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6,383 results for “isolates”
Data from: Cost effective microsatellite isolation and genotyping by high throughput sequencing
High throughput sequencing (HTS) has emerged as a valuable tool for the rapid isolation of genetic markers for population genetics and pedigree analysis. HTS-based SNP (single nucleotide polymorphism) genotyping protocols like RAD (Restriction-site associated DNA) sequencing or hybrid capture, allow for the isolation of thousands of markers from any non-model organism. However, these protocols are relatively laborious and expensive and the resulting high marker density is not always necessary. Since HTS technology has also greatly simplified the isolation and genotyping process of microsatellite markers, we develop microsatellite markers as a cost efficient and simple alternative to SNP genotyping. We present low coverage genome sequencing data from seven distantly related spider species (Argiope bruennichi, Larinia jeskovi, Oedothorax restusus, Pisaura mirabilis, Australomisidia ergandros, Cheiracanthium punctorium, Theridion grallator) and show the utility of HTS for microsatellite isolation. We also present a simple Illumina amplicon sequencing protocol to genotype microsatellites from multiplex PCR amplicons in the Hawaiian happy face spider T. grallator. We discuss advantages and drawbacks of the use of microsatellites for a range of research questions, and highlight an unexpectedly fast decay and gain of repeat loci for T. grallator.
Data from: Genetic mapping of two components of reproductive isolation between two sibling species of moths, Ostrinia nubilalis and O. scapulalis
We report the quantitative trait loci (QTL) mapping of reproductive isolation traits between Ostrinia nubilalis (the European corn borer) and its sibling species O. scapulalis (the Adzuki bean borer), focusing on two traits: mating isolation (mi) and pheromone production (Pher). Four genetic maps were generated from two backcross families, with two maps (one chromosomal map and one linkage map) per backcross. We located 165–323 AFLP markers on these four maps, resulting in the identification of 27–31 linkage groups, depending on the map considered. No-choice mating experiments with the offspring of each backcross led to the detection of at least two QTLs for mi in different linkage groups. QTLs underlying Pher were located in a third linkage group. The Z heterochromosome was identified by a specific marker (Tpi) and did not carry any of these QTLs. Finally, we considered the global divergence between the two sibling species, distortions of segregation throughout the genome, and the location and effect of mi and Pher QTLs in light of the known candidate genes for reproductive isolation within the genus Ostrinia and, more broadly, in phytophagous insects.
Data from: Spatial and temporal genetic structure of a river-resident Atlantic salmon (Salmo salar) after millennia of isolation
The river-resident Salmo salar ("småblank") has been isolated from other Atlantic salmon populations for 9,500 years in upper River Namsen, Norway. This is the only European Atlantic salmon population accomplishing its entire life cycle in a river. Hydropower development during the last six decades has introduced movement barriers and changed more than 50% of the river habitat to lentic conditions. Based on microsatellites and SNPs, genetic variation within småblank was only about 50% of that in the anadromous Atlantic salmon within the same river. The genetic differentiation (FST) between småblank and the anadromous population was 0.24. This is similar to the differentiation between anadromous Atlantic salmon in Europe and North America. Microsatellite analyses identified three genetic subpopulations within småblank, each with an effective population size Ne of a few hundred individuals. There was no evidence of reduced heterozygosity and allelic richness in contemporary samples (2005–2008) compared with historical samples (1955–56 and 1978–79). However, there was a reduction in genetic differentiation between sampling localities over time. SNP data supported the differentiation of småblank into subpopulations and revealed downstream asymmetric gene flow between subpopulations. In spite of this, genetic variation was not higher in the lower than in the upper areas. The meta-population structure of småblank probably maintains genetic variation better than one panmictic population would do, as long as gene flow among subpopulations is maintained. Småblank is a unique endemic island population of Atlantic salmon. It is in a precarious situation due to a variety of anthropogenic impacts on its restricted habitat area. Thus, maintaining population size and avoiding further habitat fragmentation are important.
