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opennotspecifiedSep 2021View details →

Fig. 6 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases

Fig. 6. Accumulation of MIII and MIIE. (A) Total ion chromatograms of ten standard mixtures determined by LC-MS/MS. Among these, the retention times of MIII and MIIE were 10.01 and 12.09 min, respectively. (B) Extracted ion chromatograms of the sample. The red arrow indicates the Q1/Q3 mass chromatograms of MIII, which are in the red box. The Q1/Q3 mass chromatograms of MIIE are shown in the blue box, as indicated by the blue arrow. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

opennotspecifiedSep 2021View details →

Fig. 4 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases

Fig. 4. Histochemical GUS assay of transient expression in S. grosvenorii. GUS expression was detected in fruit but not in CK or leaves.

Fig. 5 in Unique localization of jasmonic acid-related compounds in developing Phaseolus vulgaris L. (common bean) seeds revealed through desorption electrospray ionization-mass spectrometry imaging

Fig. 5. DESI-MS/MSI of OPDA and OPC-8 in the developing Phaseolus vulgaris seeds. (a) Optical image of the seed section for OPDA analysis. (b) MS/MS spectrum of precursor ion at m/z 291.1966 ± 1 Da obtained at the target enhanced mode for m/z 165.1. (c) Ion image at m/z 165.1300. (d) Optical image of the seed section for OPC-8:0 analysis. (e) MS/MS spectrum of precursor ion at m/z 293.2122 ± 1 Da obtained at the target enhanced mode for m/z 225.1. Ion images at m/z (f) 223.1400 and (g) 231.2142. Scale bar = 2 mm. Compound names are defined in Table 1.

Fig. 4 in Unique localization of jasmonic acid-related compounds in developing Phaseolus vulgaris L. (common bean) seeds revealed through desorption electrospray ionization-mass spectrometry imaging

Fig. 4. LC-ESI-MS/MS analysis of JA-related compounds in the extracts from the radicle and seed coat of developing Phaseolus vulgaris seeds. MS/MS spectra of peaks at (a) 5.3 min in Fig. 3c, (b) 5.3 min in Fig. 3d, (c) 6.5 min in Fig. 3c and (d) 6.5 min in Fig. 3d and (e) 6.3 min in Fig. 3e and (f) 6.3 min in Fig. 3f and (g) 6.4 min in Fig. 3e, (h) 6.4 min in Fig. 3f, (i) 6.7 min in Fig. 3e, and (j) 6.7 min in Fig. 3f. Compound names are defined in Table 1.

Fig. 2 in Unique localization of jasmonic acid-related compounds in developing Phaseolus vulgaris L. (common bean) seeds revealed through desorption electrospray ionization-mass spectrometry imaging

Fig. 2. LC-ESI-MS/MS analysis of JA-related compound standards. Spectra of (a) OPDA, (b) OPC-8:0, and (c) JA standards. Compound names are defined in Table 1.

Fig. 1 in Unique localization of jasmonic acid-related compounds in developing Phaseolus vulgaris L. (common bean) seeds revealed through desorption electrospray ionization-mass spectrometry imaging

Fig. 1. DESI-MSI analysis of JA-related compounds in the developing Phaseolus vulgaris seeds. (a) Optical image of the section. (b) Mass spectrum obtained from the section. Ion images of m/z (c) 277.2172, (d) 291.1953, and (e) 293.2117. Three different developing seeds were analyzed, and the results from one are shown as representative data. Scale bar = 2 mm. Compound names are defined in Table 1.

Fig. 3 in Unique localization of jasmonic acid-related compounds in developing Phaseolus vulgaris L. (common bean) seeds revealed through desorption electrospray ionization-mass spectrometry imaging

Fig. 3. LC-ESI-MS analysis of JA-related compounds in the extracts from the radicle and seed coat of developing Phaseolus vulgaris seeds. Base peak chromatogram of m/z 277.2173 ±10 ppm for (a) radicle and (b) seed coat, m/z 291.1966 ± 10 ppm for (c) radicle and (d) seed coat, and m/z 293.2122 ± 10 ppm for (e) radicle and seed coat, respectively. Peaks with arrow indicates JA-related compounds: (a) and (b) αLA, (c) and (d) OPDA, and (e) and (f) OPC-8:0. Compound names are defined in Table 1.

Figure 5 in A comparative account of the embryonic lineage and gonad development in two species of Rhabditidae (Nematoda)

Figure 5. Schematic and LM micrograph showing sequence of vulva formation in Cephaloboides anisospiculus (a, c, e, g, i, k, m) and Mesorhabditis paucipapillata (b, d, f, h, j, l, n). C, D: Second-stage juvenile showing 12 Pn.ps; e, f: third-stage juvenile showing 12 vulval precursor cells with five cells forming vulval prominence group; i, j: fourth-stage juvenile showing vulval prominence group aggregating at vulval site; k, l: moulting fourth-stage juvenile with vulval precursor cells; m, n: vulval region: o, p: SEM of vulval region. Scale bars = 10 µm.

Figure 2 in A comparative account of the embryonic lineage and gonad development in two species of Rhabditidae (Nematoda)

Figure 2. Embryonic stages in Mesorhabditis paucipapillata. a: Egg showing pronuclei before fusion (arrow pointing to the male pronucleus); b: two-celled stage; c: four-celled stage; d: rhomboid arrangement of blastomeres; e: five-celled stage; f: eight-celled stage; g: sixteen-celled stage; h, i: morula stage; j: blastula stage; k: gastrula stage; l: lima bean stage; m: comma stage; n: tadpole stage; o: plum stage; p: pretzel stage. Scale bar = 10 µm.

