary material 2 from: Weigand AM, Macher J-N (2018) A DNA metabarcoding protocol for hyporheic freshwater meiofauna: Evaluating highly degenerate COI primers and replication strategy. Metabarcoding and Metagenomics 2: e26869. https://doi.org/10.3897/mbmg.2.26869
Figure S1: Overview of sediment characteristics :
Supplementary material 4 from: Weigand AM, Macher J-N (2018) A DNA metabarcoding protocol for hyporheic freshwater meiofauna: Evaluating highly degenerate COI primers and replication strategy. Metabarcoding and Metagenomics 2: e26869. https://doi.org/10.3897/mbmg.2.26869
Table S1: Sampling site coordinates :
Supplementary material 1 from: Weigand AM, Macher J-N (2018) A DNA metabarcoding protocol for hyporheic freshwater meiofauna: Evaluating highly degenerate COI primers and replication strategy. Metabarcoding and Metagenomics 2: e26869. https://doi.org/10.3897/mbmg.2.26869
DNA extraction protocol :
Supplementary material 1 from: {"en": "Kolter A, Gemeinholzer B (2021) Internal transcribed spacer primer evaluation for vascular plant metabarcoding. Metabarcoding and Metagenomics 5: e68155. https://doi.org/10.3897/mbmg.5.68155"}
Supplementary files
Supplementary material 8 from: Macher T-H, Schütz R, Yildiz A, Beermann AJ, Leese F (2023) Evaluating five primer pairs for environmental DNA metabarcoding of Central European fish species based on mock communities. Metabarcoding and Metagenomics 7: e103856. https://doi.org/10.3897/mbmg.7.103856
Unmodified TaXon tables of each primer pair
Supplementary material 7 from: Macher T-H, Schütz R, Yildiz A, Beermann AJ, Leese F (2023) Evaluating five primer pairs for environmental DNA metabarcoding of Central European fish species based on mock communities. Metabarcoding and Metagenomics 7: e103856. https://doi.org/10.3897/mbmg.7.103856
Protocol for the adapted NucleoMag Tissue Kit
Supplementary material 5 from: Macher T-H, Schütz R, Yildiz A, Beermann AJ, Leese F (2023) Evaluating five primer pairs for environmental DNA metabarcoding of Central European fish species based on mock communities. Metabarcoding and Metagenomics 7: e103856. https://doi.org/10.3897/mbmg.7.103856
List of all ambiguous assignments
Supplementary material 11 from: Macher T-H, Schütz R, Yildiz A, Beermann AJ, Leese F (2023) Evaluating five primer pairs for environmental DNA metabarcoding of Central European fish species based on mock communities. Metabarcoding and Metagenomics 7: e103856. https://doi.org/10.3897/mbmg.7.103856
Python scripts used in this study
Supplementary material 6 from: Macher T-H, Schütz R, Yildiz A, Beermann AJ, Leese F (2023) Evaluating five primer pairs for environmental DNA metabarcoding of Central European fish species based on mock communities. Metabarcoding and Metagenomics 7: e103856. https://doi.org/10.3897/mbmg.7.103856
List of over splitting rates per primer pair for each detected species
Supplementary material 10 from: Macher T-H, Schütz R, Yildiz A, Beermann AJ, Leese F (2023) Evaluating five primer pairs for environmental DNA metabarcoding of Central European fish species based on mock communities. Metabarcoding and Metagenomics 7: e103856. https://doi.org/10.3897/mbmg.7.103856
Processed and manually curated TaXon tables of each primer pair
A comprehensive and comparative evaluation of primers for metabarcoding eDNA from fish
<p>Accurate assessments of fish species diversity and community composition are essential for understanding fish ecology and conservation management. Environmental DNA (eDNA) metabarcoding has become an integrated method for monitoring fish species. The accuracy and efficacy of eDNA metabarcoding relies heavily on the choice of primers used for PCR amplification. A wide selection of metabarcoding primers for fish has been developed; however, there exists no comprehensive and comparative evaluation of their amplification or taxonomic classification of a rich diversity of fish species, which hinders informed decisions regarding their suitability for different study systems.</p> <p>Here, we reviewed the literature and compiled a list of 22 primer sets for eDNA-based metabarcoding analysis of teleost fish, the performance of which was compared using <i>in silico</i> PCR, followed by <i>in vitro</i> metabarcoding analysis using eDNA from waterbodies in Beijing, which harbour a high number of freshwater fish species.</p> <p>We found that the primers showed considerable differences in the amplified taxonomic ranges and proportions, fish taxa richness, species discrimination power, and fish community compositions, both <i>in silico</i> and <i>in vitro</i>. The number of fish taxa detected from eDNA by the primer sets varied from 0 to 66. Primers targeting the 12S rRNA gene generally detected greater fish diversity than those targeting the 16S rRNA or COI genes, while primers targeting the cytochrome b gene amplified the fewest fish taxa <i>in vitro</i>. 4. Regarding target genes, 12S primers generally outperformed other primers in terms of amplified fish diversity. The results of <i>in silico</i> PCR and <i>in vitro</i> tests were not always in agreement, suggesting that primer choice for biodiversity surveys should not be based solely on <i>in silico</i> evaluation. The use of different primers can qualitatively and quantitatively affect the detected biodiversity and these effects should be considered in experimental design and data interpretation. These results will assist with primer selection for eDNA-based fish surveys, and consequently support conservation of freshwater biodiversity.</p>
SABR PRIMER - Evaluating Stereotactic Ablative Radiotherapy for Primary and Regional Breast Tumors
ClinicalTrials.gov study NCT06937281. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
A comprehensive and comparative evaluation of primers for metabarcoding eDNA from fish
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