Data from: Long distance dispersal and genetic structure of natural populations: an assessment of the inverse isolation hypothesis in peat mosses
It is well accepted that the shape of the dispersal kernel, especially its tail, has a substantial effect on the genetic structure of species. Theory predicts that dispersal by fat-tailed kernels reshuffles genetic material and thus preserves genetic diversity during colonization. Moreover, if efficient long distance dispersal is coupled with random colonization, an inverse isolation effect is predicted to develop in which increasing genetic diversity per colonizer is expected with increasing distance from a genetically variable source. By contrast, increasing isolation leads to decreasing genetic diversity when dispersal is via thin-tailed kernels. Here we use a well-established model group for dispersal biology (peat mosses: genus Sphagnum) with a fat-tailed dispersal kernel, and the natural laboratory of the Stockholm archipelago to study the validity of the inverse isolation hypothesis in spore-dispersed plants in island colonization. Population genetic structure of three species (S. fallax, S. fimbriatum and S. palustre) with contrasting life histories and ploidy levels were investigated on a set of islands using microsatellites. Our data show (φ'st, AMOVA, IBD) that dispersal of the two most abundant species can be well approximated by a random colonization model. We find that genetic diversity per colonizer on islands increases with distance from the mainland for S. fallax and S. fimbriatum. By contrast, S. palustre deviates from this pattern, owing to its restricted distribution in the region affecting its source pool strength. Therefore, the inverse isolation effect appears to hold in natural populations of peat mosses and, likely, in other organisms with small diaspores.
Data from: Origin of a cryptic lineage in a threatened reptile through isolation and historical hybridization
Identifying phylogenetically distinct lineages and understanding the evolutionary processes by which they have arisen are important goals of phylogeography. This information can also help define conservation units in endangered species. Such analyses are being transformed by the availability of genomic-scale data sets and novel analytical approaches for statistically comparing different historical scenarios as causes of phylogeographic patterns. Here, we use genomic-scale restriction-site-associated DNA sequencing (RADseq) data to test for distinct lineages in the endangered Eastern Massasauga Rattlesnake (Sistrurus catenatus). We then use coalescent-based modeling techniques to identify the evolutionary mechanisms responsible for the origin of the lineages in this species. We find equivocal evidence for distinct phylogenetic lineages within S. catenatus east of the Mississippi River, but strong support for a previously unrecognized lineage on the western edge of the range of this snake, represented by populations from Iowa, USA. Snakes from these populations show patterns of genetic admixture with a nearby non-threatened sister species (Sistrurus tergeminus). Tests of historical demographic models support the hypothesis that the genetic distinctiveness of Iowa snakes is due to a combination of isolation and historical introgression between S. catenatus and S. tergeminus. Our work provides an example of how model-based analysis of genomic-scale data can help identify conservation units in rare species.
OrthoFinder output from rice infecting Magnaporthe oryzae isolates
<p>Generated using OrthoFinder 2 and proteomes from 162 rice infecting Magnaporthe oryzae isolates</p>
Data and code for Resolving the Taxonomic Status of the Marbled Toad (Bufonidae: Incilius marmoreus): 2RAD-based Phylogeography Including an Isolated Population in Veracruz, Mexico
<p>Data and code for a study of the phylogeography of the Marbled Toad (<em>Incilius marmoreus</em>).</p>
JU394 C. elegans Wild Isolate (Hermanville, France) | 2011-06-30T10:41:40+01:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=JixqDi1_vBI</li> <li><b>strain</b> : JU394</li> <li><b>timestamp</b> : 2011-06-30T10:41:40+01:00</li> <li><b>gene</b> : -N/A-</li> <li><b>chromosome</b> : -N/A-</li> <li><b>allele</b> : -N/A-</li> <li><b>strain_description</b> : C. elegans Wild Isolate (Hermanville, France)</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : clockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : 655 JU394 on food L_2011_06_30__10_41_40___1___3</li> <li><b>total time (s)</b> : 899.0</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.40989</li> <li><b>number of segmented skeletons</b> : 26748</li> </ul>
