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102 results for “spatial imaging”
High spatial resolution satellite images for glacier outlines
<p>Two RapidEye satellite images acquired on Sep 13, 2013 and Sep 17, 2018. The two satellite images have a high spatial resolution of 5 m × 5 m and were used to derive the outlines of the Parlung No. 94 Glacier in years 2013 and 2018, respectively. The GaoFen-7 (GF-7) satellite image with a high spatial resolution of 3 m × 3 m acquired on Feb 26, 2021 was used to derive the outlines of the Dongkemadi Glacier in 2021</p>
SI_III_4_Spatialized metabolomic annotation combining MALDI imaging and molecular network
<p>Ces documents regroupent les données supplémentaires générés lors du développement méthdologique pour la création de réseaux moléculaires par MALDI-FT-ICR IMS. Un .ppt regroupe l'ensemble des cartographies ioniques spécifiques à chaque ion.</p>
Spatial transcriptomics images for papillary and anaplastic thyroid cancer IRIBHM dataset
<p>This dataset includes the image files for spatial transcriptomics data associated with the publication "Idiosyncratic and generic single nuclei and spatial transcriptional patterns in papillary and anaplastic thyroid cancers".</p>
Structural data and spectra for: Micro-optics in the cuticle of matt-green <em>Chrysina</em> beetles create spatially-projected images
Open the record for dataset details and reuse information.
Spatial-offset pump-probe imaging of nonradiative dynamics at optical resolution
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Spatial transcriptome mapping of the desmoplastic growth pattern of colorectal liver metastases by in situ sequencing - Image and In SItu Sequencing data
<p>Image and ISS data for Spatial transcriptome mapping of the desmoplastic growth pattern of colorectal liver metastases by in situ sequencing reveals a biologically relevant zonation of the desmoplastic rim.</p><p>Image data consists of:</p><ul><li>Nucler stain (DAPI)</li><li>Masks for liver, rim and tumor regions</li><li>H&E images of parallel tissue sections</li></ul><p>Gene and cluster marker data is collected in the <i>markers.h5ad</i> file which can be read using AnnData (<a href="https://anndata.readthedocs.io/en/latest/">https://anndata.readthedocs.io/en/latest/)</a>. </p><p> </p>
Raw IMC files for Spatial subsetting enables integrative modeling of oral squamous cell carcinoma multiplex imaging data.
<p>Raw MCD files for the Stanford cohort of oral squamous cell carcinoma patients in this publication:</p> <p>Spatial subsetting enables integrative modeling of oral squamous cell carcinoma multiplex imaging data (DOI:<span> <a href="https://doi.org/10.1016/j.isci.2023.108486" target="_blank" rel="noopener">10.1016/j.isci.2023.108486</a>).</span></p>
Test dataset for "Spatial Integration of Multi-Omics Data from Serial Sections using the novel Multi-Omics Imaging Integration Toolset"
<p>The uploaded tar file contains anonymized and reduced test data for the paper "Spatial Integration of Multi-Omics Data from Serial Sections using the novel Multi-Omics Imaging Integration Toolset". (doi: https://doi.org/10.1101/2024.06.11.598306; https://github.com/mwess/miit)</p> <p>Dataset description:<br>- 9 serial histology sections with the following stains: (HES, HE, HES, HES, HES, MTS, IHC, IHC, HES)<br>- Sections are indexed in the following way (due to some sections not being part of this project): 1,2,3,6,7,8,9,10,11<br>- Each serial section contains: <br> - landmarks with matching labels across all sections.<br> - semi-manually generated tissue masks <br>- Section 2 contain spatial transcriptomics data and one annotation file in geojson format.<br>- Sections 6 and 7 contain imzml data that were generated with MALDI-MSI in positive ion mode (section 6) and negative ion mode (section 7) and additional histology annotations.