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85 results for “visual image”
Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors
<p>Elucidating how synaptic molecules such as AMPA receptors mediate neuronal communication and tracking their dynamic expression during behavior is crucial to understand cognition and disease, but current technological barriers preclude large-scale exploration of molecular dynamics in vivo. We have developed a suite of innovative methodologies that break through these barriers: a new knockin mouse line with fluorescently tagged endogenous AMPA receptors, two-photon imaging of hundreds of thousands of labeled synapses in behaving mice, and computer vision-based automatic synapse detection. Using these tools, we can longitudinally track how the strength of populations of synapses changes during behavior. We used this approach to generate an unprecedentedly detailed spatiotemporal map of synapses undergoing changes in strength following sensory experience. More generally, these tools can be used as an optical probe capable of measuring functional synapse strength across entire brain areas during any behavioral paradigm, describing complex system-wide changes with molecular precision.</p>
Calcium imaging in visual cortex during fidget behaviors in mice
<p><span>Multiple recent studies have shown that motor activity greatly impacts the activity of primary sensory areas like V1. Yet, the role of this motor related activity in sensory processing is still unclear. </span></p> <p><span>Here we dissect how these behavior signals are broadcast to different layers and areas of the visual cortex. To do so, we leveraged a standardized and spontaneous behavioral fidget event in passively viewing mice. Importantly, this behavior event had no relevance to any ongoing task allowing us to compare its neuronal correlates with visually relevant behaviors (e.g running). </span></p> <p><span>A large two-photon Ca</span><span><span>2+</span></span><span> imaging database of neuronal responses uncovered four neural response types during fidgets that were consistent in their proportion and response patterns across all visual areas and layers of the visual cortex. Indeed, the layer and area identity could not be decoded above chance level based only on neuronal recordings. In contrast to running behavior, fidget evoked neural responses that were independent to visual processing. </span></p> <p><span>The broad availability of visually orthogonal standardized behavior signals could be a key component in how the cortex selects, learns and binds local sensory information with motor outputs. Contrary to behaviorally relevant motor outputs, irrelevant motor signals could project to separate local neural subspaces.</span></p> <p><span></span></p>
Tocchini and Mango_An adapted MS2-MCP system to visualize endogenous cytoplasmic mRNA with live imaging in Caenorhabditis elegans_Table S1_Raw data
<p><strong>Table S1. Raw data.</strong> List of quantitation of GFP intensities of heads (<em>spc-1</em>) or pharynges (<em>dlg-1</em>) in the different figures.</p>
Fig. 2 in Visualizing the spatial distribution of metabolites in Clausena lansium (Lour.) skeels using matrix-assisted laser desorption/ionization mass spectrometry imaging
Fig. 2. Comparison of sample pretreatment methods for MALDI-MSI analysis. (A) Intensities of ion peaks corresponding to organic acids, sugars, and alkaloids in the three different sections using airbrush, iMLayer or combined methods for matrix application. Data represent the mean ± SE of intensities of ions at m/z 230.9, 381.0, 264.1 and 367.1 (n = 3), respectively. Photographs of DHB matrix material prepared by different methods: (B) Spray by airbrush, (C) Sublimation by iMLayer, (D) Spray after sublimation. Films and crystals observation were recorded under the light microscope (× 40).
Fig. 5 in Visualizing the spatial distribution of metabolites in Clausena lansium (Lour.) skeels using matrix-assisted laser desorption/ionization mass spectrometry imaging
Fig. 5. Distribution of the main coumarins in diverse tissue parts in the plant of C. lansium. All the MSI were acquired in positive ion mode. The number of pixels in x and y axis was 243 × 248 for the fruit, and 100 × 70 for the stem and 65 × 37 for the leaf parts. The distributions are displayed as heat maps, with the color code between black (low) and red (high). Images were exported from the Shimadzu Imaging software. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 4 in Visualizing the spatial distribution of metabolites in Clausena lansium (Lour.) skeels using matrix-assisted laser desorption/ionization mass spectrometry imaging
Fig. 4. Distribution of the main alkaloids in diverse tissue parts in the plant of C. lansium. All the MSI were acquired in positive ion mode. The number of pixels in x and y axis was 243 × 248 for the fruit, and 100 × 70 for the stem and 65 × 37 for the leaf parts. The distributions are displayed as heat maps, with the color code between black (low) and red (high). Images were exported from the Shimadzu Imaging software. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 1 in Visualizing the spatial distribution of metabolites in Clausena lansium (Lour.) skeels using matrix-assisted laser desorption/ionization mass spectrometry imaging
Fig. 1. Optical images of different tissue sections of Clausena lansium (Lour.) Skeels plants. (A) Fruit cross section, (B) Part of stem cross section, (C) Leaf cross section (magnification at 40x).
