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34 results for “western blot”
Fig. 6. Western blot analysis with a p in Mutagenesis of a Lotus japonicus GSK3β/Shaggy-like kinase reveals functionally conserved regulatory residues
Fig. 6. Western blot analysis with a p-GSK-3α/β (6D3) (Santa Cruz Biotechnology, Inc.) monoclonal antibody raised against a phosphopeptide corresponding to amino acids residues surrounding Tyr279 of human GSK3α and Tyr216 of human GSK3β. Lane 1: recombinant LjSK1 and lane 2: LjSK1 Y298A variant. Both proteins have a GST-tag.
original images for western blots
<p>original images for western blots</p>
Western Blot and Gel source data
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Western blots of liver-expressed genes in C57BL/NCrl mice gavaged every 4 days for 28 days with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
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Representative Western blots showing the ratio of total expression of phosphorylated and unphosphorylated proteins involved in insulin signaling pathway in the red gastrocnemius muscle in control and HFD groups after chronic CBD treatment.
<p>Representative Western blots showing the ratio of total expression of phosphorylated and unphosphorylated proteins involved in insulin signaling pathway e.g., phosphorylated insulin receptor substrate 1/insulin receptor substrate 1 - pIRS-1/IRS-1 (A), phosphorylated protein kinase B/protein kinase B - pAkt/Akt (B), phosphorylated AS160 protein/AS160 protein – pAS160/AS160 (C) and phosphorylated glycogen synthase kinase 3/glycogen synthase kinase 3 - pGSK-3/GSK-3 (D) in the red gastrocnemius muscle in control and HFD groups after chronic CBD treatment. The total expressions of the above proteins were presented as a percentage difference compared to the control group, which was set as 100%. The data are expressed as mean values ± SD, n = 6 in each group. ap < 0.05 significant difference: control group vs. examined group; bp < 0.05 significant difference: HFD vs. HFD+CBD.</p>
Supplemental Figure S1. Uncropped data for a Western blot analysis
<p>Supplemental Figure S1. Uncropped data for a Western blot analysis</p>
Western blot quantification dataset for Structure of LRRK1 and mechanisms of autoinhibition and activation
<p>Tabular data corresponding to Western blots. </p>
Toward countering muscle and bone loss with spaceflight: GSK3 as a potential target (Tibialis Anterior, RR9, Western Blot)
We examined the effects of ~30 days of spaceflight on glycogen synthase kinase 3 (GSK3) content and inhibitory serine phosphorylation in murine muscle and bone samples from four separate missions (BION-M1, rodent research [RR]1, RR9, and RR18). Spaceflight reduced GSK3b content across all missions, whereas its serine phosphorylation was elevated with RR18 and BION-M1. The reduction in GSK3b was linked to the reduction in type IIA fibers commonly observed with spaceflight as these fibers are particularly enriched with GSK3. We then tested the effects of inhibiting GSK3 before this fiber type shift, and we demonstrate that muscle-specific Gsk3 knockdown increased muscle mass, preserved muscle strength, and promoted the oxidative fiber type with Earth-based hindlimb unloading. In bone, GSK3 activation was enhanced after spaceflight; and strikingly, muscle-specific Gsk3 deletion increased bone mineral density in response to hindlimb unloading. Thus, future studies should test the effects of GSK3 inhibition during spaceflight. This study derives results from the Western blot assay using tibialis anterior tissue from the RR9 mission. The tibialis anterior data in this study are related to other studies using tissues from the same experiment; OSD-654 (tibia), OSD-661 (lumbar spine), OSD-662 (soleus), OSD-663 (femur), and OSD-664 (extensor digitorum longus).
Effects of Space Flight on Inflammasome Activation in the Brain of Mice (Brain, Western Blot and ECLIA)
Space exploration exposes astronauts to stressors that alter the immune response, rendering them vulnerable to infections and diseases. In this study, we aimed to determine the levels of inflammasome activation in the brains of mice that were housed in the International Space Station (ISS) for 37 days. C57BL/6 mice were launched to the ISS as part of NASA’s Rodent Research 1 Mission on SpaceX-4 CRS-4 Dragon cargo spacecraft from September 21st, 2014, to October 25th, 2014. Dissected mouse brains from that mission were analyzed by immunoblotting of inflammasome signaling proteins and Electrochemiluminescence Immunoassay (ECLIA) for inflammatory cytokine levels. Our data indicate decreased inflammasome activation in the brains of mice that were housed in the ISS for 37 days when compared to the brains of mice that were maintained on the ground, and in mice corresponding to the baseline group that were sacrificed at the time of launching of SpaceX-4. Moreover, we did not detect any significant changes in the expression levels of the pro-inflammatory cytokines TNF-alpha, IL-2, IFN-gamma, IL-5, IL-6, IL-12p70 and IL-10 between the ground control and the flight groups. Together, these studies suggest that spaceflight results in a decrease in the levels of innate immune signaling molecules that govern inflammasome signaling in the brain of mice. This dataset derives results from the ECLIA and Western Blot assays using brain tissue.
