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78 results for “reference sequence”
Simulated data from: Reference-free assembly of long-read transcriptome sequencing data with RNA-Bloom2
<p><span>Long-read sequencing technologies have improved significantly since their emergence. Their read lengths, potentially spanning entire transcripts, is advantageous for reconstructing transcriptomes. Existing long-read transcriptome assembly methods are primarily reference-based and to date, there is little focus on reference-free transcriptome assembly. We introduce RNA-Bloom2, a reference-free assembly method for long-read transcriptome sequencing data. </span>RNA-Bloom2 is available on GitHub at: <a href="https://github.com/bcgsc/RNA-Bloom">https://github.com/bcgsc/RNA-Bloom</a>.</p> <p><span>We benchmarked the assembly quality and the computational performance of RNA-Bloom2 on simulated data. We prepared two mouse simulated datasets with Trans-NanoSim</span><span> for the cDNA and dRNA sequencing protocols model on experimental ONT data</span><span>. The datasets were simulated </span><span>based on the mouse ENSEMBL annotation for GRCm39.</span><span> To investigate the effect of sequencing depth, we subsampled each dataset to 2, 10, and 18 million reads, resulting in a total of six sets of reads for our benchmarking experiments. Using the simulated data, w</span><span>e showed that the transcriptome assembly quality of RNA-Bloom2 is competitive to those of reference-based methods.</span></p>
Control panel created from 30-40 Nanopore or PacBio HiFi sequencing data from the Human Pangenome Reference Consortium
<p>This is control panel for <a href="https://github.com/friend1ws/nanomonsv">nanomonsv</a> software, which is expected to exclude many false positives as well as improve computational cost. This is made by aligning 30-40 Nanopore or PacBio HiFi sequencing data from Human Pangenome Reference Consortium (HPRC) to the GRCh38 or CHM13 reference genomes with <a href="https://github.com/lh3/minimap2">minimap2</a> version 2.24.</p> <p><strong>When you use these control panels and publish, do not forget to credit to <a href="https://humanpangenome.org/data-use-protocol/">HPRC</a>!</strong></p> <div> <div> <div> <p>Reference genomes:</p> <ul> <li>GRCh38: <a href="https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/001/405/GCA_000001405.15_GRCh38/seqs_for_alignment_pipelines.ucsc_ids/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.gz">Download GRCh38</a></li> <li>CHM13: <a href="https://s3-us-west-2.amazonaws.com/human-pangenomics/T2T/CHM13/assemblies/analysis_set/chm13v2.0_maskedY_rCRS.fa.gz">Download CHM13</a> <div> <div> <div> <div> </div> </div> </div> </div> </li> </ul> </div> </div> </div>
CO1 reference sequences dataset of the sedDNA Chironomidae study by Blattner et al. 2024
<p>This dataset contains the CO1 reference sequences used to identify the sedDNA metabarcoding reads in the study entitled "Sediment core DNA-Metabarcoding and chitinous remain identification: Integrating complementary methods to characterise Chironomidae biodiversity in lake sediment archives", which was submitted to Molecular Ecology Resources.</p>
The miRBase release 22 reference files: stem-loop sequences (hairpins) and mature miRNA sequences
<pre>The miRBase Sequence Database -- Release 22 ------------------------------------------- The miRBase database provides a searchable online repository for published microRNA sequences and associated annotation. miRBase also provides a gene naming and nomenclature function in the miRBase Registry. Release 22 of the database contains 38589 entries representing hairpin precursor miRNAs, expressing 48885 mature miRNA products, in 271 species. The data are freely available to all through the web interface at http://www.mirbase.org/ and in flatfile form from ftp://mirbase.org/pub/mirbase/. </pre>
MMSEQS meets AntiRef90: reference clusters of human antibody sequences
