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FIGURE 22 in Revision of Neotrichobia Tuttle & Baker (Acari, Tetranychidae) with ontogenetic development and redescription of N. arizonensis

FIGURE 22. Neotrichobia arizonensis Tuttle & Baker, larva, legs, A, leg I, B, pretarsus I, C, leg, II, D, leg III, E, ventral seta of tarsus III.

FIGURE 6 in Redescription of three species of Aponychus from China with ontogenetic development and morphological variations of A. corpuzae (Acariformes: Tetranychidae)

FIGURE 6. Aponychus corpuzae. Female: A, femur and genu of leg Ⅰ; B, tibia and tarsus of leg Ⅰ; C, femur and genu of leg II; D, tibia and tarsus of leg II; E, trochanter, femur and genu of leg III; F, tibia and tarsus of leg III; G, trochanter, femur and genu of leg IV; H, tibia and tarsus of leg IV.

FIGURE 9 in Revision of Neotrichobia Tuttle & Baker (Acari, Tetranychidae) with ontogenetic development and redescription of N. arizonensis

FIGURE 9. Neotrichobia arizonensis Tuttle & Baker, male, legs, A, trochanter, femur and genu of leg I, B, tibia and tarsus of leg I; C, leg II; D, leg III, E, leg IV.

FIGURE 5. Aponychus corpuzae. A in Redescription of three species of Aponychus from China with ontogenetic development and morphological variations of A. corpuzae (Acariformes: Tetranychidae)

FIGURE 5. Aponychus corpuzae. A, female, palp; B, male, palp; C, deutonymph, palp; D, protonymph, palp; E, larva, palp.

FIGURE 18 in Revision of Neotrichobia Tuttle & Baker (Acari, Tetranychidae) with ontogenetic development and redescription of N. arizonensis

FIGURE 18. Neotrichobia arizonensis Tuttle & Baker, deutonymph, legs, A, leg I, B, leg, II, C, leg III, D, leg IV.

FIGURE 14 in Revision of Neotrichobia Tuttle & Baker (Acari, Tetranychidae) with ontogenetic development and redescription of N. arizonensis

FIGURE 14. Neotrichobia arizonensis Tuttle & Baker, deutonymph, legs, A, leg I, B, leg, II, C, leg III, D, leg IV.

FIGURE 17 in Revision of Neotrichobia Tuttle & Baker (Acari, Tetranychidae) with ontogenetic development and redescription of N. arizonensis

FIGURE 17. Neotrichobia arizonensis Tuttle & Baker, protonymph, A, stylophore; B, peritreme, C, palp, dorsal view; D, palp ventral view.

Fig. 4 in Suspension cell secretome of the grain legume Lathyrus sativus (grasspea) reveals roles in plant development and defense responses

Fig. 4. Physicochemical assessment of the grasspea suspension secretome (GSS), including pI (A), molecular weight (in kDa) (B), and hydrophilicity (C), with respect to MSS, DSS and LSS (MSS corresponds to the monocot suspension secretome, DSS to the dicot suspension secretome and LSS to the lower plant suspension secretome).

Fig. 2 in Suspension cell secretome of the grain legume Lathyrus sativus (grasspea) reveals roles in plant development and defense responses

Fig. 2. Generation of grasspea calli, establishment of suspension culture and isolation of the grasspea suspension secretome (GSS). (A) Root-cut and shoot-cut embryo axes were employed for the generation of 4-week-old calli, which were bulked together in a suspension culture. (B) Microscopic examination of suspension cells and viability assessment using Evans blue (left panel) and FDA (right panel). (C) Quantitative analysis of physicochemical properties including changes in pH in the suspension culture, fresh weight (FW), dry weight (DW), soluble sugars and total protein. (D) Protein SDS-PAGE profile of the grasspea secretome. Lane 1 represents the molecular weight marker (MW). Purity evaluation of grasspea secreted fraction using (E) catalase activity and (F) western blotting with anti-RbcL (Supplementary Fig. S1). Relative catalase activities are presented as mean ± SE of triplicate experiments.

Fig. 3 in Suspension cell secretome of the grain legume Lathyrus sativus (grasspea) reveals roles in plant development and defense responses

Fig. 3. Overview of total grasspea suspension secreted (GSS) proteins and prediction of mode of secretion and (A) localization using multiple tools (B). Comparison of shared and distinct GSS proteins, first (C) with respect to total in vitro secretome (IVS) and in planta secretome (IPS) and second (D) compared to the in vitro suspension culture secretome reported in monocots, dicots, and lower plants, abbreviated as MSS, DSS and LSS, respectively (MSS corresponds to monocot suspension secretome, DSS to dicot suspension secretome and LSS to lower plant suspension secretome).

Fig. 1 in Suspension cell secretome of the grain legume Lathyrus sativus (grasspea) reveals roles in plant development and defense responses

Fig. 1. Schematic representation of the experimental design and workflow of the establishment of the grasspea suspension secretome (GSS). Proteomic profiling was accomplished by generating suspension culture and sequential assessment of physicochemical properties and protein identification.

