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11,105 results for “single cell”

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zenodo44/100

A Single-Cell Tumor Immune Atlas for Precision Oncology

<p><strong>Publication&nbsp;version of the Single-Cell Tumor Immune Atlas</strong></p> <p>This upload contains:</p> <ul> <li><strong>TICAtlas.rds:</strong>&nbsp;an rds file containing a Seurat object&nbsp;with the whole Atlas</li> <li><strong>TICAtlas.h5ad:</strong>&nbsp;an h5ad file with the whole Atlas</li> <li><strong>TICAtlas_downsampled.rds:</strong>&nbsp;an rds file containing a downsampled version of the Seurat object of the whole Atlas</li> <li><strong>TICAtlas_downsampled.h5ad:</strong>&nbsp;an rds file containing a downsampled version of the Seurat object of the whole Atlas</li> <li><strong>TICAtlas_metadata.csv:&nbsp;</strong>a comma-separated text file with the metadata for each of the cells</li> </ul> <p>All the files contain the following patient/sample metadata variables:</p> <ul> <li>patient: assigned patient identifiers</li> <li>nCountRNA and nFeatureRNA: number of UMIs and genes per cell</li> <li>percent.mt: percentage of mitochondrial genes</li> <li>gender: the patient&#39;s gender (male/female/unknown)</li> <li>source: dataset of origin</li> <li>subtype: cancer type (abbreviations as indicated in the preprint)</li> <li>kmeans_cluster: patients clusters, NA if filtered out before clustering</li> <li>lv1 and lv2: annotated cell type for each of the cells, two level annotation (lv2 has more cell types)</li> </ul> <pre>&nbsp;</pre> <p>If you have any issues with the metadata (i.e. unexpected factors, NA values...) you can use the <strong>TICAtlas_metadata.csv </strong>file.</p> <p>For more information,&nbsp;<a href="https://genome.cshlp.org/content/early/2021/09/21/gr.273300.120.">read our paper</a>,&nbsp;<a href="https://github.com/Single-Cell-Genomics-Group-CNAG-CRG/Tumor-Immune-Cell-Atlas">check our GitHub</a>&nbsp;and our <a href="https://singlecellgenomics-cnag-crg.shinyapps.io/TICA/">ShinyApp</a>.</p> <p>h5ad files can be read with Python using&nbsp;<a href="https://scanpy.readthedocs.io/en/stable/">Scanpy</a>, rds files can be read in R using&nbsp;<a href="https://satijalab.org/seurat/">Seurat</a>. For format conversion between AnnData and Seurat we recommend&nbsp;<a href="https://mojaveazure.github.io/seurat-disk/">SeuratDisk</a>. For other single-cell data formats you can use&nbsp;<a href="https://github.com/cellgeni/sceasy">sceasy</a>.</p>

opencc-by-4.0Sep 2020View details →
zenodo44/100

Evaluating the influence of structural properties on proximity metric performance in single cell RNA-seq data - Datasets

<p>Includes raw and processed copies of the scRNA-seq datasets used for the paper: &#39;<strong>How does data structure impact cell-cell similarity? Evaluating the influence of structural properties on proximity metric performance in single cell RNA-seq data.&#39;</strong></p> <p><strong>Real scRNA-seq.zip </strong>contains the Abundant (subset1) and Rare (subset 2) subsets generated to represent discretely structured datasets (sourced from<strong> </strong> Wegmann et al. 2019) and the continuously structured data (sourced from Popescu et al. 2019).</p> <p><strong>Simulated scRNA-seq.zip</strong> contains the Abundant, Moderately-Rare and Ultra-Rare subsets for discretely and continuously structured datasets. All data was simulated using the PROSSTT package in Python 3.8, as well as the dataset containing the labels to re-produce Figure 3 of the manuscript.</p> <p><strong>Results.zip </strong>contains the results for all datasets from the full analysis, in a pickled python dictionary. Code to read in and visualise results is available on the projects github</p> <p>The scripts for the dataset generation, processing and visualisation of results are available at <a href="https://github.com/Ebony-Watson/scProximitE">our github for the scProcimitE package</a>, and documentation is available <a href="https://ebony-watson.github.io/scProximitE/">here</a>.</p>

opencc-by-4.0Apr 2022View details →
zenodo44/100

Data files: Single-cell RNA profiling of Plasmodium vivax-infected hepatocytes reveals parasite- and host- specific transcriptomic signatures and therapeutic targets

