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48 results for “voucher specimens”
FIGURES 3, 4—Green Hong Kong A in The Hemiptera-Sternorrhyncha (Insecta) of Hong Kong, China-an annotated inventory citing voucher specimens and published records
FIGURES 3, 4—Green Hong Kong A. (3) A spillway from the Aberdeen reservoirs on HK Island, flanked by slopes covered by diverse woodland. (4) Country Park walking trails miander for miles throughout HK's territory, affording ready access for insect sampling.
FIGURES 1, 2 in The Hemiptera-Sternorrhyncha (Insecta) of Hong Kong, China-an annotated inventory citing voucher specimens and published records
FIGURES 1, 2— Developed Hong Kong. (1) The coastal fringe of HK Island has long been a byword for population density. (2) The new town of Tung Chung on the developing north side of Lantau Island, with appartment towers, expressway and rapid transit links.
Data from: Voucher specimens of Petrocosmea wangii sp. nov. and P. yei sp. nov.
<p>In 2020, we conducted fieldwork in some limestone areas where there were no previous records of <em>Petrocosmea</em>. On August 5th, a small population of an unknown <em>Petrocosmea</em> species was discovered on the stone wall of a small sinkhole in a limestone valley occupied by <em>Pinus yunnanensis</em> Franch. in the northern part of Shiping County, Yunnan. Morphologically, the plant closely resembles <em>P. sericea</em>, but with an extremely short corolla tube (only 2 mm, the shortest in all known species of<em> Petrocosmea</em>) and glabrous and straight filaments. We describe this species as<em> P. wangii</em>. On September 19th, another unknown species of <em>Petrocosmea </em>was collected in Mojiang County. The individuals we planted in the greenhouse in December of that year produced flowers similar to <em>P. forrestii</em>, and we subsequently went to the same locality during flowering to confirm the prevalence of the flower morphology. Morphologically, it most closely resembles<em> P. forrestii</em>, but its filaments are adnate to the base of the corolla tube for about 2 mm, which easily differentiate it from all other known species of <em>Petrocosmea</em>, and we describe this species as <em>P. yei</em>.</p>
FIGURES 5–8. Type specimen and molecular voucher images. 5 in A New Monotypic Genus from the American Southwest to accommodate "Semiothisa" kuschea (Geometridae: Ennominae)
FIGURES 5–8. Type specimen and molecular voucher images. 5, HOLOTYPE male of Semiothisa kuschea, and 6, label data (image credit Chris Grinter); 7, voucher image of "Isochromodes" "infida" (GB-CR-1241), sister taxon of Metrica misidentified as Certima dositheata in Murillo-Ramos et al. (2019) and Brehm et al. (2019) (image taken from, and image credit to, Supplement File 5 of Brehm et al. (2019)); 8, SYNTYPES of Sabulodes infida from USNM type search (https://collections.USNM.si.edu/ search/ento/). Images not to scale.
FIGURE. Phylogenetic tree of specimens on Poaceae and related host plants constructed by MP method based on ITS+28S regions of rDNA. Bootstrap values of MP and ML are followed by the Bayesian posterior probabilities (Bpp) on the nodes in the topology. Asterisk (*) represents bootstrap values or Bpp less than 50% in the topology. Sample data are shown with voucher specimen number or GenBank accession number, and host plant. Sequence data determined in this study are shown in color. Teliospore shapes are shown in each clade detected, and new species are shown by asterisk (*) on clades. 0, I: Spermogonial and aecial host genus. Asterisk (*) on host plants: Spermogonial and aecial host plants. in Phylogenetic approach for identification and life cycles of Puccinia (Pucciniaceae) species on Poaceae from northeastern China
FIGURE. Phylogenetic tree of specimens on Poaceae and related host plants constructed by MP method based on ITS+28S regions of rDNA. Bootstrap values of MP and ML are followed by the Bayesian posterior probabilities (Bpp) on the nodes in the topology. Asterisk (*) represents bootstrap values or Bpp less than 50% in the topology. Sample data are shown with voucher specimen number or GenBank accession number, and host plant. Sequence data determined in this study are shown in color. Teliospore shapes are shown in each clade detected, and new species are shown by asterisk (*) on clades. 0, I: Spermogonial and aecial host genus. Asterisk (*) on host plants: Spermogonial and aecial host plants.
