Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
472
datasets available to search
ShareScore release 0.9.0
Dataset results
472 results for “whole genome sequencing”
Demogaphic Reconstruction of the Western Sheep Expansion from Whole-Genome sequences
<p>Sheep (<em>Ovis aries</em>) were among the earliest livestock, domesticated in the Fertile Crescent about 12000-10000 years ago with a nearly worldwide distribution today. Most of our knowledge about the timing of their expansion stems from archaeological data but it is unclear how the genetic diversity of modern sheep fits with these dates. We used whole-genome sequencing data of 63 domestic breeds and their wild relatives, the Asiatic mouflon (<em>O. gmelini</em>), to explore the demographic history of sheep. <br> On the global scale, our analysis revealed geographic structuring among breeds with unidirectional recent gene flow from domestics into Asiatic mouflons. We then selected four representative breeds from Spain, Morocco, the UK and Iran to build a comprehensive demographic model of the western sheep expansion.<br> We inferred a single domestication event around 9,000 years ago, slightly later than archaeological evidence suggests which might reflect uncertainties in the generation time used for these estimates. The westward expansion is dated to approximately 5,000 years ago, later than the original Neolithic expansion of sheep and approximately matching the Secondary Product Revolution associated with woolly sheep. We see some signals of recent gene flow from an ancestral population into southern European breeds which could reflect admixture with feral European mouflon. Furthermore, our results indicate that many breeds experienced a reduction of their effective population size during the last centuries, probably associated with the breed development.<br> Our study provides insights into the complex demographic history of western Eurasian sheep, highlighting interactions between breeds and their wild counterparts.</p>
Whole-genome sequences of Cercospora beticola isolates from Germany and Italy.
<p>Sequences used in the manuscript <strong>"Large-scale analyses reveal the contribution of adaptive evolution in pathogenic and non-pathogenic fungal species"</strong></p>
Supplemental material of "An annotated whole-genome multilocus sequence typing schema for scalable high resolution typing of Streptococcus pyogenes"
<p>This supplemental material includes the genome assemblies, associated metadata and analysis results for five datasets used to define a publicly available annotated wgMLST schema for <em>S. pyogenes</em> and to evaluate its suitability for high resolution typing. A brief description for each file in the dataset is available in the included README file. Raw sequencing data and sample metadata for the 265 isolates included in Dataset1 have been deposited in the European Nucleotide Archive (ENA) under Project <a href="https://www.ebi.ac.uk/ena/browser/view/PRJEB49967?show=reads">PRJEB49967</a>.</p> <p>The wgMLST schema was created with <a href="https://github.com/B-UMMI/chewBBACA">chewBBACA</a> and is publicly available at <a href="https://chewbbaca.online/species/1/schemas/1">chewie-NS</a>, where a more detailed description of schema creation, annotation and curation can be found.</p>
Whole genome sequence and annotation dataset of rare actinobacteria, Barrientosiimonas humi gen. nov., sp. nov. 39T from Antarctica
<p>The present data files are the source files of the annotation output from the whole genome sequencing of rare actinobacteria, <em>Barrientosiimonas humi gen. nov., sp. nov.</em> 39<sup>T</sup> from Antarctica.</p> <p>The dataset of the whole-genome sequence of <em>B. humi</em> had been deposited in European Nucleotide Archive (ENA) repository under the accession number PRJEB44986 / ERP129097, direct URL to data:<strong> </strong><a href="https://www.ebi.ac.uk/ena/browser/view/PRJEB44986">https://www.ebi.ac.uk/ena/browser/view/PRJEB44986</a></p>
Integrative QTL mapping and selection signatures in Groningen White Headed cattle inferred from whole-genome sequences
