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Figure 3 from: Ganjisaffar F, Talamas EJ, Bon MC, Perring TM (2020) First report and integrated analysis of two native Trissolcus species utilizing Bagrada hilaris eggs in California. Journal of Hymenoptera Research 80: 49-70. https://doi.org/10.3897/jhr.80.57024
Figure 3 TCS COI haplotype network for the four clades of T. utahensis by fixing connection limits at 50 steps. Each haplotype is represented by a colored circle. Lines represent one mutational step between haplotypes, and dark circles represent unsampled haplotypes inferred from the data. Interrupted lines were used when haplotypes were separated by a long branch of more than 7 mutation steps.
Figures 23-25 from: Ganjisaffar F, Talamas EJ, Bon MC, Perring TM (2020) First report and integrated analysis of two native Trissolcus species utilizing Bagrada hilaris eggs in California. Journal of Hymenoptera Research 80: 49-70. https://doi.org/10.3897/jhr.80.57024
Figures 23-25 Trissolcus utahensis (FSCA 00091872, ex. B. hilaris) 23 head, anterior view 24 lateral habitus 25 head, mesosoma, metasoma, dorsal view. Scale bars in millimeters.
Figure 2 from: Ganjisaffar F, Talamas EJ, Bon MC, Perring TM (2020) First report and integrated analysis of two native Trissolcus species utilizing Bagrada hilaris eggs in California. Journal of Hymenoptera Research 80: 49-70. https://doi.org/10.3897/jhr.80.57024
Figure 2 The Bayesian 50% majority rule consensus tree inferred from the 56 CO1 sequences of the six Trissolcus species including T. hullensis and T. utahensis. Only posterior probabilities >90% are indicated on the nodes. The tree is rooted with the outgroup Trissolcus thyantae (GenBank MN615574). The scale bar corresponds to 0.1 estimated substitutions per site.
Figures 6-7 from: Ganjisaffar F, Talamas EJ, Bon MC, Perring TM (2020) First report and integrated analysis of two native Trissolcus species utilizing Bagrada hilaris eggs in California. Journal of Hymenoptera Research 80: 49-70. https://doi.org/10.3897/jhr.80.57024
Figures 6-7 Trissolcus cosmopeplae, holotype female (USNMENT00989096) 6 head and mesosoma, lateral view 7 head and mesosoma, dorsal view. Scale bars in millimeters.
Figure 1 from: Ganjisaffar F, Talamas EJ, Bon MC, Perring TM (2020) First report and integrated analysis of two native Trissolcus species utilizing Bagrada hilaris eggs in California. Journal of Hymenoptera Research 80: 49-70. https://doi.org/10.3897/jhr.80.57024
Figure 1 Survey locations are displayed in red dots. The black rectangle shows the Agricultural Operations of the University of California, Riverside, where most of our surveys were conducted. Five Trissolcus hullensis were recovered from an alfalfa field (33.96508°N, 117.34084°W), one Trissolcus utahensis was recovered from a squash field with mustard weeds (33.96611°N, 117.34230°W), and eleven T. utahensis were recovered from roadside mustard weeds (33.99105°N, 117.33360°W).
Figures 26-29 from: Ganjisaffar F, Talamas EJ, Bon MC, Perring TM (2020) First report and integrated analysis of two native Trissolcus species utilizing Bagrada hilaris eggs in California. Journal of Hymenoptera Research 80: 49-70. https://doi.org/10.3897/jhr.80.57024
Figures 26-29 Trissolcus utahensis (FSCA 00033042, ex. P. maculiventris) 26 head, anterior view 27 head and mesosoma, lateral view 28 head and mesosoma, ventrolateral view 29 lateral habitus. Scale bars in millimeters.
Figure 4-5 from: Ganjisaffar F, Talamas EJ, Bon MC, Perring TM (2020) First report and integrated analysis of two native Trissolcus species utilizing Bagrada hilaris eggs in California. Journal of Hymenoptera Research 80: 49-70. https://doi.org/10.3897/jhr.80.57024
Figure 4-5 4Trissolcus hullensis (FSCA 00091886), head, mesosoma, metasoma, dorsolateral view 5T. utahensis (FSCA 00000302), head, mesosoma, metasoma, dorsolateral view. Scale bars in millimeters.