<p>Scripts, preprocessed count matrices, and single-cell data objects generated&nbsp;in&nbsp;<strong>&ldquo;Single-cell RNA profiling of&nbsp;<em>Plasmodium vivax</em><em>-</em>infected hepatocytes reveals parasite- and host- specific transcriptomic signatures&nbsp;and therapeutic targets&rdquo;&nbsp;</strong></p>

opencc-by-4.0Jul 2022View details →
zenodo44/100

Single-cell analysis of megakaryopoiesis in peripheral CD34+ cells: insights into ETV6-related thrombocytopenia

<p>This repository contains necessary files for reproducing the analysis in Bigot et al, 2023. The instructions for reproducing the analysis are given in github (https://github.com/poggiteam/ETV6_2020).</p>

opencc-by-4.0Sep 2022View details →
zenodo44/100

microSPLiT single-cell and bulk transcriptomes analysed with STAR - Pseudomonas putida KT2440/pKJK5

<h3>Description of the data and file structure</h3> <p>Data are displayed as 2 files</p> <p><strong>1. Bulk transcriptomics results (Bulk_STAR.csv)</strong></p> <p>STAR processed data combined in a gene x sample table</p> <p><strong>2. microSPLiT single-cell results (microSPLiT_STARsolo.xlsx)</strong></p> <p>STARsolo processed data combined as sublibraries&rsquo; gene associated transcript numbers (UMIs) per cell for the control (E1) and experiment (E2) sublibraries (F1-8) - (1 sublibrary per table).</p> <div> <p>&nbsp;</p> </div>

opencc-by-4.0May 2024View details →
zenodo44/100

Development of ferret immune repertoire reference resources and single-cell-based high- throughput profiling assays

<p>We performed long read transcriptome sequencing of ferret splenocyte and lymph node samples full-length, non-chimeric circular consensus sequencing (CCS) reads&nbsp;to obtain over 120,000 high-quality&nbsp;immunoglobin (Ig) and T cell receptor (TCR) transcripts.</p>

opencc-by-4.0May 2024View details →
zenodo44/100

Single-cell transcriptomic profiling unveils dysregulation of cardiac progenitor cells and cardiomyocytes in a mouse model of maternal hyperglycemia

<p>Congenital heart disease (CHD) is the most prevalent structural malformations of the heart affecting &sim;1% of live births. To date, both damaging genetic variations and adverse environmental exposure such as maternal diabetes have been found to cause CHD. Clinical studies show &sim;fivefold higher risk of CHD in the offspring of mothers with pregestational diabetes. Maternal pregestational diabetes affects the gene regulatory networks key to proper cardiac development in the fetus. However, the cell-type specificity of these gene regulatory responses to maternal diabetes and their association with the observed cardiac defects in the fetuses remains unknown. To uncover the transcriptional responses to maternal diabetes in the early embryonic heart, we used an established murine model of pregestational diabetes. In this model, we have previously demonstrated an increased incidence of CHD. Here, we show maternal hyperglycemia (matHG) elicits diverse cellular responses during heart development by single-cell RNA-sequencing in embryonic hearts exposed to control and matHG environment. Through differential gene-expression and pseudotime trajectory analyses of this data, we identified changes in lineage specifying transcription factors, predominantly affecting Isl1+ second heart field progenitors and Tnnt2+cardiomyocytes with matHG. Using in vivo cell-lineage tracing studies, we confirmed that matHG exposure leads to impaired second heart field-derived cardiomyocyte differentiation. Finally, this work identifies matHG-mediated transcriptional determinants in cardiac cell lineages elevate CHD risk and show perturbations in Isl1-dependent gene-regulatory network (Isl1-GRN) affect cardiomyocyte differentiation. Functional analysis of this GRN in cardiac progenitor cells will provide further mechanistic insights into matHG-induced severity of CHD associated with diabetic pregnancies.</p>

opencc-by-4.0Jun 2024View details →
zenodo44/100

Dataset of "Single-Cell RNA-Seq Reveals Transcriptional Heterogeneity in Latent and Reactivated HIV-infected Cells"