<br>- MALDI-MSI is reduced. The positive ion data contains only intensities and spectra for spermine. The negative ion mode data contains only intensities and spectra for citrate and zinc.<br>- ST data contains only locations of spots and scalefactors. (I.e. no count data is included.). Barcode ids are randomly generated. <br>- In addition, for each ST spot histopathological annotations and GSEA scores for the Citrate-Spermine Secretion gene signature are provided.</p> <p>Abbreviations:</p> <p>- HES = Hematoxylin-Erythrosine-Saffron<br>- HE = Hematoxylin-Eosin<br>- MTS = Masson's Trichrome Staining<br>- IHC = Immunohistochemistry<br>- ST = Spatial Transcriptomics, here refers to Visium10X arrays.<br>- MALDI-MSI = Matrix-Assisted Laser Desorption Ionization - Mass Spectrometry Imaging.</p> <p> </p>
Images of the work entitled "The spatial distribution of rhizosphere microbial activities under drought: water availability is more important than root-hair controlled exudation"
<p>These images are the images of zymography, <sup>14</sup>C imaging and neutron radiography of the work entitled "The spatial distribution of rhizosphere microbial activities under drought: water availability is more important than root-hair controlled exudation". Raw data on optimal water conditions were partially overlapping with the data of Bilyera et al., 2021, Soil Biology and Biochemistry, 162, 108426.</p>
Protocol for Calcium Imaging and Analysis of Hippocampal CA1 Activity Evoked by Non-Spatial Stimuli
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Technology comparison (image-based spatial transcriptomics)- annotated datasets
<p>This repository contains all the AnnData datasets, regionally annotated, used in the comparison of image-based spatial transcriptomics technologies (Marco Salas et al. 2024)</p>
Multimodal contrastive learning for spatial gene expression prediction using histology images
<p>we employed two human breast cancer datasets and one human cutaneous squamous cell carcinoma (cSCC) dataset.</p>
Imaging Mass Cytometry of human normal colon mucosa (CLN1-6) from: A SIMPLI (Single-cell Identification from MultiPLexed Images) approach for spatially resolved tissue phenotyping at single-cell resolution.
<p>Four µm-thick sections were cut from each block of samples CLN1-CLN6 with a microtome and used for staining with a panel of 26 antibodies targeting the main immune, stromal and epithelial cell populations of the gastrointestinal tract (Supplementary Table 2). The optimal dilution of each antibody in the panel was identified by staining and ablating FFPE appendix sections. The resulting images were reviewed by a mucosal immunologist (J.S.) and the dilution giving the best signal to background ratio was selected for each antibody (Supplementary Table 2). To perform the staining for IMC, slides were dewaxed after a one-hour incubation at 60°C, rehydrated and heat-induced antigen retrieval was performed with a pressure cooker in Antigen Retrieval Reagent-Basic (R&D Systems). Slides were incubated in a 10% BSA (Sigma), 0.1% Tween (Sigma), and 2% Kiovig (Shire Pharmaceuticals) Superblock Blocking Buffer (Thermo Fisher) blocking solution at room temperature for two hours. Each antibody was added to a primary antibody mix at the selected concentration in blocking solution and incubated overnight at 4°C. After two washes in PBS and PBS-0.1% Tween, the slides were treated with the DNA intercalator Cell-ID™ Intercalator-Ir (Fluidigm) (containing the two iridium isotopes 191Ir and 193Ir) 1.25 mM in a PBS solution. After a 30-minute incubation, the slides were washed once in PBS and once in MilliQ water and air-dried. The stained slides were then loaded in the Hyperion Imaging System (Fluidigm) imaging module to obtain light-contrast high resolution images of approximately four mm<sup>2</sup>. These images were used to select the ROI in each slide. For CLN1-CLN6, 1 mm<sup>2 </sup>ROIs were selected to contain the full thickness of the colon mucosa, with epithelial crypts in longitudinal orientation. ROIs were ablated at a o µm/pixel resolution and 200 Hz frequency.</p> <p>Twenty-eight images from 26 antibodies (Supplementary Table 2) and two DNA intercalators were obtained from the raw .txt files of the ablated regions in CLN1-CLN6 using the data extraction process.</p>