Effects of low-dose oxygen ions and protons on cardiac function and structure in male C57BL/6J mice (western blot, histology, and immunohistochemistry)
Purpose: Astronauts traveling beyond low Earth orbit will be exposed to high linear energy transfer charged particles. Because there is concern about the adverse effects of space radiation on the cardiovascular system, this study assessed cardiac function and structure and immune cell infiltration in a mouse model of charged particle irradiation. Materials and methods: Male C57BL/6 J mice were exposed to oxygen ions (16O, 600 MeV/n at 0.25 to 0.26 Gy/min to a total dose of 0, 0.05, 0.1, 0.25, or 1 Gy), protons (150 MeV, 0.35 to 0.55 Gy/min to 0, 0.5, or 1 Gy), or protons (150 MeV, 0.5 Gy) followed by 16O (600 MeV/n, 0.1 Gy). Separate groups of mice received 137Cs γ rays (1 Gy/min to 0, 0.5, 1, or 3 Gy) as a reference. Cardiac function and blood velocity were measured with ultrasonography at 3, 5, 7, and 9 months after irradiation. At 2 weeks, 3 months, and 9 months, cardiac tissue was collected to assess apoptosis, tissue remodeling, and markers of immune cells. Results: Ejection fraction and fractional shortening decreased at 3 and 7 months after 16O. These parameters did not change in mice exposed to γ rays, protons, or protons followed by 16O. Each of the radiation exposures caused only small increases in cleaved caspase3 and numbers of apoptotic nuclei. Changes in the levels of α smooth muscle cell actin and a 75kDa peptide of collagen type III in the left ventricle suggested tissue remodeling, but there was no significant change in total collagen deposition at 2 weeks, 3 months, and 9 months. Increases in protein amounts of cluster of differentiation (CD)2, CD68, and CD45 as measured with immunoblots at 2 weeks, 3 months, and 9 months after exposure to protons or 16O alone suggested immune cell infiltration. For type III collagen, CD2 and CD68, the efficacy in inducing protein abundance of CD2, CD68, and CD45 was 16O greater than protons was greater than γ rays greater than protons followed by 16O. Conclusions: Low dose, high energy charged particle irradiation caused mild changes in cardiac function and tissue remodeling in the mouse. This dataset derives result from the western blot, histology, and immunohistochemistry assays.
Characterizing SERCA Function in Murine Skeletal Muscles after 35-37 Days of Spaceflight from RR-1 and RR-9 (calcium uptake and Western blot in soleus and tibialis anterior)
It is well established that microgravity exposure causes significant muscle weakness and atrophy via muscle unloading. On Earth, muscle unloading leads to a disproportionate loss in muscle force and size with the loss in muscle force occurring at a faster rate. Although the exact mechanisms are unknown, a role for Ca2+ dysregulation has been suggested. The sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA) pump actively brings cytosolic Ca2+ into the SR, eliciting muscle relaxation and maintaining low intracellular Ca2+ ([Ca2+]i). SERCA dysfunction contributes to elevations in [Ca2+]i, leading to cellular damage, and may contribute to the muscle weakness and atrophy observed with spaceflight. Here, we investigated SERCA function, SERCA regulatory protein content, and reactive oxygen/nitrogen species (RONS) protein adduction in murine skeletal muscle after 35-37 days of spaceflight. In male and female soleus muscles, spaceflight led to drastic impairments in Ca2+ uptake despite significant increases in SERCA1a protein content. We attribute this impairment to an increase in RONS production and elevated total protein tyrosine (T) nitration and cysteine (S) nitrosylation. Contrarily, in the tibialis anterior (TA), we observed an enhancement in Ca2+ uptake, which we attribute to a shift towards a faster muscle fiber type (i.e., increased myosin heavy chain IIb and SERCA1a) without elevated total protein T-nitration and S-nitrosylation. Thus, spaceflight affects SERCA function differently between the soleus and TA. This dataset derives results from the calcium uptake (spectrofluorometry) and Western blot assays.
Original Western Blot Images_Papers "The PIK3CA H1047R Mutation Confers Resistance to BRAF and MEK Inhibitors in A375 Melanoma Cells through the Cross-Activation of MAPK and PI3K/Akt Pathways"
<p>Original Western Blot Images_Papers "The PIK3CA H1047R Mutation Confers Resistance to BRAF and MEK Inhibitors in A375 Melanoma Cells through the Cross-Activation of MAPK and PI3K/Akt Pathways" which is submitted to Pharmaceutics Journal for publication.</p>
western blots
<p>The western blots of the manuscript "Panax quinquefolium saponin prevents postmenopausal atherosclerosis by inhibiting inflammation and apoptosis via regulation of the ERα/MEK/ERK1/2 and ERα/PI3K/Akt pathways".</p>
Western Blot of Figure 4
<p>Western blot analysis of p-STAT3 (MW=86 kDa) and STAT3 (MW=79 kDa) proteins in Mφ untreated or treated with 10 μM OB 24, 10 μM SnPPIX, 5 μM ZnPPIX and 5 μM CoPPIX. β-actin (MW=42 kDa) was used as a control.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.