<p><strong>Detailed quantitative analysis of GFP expression in SAHA and TCR-treated cells &amp; Computational analysis of&nbsp;bulk and single-cell RNA-Seq data.</strong></p> <p>&nbsp;</p> <p><em><strong>Detailed quantitative analysis of GFP expression in SAHA and TCR-treated cells.</strong></em></p> <p>Cells were prepared for single cell analysis at the Genome Technology Facility (GTF) of the University of Lausanne. Cells were loaded on Fluidigm C1 IFC plates (5-10 &mu;m), with run ID smart33, smart34 and smart35, corresponding to untreated, SAHA- and TCR-treated conditions respectively. After single cell capture on the Fluidigm C1 IFC plate, each chamber was inspected visually by microscopy and pictures were captured with a Zeiss Axiovert 200 M fluorescence microscope equipped with a Roper Scientific CoolSnap HQ camera using a Plan-Neofluar 10X lens (smart34 run) or 20X lens (for smart35 run). For each capture chamber, pictures in bright field and FITC channel were taken with the MetaMorph 6.3 software. Picture analysis was then performed using ImageJ 1.50b software (open access software: website). Brightness and contrast were adjusted for qualitative assessment of the pictures.</p> <p><em><strong>Computational analysis of&nbsp;bulk and single-cell RNA-Seq data.</strong></em></p> <p>Upon bulk or single cell isolation, RNA extraction and library preparation was performed according to Illumina protocols. Bulk and single-cell RNA-Seq data analysis are detailed here.</p> <p>&nbsp;</p> <p>Linked to the paper published in Cell Reports (doi:10.1016/j.celrep.2018.03.102):&nbsp;</p> <p><strong>Single-Cell RNA-Seq Reveals Transcriptional Heterogeneity&nbsp;in Latent and Reactivated HIV-infected Cells</strong></p> <p>Despite effective treatment, HIV can persist in latent reservoirs, which represent a major obstacle towards HIV eradication. Targeting and reactivating latent cells is challenging due to the heterogeneous nature of HIV infected cells. Here, we used a primary model of HIV latency and single-cell RNA sequencing to characterize transcriptional heterogeneity during HIV latency and reactivation. Our analysis identified transcriptional programs leading to successful reactivation of HIV expression.</p> <p>&nbsp;</p> <p>&nbsp;</p>

opencc-by-4.0Feb 2018View details →
zenodo44/100

Single-cell RNA-seq of breast cancer infiltrating T cells (case 1)

<p>Single cell suspensions were generated from two individual TNBC primary&nbsp;tumor samples (this&nbsp;entry contains case 2) and the viable cells were FACS sorted for CD3<sup>+</sup> T cells.&nbsp;Sorted cells were then counted and assessed for viability. Single cell library preparation was carried out as per the 10X Genomics Chromium Single cell protocol for the v2 reagent kit (10X Genomics, Pleasanton, CA, USA). Cell suspensions were loaded onto a Chromium Single Cell Chip along with the reverse transcription (RT) mastermix and single cell 3&rsquo; gel beads. Following generation of single cell gel bead-in-emulsions (GEMs), reverse transcription was performed using a C1000 Touch Thermal Cycler with a Deep Well Reaction Module (Bio-Rad Laboratories, Hercules, CA, USA). Amplified cDNA was purified using SPRIselect beads (Beckman Coulter, Lane Cove, NSW, Australia) and sheared to approximately 200bp with a Covaris S2 instrument (Covaris, Woburn, MA, USA) using the manufacturer&rsquo;s recommended parameters. Sequencing libraries were generated with unique sample indices (SI) for each sample. Libraries were sequenced on an Illumina HiSeq 2500 High Output Mode using V4 clustering and sequencing chemistry.</p> <p>This dataset&nbsp;contains&nbsp;the raw .bcl files.</p>

opencc-by-4.0Jun 2018View details →
zenodo44/100

Single-cell RNA-seq of breast cancer infiltrating T cells (case 2)