Processing steps to generate a Digital Surface Model based on SPOT-7 tri-stereo images published in the study "An assessment of the effects of DEM quality and spatial resolution on a model for mapping lahar inundation areas at volcan Copahue (Argentina & Chile)" in the Journal of South American Earth Sciences https://doi.org/10.1016/j.jsames.2022.104138
<p>The Digital Surface Model (DSM) was created from SPOT-7 tri-stereo images for the Copahue volcano between the border of Argentina and Chile. Two versions of the DSM are provided: an unfiltered product and a final, filtered product. The final product has a spatial resolution of 5-m and was used for lahar inundation modeling for the Copahue volcano (Viotto, Toyos, and Bookhagen 2022, <a href="https://doi.org/10.1016/j.jsames.2022.104138">https://doi.org/10.1016/j.jsames.2022.104138</a> : An assessment of the effects of DEM quality and spatial resolution on a model for mapping lahar hazard inundation at Volcán Copahue (Argentina & Chile). <em>Journal of South American Earth Sciences</em> ). The dataset provided should be cited together with the article. </p> <p><strong>DSM processing </strong></p> <p>The source images were given by a SPOT-7 snow- and cloud-free triplet (Nadir, Backward and Forward) of 1.5 m spatial resolution from 19 April 2018 (SPOT Image, Airbus Defence and Space GmbH, distributed by CONAE; Dataset ID: <em>SEN_SPOT7_20180419_142955500_000</em>, delivered by CONAE as <em>DS_SPOT7_20180419</em>).</p> <p>The data were processed with the suite of digital photogrammetry tools AMES Stereo Pipeline ASP (Beyer et al., 2018). The procedure for the generation of the DSM is summarized by following steps: </p> <ol> <li> <p>The orbital parameters (RCP models) were adjusted using the bundle adjustment tool with no ground control points, since they were unavailable.</p> </li> <li> <p>The scenes were map-projected onto the NASADEM (spatial resolution of 30 m) elevation dataset, assisted by the results of the orbital adjustment in Step 1.</p> </li> <li>The stereo correlation of the map-projected scenes including the results of the adjusted orbital parameters, was performed three times, using as first scene (i.e., primary image) the nadir (N), backward (B), and forward (F) images . In each run, the order of images to perform the stereo correlation was: N-F-B, F-N-B, and B-N-F. Thus, three point clouds were generated. Specific ASP correlator settings (other than defaults parameters; for details see the provided stereo-default file) were set in the following way: <em>Correlation Kernel</em>: 15 x 15 pixels; <em>Sub-pixel Refinement Kernel</em>: 21 x 21 pixels; <em>Subpixel Refinement Mode</em>: 2 (Weighted Affine Adaptive Window Correlator EM)</li> <li> <p>The three point clouds were merged into one point cloud with a regular grid of 5 m (unfiltered product, known as <em>DSM_Copahue_UTM19S_WGS84_5m_raw.tif</em>).</p> </li> </ol> <p>The quality of the final point cloud was assessed by comparing the unfiltered DSM with a spatial resolution of 12-m against the WorldDEM<sup>TM</sup> elevation dataset (Collins et al., 2015). The WorldDEM was provided by Airbus Defence and Space GmbH under license for the scope of the Viotto et al., 2022 study. The comparison of the pixel-to-pixel heights above the ellipsoid (WGS84) between the two datasets resulted in a mean difference of 0.67 m and a standard deviation of +/- 4.82 m. </p> <p>Comprehensive details on the methodologies evaluated to create the dataset with ASP, can be found in the corresponding master's thesis “Topografía digital y modelado de lahares en el Volcán Copahue, Argentina-Chile” from S. Viotto (link: https://rdu.unc.edu.ar/handle/11086/15384). Recommended literature about processing DEMs from SPOT imagery is given by Mueting et al., 2021 (<a href="https://agupubs.onlinelibrary.wiley.com/doi/10.1029/2021JF006330">https://agupubs.onlinelibrary.wiley.com/doi/10.1029/2021JF006330</a>). </p> <p><strong>Creation of the Final, Filtered DSM product</strong></p> <p>The corrections and improvements applied to the unfiltered product to create the final, filtered DSM (named DSM_Copahue_UTM19S_WGS84_5m_VoidFilled.tif) are summarized by following steps. </p> <p> </p> <ol> <li> <p><em>Water Bodies Delineation</em></p> </li> </ol> <p>The delineation of the water bodies was based on a mask created from the free access water bodies datasets provided by the Instituto Geográfico Nacional of Argentina (<a href="https://www.ign.gob.ar/NuestrasActividades/InformacionGeoespacial/CapasSIG">https://www.ign.gob.ar/ NuestrasActividades/InformacionGeoespacia l/CapasSIG</a>) and by the Ministerio de Bienes Nacionales in Chile ( <a href="https://www.ide.cl/index.php/aguas-continentales/item/1508-catastro-de-lagos">https://www.ide.cl/index.php /aguas-continentales/item/1508-catastro-de-lagos</a>). A total of 45 lakes within the area of interest were considered. Lakes with areas below or equal to 25 m2 were smoothed with a median filter in the last step. Lakes with areas above this threshold were filled in with a constant value and their borders were smoothed with a median filter to provide smooth shorelines.</p> <p><em>2 . Void Filling</em></p> <p>Voids (other than water bodies) were filled with the tool “Close Gaps” from Saga GIS software. </p> <p><em>3. Smoothing</em></p> <p>Finally, the elevation dataset was smoothed with a median filter using a 3 x 3 pixel window, excluding water bodies filled in the step 1. </p> <p><strong>Final Remarks and Suggestion</strong></p> <p>The quality assessment of the final version by visual inspection of the hillshades suggested an improvement of the signal to noise ratio. However, the void filling process may be improved.</p> <p><br> </p> <p><strong>Dataset Description</strong></p> <table align="center"> <caption> </caption> <tbody> <tr> <td>Digital Surface Models</td> <td> <p>No Data Value = -9999</p> <p>Format = float 32 bit</p> <p>File Format = GeoTiff</p> <p>Vertical Datum: WGS84</p> <p>Projection information: EPSG 32719 (UTM19S)</p> <p>Spatial Resolution: 5m (subfix: <em>_5m</em>) </p> <p>Versions: </p> <ul> <li> <p>Unfiltered product: without corrections <em>DSM_Copahue_UTM19S_WGS84_5m_raw.tif</em></p> </li> <li> <p>Final, filtered product: smoothed and void filled <em>DSM_Copahue_UTM19S_WGS84_5m_VoidFilled.tif</em></p> </li> </ul> </td> </tr> <tr> <td>Water Bodies Mask</td> <td> <p>No Lake Value = 0</p> <p>Lakes Values = 1 to 45</p> <p>File Format= GeoTiff</p> <p>Spatial Resolution: 5m (subfix: <em>_5m</em>)</p> <p>Projection information : EPSG 32719 (UTM19S)</p> <p><em>WB_mask_5m_UTM19S.tif</em></p> </td> </tr> </tbody> </table> <p> </p> <p> </p> <p><strong>Repository structure</strong></p> <p>|__ 01_Scripts</p> <p> |+ run21_CopahueDSM_AMES_sviotto.sh</p> <p> |+ stereo.default</p> <p>|__ 02_DSMs</p> <p> |+ DSM_Copahue_UTM19S_WGS84_5m_raw.tif</p> <p> |+ DSM_Copahue_UTM19S_WGS84_5m_VoidFilled.tif</p> <p> |+ WB_mask_5m_UTM19S.tif</p> <p><strong>References</strong></p> <p>Beyer, R. A., Alexandrov, O., & McMichael, S. (2018). The Ames Stereo Pipeline: NASA's open source software for deriving and processing terrain data. <em>Earth and Space Science</em>, 5, 537– 548. <a href="https://doi.org/10.1029/2018EA000409">https://doi.org/10.1029/2018EA000409</a></p> <p>Collins, J., Riegler, G., Schrader, H., Tinz, M., 2015. Applying terrain and hydrological editing to TanDEM-X data to create a consumer-ready worlddem product. Int. Arch. Photogram. Rem. Sens. Spatial Inf. Sci. 40 (7), 1149. https://doi.org/10.5194/isprsarchives-XL-7-W3-1149-2015.