Integrative biodiversity inventories: characterizing lichen-forming fungal diversity in Glen Canyon National Recreation Area using DNA barcoding and vouchered specimens
<p>The Colorado River and its tributaries on the Colorado Plateau are home to unique desert river ecosystems and changing environmental conditions. Within this region, the Glen Canyon National Recreation Area (GCNRA) is comprised of rugged, high desert terrain and is managed by the United States National Parks Service as both a recreational and conservation area. Despite the ecological and economic importance of GCNRA, significant components of the ecological communities therein remain poorly characterized, including lichens. Accurately characterizing lichen-forming fungal diversity is challenging due to poorly known taxonomic groups, underexplored regions/habitats, and varying interpretations of morphological differences, including the recognition of environmentally modified forms. To better understand lichen diversity in GCNRA, we used an integrative taxonomic approach, incorporating both traditional morphology-based identification and information from the standard fungal DNA barcoding marker, the ITS, to compile a thorough inventory of lichen-forming fungi in Fifty-Mile Canyon. Vouchered lichen specimens were collected in 2019, and from these the ITS marker was sequenced. Candidate species-level lineages were delimited from family-level multiple sequence alignments using the Assemble Species by Automatic Partitioning web server. Specimens comprising DNA-based candidate species were then evaluated using traditional taxonomically diagnostic characters to link these, where possible, to currently described species. For Fifty-Mile Canyon, we document 100 putative species in 15 families, each represented by vouchered specimens, ITS sequence data, and photographic documentation. For comparison, a survey of historic records from GCNRA revealed a total of 124 documented lichen-forming fungal species throughout the NRA and adjacent land. Approximately 50% of the species documented in Fifty-Mile Canyon had not previously been found in GCNRA, and similar proportions of species diversity have been documented in GCNRA but not observed in our survey. We report three species new to North America – <em>Calogaya ferrugineoides</em> (H. Magn.) Arup, Froden & Sochting, <em>Endocarpon deserticola</em> T. Zhang, X. L. Wei & J. C. Wei and <em>Xanthocarpia ferrari</em> (Bagl.) Frödén, Arup & Søchting – verified using ITS sequencing data. In addition, <em>Circinaria squamulosa</em> sp. nov. is formally described here, currently known only from sandstone slabs in Fifty-Mile Canyon. However, the taxonomic identity of many of the candidate species from Fifty-Mile Canyon remained ambiguous at the species level, and some collections likely represent undescribed species-level lineages. Our results revealed unexpected, high species-level diversity of lichen-forming fungi at local scales and that overall lichen diversity across the entire GCNRA is likely vastly undercounted. These data – including DNA barcodes for the vast majority of lichen-forming fungi occurring in this canyon – provide an important resource that can be integrated into subsequent lichen biodiversity research in the southwestern United States and other semi-arid climates.</p>
Supplementary material 1 from: Gutiérrez EE, Helgen KM, McDonough MM, Bauer F, Hawkins MTR, Escobedo-Morales LA, Patterson BD, Maldonado JE (2017) A gene-tree test of the traditional taxonomy of American deer: the importance of voucher specimens, geographic data, and dense sampling. ZooKeys 697: 87-131. https://doi.org/10.3897/zookeys.697.15124