<p>Single cell suspensions were generated from two individual TNBC primary&nbsp;tumor samples (this&nbsp;entry contains case 1) and the viable cells were FACS sorted for CD3<sup>+</sup> T cells.&nbsp;Sorted cells were then counted and assessed for viability. Single cell library preparation was carried out as per the 10X Genomics Chromium Single cell protocol for the v2 reagent kit (10X Genomics, Pleasanton, CA, USA). Cell suspensions were loaded onto a Chromium Single Cell Chip along with the reverse transcription (RT) mastermix and single cell 3&rsquo; gel beads (this sample was divided into two channels). Following generation of single cell gel bead-in-emulsions (GEMs), reverse transcription was performed using a C1000 Touch Thermal Cycler with a Deep Well Reaction Module (Bio-Rad Laboratories, Hercules, CA, USA). Amplified cDNA was purified using SPRIselect beads (Beckman Coulter, Lane Cove, NSW, Australia) and sheared to approximately 200bp with a Covaris S2 instrument (Covaris, Woburn, MA, USA) using the manufacturer&rsquo;s recommended parameters. Sequencing libraries were generated with unique sample indices (SI) for each sample. Libraries were sequenced on an Illumina HiSeq 2500 High Output Mode using V4 clustering and sequencing chemistry.</p> <p>This dataset&nbsp;contains&nbsp;the raw .bcl files.</p>

opencc-by-4.0Jun 2018View details →
zenodo44/100

AOSLO Single Cell Blood Flow - Raw Data (eLife paper: Joseph et al. 2019)

<p>Raw AOSLO videos of eLife paper Joseph et al. 2019 - &#39;Imaging single-cell blood flow in the smallest to largest vessels in the living retina&#39;.&nbsp;<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__doi.org_10.7554_eLife.45077&amp;d=DwMFaQ&amp;c=kbmfwr1Yojg42sGEpaQh5ofMHBeTl9EI2eaqQZhHbOU&amp;r=EdsTL7DuEvOHun7eVBmBd9sxUuPhDEmdFDf0tlkKUO4&amp;m=56MyNrE_d-v6PSU7Go9NePVOrIpAvWHBaC8wVvgs3_k&amp;s=G7SkWT-fSV9d3SJmpWEUUGp2a6mGotbG-uAwNZftpIo&amp;e=">https://doi.org/10.7554/eLife.45077</a>&nbsp;. For additional data or questions, please contact author Aby Joseph (aby.joseph@rochester.edu, dreamworks1991@gmail.com)</p>

opencc-by-4.0May 2019View details →
zenodo44/100

Single Cell RNAseq of Mouse Testis

<p>This is the processed data from the publication:&nbsp;&quot;Unified single-cell analysis of testis gene regulation and pathology in 5 mouse strains&quot; (https://doi.org/10.1101/393769)</p> <p>The raw data is avaliable at GEO:&nbsp;GSE113293</p> <p>Associated software is at&nbsp;https://zenodo.org/badge/latestdoi/140632831</p> <p>SDA_objects.zip contains key tables required for many functions, download this to use the shiny app. Contents:</p> <ul> <li>cell_data: data.table containing metadata of the cells. 80 columns including cell id, SDA component cell scores, Tsne coordinates, pseudotime, experimental group etc.</li> <li>data: a sparse matrix of normalised read counts (20322 cells by 19262 genes)</li> <li>gene_annotations: data.table containing gene locations, enrichment vs other tissues from bulk data, infertility gene status</li> <li>GO_enrich: data.table containing gene ontology enrichments for each component, the genes enriched, p.values and enrichment values</li> <li>principal_curves: list of output of princurve() containing the pseudotime trajetories</li> <li>SDAresults: output of SDA run loaded using SDAtools::load_results()</li> </ul> <p>Other R objects include:</p> <ul> <li>QC_count_matrix: Sparse matrix of raw count values for QC cells and genes</li> <li>cell_imputation_AUCs: data.table of PRAC AUC values for unimputed (train), mean cell, SDA (predict), ICA, PCA, NNMF, and MAGIC</li> <li>HocomocoV11_motifProbs_matrix: Matrix of regulation probabilities from MotifFinder using fixed motifs from HocomocoV11 database</li> <li>motifFinder_denovo: list of results of MotifFinder denovo motifs from promoters regions of genes from each component</li> <li>motifFinder_denovo_fixed: list of results of MotifFinder using 125 denovo motifs with fixed motif on all genes.</li> <li>tomtom_matched_motifs: data.table of TOMTOM matches of denovo motifs, plus metadata</li> </ul>