<p>This data set contains pre-computed mmseqs databases for the antiref fasta files created by <em>Briney et al.</em> </p> <p>Please cite the original work if you use any of the databases provided here.</p> <p>Sources:</p> <ul> <li><a href="https://github.com/brineylab/antiref">Antiref GitHub</a></li> <li><a href="../records/7474336">Antiref Zenodo</a></li> <li><a href="https://academic.oup.com/bioinformaticsadvances/article/3/1/vbad109/7247530?login=true">Antiref Paper</a></li> </ul> <p> </p> <p>The mmseqs databases were created as follows:</p> <p> </p> <p>```</p> <p>aria2x -x16 -s16 --input-file antiref_links.txt<br>snakemake -s antiref_mmseqs.smk --jobs 1 --cores 1 --local-cores 250</p> <p>```</p> <p> </p> <p>Please check the summary repository for the fasta files and snakemake files. In this sub repo we only store the antiref files matching the title of the repo.</p> <p> </p>
DNA sequences of transgenes detected via environmental DNA (raw ABI files, processed FASTA files, and reference alignments)
We demonstrate that simple, non-invasive environmental DNA (eDNA) methods can detect transgenes of genetically modified (GM) animals from terrestrial and aquatic sources in invertebrate and vertebrate systems. We detected transgenic fragments between 82-234 bp through targeted PCR amplification of environmental DNA extracted from food media of GM fruit flies (<i>Drosophila melanogaster</i>), feces, urine, and saliva of GM laboratory mice (<i>Mus musculus</i>), and aquarium water of GM tetra fish (<i>Gymnocorymbus ternetzi</i>). With rapidly growing accessibility of genome-editing technologies such as CRISPR, the prevalence and diversity of GM animals will increase dramatically. GM animals have already been released into the wild with more releases planned in the future. eDNA methods have the potential to address the critical need for sensitive, accurate, and cost-effective detection and monitoring of GM animals and their transgenes in nature.
SARS-COV-2 genomic sequences used as references for the NASCarD method
<p>A set of 9 SARS-CoV-2 genome sequences used as reference in the NASCarD process.</p>
Massive Chinese domestic pigs provide missing sequences in reference genome and reveal non-coding sequence variations regulating gene expression across Eurasian boars
<p>This dataset contains novel sequences in Chinese domestic pigs but is absent in Sscrofa 11.1 reference genome. The detailed information for each file is recorded in the README file.</p>
The Genomic Reference Resource for African Cattle: genome sequences and high-density array variants.
<p><em>The diversity in genome resources is fundamental to designing genomic strategies for local breed improvement and utilisation. These resources also support gene discovery and enhance our understanding of the mechanisms of resilience with applications beyond local breeds. We report here the genome sequences of 573 samples (198 new genomes) and high-density (HD) array genotyping of 1,082 samples (537 new samples) from indigenous African cattle populations. The new sequences have an average genome coverage of ~30X, three times higher than the average (~10X) of the over 300 sequences already in the public domain. Following variant quality checks, we identified approximately 32.4 million sequence variants and 661,943 HD autosomal variants mapped to the Bos taurus reference genome (ARS-UCD1.2). The new datasets were generated as part of the Centre for Tropical Livestock Genetic and Health (CTLGH) Genomic Reference Resource for African Cattle (GRRFAC) initiative, which aspires to facilitate the generation of this livestock resource. We hope this resource will be utilised by the global scientific community and breeders for sustainable global livestock improvement.</em></p>
DNA sequences of transgenes detected via environmental DNA (raw ABI files, processed FASTA files, and reference alignments)
Open the record for dataset details and reuse information.
Simulated data from: Reference-free assembly of long-read transcriptome sequencing data with RNA-Bloom2
Open the record for dataset details and reuse information.