Figures 20-22 from: Ganjisaffar F, Talamas EJ, Bon MC, Perring TM (2020) First report and integrated analysis of two native Trissolcus species utilizing Bagrada hilaris eggs in California. Journal of Hymenoptera Research 80: 49-70. https://doi.org/10.3897/jhr.80.57024
Figures 20-22 Trissolcus utahensis20 FSCA 00033239 (ex. B. hilaris), head, mesosoma, metasoma, dorsal view 21 FSCA 00033041 (ex. P. maculiventris), head, mesosoma, metasoma, dorsolateral view 22 FSCA 00033040 (ex. P. maculiventris), head, mesosoma, metasoma, dorsal view. Scale bars in millimeters.
Figures 16-19 from: Ganjisaffar F, Talamas EJ, Bon MC, Perring TM (2020) First report and integrated analysis of two native Trissolcus species utilizing Bagrada hilaris eggs in California. Journal of Hymenoptera Research 80: 49-70. https://doi.org/10.3897/jhr.80.57024
Figures 16-19 Trissolcus utahensis, head and mesosoma, ventrolateral view 16 DPI_FSCA00033239 (ex. B. hilaris) 17 FSCA 00033041 (ex. P. maculiventris) 18 FSCA 00000302 (ex. P. maculiventris) 19 FSCA 00033040 (e. P. maculiventris). Scale bars in millimeters.
Figures 12-15 from: Ganjisaffar F, Talamas EJ, Bon MC, Perring TM (2020) First report and integrated analysis of two native Trissolcus species utilizing Bagrada hilaris eggs in California. Journal of Hymenoptera Research 80: 49-70. https://doi.org/10.3897/jhr.80.57024
Figures 12-15 Trissolcus utahensis, head and mesosoma, lateral view 12 DPI_FSCA00033239 (ex. B. hilaris) 13 FSCA 00033041 (ex. P. maculiventris) 14 FSCA 00000302 (ex. P. maculiventris) 15 FSCA 00033040 (ex. P. maculiventris). Scale bars in millimeters.
Figures 1-6 from: Li Q, Wang C, Hu H-Y (2021) Two new species of Dzhanokmenia (Hymenoptera, Eulophidae) from China, with first report on a host association for the genus. ZooKeys 1009: 67-79. https://doi.org/10.3897/zookeys.1009.57556
Figures 1-6 Dzhanokmenia stefaniolae, female (holotype): 1 habitus, dorsal 2 mesosoma, lateral 3 head, frontal 4 head, dorsal 5 wings 6 metasoma, dorsal. Scale bars: 0.2 mm.
Figures 19-26 from: Li Q, Wang C, Hu H-Y (2021) Two new species of Dzhanokmenia (Hymenoptera, Eulophidae) from China, with first report on a host association for the genus. ZooKeys 1009: 67-79. https://doi.org/10.3897/zookeys.1009.57556
Figures 19-26 Dzhanokmenia yuxuannis, female (19–22 holotype 23–26 paratypes): 19 habitus, lateral 20 body, dorsal 21 head, frontal 22 wings 23, 25 metasoma, dorsal 24, 26 metasoma, ventral. Scale bars: 0.2 mm.
Figures 13-18 from: Li Q, Wang C, Hu H-Y (2021) Two new species of Dzhanokmenia (Hymenoptera, Eulophidae) from China, with first report on a host association for the genus. ZooKeys 1009: 67-79. https://doi.org/10.3897/zookeys.1009.57556
Figures 13-18 13–17Stefaniola sp.: 13 larva 14, 15 pupa 16, 17 adult 18 emergence hole of adult Dzhanokmenia stefaniolae. Scale bars: 1 mm.
Figures 7-12 from: Li Q, Wang C, Hu H-Y (2021) Two new species of Dzhanokmenia (Hymenoptera, Eulophidae) from China, with first report on a host association for the genus. ZooKeys 1009: 67-79. https://doi.org/10.3897/zookeys.1009.57556
Figures 7-12 Dzhanokmenia stefaniolae, female (paratypes): 7, 9, 11 metasoma, dorsal 8, 10, 12 metasoma, ventral. Scale bars: 0.2 mm.