opencc-by-4.0Aug 2018View details →
zenodo44/100

CellSIUS provides sensitive and specific detection of rare cell populations from complex single cell RNA-seq data: Codes and processed data

<p>Codes and processed data to reproduce the analysis discussed in:&nbsp;</p> <p>Wegmann <em>et Al.</em>,<strong> CellSIUS provides sensitive and specific detection of rare cell<br> populations from complex single cell RNA-seq data</strong>, Genome Biology 2019 (Accepted)<br> &nbsp;</p>

openapache2.0Jun 2019View details →
zenodo44/100

Genome alignments for the project "Whole transcriptome analysis of thousands of FACS-sorted single cells with the single cell nanoCAGE protocol" - Protocol optimization

<p>Genome alignments for data generated in the project &quot;<em>Whole transcriptome analysis of thousands of FACS-sorted single cells with the single cell nanoCAGE protocol &ndash; Optimization of the protocol.</em>&quot; Files names indicate unique identifiers of MOIRAI workflow runs, with the following structure: library name, dot, workflow ID (OP-WORKFLOW-CAGEscan-short-reads-v2.0.), dot, timestamp. The raw (FASTQ) data of each library is also deposited in Zenodo (<a href="https://doi.org/10.5281/zenodo.250156">10.5281/zenodo.250156</a>). Library names correspond to the following runs:</p> <ul> <li>&nbsp;NC33: 151007_M00528_0161_000000000-AEBDC</li> <li>&nbsp;NC37: 151204_M00528_0173_000000000-AEBEF</li> <li>&nbsp;NC38: 151211_M00528_0175_000000000-AE9PJ</li> <li>&nbsp;NC39: 160122_M00528_0185_000000000-AEB18</li> <li>&nbsp;NC42: 160302_M00528_0192_000000000-AELYK</li> </ul> <p>This data can be analysed using the &quot;CAGEr&quot; software package available from Bioconductor.&nbsp; The &quot;multiplex_files.zip&quot; file contains tables indicating which samples are biological replicates of each other or negative controls.</p>

opencc-zeroJul 2019View details →
zenodo44/100

Supplementary material: Efficient in vivo screening method for the identification of C4 photosynthesis inhibitors based on cell suspensions of the single-cell C4 plant Bienertia sinuspersici

<p>Data described in Minges et al. (2019) Efficient <em>in vivo</em> screening method for the identification of C<sub>4</sub> photosynthesis inhibitors based on cell suspensions of the single-cell C<sub>4</sub> plant <em>Bienertia sinuspersici</em>. doi: <a href="https://doi.org/10.3389/fpls.2019.01350">10.3389/fpls.2019.01350</a></p> <p>&nbsp;</p>

opencc-by-4.0Apr 2019View details →
zenodo44/100

Data associated with "Microbiota-derived metabolites inhibit Salmonella virulent subpopulation development by acting on single-cell behaviors"