<p>Here, we aimed to identify and characterize genomic regions that differ between Groningen White Headed (GWH) breed and other cattle, and in particular to identify candidate genes associated with coat color and/or eye-protective phenotypes. Firstly, whole genome sequences of 170 animals from eight breeds were used to evaluate the genetic structure of the GWH in relation to other cattle breeds by carrying out principal components and model-based clustering analyses. Secondly, the candidate genomic regions were identified by integrating the findings from: a) a genome-wide association study using GWH, other white headed breeds (Hereford and Simmental), and breeds with a non-white headed phenotype (Dutch Friesian, Deep Red, Meuse-Rhine-Yssel, Dutch Belted, and Holstein Friesian); b) scans for specific signatures of selection in GWH cattle by comparison with four other Dutch traditional breeds (Dutch Friesian, Deep Red, Meuse-Rhine-Yssel and Dutch Belted) and the commercial Holstein Friesian; and c) detection of candidate genes identified via these approaches. The alignment of the filtered reads to the reference genome (ARS-UCD1.2) resulted in a mean depth of coverage of 8.7X. After variant calling, the lowest number of breed-specific variants was detected in Holstein Friesian (148,213), and the largest in Deep Red (558,909). By integrating the results, we identified five genomic regions under selection on BTA4 (70.2–71.3 Mb), BTA5 (10.0–19.7 Mb), BTA20 (10.0–19.9 and 20.0–22.7 Mb), and BTA25 (0.5–9.2 Mb). These regions contain positional and functional candidate genes associated with retinal degeneration (e.g., <em>CWC27</em> and <em>CLUAP1</em>), ultraviole<em>t</em> protection (e.g., <em>ERCC8</em>), and pigmentation (e.g. <em>PDE4D</em>) which are probably associated with the GWH specific pigmentation and/or eye-protective phenotypes, e.g. Ambilateral Circumocular Pigmentation (ACOP). Our results will assist in characterizing the molecular basis of GWH phenotypes and the biological implications of its adaptation.</p>
Whole genome sequence and annotation of Penstemon davidsonii
<p><em><span>Penstemon</span></em><span> is the most speciose flowering plant genus endemic to North America. <em>Penstemon</em> species' diverse morphology and adaptation to various environments have made them a valuable model system for studying evolution, but the absence of publicly available reference genomes limits possible research directions. Here we report the first reference genome assembly and annotation for <em>Penstemon</em> <em>davidsonii</em>. Using PacBio long-read sequencing and Hi-C scaffolding technology, we constructed a de novo reference genome of 437,568,744 bases, with a contig N50 of 40 Mb and L50 of 5. The annotation includes 18,199 gene models, and both the genome and transcriptome assembly contain over 95% complete eudicot BUSCOs. This genome assembly will serve as a valuable reference for studying the evolutionary history and genetic diversity of the <em>Penstemon</em> genus.</span></p>
Whole genome sequence and annotation of Penstemon davidsonii
Open the record for dataset details and reuse information.
Whole genome sequence data of Mycobacterium tuberculosis and Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic pathway- Part 1
Open the record for dataset details and reuse information.
Whole genome sequence data of Mycobacterium tuberculosis and Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic pathway- Part 2
Open the record for dataset details and reuse information.
Whole-genome sequencing reveals asymmetric introgression between two sister species of cold-resistant leaf beetles
Open the record for dataset details and reuse information.
Whole genome sequences of 23 species from the Drosophila montium species group (Diptera: Drosophilidae): a resource for testing evolutionary hypotheses
Open the record for dataset details and reuse information.
ParaMask, a new method to identify multicopy genomic regions, corrects major biases in whole-genome sequencing data. Additional Datasets.