FIGURE 4 in Report of a new genus Mirigryllus belonging to tribe Modicogryllini, with a new species M. nigrus from Zhejiang, China (Orthoptera: Gryllidae: Gryllinae Modicogryllini)
FIGURE 4. Calling song of Mirigryllus nigrus. Wave of songs with 1s and 0.1s scale bar.
Data from: The REporting of studies Conducted using Observational Routinely-collected health Data (RECORD) statement: methods for arriving at consensus and developing reporting guidelines
Objective: Routinely collected health data, collected for administrative and clinical purposes, without specific a priori research questions, are increasingly used for observational, comparative effectiveness, health services research, and clinical trials. The rapid evolution and availability of routinely collected data for research has brought to light specific issues not addressed by existing reporting guidelines. The aim of the present project was to determine the priorities of stakeholders in order to guide the development of the REporting of studies Conducted using Observational Routinely-collected health Data (RECORD) statement. Methods: Two modified electronic Delphi surveys were sent to stakeholders. The first determined themes deemed important to include in the RECORD statement, and was analyzed using qualitative methods. The second determined quantitative prioritization of the themes based on categorization of manuscript headings. The surveys were followed by a meeting of RECORD working committee, and re-engagement with stakeholders via an online commentary period. Results: The qualitative survey (76 responses of 123 surveys sent) generated 10 overarching themes and 13 themes derived from existing STROBE categories. Highest-rated overall items for inclusion were: Disease/exposure identification algorithms; Characteristics of the population included in databases; and Characteristics of the data. In the quantitative survey (71 responses of 135 sent), the importance assigned to each of the compiled themes varied depending on the manuscript section to which they were assigned. Following the working committee meeting, online ranking by stakeholders provided feedback and resulted in revision of the final checklist. Conclusions: The RECORD statement incorporated the suggestions provided by a large, diverse group of stakeholders to create a reporting checklist specific to observational research using routinely collected health data. Our findings point to unique aspects of studies conducted with routinely collected health data and the perceived need for better reporting of methodological issues.
Data from: Transgender transitioning and change of self-reported sexual orientation
Objective: Sexual orientation is usually considered to be determined in early life and stable in the course of adulthood. In contrast, some transgender individuals report a change in sexual orientation. A common reason for this phenomenon is not known. Methods: We included 115 transsexual persons (70 male-to-female "MtF" and 45 female-to-male "FtM") patients from our endocrine outpatient clinic, who completed a questionnaire, retrospectively evaluating the history of their gender transition phase. The questionnaire focused on sexual orientation and recalled time points of changes in sexual orientation in the context of transition. Participants were further asked to provide a personal concept for a potential change in sexual orientation. Results: In total, 32.9% (n = 23) MtF reported a change in sexual orientation in contrast to 22.2% (n = 10) FtM transsexual persons (p = 0.132). Out of these patients, 39.1% (MtF) and 60% (FtM) reported a change in sexual orientation before having undergone any sex reassignment surgery. FtM that had initially been sexually oriented towards males ( = androphilic), were significantly more likely to report on a change in sexual orientation than gynephilic, analloerotic or bisexual FtM (p = 0.012). Similarly, gynephilic MtF reported a change in sexual orientation more frequently than androphilic, analloerotic or bisexual MtF transsexual persons (p = 0.05). Conclusion: In line with earlier reports, we reveal that a change in self-reported sexual orientation is frequent and does not solely occur in the context of particular transition events. Transsexual persons that are attracted by individuals of the opposite biological sex are more likely to change sexual orientation. Qualitative reports suggest that the individual's biography, autogynephilic and autoandrophilic sexual arousal, confusion before and after transitioning, social and self-acceptance, as well as concept of sexual orientation itself may explain this phenomenon.
Data from: PCR duplication: a one-step cloning-free method to generate duplicated chromosomal loci and interference-free expression reporters in yeast
Here, we report on a novel PCR targeting-based strategy called 'PCR duplication' that enables targeted duplications of genomic regions in the yeast genome using a simple PCR-based approach. To demonstrate its application we first duplicated the promoter of the FAR1 gene in yeast and simultaneously inserted a GFP downstream of it. This created a reporter for promoter activity while leaving the FAR1 gene fully intact. In another experiment, we used PCR duplication to increase the dosage of a gene in a discrete manner, from 1× to 2x. Using TUB4, the gene encoding for the yeast γ-tubulin, we validated that this led to corresponding increases in the levels of mRNA and protein. PCR duplication is an easy one-step procedure that can be adapted in different ways to permit rapid, disturbance-free investigation of various genomic regulatory elements without the need for ex vivo cloning.