Gazetteer : Authors: Eliécer E. Gutiérrez, Kristofer M. Helgen, Molly M. McDonough, Franziska Bauer, Melissa T. R. Hawkins, Luis A. Escobedo-Morales, Bruce D. Patterson, Jesús E. Maldonado
Supplementary material 3 from: Gutiérrez EE, Helgen KM, McDonough MM, Bauer F, Hawkins MTR, Escobedo-Morales LA, Patterson BD, Maldonado JE (2017) A gene-tree test of the traditional taxonomy of American deer: the importance of voucher specimens, geographic data, and dense sampling. ZooKeys 697: 87-131. https://doi.org/10.3897/zookeys.697.15124
Supplementary information figure : Authors: Eliécer E. Gutiérrez, Kristofer M. Helgen, Molly M. McDonough, Franziska Bauer, Melissa T. R. Hawkins, Luis A. Escobedo-Morales, Bruce D. Patterson, Jesús E. Maldonado
Supplementary material 2 from: Gutiérrez EE, Helgen KM, McDonough MM, Bauer F, Hawkins MTR, Escobedo-Morales LA, Patterson BD, Maldonado JE (2017) A gene-tree test of the traditional taxonomy of American deer: the importance of voucher specimens, geographic data, and dense sampling. ZooKeys 697: 87-131. https://doi.org/10.3897/zookeys.697.15124
Name and DNA sequences of pairs of primers used for amplification and sequencing of the CYTB gene : Data type: molecular data
Subspecies and Distribution. M.l.leucogasterMilne-Edwards,1872—C&EChinaandNVietnam. M. l. rubex Thomas, 1916 — NE India (West Bengal); a record from Nepal is not supported by any voucher specimens and is not mapped here. in Vespertilionidae
Subspecies and Distribution. M.l.leucogasterMilne-Edwards,1872—C&EChinaandNVietnam. M. l. rubex Thomas, 1916 — NE India (West Bengal); a record from Nepal is not supported by any voucher specimens and is not mapped here.
Subspecies and Distribution. P.m.macrotisPeters,1867—AruIs(Indo-nesia). P. m. epularius Ramsay, 1878 — New Guinea and Raja Ampat Is (Salawati). Also sightings and photographs in Australian Boigu Is, but voucher specimens are needed for confirmation. in Pteropodidae
Subspecies and Distribution. P.m.macrotisPeters,1867—AruIs(Indo-nesia). P. m. epularius Ramsay, 1878 — New Guinea and Raja Ampat Is (Salawati). Also sightings and photographs in Australian Boigu Is, but voucher specimens are needed for confirmation.
Distribution. Known from Gansu, Shaanxi, Sichuan, and Yunnan, China; distribution should be treated with caution because of its similarity with S. bedfordiae. Only voucher specimens from N & W Sichuan have been examined. in Soricidae
Distribution. Known from Gansu, Shaanxi, Sichuan, and Yunnan, China; distribution should be treated with caution because of its similarity with S. bedfordiae. Only voucher specimens from N & W Sichuan have been examined.
FIGURES 8–9. Voucher specimens. 8, Dugdaleiella monticola male. 8a in New species of Dugdaleiella, gen. nov., Kozloviella, gen. nov., and Pfitzneriella Viette from upper elevation Andes of Ecuador and Peru (Lepidoptera: Hepialidae)
FIGURES 8–9. Voucher specimens. 8, Dugdaleiella monticola male. 8a, LT (ZMHB); 8b, Cotopaxi, Ecuador (CMNH); 8c, Volcán Chiles, Ecuador (CMNH); 8d, male, Volcán Chiles, Ecuador (CMNH). 9, Labial palpi (male unless otherwise specified), 9a, Kozloviella viazmenskyi sp. n.; 9b, Pfitzneriella antonkozlovi sp. n.; 9c, P. rawlinsi sp. n.; 9d, P. titarenkoi sp. n.; 9e, P. yuliyakovalevae sp. n.; 9f, P. yuliyakovalevae sp. n. (female); 9g, Dugdaleiella monticola. Photos: Jane Hyland (Fig. 8), John Grehan (Figs 9a–b, d–h), and Carlos Mielke (Fig. 9c). Scale 1 mm (when present).