</p> <p>Mueting, A., Bookhagen, B., & Strecker, M. R. (2021). Identification of debris-flow channels using high-resolution topographic data: A case study in the Quebrada del Toro, NW Argentina. <em>Journal of Geophysical Research: Earth Surface</em>, 126, e2021JF006330. <a href="https://doi.org/10.1029/2021JF006330">https://doi.org/10.1029/2021JF006330</a></p> <p>Viotto, S., Toyos, G., & Bookhagen, B. (2022). An assessment of the effects of DEM quality and spatial resolution on a model for mapping lahar hazard inundation at volcán copahue (Argentina & Chile). Journal of South American Earth Sciences, 104138. https://doi.org/10.1016/j.jsames.2022.104138</p> <p> </p> <p> </p>
Spatialized metabolomic annotation combining MALDI imaging and molecular network
<p>These data are linked to a publication "Spatialized metabolomic annotation combining MALDI imaging and molecular network" where we studied the in situ chemical diversity of fruits of the species Sextonia rubra (Mez.) Van der Werff (Lauraceae) using mass spectrometry imaging techniques and the annotation of molecular species detected by molecular networks using MetGem software. This repository contains: MS1 and MS2 raw data, ion mapping of the fruit according to different tissues, total molecular networks and a script for processing the acquired data to reproduce this approach.<br> <br> </p>
Fig. 2 in Visualizing the spatial distribution of metabolites in Clausena lansium (Lour.) skeels using matrix-assisted laser desorption/ionization mass spectrometry imaging
Fig. 2. Comparison of sample pretreatment methods for MALDI-MSI analysis. (A) Intensities of ion peaks corresponding to organic acids, sugars, and alkaloids in the three different sections using airbrush, iMLayer or combined methods for matrix application. Data represent the mean ± SE of intensities of ions at m/z 230.9, 381.0, 264.1 and 367.1 (n = 3), respectively. Photographs of DHB matrix material prepared by different methods: (B) Spray by airbrush, (C) Sublimation by iMLayer, (D) Spray after sublimation. Films and crystals observation were recorded under the light microscope (× 40).
Fig. 5 in Visualizing the spatial distribution of metabolites in Clausena lansium (Lour.) skeels using matrix-assisted laser desorption/ionization mass spectrometry imaging
Fig. 5. Distribution of the main coumarins in diverse tissue parts in the plant of C. lansium. All the MSI were acquired in positive ion mode. The number of pixels in x and y axis was 243 × 248 for the fruit, and 100 × 70 for the stem and 65 × 37 for the leaf parts. The distributions are displayed as heat maps, with the color code between black (low) and red (high). Images were exported from the Shimadzu Imaging software. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 4 in Visualizing the spatial distribution of metabolites in Clausena lansium (Lour.) skeels using matrix-assisted laser desorption/ionization mass spectrometry imaging
Fig. 4. Distribution of the main alkaloids in diverse tissue parts in the plant of C. lansium. All the MSI were acquired in positive ion mode. The number of pixels in x and y axis was 243 × 248 for the fruit, and 100 × 70 for the stem and 65 × 37 for the leaf parts. The distributions are displayed as heat maps, with the color code between black (low) and red (high). Images were exported from the Shimadzu Imaging software. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 1 in Visualizing the spatial distribution of metabolites in Clausena lansium (Lour.) skeels using matrix-assisted laser desorption/ionization mass spectrometry imaging
Fig. 1. Optical images of different tissue sections of Clausena lansium (Lour.) Skeels plants. (A) Fruit cross section, (B) Part of stem cross section, (C) Leaf cross section (magnification at 40x).
Spatial Frequency Domain Imaging (SFD) for Assessment of Diabetic Foot Ulcer Development and Healing
ClinicalTrials.gov study NCT03341559. IPD Sharing: Not stated. Countries: 1. Publications: 1.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.