Data from: Mandated reporters' experiences with reporting child maltreatment: a meta-synthesis of qualitative studies
Objective: To systematically search for research about the effectiveness of mandatory reporting of child maltreatment and to synthesize qualitative research that explores mandated reporters' (MRs) experiences with reporting. Design: As no studies assessing the effectiveness of mandatory reporting were retrieved from our systematic search, we conducted a meta-synthesis of retrieved qualitative research. Searches in Medline (OVID), Embase, PsycINFO, CINAHL, Sociological Abstracts, ERIC, Criminal Justice Abstracts, and Cochrane Library yielded over 6000 citations, which were deduplicated and then screened by two independent reviewers. English-language, primary qualitative studies that investigated MRs' experiences with reporting of child maltreatment were included. Critical appraisal involved a modified checklist from the Critical Appraisal Skills Programme (CASP) and qualitative meta-synthesis was used to combine results from the primary studies. Setting: All healthcare and social-service settings implicated by mandatory reporting laws were included. Included studies crossed nine high-income countries (United States, Australia, Sweden, Taiwan, Canada, Norway, Finland, Israel and Cyprus) and three middle-income countries (South Africa, Brazil, and El Salvador). Participants: The studies represent the views of 1088 MRs. Outcomes: Factors that influence MRs' decision to report and MRs' views towards and experiences with mandatory reporting of child maltreatment. Results: Forty-four articles reporting 42 studies were included. Findings indicate that MRs struggle to identify and respond to less overt forms of child maltreatment. While some articles (14%) described positive experiences MRs had with the reporting process, negative experiences were reported in 73% of articles and included accounts of harm to therapeutic relationships and child death following removal from their family of origin. Conclusions: The findings of this meta-synthesis suggest that there are many potentially harmful experiences associated with mandatory reporting and that research on the effectiveness of this process is urgently needed.
Data from: Assessment of the quality of reporting in randomized controlled trials of acupuncture in the Korean literature using the CONSORT statement and the STRICTA guidelines
Objectives: This study aimed to assess the completeness of reporting of RCTs of acupuncture in the Korean literature. Design: Systematic review. Methods: We searched 12 Korean databases and 7 Korean journals to identify eligible RCTs of acupuncture published from 1996 to July 2011. We used the Consolidated Standards of Reporting Trials (CONSORT) checklist for parallel RCTs and the revised STandards for Reporting Interventions in Clinical Trials of Acupuncture (STRICTA) to assess the quality of reporting in Korean RCTs. We compared the completeness of reporting of CONSORT and STRICTA items in RCTs published in two time periods (1996-2004 referred to as the early period and 2005-2011 referred to as the late period). Results: We analysed 146 eligible RCTs using the CONSORT statement concerning RCTs of both needling and non-needling acupuncture and the STRICTA guidelines for 90 trials of needling acupuncture. Among the 103 RCTs in the late period, the proportion of RCTs that completely reported the CONSORT items of outcome definition (15.5%), sample size calculation (2.9%), randomisation (56%), allocation concealment (5.8%), implementation of allocation (11.7%), outcome assessor blinding (20.3%), flow of participants (25.2%), number of participants analysed (19.4%), ancillary analyses (0.0%), adverse events (24.3%), generalisability of findings (1.9%) and overall evidence (32.0%) remained small. Among the 61 RCTs of needling acupuncture in the late period, the STRICTA items of setting/context (24.6%) and practitioner background (27.9%) showed incomplete reporting. The completeness of reporting improved over time in several CONSORT and STRICTA items. Conclusion: The completeness of reporting of Korean RCTs of acupuncture was suboptimal according to the CONSORT and revised STRICTA statements. Trial authors and journal editors should use the CONSORT statement and the STRICTA guidelines for transparent reporting in Korean RCTs of acupuncture. The endorsement of the CONSORT and revised STRICTA statements in author instructions is also required.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.