FIGURES 1–7. Voucher specimens. 1, Kozloviella viazmenskyi gen. n in New species of Dugdaleiella, gen. nov., Kozloviella, gen. nov., and Pfitzneriella Viette from upper elevation Andes of Ecuador and Peru (Lepidoptera: Hepialidae)
FIGURES 1–7. Voucher specimens. 1, Kozloviella viazmenskyi gen. n., sp. n. male HT (CMNH). 1a, dorsal; 1b, ventral. 2, Pfitzneriella antonkozlovi sp. n. male HT (CMNH), 2a, dorsal; 2b, ventral. 3, P. olafi sp. n. male HT (MNHN), 3a, dorsal; 3b, ventral. 4, P. rawlinsi sp. n. male, 4a, HT dorsal (CMNH); 4b, PT dorsal; 4c, PT ventral (CGCM). 5, P. remota male HT dorsal (MNHN). 6, P. titarenkoi sp. n. male HT (CMNH), 6a, dorsal; 6b, ventral. 7, P. yuliyakovalevae sp. n. male HT (CMNH), 7a, dorsal; 7b, ventral. Female PT (CMNH); 7c, dorsal; 7d, ventral. Photos: Ernst Brockmann (Fig. 5), Jane Hyland (Fig. 4a), Anton Kozlov (Figs 1, 2, 6, 7), and Carlos Mielke (Figs 3, 4b, 4c).
FIGURE 2 in An example of problems associated with DNA barcoding in tardigrades: a novel method for obtaining voucher specimens
FIGURE 2. Granulated cuticle and pores in a fresh (A, DIC) and an orcein stained (B, PhC) specimen of M. terminalis (C2868); C: Granulated cuticle (arrow head) and pores by SEM in M. terminalis (C2868); D: Smooth cuticle with pores in a fresh specimen of M. macrocalix (C2868, DIC); E: Granulated cuticle (arrow head) in the holotype of M. terminalis (C624- S60, DIC). Bar =10 µm (A, B, D, E); 5 µm (C)
FIGURE 1. A in An example of problems associated with DNA barcoding in tardigrades: a novel method for obtaining voucher specimens
FIGURE 1. A: Fresh specimen of M. terminalis; buccal pharyngeal apparatus with dorsal buccal armature (C2868, DIC); B: Fresh specimen of M. macrocalix; buccal pharyngeal apparatus with dorsal buccal armature (C2868, DIC). C: Claws with indented lunules on a hind leg of M. terminalis (C2868, SEM); D: Claws with indented lunules (arrow heads) on the hind legs of a fresh specimen of M. terminalis (C2868, DIC). Bar =10 µm (A, B, D); 5 µm (C)
FIGURE 5 in An example of problems associated with DNA barcoding in tardigrades: a novel method for obtaining voucher specimens
FIGURE 5. Neighbor joining dendrogram computed on Kimura 2-parameter distances. Numbers in bold indicate bootstrap values. Specimens are indicated with either GenBank accession numbers or with acronyms as in Table 1 (in bold).
FIGURE 3. A in An example of problems associated with DNA barcoding in tardigrades: a novel method for obtaining voucher specimens
FIGURE 3. A: Egg shell of M. terminalis (hologenophore C2868-N02 US2, DIC); B: Egg shell of M. terminalis by SEM (C2868); C: Egg shell of a M. terminalis paratype (C624-S44, PhC); D: Egg shell of M. cf. terminalis (C2341, PhC). Bar =10 µm (A, C, D); 1 µm (B).
Data from: Voucher specimens of Petrocosmea wangii sp. nov. and P. yei sp. nov.
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Integrative biodiversity inventories: characterizing lichen-forming fungal diversity in Glen Canyon National Recreation Area using DNA barcoding and vouchered specimens
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