JU394 C. elegans Wild Isolate (Hermanville, France) | 2011-06-14T14:44:20+01:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=_X2JcYLXx2c</li> <li><b>strain</b> : JU394</li> <li><b>timestamp</b> : 2011-06-14T14:44:20+01:00</li> <li><b>gene</b> : -N/A-</li> <li><b>chromosome</b> : -N/A-</li> <li><b>allele</b> : -N/A-</li> <li><b>strain_description</b> : C. elegans Wild Isolate (Hermanville, France)</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : anticlockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : 655 JU394 on food R_2011_06_14__14_44_20__9</li> <li><b>total time (s)</b> : 899.0</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.45319</li> <li><b>number of segmented skeletons</b> : 26595</li> </ul>
ED3017 C. elegans Wild Isolate (Ediburgh, Scotland) | 2011-06-14T15:16:43+01:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=Vrt5xChgaHQ</li> <li><b>strain</b> : ED3017</li> <li><b>timestamp</b> : 2011-06-14T15:16:43+01:00</li> <li><b>gene</b> : -N/A-</li> <li><b>chromosome</b> : -N/A-</li> <li><b>allele</b> : -N/A-</li> <li><b>strain_description</b> : C. elegans Wild Isolate (Ediburgh, Scotland)</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : anticlockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : 422 JU3017 on food R_2011_06_14__15_16_43__11</li> <li><b>total time (s)</b> : 72.594</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.45319</li> <li><b>number of segmented skeletons</b> : 2181</li> </ul>
JU394 C. elegans Wild Isolate (Hermanville, France) | 2011-06-30T10:43:00+01:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=JK1dUA6gyhg</li> <li><b>strain</b> : JU394</li> <li><b>timestamp</b> : 2011-06-30T10:43:00+01:00</li> <li><b>gene</b> : -N/A-</li> <li><b>chromosome</b> : -N/A-</li> <li><b>allele</b> : -N/A-</li> <li><b>strain_description</b> : C. elegans Wild Isolate (Hermanville, France)</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : clockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : 655 JU394 on food L_2011_06_30__10_43___3___3</li> <li><b>total time (s)</b> : 898.967</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.91191</li> <li><b>number of segmented skeletons</b> : 26635</li> </ul>
PS312 P. pacificus Wild Isolate | 2011-06-15T15:34:35+01:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=rxoLSwg3QgE</li> <li><b>strain</b> : PS312</li> <li><b>timestamp</b> : 2011-06-15T15:34:35+01:00</li> <li><b>gene</b> : -N/A-</li> <li><b>chromosome</b> : -N/A-</li> <li><b>allele</b> : -N/A-</li> <li><b>strain_description</b> : P. pacificus Wild Isolate</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : anticlockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : N2-L2</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Monika Leubner</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : 197 PS312 8 on N2-LL2 R_2011_06_15__15_34_35___8___3</li> <li><b>total time (s)</b> : 7191.73</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.52754</li> <li><b>number of segmented skeletons</b> : 83450</li> </ul>
PS312 P. pacificus Wild Isolate | 2011-06-22T10:35:30+01:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=Rxc1I8qml3U</li> <li><b>strain</b> : PS312</li> <li><b>timestamp</b> : 2011-06-22T10:35:30+01:00</li> <li><b>gene</b> : -N/A-</li> <li><b>chromosome</b> : -N/A-</li> <li><b>allele</b> : -N/A-</li> <li><b>strain_description</b> : P. pacificus Wild Isolate</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : anticlockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : no food</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Monika Leubner</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : 197 PS312 8 off food R_2011_06_22__10_35_30___8___1</li> <li><b>total time (s)</b> : 7183.66</li> <li><b>frames per second</b> : 27.248</li> <li><b>video micrometers per pixel</b> : 4.52754</li> <li><b>number of segmented skeletons</b> : 158418</li> </ul>
PS312 P. pacificus Wild Isolate | 2011-07-01T11:19:22+01:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=cWJoqJo3fmw</li> <li><b>strain</b> : PS312</li> <li><b>timestamp</b> : 2011-07-01T11:19:22+01:00</li> <li><b>gene</b> : -N/A-</li> <li><b>chromosome</b> : -N/A-</li> <li><b>allele</b> : -N/A-</li> <li><b>strain_description</b> : P. pacificus Wild Isolate</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : clockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : PS312-J3</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Monika Leubner</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : 197 PS312 8 on PS312-J3 L_2011_07_01__11_19_22___8___1</li> <li><b>total time (s)</b> : 4468.86</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.52367</li> <li><b>number of segmented skeletons</b> : 131636</li> </ul>
PS312 P. pacificus Wild Isolate | 2011-05-13T11:16:56+01:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=-F7PmO8pI0k</li> <li><b>strain</b> : PS312</li> <li><b>timestamp</b> : 2011-05-13T11:16:56+01:00</li> <li><b>gene</b> : -N/A-</li> <li><b>chromosome</b> : -N/A-</li> <li><b>allele</b> : -N/A-</li> <li><b>strain_description</b> : P. pacificus Wild Isolate</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : clockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Monika Leubner</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : 197 PS312 1 on food L_2011_05_13__11_16_56___8___1</li> <li><b>total time (s)</b> : 898.967</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.52754</li> <li><b>number of segmented skeletons</b> : 26264</li> </ul>