<p>Data used for the publication Microbiota-derived metabolites inihibit Salmonella virulent subpopulation development by acting on single-cell behaviors. &nbsp;</p> <p>&nbsp;</p> <p>all_hi_2307202.csv &nbsp; &nbsp; &nbsp; Single-cell quantifications of Salmonella SPI-1 reporter cells grown in the presence of SCFAs.</p> <p>all_no_2307202.csv &nbsp; &nbsp; &nbsp;Single-cell quantifications of Salmonella SPI-1 reporter cells grown in the absence of SCFAs.</p> <p>odmeasurements.csv &nbsp; &nbsp; OD measurements of plate-reader assays of Salmonella SPI-1 reporter cells and controls grown in a range of SCFA conditions. &nbsp;</p> <p>gfpmeasurements.csv &nbsp; &nbsp;GFP measurements of plate-reader assays of Salmonella SPI-1 reporter cells and controls grown in a range of SCFA conditions. &nbsp;</p>

opencc-by-4.0Sep 2024View details →
zenodo44/100

VeloCycle-estimated cell cycle phases of single cells from a genome-scale perturb-seq performed in K562

<p>Continuous cell cycle phase position between 0 and 2&pi; estimated by <em>VeloCycle&nbsp;</em>(Lederer et al.,&nbsp;<em>Nature Methods</em> 2024) for single cells of the perturb-seq performed in the K562 CML cell line by Replogle et al., Cell 2022.&nbsp;</p> <p>Underlies the cell cycle imbalances inferred in Pulver &amp; Forey et al., 2024.</p> <p>Method manuscript exerpt:</p> <p>"For analysis on the genome-wide perturb-seq dataset of K562 cells (Replogle <em>et al.</em>, 2022), a transfer learning approach was applied. Condition-independent estimation of the periodic Fourier series components would be especially challenging on Perturb-seq knockdown conditions containing either (1) very few cells or (2) cells belonging to just one phase of the cell cycle. To infer accurate cell cycle phases for these cells, we first performed manifold-learning for 5,000 training steps to estimate the gene harmonic coefficients (&nu;0, &nu;1sin, &nu;1cos) on a larger set of non-targeting control (NT) K562 cells (75,328 cells), which are more evenly distributed throughout the various phases of the cell cycle. Next, we ran manifold-learning again for 5,000 training steps, but on the entire perturb-seq dataset of 1,971,608 cells and 4,127 gene knockdown conditions (with at least 75 cells per condition). This time, we conditioned <em>VeloCycle</em> on the gene harmonic coefficients learned in the first step. This allowed cells belonging to each stratified gene knockdown condition to be assigned to a position on the cell cycle manifold, while restricting those assignments such that they were based on gene expression patterns earned on a larger and more informative dataset (allowing for batch effect expression differences)."</p>

opencc-by-4.0Oct 2024View details →
zenodo44/100

Data from: Polymorphic tandem repeats shape single-cell gene expression across the immune landscape

<p>This dataset contains the association summary statistics (v0.1) for genome-wide tandem repeat (TR) expression quantitative trait (eQTL) analysis of TenK10K Phase 1 (https://doi.org/10.1101/2024.11.02.621562).&nbsp;</p> <p>Please access the README for a detailed description of file contents.&nbsp;</p> <p>&nbsp;</p>

opencc-by-4.0Oct 2024View details →
zenodo44/100

GWAS to single cell: Intersecting single-cell transcriptomics and genome wide association studies identifies crucial cell-populations and candidate genes for atherosclerosis.