List of tissue specimens of Hipposideros spp. used for cytochrome b sequencing and phylogenetic inference, with geographical data. Voucher refers to the location and/or accession number of the voucher, and tissue collection refers to the collection where the tissue is kept, and accession or collector numbers. Acronyms are as follows: Estación Biológica de DoZana, Sevilla, Spain (EBD), South Australia Museum, Adelaide, Australia (SAM), Senckenberg Museum, Frankfurt am Main, Germany (SMF), Instituto de Ecología, Xalapa, México (IEX), Louisiana State University, Baton Rouge, Louisiana, USA (LSU), Charles M. Francis, Canadian Wildlife Service, Ottawa, Ontario, Canada (CMF), Lao Department of Forestry, Vientiane, Lao PDR, no catalogued (LAO). Next column indicates GenBank accession numbers. More information about the specimens is available in the GenBank records in A new species of bat of the Hipposideros bicolor group (Chiroptera: Hipposideridae) from Central Laos, with evidence of convergent evolution with Sundaic taxa
List of tissue specimens of Hipposideros spp. used for cytochrome b sequencing and phylogenetic inference, with geographical data. Voucher refers to the location and/or accession number of the voucher, and tissue collection refers to the collection where the tissue is kept, and accession or collector numbers. Acronyms are as follows: Estación Biológica de DoZana, Sevilla, Spain (EBD), South Australia Museum, Adelaide, Australia (SAM), Senckenberg Museum, Frankfurt am Main, Germany (SMF), Instituto de Ecología, Xalapa, México (IEX), Louisiana State University, Baton Rouge, Louisiana, USA (LSU), Charles M. Francis, Canadian Wildlife Service, Ottawa, Ontario, Canada (CMF), Lao Department of Forestry, Vientiane, Lao PDR, no catalogued (LAO). Next column indicates GenBank accession numbers. More information about the specimens is available in the GenBank records
Whole genome re-sequencing workshop data: fastq files and reference genomes
<p> </p> <p>Whole genome re-sequencing data analysis workshop datasets. The files are necessary inputs for the workshop in https://github.com/PoODL-CES/Genomics_learning_workshop</p> <p>Tools and scripts listed in the https://github.com/PoODL-CES/Genomics_learning_workshop repository.</p> <p> </p> <p>These are subsampled fastq files from:<br><br>Khan, A., Patel, K., Shukla, H., Viswanathan, A., van der Valk, T., Borthakur, U., Nigam, P., Zachariah, A., Jhala, Y.V., Kardos, M. and Ramakrishnan, U., 2021. Genomic evidence for inbreeding depression and purging of deleterious genetic variation in Indian tigers. <em>Proceedings of the National Academy of Sciences</em>, <em>118</em>(49), p.e2023018118.</p> <p>The reference genome is from :</p> <p>Shukla, H., Suryamohan, K., Khan, A., Mohan, K., Perumal, R.C., Mathew, O.K., Menon, R., Dixon, M.D., Muraleedharan, M., Kuriakose, B. and Michael, S., 2023. Near-chromosomal de novo assembly of Bengal tiger genome reveals genetic hallmarks of apex predation. <em>GigaScience</em>, <em>12</em>, p.giac112.</p> <p> </p> <p>The reference has been indexed using:</p> <p>bwa index <a target="_blank" rel="noopener noreferrer">GCA_021130815.1_PanTigT.MC.v3_genomic.fna</a></p> <p> </p>
Amplicon_sorter: a tool for reference-free amplicon sorting based on sequence similarity and for building consensus sequences
<p>Oxford Nanopore Technologies (ONT) is a third-generation sequencing technology that is gaining popularity in ecological research for its portable and low-cost sequencing possibilities. Although the technology excels at long-read sequencing, it can also be applied to sequence amplicons. The downside of ONT is the low quality of the raw reads. Hence, generating a high-quality consensus sequence is still a challenge. We present Amplicon_sorter, a tool for reference-free sorting of ONT sequenced amplicons based on their similarity in sequence and length and for building solid consensus sequences.</p>
FIGURE. Multilocus phylogenetic tree inferred from Bayesian analysis based on the combined TEF1-α and ACT sequences. Bayesian posterior probabilities are indicated next to the nodes. The tree was rooted with Cladosporium herbarum CBS 121621. The species in this study are indicated in bold. Types of species are indicated after the culture collection number (T = ex-type, ex-epitype, ex-neotype, or reference strain). in Six new species of Cladosporium associated with decayed leaves of native bamboo (Bambusoideae) in a fragment of Brazilian Atlantic Forest
FIGURE. Multilocus phylogenetic tree inferred from Bayesian analysis based on the combined TEF1-α and ACT sequences. Bayesian posterior probabilities are indicated next to the nodes. The tree was rooted with Cladosporium herbarum CBS 121621. The species in this study are indicated in bold. Types of species are indicated after the culture collection number (T = ex-type, ex-epitype, ex-neotype, or reference strain).