Fig. 6 in Suspension cell secretome of the grain legume Lathyrus sativus (grasspea) reveals roles in plant development and defense responses

Fig. 6. Localization validation of endochitinase (S597) and G-type lectin S-receptor-like serine threonine kinase (S718). The panels include (A) expression of YFPtagged S597 in onion epidermal cells, (B) plasmolysis of S597-transformed onion peel (C), expression of YFP-tagged S718 in onion peel cells and (D) plasmolyzed onion peel cells expressing YFP-tagged S718. A pSITE3CA empty vector control was also monitored besides the target genes (E).

opencc-by-4.0Aug 2023View details →

Developers Talking About Code Quality: study design and dataset

Electronic supplement for the article Developers Talking About Code Quality Please refer to the following paper when you cite/use this data: J. Börstler, K.E. Bennin, S. Hooshangi, J. Jeuring, H. Keuning, C. Kleiner, B. MacKellar, R. Duran, H. Störrle, D. Toll, J van Assema. Developers Talking About Code Quality. Empirical Software Engineering, 2023. https://link.springer.com/article/10.1007/s10664-023-10381-0 Overview of the electronic supplement (sorted according to file names) <ol> <li>The data <ol> <li>data_all-except-Q4.xlsx The original cleaned data for Questions Q1-Q3 and Q5-Q11.</li> <li>data_Q4 assertions.xlsx The "assertions" which are based on the transcripts of Q4.</li> </ol> </li> <li>The information provided to study participants <ol> <li>participants_consent-form_EN.pdf The consent form the participants signed (English version, interviewees received a copy on request).</li> <li>participants_interview-info.pdf The information sheet that was handed out for recruiting interview participants (they could keep this form).</li> </ol> </li> <li>The study design and guidelines <ol> <li>study-design_coding-guide_birds-eye-summaries.pdf The guidelines for coding the transcripts of Q4 into "assertions" (which we call "birds-eye summaries" in these guidelines).</li> <li>study-design_main.pdf The main study guide comprising (among others) a detailed interview script.</li> <li>study-design_transcription.pdf The guidelines used for transcribing Q4.</li> </ol> </li> </ol>

Fig. 8 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases

Fig. 8. HPLC chromatogram of the mogrol standard and Agrobacterium-mediated transient expression of fruit harbouring pTRV-SgCPRs and pTRV-0 at 24 h after agroinfiltration.

Fig. 9 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases

Fig. 9. (A) Mogrol content in the S. grosvenorii fruit harbouring PBI121, PBI121-SgCPR1, and PBI121-SgCPR2. (B) MIIE content in the S. grosvenorii fruit harbouring PBI121, PBI121-SgCPR1, and PBI121-SgCPR2. (C) MIII content in the S. grosvenorii fruit harbouring PBI121, PBI121-SgCPR1, and PBI121-SgCPR2. (D) Accumulation of mogrol in the S. grosvenorii fruit harbouring pTRV, pTRV-SgCPR1, and pTRV-SgCPR2. (E) Accumulation of MII in the S. grosvenorii fruit harbouring pTRV, pTRV- SgCPR1, and pTRV-SgCPR2. (F) Accumulation of MIII in the S. grosvenorii fruit harbouring pTRV, pTRV-SgCPR1, and pTRV-SgCPR2. The values are the mean ± SD of three independent biological replicates.

Fig. 7 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases

Fig. 7. HPLC chromatogram of the mogrol standard and Agrobacterium-mediated transient expression of fruit harbouring PBI121-SgCPRs and PBI121 at 24 h after agroinfiltration.

Fig. 2 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases

Fig. 2. (A) Cloning of full-length and specific fragments of the SgCPR1 and SgCPR2 genes from S. grosvenorii cDNA. M, marker (2000 bp). The fulllength SgCPR1 and SgCPR2 genes are shown in red brackets, whereas the blue arrow indicates the specific fragments of the SgCPR1 and SgCPR2 genes. (B) Vector map of PBI121. (C) Construction of PBI12- SgCPR recombinant plasmids with the GUS marker and CaMV35S promoter. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 5 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases

Fig. 5. qRT-PCR analysis of SgCPR1 and SgCPR2 in fruit after infiltration (at t = 0 h). Relative expression levels were normalized to t = 0 h. (A) Relative transcript levels of SgCPR1 in fruit harbouring PBI121- SgCPR1 and PBI121. (B) SgCPR2 gene expression levels in fruit harbouring PBI121 and PBI121-SgCPR2. (C) Relative expression levels of SgCPR1 in fruit harbouring pTRV-SgCPR1 and pTRV-0. (D) Expression levels of SgCPR2 in fruit harbouring pTRV-SgCPR2 and pTRV-0. The relative expression levels normalized to the level of the SgUBQ gene. All the data are shown as means±SDs. * indicates significant differences at p <0.05 (LSD test).

Fig. 3 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases

Fig. 3. (A) Selective nucleotide sequences for VIGS in S. grosvenorii, which were inserted into EcoRI and BamHI. (B) pTRV2-SgCPR recombinant plasmid construction.

Fig. 1 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases

Fig. 1. The mogroside biosynthetic pathway in S. grosvenorii. The blue box on the left shows the generation of mogrol, which is a unique nonglycosylated tetra-hydroxycucurbitane in S. grosvenorii. The red box on the right represents a series of glycosylation reactions. The enzyme names are abbreviated as follows: SgSQE, squalene epoxidase; SgCS, cucurbitadienol synthase; SgEPH, epoxide hydrolase; SgCYP450, cytochrome P450 monooxygenase; SgCPR, NADPHcytochrome P450 reductase; SgUGT, UDP-glucosyltransferase. Among these, the candidate SgCPRs are highlighted in red. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)