<p><strong>Background</strong></p> <p>Genome-wide association studies (GWAS) have discovered hundreds of common genetic variants for atherosclerotic disease and cardiovascular risk factors. The translation of susceptibility loci into biological mechanisms and targets for drug discovery remains challenging. Intersecting genetic and gene expression data has led to identification of candidate genes. However, the assayed tissues are often non-diseased and heterogeneous in cell composition confounding the candidate prioritization. We collected single-cell transcriptomics (scRNA-seq) from atherosclerotic plaques and aimed to identify cell-type-specific expression of disease-associated genes.&nbsp;</p> <p>&nbsp;</p> <p><strong>Methods and Results</strong></p> <p>To identify disease-associated candidate genes, we applied gene-based analyses using GWAS summary statistics from 46 atherosclerotic, cardiometabolic, and other traits. Next we intersected these candidates with single-cell transcriptomics (scRNA-seq) to identify those genes that are specifically expressed in individual cell (sub)populations of atherosclerotic plaques. We derive an enrichment score and show that loci that associated with coronary artery disease demonstrated a prominent substrate in plaque smooth muscle cells (<em>SKI</em>, <em>KANK2</em>, <em>SORT1</em>), endothelial cells (<em>SLC44A1</em>, <em>ATP2B1</em>), and macrophages (<em>APOE</em>, <em>HNRNPUL1</em>). Further sub clustering of SMC-subtypes revealed genes in risk loci for coronary calcification specifically enriched in a synthetic cluster of SMCs. To verify the robustness of our approach, we used liver-derived scRNAseq-data and showed enrichment of circulating lipids-associated loci in hepatocytes.</p> <p><br> <strong>Conclusion</strong></p> <p>We confirm known gene-cell pairs relevant for atherosclerotic disease, and discovered novel pairs pointing to new biological mechanisms amenable for therapy. We present an intuitive single-cell transcriptomics driven workflow rooted in human large-scale genetic studies to identify putative candidate genes and affected cells associated with cardiovascular traits.</p> <p>&nbsp;</p>

opencc-by-4.0May 2021View details →
zenodo44/100

Symphony pre-built single-cell reference atlases

<p>Pre-built Symphony reference objects that can be downloaded and used to map new query datasets.</p> <p>The Symphony&nbsp;algorithm is used&nbsp;to perform reference mapping to these&nbsp;atlases.&nbsp;</p> <ul> <li>Preprint:&nbsp;<a href="https://www.biorxiv.org/content/10.1101/2020.11.18.389189v2">https://www.biorxiv.org/content/10.1101/2020.11.18.389189v2</a></li> <li>Usage:&nbsp;<a href="https://github.com/immunogenomics/symphony">https://github.com/immunogenomics/symphony</a></li> </ul> <p><strong>References available for download:</strong></p> <ol> <li>10x PBMCs Atlas (pbmcs_10x_reference.rds)</li> <li>Pancreatic&nbsp;Islet Cells Atlas (pancreas_plate-based_reference.rds)</li> <li>Fetal Liver Hematopoiesis Atlas (fetal_liver_reference_3p.rds)</li> <li>Healthy Fetal Kidney Atlas (kidney_healthy_fetal_reference.rds)</li> <li>T cell CITE-seq atlas (tbru_ref.rds)</li> <li>Cross-tissue Fibroblast Atlas (see <a href="https://sandbox.zenodo.org/record/772596#.YOqMiBNKhTY">here</a>)</li> <li>Cross-tissue Inflammatory Immune Atlas (<a href="https://sandbox.zenodo.org/record/888445#.YV9In2ZKijB">here</a>)</li> <li>Tabula Muris Senis (FACS) Atlas (TMS_facs_reference.rds)</li> </ol> <p>To read in a reference into R, one may simply execute: reference = readRDS(&#39;path/to/reference_name.rds&#39;)</p> <p>Note: To be able to map query datasets into the reference UMAP coordinates, you must also download the corresponding &#39;uwot_model&#39; file and set the reference$save_uwot_path.</p>

opencc-by-4.0Jul 2021View details →

ScienceDex guides

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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.

Compare curated datasets

Allen Brain Atlas

Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.

allen-brain-atlas
neuroscienceopenDocumentation, web resources, and API references are available online.
Last verified 2026-04-30Open record

Annotated Behaviour and Observability Dataset (ABODe)

ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.

abode-home-cage
behavioral-neuroscienceopenThe DataShare record exposes download links for annotations, documentation, license text, and the zipped per-snippet data directory.
Last verified 2026-04-30Open record

DANDI Archive for NWB datasets

DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.

dandi-nwb
electrophysiologyopenPublished Dandiset metadata and archive endpoints are available through the production DANDI API.
Last verified 2026-04-30Open record

International Brain Laboratory public data

The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.

ibl
behavioral-neuroscienceopenPublic sessions can be searched and loaded from the IBL public data server through ONE.
Last verified 2026-04-29Open record

OpenNeuro

OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.

openneuro
neuroscienceopenPublished datasets are available on demand over the internet.
Last verified 2026-04-29Open record