High-Resolution PET-CT Imaging for Surgical Margin Visualization
ClinicalTrials.gov study NCT06915454. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Water stages in a tidal marsh measured using images, visually and automatically, along with stages measured using pressure transducer and Doppler sensors
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Estimating density of mountain hares using distance sampling: a comparison of daylight visual surveys, night-time thermal imaging and camera traps
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Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors
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Calcium imaging in visual cortex during fidget behaviors in mice
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Multi-scale optical coherence tomography imaging and visualization of Vermeer's Girl with a Pearl Earring
<p>The data sets, software, and figures accompany the publication:</p> <p>"Multi-scale optical coherence tomography imaging and visualization of Vermeer's Girl with a Pearl Earring", Optics Express 28, 26239 (2020) <a href="https://doi.org/10.1364/OE.390703">https://doi.org/10.1364/OE.390703</a></p> <ul> <li>Fig4_largescaleOCT.pdf, high resolution image of figure 4 of the manuscript</li> <li>Fig5_mediumscaleOCT.pdf, high resolution image of figure 5 of the manuscript</li> <li>Fig6_smallscaleOCT.pdf, high resolution image of figure 6 of the manuscript</li> <li>GWPE_DEMO_V5.zip<br> Zip file of a Windows executable of the virtual rendering of the Girl with the Pearl Earring. The readme file describes the operation of the virtual rendering.</li> <li>Stitching_README.txt Describes the stitching software and the accompanying data sets</li> <li>MS-OCT surface data: <ul> <li>SurfaceStitchedKwadrant11.npy</li> <li>SurfaceStitchedKwadrant12.npy</li> <li>SurfaceStitchedKwadrant21.npy</li> <li>SurfaceStitchedKwadrant22.npy</li> <li>UpperInterfaceMatrixRawdataKwadrant11.npy</li> </ul> </li> <li>MS-OCT thickness data <ul> <li>FullResGlazeStratigraphyThicknessKwadrant11.npy</li> <li>FullResGlazeStratigraphyThicknessKwadrant12.npy</li> <li>FullResGlazeStratigraphyThicknessKwadrant21.npy</li> <li>FullResGlazeStratigraphyThicknessKwadrant22.npy</li> </ul> </li> <li>MS-OCT Scattering strength data <ul> <li>FullResScatteringStrengthKwadrant11.npy</li> <li>FullResScatteringStrengthKwadrant12.npy</li> <li>FullResScatteringStrengthKwadrant21.npy</li> </ul> </li> <li>Main.py, DataAnalysisFunctionsRepository.py, and ReadAndCombineKwadrantData.py are Python files for stitching the four quadrants of data together. Runs in Python >3.5. Full stitching image takes more than an hourFullResScatteringStrengthKwadrant22.npy</li> <li>Plot_measure.py and plot_fit.py<br> Analysis of the BRDF measurements of the black underlayer background (RU1) and glaze layer (RU5). Runs in Python > 3.5</li> <li>Visualization1.ave<br> Video of the multi-scale multi-parameter OCT data set of the Girl</li> <li>Visualization2.mp4<br> Video showing an impression of the interactive demo of the Girl <ul> </ul> </li> </ul>
SEM Data Base Microvilli Semantic Segmentation in Microscopic Images Using a Visual Learning Pipeline
<p>SEM images raw data X1, X1, Y1, Y2, Z1 and Z2</p>
Fig. 3 in Visualizing the spatial distribution of metabolites in Clausena lansium (Lour.) skeels using matrix-assisted laser desorption/ionization mass spectrometry imaging
Fig. 3. MALDI-MS spectrum of C. lansium fruit extract in positive ion mode.
A Study Assessing the Safety and Effectiveness of IC2000 and SPY Fluorescence Imaging Systems (SPY-PHI) in the Visualization of Lymphatic Vessels and Identification of Lymph Nodes During Sentinel Lymp
ClinicalTrials.gov study NCT03200704. IPD Sharing: Not stated. Countries: 2. Publications: 0.
Realistic in Generation of HEp-2 Cell Images Using Latent Diffusion Models: a Multi-center Visual Turing Test
ClinicalTrials.gov study NCT06542783. IPD Sharing: NO. Countries: 0. Publications: 6.
Use of Transcranial Magnetic Stimulation (TMS) and Magnetic Resonance Imaging (MRI) to Study Visual Attention
ClinicalTrials.gov study NCT00006285. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Patient Specific Virtual Reality for Simulation of Spine Procedures: an Intelligent Image Segmentation, Registration and 3-dimensional Visualization in a Unified Virtual Reality Workflow for Image Gui
ClinicalTrials.gov study NCT06714539. IPD Sharing: UNDECIDED. Countries: 0. Publications: 1.
Compare Rates of Agreement Between Clinical Diagnosis and Visual Assessment of DaTscan™ Images in Non-Caucasian and Caucasian Subjects With Parkinson's Disease (PD) or Essential Tremor (ET)
ClinicalTrials.gov study NCT01952678. IPD Sharing: Not stated. Countries: 1. Publications: 0.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.