<p>Data supporting the main figures of the "ParaMask, a new method to identify multicopy genomic regions, corrects major biases in whole-genome sequencing data" manuscript and a copy of the ParaMask software and scripts for analysis, and SV calls from longreads. README files are included.</p>
Data required for "Low mutation rate of spontaneous mutants enables detection of causative genes by comparing whole genome sequences"
<p>In the early 1900s,mutation breeding to select varieties with desirable traits using spontaneous mutation was actively conducted around the world, including Japan. In rice, the number of fixed mutations per generation was estimated to be 1.38-2.25. Although this low mutation rate was a major problem for breeding in those days, in the modern era with the development of NGS technology, it was conversely considered to be an advantage for efficient gene identification. In this paper, we proposed an in silico approach using next-generation sequencing (NGS) to compare the whole genome sequence of a spontaneous mutant with that of a closely related strain with a nearly identical genome, to find polymorphisms that differ between them, and to identify the causal gene by predicting the functional variation of the gene caused by the polymorphism. Using this approach, we found four causal genes for the dwarf mutation, the round shape grain mutation and the awnless mutation. Three of these genes were the same as those previously reported, but one was a novel gene involved in awn formation. The novel gene was isolated from Bozu-Aikoku, a mutant of Aikoku with the awnless trait, in which nine polymorphisms were predicted to alter gene function by their whole-genome comparison. Based on the information on gene function and tissue-specific expression patterns of these candidate genes, Os03g0115700/LOC_Os03g02460, annotated as a shortchain dehydrogenase/reductase SDR family protein, is most likely to be involved in the awnless mutation. Indeed, complementation tests by transformation showed that it is involved in awn formation. Thus, this method is an effective way to accelerate genome breeding of various crop species by enabling the identification of useful genes that can be used for crop breeding with minimal effort for NGS analysis.</p>
Whole genome sequencing (WGS) data from invasive pine sawfly Diprion similis
<p>Biological introductions are unintended "natural experiments" that provide unique insights into evolutionary processes. Invasive phytophagous insects are of particular interest to evolutionary biologists studying adaptation, as introductions often require rapid adaptation to novel host plants. However, adaptive potential of invasive populations may be limited by reduced genetic diversity—a problem known as the "genetic paradox of invasions". One potential solution to this paradox is if there are multiple invasive waves that bolster genetic variation in invasive populations. Evaluating this hypothesis requires characterizing genetic variation and population structure in the invaded range. To this end, we assemble a reference genome and describe patterns of genetic variation in the introduced white pine sawfly, <em>Diprion</em> <em>similis</em>. This species was introduced to North America in 1914, where it has rapidly colonized the thin-needled eastern white pine (<em>Pinus</em> <em>strobus</em>), making it an ideal invasion system for studying adaptation to novel environments. To evaluate evidence of multiple introductions, we generated whole-genome resequencing data for 64 <em>D</em>. <em>similis</em> females sampled across the North American range. Both model-based and model-free clustering analyses supported a single population for North American <em>D</em>. <em>similis</em>. Within this population, we found evidence of isolation-by-distance and a pattern of declining heterozygosity with distance from the hypothesized introduction site. Together, these results support a single-introduction event. We consider implications of these findings for the genetic paradox of invasion and discuss priorities for future research in <em>D</em>. <em>similis</em>, a promising model system for invasion biology.</p>
Whole genome sequence data of Lactiplantibacillus plantarum IMI507027
<p>The present data files are the annotation output from the whole genome sequencing of the strain Lactiplantibacillus plantarum IMI507027. </p>
Whole genome sequence variant discovery from Ethiopian Boran, N'Dama and Holstein cattle
<p>Whole genome sequence variants (SNPs) from forty samples of each Ethiopian Boran, N'Dama and Holstein cattle breeds which were utilised in the Assessment of genotyping array performance for genome-wide association studies and imputation in African cattle study by Riggio et al., 2022, (in review). The NDama sequences included samples from Guinea (n =21), Nigeria (n =10) and Senegal (n = 9); and the Boran samples from Ethiopia (n = 30) and Kenya (n = 10). Variants discovery followed the protocol described in the Material and Method section of the paper.</p>
Single cell whole genome sequencing from Funnell, O'Flanagan, Williams et al