ED3021 C. elegans Wild Isolate (Ediburgh, Scotland) | 2011-02-25T11:11:05+00:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=rm1IK5g04JY</li> <li><b>strain</b> : ED3021</li> <li><b>timestamp</b> : 2011-02-25T11:11:05+00:00</li> <li><b>gene</b> : -N/A-</li> <li><b>chromosome</b> : -N/A-</li> <li><b>allele</b> : -N/A-</li> <li><b>strain_description</b> : C. elegans Wild Isolate (Ediburgh, Scotland)</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : clockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : 343 ED3021 on food L_2011_02_25__11_11_05___8___2</li> <li><b>total time (s)</b> : 898.967</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.52754</li> <li><b>number of segmented skeletons</b> : 26918</li> </ul>
JU440 C. elegans Wild Isolate (Beauchne, France) | 2011-02-25T10:30:36+00:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=gvNj0vqG69o</li> <li><b>strain</b> : JU440</li> <li><b>timestamp</b> : 2011-02-25T10:30:36+00:00</li> <li><b>gene</b> : -N/A-</li> <li><b>chromosome</b> : -N/A-</li> <li><b>allele</b> : -N/A-</li> <li><b>strain_description</b> : C. elegans Wild Isolate (Beauchne, France)</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : clockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : 575 JU440 on food L_2011_02_25__10_30_36___8___1</li> <li><b>total time (s)</b> : 899.0</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.52754</li> <li><b>number of segmented skeletons</b> : 26702</li> </ul>
JU402 C. elegans Wild Isolate (Hermanville, France) | 2011-03-22T15:55:41+00:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=zoPKV9_UHKY</li> <li><b>strain</b> : JU402</li> <li><b>timestamp</b> : 2011-03-22T15:55:41+00:00</li> <li><b>gene</b> : -N/A-</li> <li><b>chromosome</b> : -N/A-</li> <li><b>allele</b> : -N/A-</li> <li><b>strain_description</b> : C. elegans Wild Isolate (Hermanville, France)</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : clockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : 371 JU402 on food L_2011_03_22__15_55_41___8___9</li> <li><b>total time (s)</b> : 898.967</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.52754</li> <li><b>number of segmented skeletons</b> : 26864</li> </ul>
PS312 P. pacificus Wild Isolate | 2011-06-20T11:00:32+01:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=29v0RHtncHk</li> <li><b>strain</b> : PS312</li> <li><b>timestamp</b> : 2011-06-20T11:00:32+01:00</li> <li><b>gene</b> : -N/A-</li> <li><b>chromosome</b> : -N/A-</li> <li><b>allele</b> : -N/A-</li> <li><b>strain_description</b> : P. pacificus Wild Isolate</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : anticlockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : no food</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Monika Leubner</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : 197 PS312 8 off food R_2011_06_20__11_00_32___8___1</li> <li><b>total time (s)</b> : 7191.73</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.52754</li> <li><b>number of segmented skeletons</b> : 179035</li> </ul>
RC301 C. elegans Wild Isolate (Freiburg, Germany) | 2011-03-15T12:05:07+00:00
<blockquote> <p>This experiment is part of the <em>C.elegans behavioural database</em>. For more information and the complete collection of experiments visit http://movement.openworm.org</p> </blockquote> <ul> <li><b>preview link</b> : https://www.youtube.com/watch?v=ak3b_8D0EWc</li> <li><b>strain</b> : RC301</li> <li><b>timestamp</b> : 2011-03-15T12:05:07+00:00</li> <li><b>gene</b> : -N/A-</li> <li><b>chromosome</b> : -N/A-</li> <li><b>allele</b> : -N/A-</li> <li><b>strain_description</b> : C. elegans Wild Isolate (Freiburg, Germany)</li> <li><b>sex</b> : hermaphrodite</li> <li><b>stage</b> : adult</li> <li><b>ventral_side</b> : clockwise</li> <li><b>media</b> : NGM agar low peptone</li> <li><b>arena</b> : <ul> <li><b>style</b> : petri</li> <li><b>size</b> : 35</li> <li><b>orientation</b> : away</li> </ul> </li> <li><b>food</b> : OP50</li> <li><b>habituation</b> : 30m wait</li> <li><b>who</b> : Laura Grundy</li> <li><b>protocol</b> : Method in E. Yemini et al. doi:10.1038/nmeth.2560. Worm transferred to arena 30 minutes before recording starts.</li> <li><b>lab</b> : <ul> <li><b>name</b> : William R Schafer</li> <li><b>location</b> : MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK</li> </ul> </li> <li><b>software</b> : <ul> <li><b>name</b> : tierpsy (https://github.com/ver228/tierpsy-tracker)</li> <li><b>version</b> : cbfc23eb4f1ac2f29be75ade7a937eed58a5b219</li> <li><b>featureID</b> : @OMG</li> </ul> </li> <li><b>base_name</b> : 883 RC301 on food L_2011_03_15__12_05_07___8___3</li> <li><b>total time (s)</b> : 898.967</li> <li><b>frames per second</b> : 30.03</li> <li><b>video micrometers per pixel</b> : 4.52754</li> <li><b>number of segmented skeletons</b> : 26726</li> </ul>
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.