Figure 4 in A comparative account of the embryonic lineage and gonad development in two species of Rhabditidae (Nematoda)

Figure 4. Gonad development in Mesorhabditis paucipapillata. Genital primordium a–e, h, i. a: Firststage juvenile; b: moulting second-stage male juvenile; c: moulting second-stage female juvenile; d: third-stage male juvenile; e: third-stage female juvenile; f: tail of third-stage male juvenile showing spicular primordium; g: tail of third-stage female juvenile; h: fourth-stage male juvenile; i: fourth-stage female juvenile; j: adult male gonad; k: adult female gonad. Scale bars = 10 µm.

Figure 7 in A comparative account of the embryonic lineage and gonad development in two species of Rhabditidae (Nematoda)

Figure 7. Gonad development in Cephaloboides anisospiculus. Genital primordium a–e, h, i. a: Firststage juvenile; b: moulting second-stage male juvenile; c: moulting second-stage female juvenile; d: third-stage male juvenile; e: third-stage female juvenile; f: tail of third-stage male juvenile showing spicular primordium; g: tail of third-stage female juvenile; h: fourth-stage male juvenile; i: fourth-stage female juvenile; j: adult male gonad; k, l: adult female genital branch. Scale bar = 10 µm.

Figure 1 in A comparative account of the embryonic lineage and gonad development in two species of Rhabditidae (Nematoda)

Figure 1. Early embryonic cell lineages in Mesorhabditis paucipapillata and Cephaloboides anisospiculus as compared to Caenorhabditis elegans.

Figure 6 in A comparative account of the embryonic lineage and gonad development in two species of Rhabditidae (Nematoda)

Figure 6. Embryonic stages in Cephaloboides anisospiculus. a: Single-celled egg; b: two-celled stage; c: four-celled stage; d: rhomboid arrangement of blastomeres; e: six-celled stage; f: ten-celled stage; g: sixteen-celled stage; h: 20–24 celled stage; i: blastula stage; j: lima bean stage; k: comma stage; l: tadpole stage: m: plum stage; n: loop stage; o, p: pretzel stage. Scale bar = 10 µm.

Figure 3 in A comparative account of the embryonic lineage and gonad development in two species of Rhabditidae (Nematoda)

Figure 3. Duration of landmark stages during embryonic development of Mesorhabditis paucipapillata and Cephaloboides anisospiculus.

opencc-by-4.0Aug 2023View details →

Creative Person and the Development of Creativity: A Challenge For Contemporary Society

<p>Creative Person and the Development of Creativity: A Challenge For Contemporary Society, International Conference, Lobachevsky University of Nizhni Novgorod, University of Rome Foro Italico, January, 19, 2023. Scientific Committee: Irina Petrova, Irina Leonova, Emanuele Isidori</p>

Fig. 6 in Variation in production of cyanogenic glucosides during early plant development: A comparison of wild and domesticated sorghum

Fig. 6. Proportion of nitrogen allocated to dhurrin and nitrate (NO3) in dried, finely ground tissues of S. bicolor, S. brachypodum and S. macrospermum plants at 35 d post-germination. A) Dhurrin allocation; B) Nitrate allocation; C) C:N ratio. Graphs show mean ± 1 standard error (n = 3). Columns with different letters within each tissue are significantly different (p <0.05).

Fig. 3 in Variation in production of cyanogenic glucosides during early plant development: A comparison of wild and domesticated sorghum

Fig. 3. Leaf characteristics of S. bicolor, S. brachypodum and S. macrospermum plants at six harvest points during the first 35 d post-germination. A) Total leaf number; B) Total leaf area (TLA); C) Specific leaf area (SLA); D) Leaf area ratio (LAR). Graphs show mean ± 1 standard error (n = 5), with statistically significant differences indicated at each time point: *p <0.05.

Fig. 5 in Variation in production of cyanogenic glucosides during early plant development: A comparison of wild and domesticated sorghum

Fig. 5. Tissue-specific hydrogen cyanide potential (HCNp, mg HCN per g dw 1) and morphology of individual A) S. bicolor, B) S. brachypodum and C) S. macrospermum plants at six time points during seedling development. The HCNp of a section of the sheath, roots, and each individual leaf was measured at 3, 7, 14, 21, 28 and 35 days (D) post-germination. Colour scale indicates HCNp, used as a proxy for dhurrin concentration (green = low; red = high). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 4 in Variation in production of cyanogenic glucosides during early plant development: A comparison of wild and domesticated sorghum

Fig. 4. Hydrogen cyanide potential (HCNp, mg HCN per g dw 1) and concentration of nitrate (NO) in dried, finely ground tissues of S. bicolor, S. brachypodum and 3 S. macrospermum plants at six harvest points during the first 35 d post-germination (at 35 dpg only for NO3). A) Leaf HCNp; B) Sheath HCNp; C) Root HCNp; D) Total NO3. Graphs show mean ± 1 standard error (n = 5), with statistically significant differences indicated at each time point: *p <0.05. Columns with different letters within each tissue are significantly different (p <0.05). Data for leaf HCNp at 3 days post-germination not shown as a true leaf had not emerged at this stage.

Fig. 1 in Variation in production of cyanogenic glucosides during early plant development: A comparison of wild and domesticated sorghum

Fig. 1. Known geographic distribution of the two wild Sorghum species S. brachypodum and S. macrospermum and the site of collection of the accessions examined in the current study. Seeds were obtained from the Australian Grains Genebank (AGG), Horsham, Victoria. Occurrence records of S. brachypodum and S. macrospermum were obtained from the Atlas of Living Australia (ALA), htt p://www.ala.org.au. Each blue circle represents an occurrence record of S. brachypodum and each orange circle represents S. macrospermum (circled). Collection localities of individual accessions examined here are marked by darker coloured circles. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)