<p>This repository provides the processed data necessary to reproduce the results from: "Single cell genomic variation induced by mutational processes in cancer<strong> </strong><em>Funnell, O’Flanagan, Williams et al</em>"</p> <p>This includes the following:</p> <ul> <li>Single cell whole genome sequencing <ul> <li>Allele specific copy number profiles</li> <li>SNV counts per cell</li> <li>Structural variant counts per cell</li> <li>QC metrics</li> <li>clone assignments</li> <li>phylogenetic trees computed with sitka</li> <li>benchmarking results vs other methods</li> </ul> </li> <li>bulk whole genome sequencing <ul> <li>copy number profiles</li> <li>SNVs</li> </ul> </li> <li>10X single cell RNA sequencing <ul> <li>count matrices</li> <li>seurat Rdata objects</li> </ul> </li> <li>analysis tables <ul> <li>downstream processed results used to generate figures</li> </ul> </li> <li>oxford nanopore <ul> <li>phasing results</li> </ul> </li> </ul> <p> </p> <p>For further information please feel free to get in touch with Marc Williams (william1 [at] mskcc.org)</p> <p> </p>
Genotypes of Aedes aegypti mosquitoes derived from SNP chip and low-coverage whole genome sequencing for platform cross-validation
<p>The mosquito <em>Aedes aegypti </em>is the primary vector of many human arboviruses such as dengue, yellow fever, chikungunya, and Zika, which affect millions of people world-wide. Population genetics studies on this mosquito have been important in understanding its invasion pathways and success as a vector of human disease. The Axiom aegypti1 SNP chip was developed from a sample of geographically diverse <em>Ae. aegypti </em>populations to facilitate genomic studies on this species. Here we evaluate the utility of the Axiom aegypti1 SNP chip for population genetics and compare it with a low-depth shot-gun sequencing approach using mosquitoes from the species' native (Africa) and invasive range (outside Africa). These analyses indicate that the results from the SNP chip are highly reproducible and have a higher sensitivity to capture alternative alleles than a low-coverage whole-genome sequencing approach. Although the SNP chip suffers from ascertainment bias, results from population structure, ancestry, demographic, and phylogenetic analyses using the SNP chip were congruent with those derived from low coverage whole genome sequencing, and consistent with previous reports on Africa and outside Africa populations using microsatellites. More importantly, we identified a subset of SNPs that can be reliably used to generate merged databases, opening the door to combined analyses. We conclude that the Axiom aegypti1 SNP chip is a convenient, more accurate, low-cost alternative to low-depth whole genome sequencing for population genetic studies of <em>Ae. aegypti</em> that do not rely on full allelic frequency spectra. Whole genome sequencing and SNP chip data can be easily merged, extending the usefulness of both approaches. </p>
Figure 1 in Genetic difference between two Schistosoma japonicum isolates with contrasting cercarial shedding patterns revealed by whole genome sequencing
Figure 1. Map of geographical locations of research sites.
Single Nucleotide Polymorphisms (SNPs) identified from the whole genome sequences of hilsa shad (Tenualosa ilisha) of the Bay of Bengal
<p>The data file contains 792,939 isolated SNPs identified by discoSnp++ v2.3.x (Uricaru et al., 2015) from the whole genome sequence of T. ilisha of the Bay of Bengal. The central sequence of length 2k-1 is seen in upper case, while the flanking sequences are seen in lower case. SNP_higher/lower: one of the two alleles. id: id of the SNP (each SNP has a unique id).</p> <p>FOR SNPs:</p> <p>P_i:pos_Alt1/Alt2: Information about a ith SNP (If more than a unique SNP is found, the following format is used: P_1:pos_Alt1/Alt2,P_2:pos_Alt1/Alt2,...</p> <p>pos: position of the SNP with respect to the starting position of the bubble, i.e. the starting of the upper case sequence.</p> <p>Alt1: One of the two alleles</p> <p>Alt2: the other</p> <p>FOR INDELs:</p> <p>P_1:pos_size_repeatSize</p> <p>pos: predicted position of the indel with respect to the starting position of the bubble, i.e. the starting of the upper case sequence.</p> <p>size: predicted size of the indel</p> <p>repeatSize: Size of the longest sequence both prefix of the indel and prefix of the sequence located just after the insertion.</p> <p>high/low: sequence complexity. If the sequence if of low complexity (e.g. ATATATATATATATAT) this variable would be low</p> <p>nb_pol: number of polymorphism.</p> <p>left_unitig_length: size of the full left extension.</p> <p>right_unitig_length: size of the right extension.</p> <p>left_contig_length: size of the full left extension.</p> <p>right_contig_length: size of the right extension.</p> <p>C1: number of reads mapping the central upper case sequence from the first read set.</p> <p>C2: number of reads mapping the central upper case sequence from the second read set.</p> <p>Q1 [if reads were given in fastq]: average phred quality of the central nucleotide from the mapped reads from the first read set.</p> <p>Q2 [if reads were given in fastq]: average phred quality of the central nucleotide from the mapped reads from the second read set.</p> <p>G1: Genotype of the variant in the first read set.</p> <p>G2: Genotype of the variant in the second read set.</p> <p>rank: ranks the predictions according to their read coverage in each condition favoring SNPs that are discriminant between conditions.</p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.