FIGURE. (Continued) Multilocus phylogenetic tree inferred from Bayesian analysis based on the combined TEF1-α and ACT sequences. Bayesian posterior probabilities are indicated next to the nodes. The tree was rooted with Cladosporium herbarum CBS 121621. The species in this study are indicated in bold. Types of species are indicated after the culture collection number (T = ex-type, ex-epitype, exneotype, or reference strain). in Six new species of Cladosporium associated with decayed leaves of native bamboo (Bambusoideae) in a fragment of Brazilian Atlantic Forest
FIGURE. (Continued) Multilocus phylogenetic tree inferred from Bayesian analysis based on the combined TEF1-α and ACT sequences. Bayesian posterior probabilities are indicated next to the nodes. The tree was rooted with Cladosporium herbarum CBS 121621. The species in this study are indicated in bold. Types of species are indicated after the culture collection number (T = ex-type, ex-epitype, exneotype, or reference strain).
A tufA reference sequence library for the green algal genus Caulerpa (Bryopsidales)
<p>A 1230 nucleotide <em>tuf</em>A gene sequence alignment (in fasta format) of 89 <em>Caulerpa</em> species and nine '<em>Pseudochlorodesmis</em>' species, a Maximum Likelihood phylogeny (pdf file) based on this alignment, and an Excel file with listing details about all taxa included. These were first published in Draisma & Sauvage (2024): Jeju Journal of Islands Sciences 1: 1-11.</p> <p>https://www.jjis.or.kr/articles/xml/VxXM/</p> <p> </p>
Fig. 1 in Identification of Twelve Species of Coccinellidae (Coleoptera) Predatory on Melanaphis sacchari (Zehntner) (Hemiptera: Aphididae) in Mexico, and Submission of Reference Coi Sequences
Fig. 1. Coccinellid predators of Melanaphis sacchari in cultivated sorghum in Mexico. A) Brachiacantha decora, B) Coccinella septempunctata, C) Coleomegilla maculata lengi, D) Cycloneda sanguinea sanguinea, E) Diomus roseicollis, F) Diomus terminatus, G) Exochomus childreni guexi, H) Harmonia axyridis, I) Hippodamia convergens, J) Olla v-nigrum, K) Scymnus (Pullus) dozieri, and L) Scymnus (Pullus) loewii.
thus and genetic % 1 than less indicates Green . ) kb 15 . ca ( Ixodes of ) individuals 40 ( species bold 34 in of are genomes study present mitochondrial the in entire sequenced the Species among. differences reference for genetic, species ) % ( same Pairwise the. 3 from FIGURE sequences in A new subgenus, Australixodes n. subgen. (Acari: Ixodidae), for the kiwi tick, Ixodes anatis Chilton, 1904, and validation of the subgenus Coxixodes Schulze, 1941 with a phylogeny of 16 of the 22 subgenera of Ixodes Latreille, 1795 from entire mitochondrial genome sequences
thus and genetic % 1 than less indicates Green . ) kb 15 . ca ( Ixodes of ) individuals 40 ( species bold 34 in of are genomes study present mitochondrial the in entire sequenced the Species among. differences reference for genetic, species ) % ( same Pairwise the. 3 from FIGURE sequences
Fig. 1 in High-Throughput Sequencing for Life-History Sorting and for Bridging Reference Sequences in Marine Gerromorpha (Insecta: Heteroptera)
Fig. 1. Representative images of Halobates species collected (rows), and their various life stages (columns). (Top row) Halobates calyptus: nymph (A), adult male (B), and adult female (C); (middle row) Halobates hayanus: nymph (D), adult male (E), and adult female (F); (bottom row) Halobates maculatus: nymph (G), adult male (H) and adult female (I). All images are scaled to size, and black scale bars represent 1 mm.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.