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Microbiome profiling of feces from mice flown on the RR-10 mission

The objective of the Rodent Research-10 Mission (RR-10) was to investigate how spaceflight affects the cellular and molecular mechanisms of normal bone tissue regeneration in space. To this end, ten (10) 14-15 weeks-old female B6129SF2/J Wild Type (WT), and ten (10) 14-15 weeks-old female B6;129S2-Cdkn1atm1Tyj/J (p21-null) mice received a pre-flight subcutaneous injection of the bone marker (Alizarin Red), and were then delivered to the ISS aboard SpaceX-21. At 7 days before euthanasia, all 20 mice received an intraperitoneal (IP) injection with a bone formation marker (Calcein). At 48 +/- 2 hours before euthanasia, all 20 mice received an IP injection with a second dose of Calcein as well as a cell proliferation marker (BrdU). Then, following 28-29 days in microgravity, the Flight mice were euthanized. Following removal of hindlimbs, carcasses were wrapped in aluminum foil, preserved in the CryoChiller, and stored at -80 C or colder until return to Earth. In addition to the Flight group, three ground control groups were also part of the study: Basal (representing the pre-launch state), Vivarium (standard vivarium housing for the same duration of time as flight), and Ground (flight habitat in the International Space Station Environment Simulator, ISSES). Twenty mice (10 of each strain) were included in each of these control groups (except Vivarium which included 12 of each strain). These were treated, euthanized and processed on the same schedule and in the same manner as the flight samples. At the end of RR-10 experiment, all frozen carcasses were partially thawed and fecal pellets were removed and preserved by flash freezing in LN2. DNA was extracted, shotgun metagenome libraries generated, and libraries sequenced (target 10 M clusters at PE 250 bp). This dataset features WT samples from the Flight (n=3), Ground (n=5), Basal (n=10) and Vivarium groups (n=12), as well as p21-null samples from Flight (n=5), Ground (n=5), Basal (n=10) and Vivarium groups (n=12).

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3340 V1.0

This is a Mars Express Radio Science data set, collected during the extended mission phase 2010-01-01 to 2012-12-31. It is a Global Gravity measurement and covers the time 2012-07-07T06:43:24.000 to 2012-07-07T10:30:04.500.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3326 V1.0

Bulk RNA sequencing and single nuclei transcriptomics and epigenomics of brain tissue from mice flown on the RR-10 mission

The objective of the Rodent Research-10 mission (RR-10) was to investigate how spaceflight affects the cellular and molecular mechanisms of normal bone tissue regeneration in space. To this end, ten (10) 14-15 weeks-old female B6129SF2/J Wild Type (WT), and ten (10) 14-15 weeks-old female B6;129S2-Cdkn1atm1Tyj/J (p21-null) mice received a pre-flight subcutaneous injection of the bone marker (Alizarin Red), and were then delivered to the ISS aboard SpaceX-21. At 7 days before euthanasia, all 20 mice received an intraperitoneal (IP) injection with a bone formation marker (Calcein). At 48 +/- 2 hours before euthanasia, all 20 mice received an IP injection with a second dose of Calcein as well as a cell proliferation marker (BrdU). Then, following 28-29 days in microgravity, the Flight mice were euthanized. Following removal of hindlimbs, carcasses were wrapped in aluminum foil, preserved in the CryoChiller, and stored at -80 C or colder until return to Earth. In addition to the Flight group, three ground control groups were also part of the study: Basal (representing the pre-launch state), Vivarium (standard vivarium housing for the same duration of time as flight), and Ground (flight habitat in the International Space Station Environment Simulator, ISSES). Twenty mice (10 of each strain) were included in each of these control groups (except Vivarium which included 12 of each strain). These were treated, euthanized and processed on the same schedule and in the same manner as the flight samples. This study includes bulk RNA sequencing data from left cerebral hemispheres from 4 WT flight animals and 5 WT ground control animals, and single nuclei transcriptomics and epigenomics data from left cerebral hemispheres from 5 WT flight animals, and 5 WT ground control animals.

Transcriptional profiling, protein expression profiling, and phosphoprotein profiling of kidneys from mice flown on the RR-10 mission

The objective of the Rodent Research-10 Mission (RR-10) was to investigate how spaceflight affects the cellular and molecular mechanisms of normal bone tissue regeneration in space. To this end, ten (10) 14-15 weeks-old female B6129SF2/J Wild Type (WT), and ten (10) 14-15 weeks-old female B6;129S2-Cdkn1atm1Tyj/J (p21-null) mice received a pre-flight subcutaneous injection of the bone marker (Alizarin Red), and were then delivered to the ISS aboard SpaceX-21. At 7 days before euthanasia, all 20 mice received an intraperitoneal (IP) injection with a bone formation marker (Calcein). At 48 +/- 2 hours before euthanasia, all 20 mice received an IP injection with a second dose of Calcein as well as a cell proliferation marker (BrdU). Then, following 28-29 days in microgravity, the Flight mice were euthanized. Following removal of hindlimbs, carcasses were wrapped in aluminum foil, preserved in the CryoChiller, and stored at -80 C or colder until return to Earth. In addition to the Flight group, three ground control groups were also part of the study: Basal (representing the pre-launch state), Vivarium (standard vivarium housing for the same duration of time as flight), and Ground (flight habitat in the International Space Station Environment Simulator, ISSES). Twenty mice (10 of each strain) were included in each of these control groups (except Vivarium which included 12 of each strain). These were treated, euthanized and processed on the same schedule and in the same manner as the flight samples. At the end of RR-10 experiment, all frozen carcasses were partially thawed and kidney tissues were removed and preserved by flash freezing in LN2. Kidneys were kept at -80 C freezer until processing. To ensure that both transcriptional profiling and protein expression profiling datasets are representative of the entire kidney, whole kidneys were first pulverized into a fine powder on dry ice. This powder was then split into two equal fractions with half being used for RNA isolation and half being used for protein isolation. RNA was used to generate three different transcriptional profiling datasets: a 3' tag-Seq datasets (20 M clusters at SE 93 bp), a polyA enriched dataset (60 M clusters at PE 150 bp), and a ribodepleted dataset (60 M clusters at PE 150 bp). Protein was used to generate protein expression profiling, and phosphoprotein profiling using the iTRAQ method (Isobaric tags for relative and absolute quantitation). Transcriptional profiling dataset features WT samples from the Flight, Ground, Basal and Vivarium groups. Protein expression profiling and phosphoprotein profiling datasets exclude the Vivarium group.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3363 V1.0

Bulk RNA sequencing and single nuclei transcriptomics and epigenomics of brain tissue from mice flown on the RRRM-2 mission

In the Rodent Research Reference Mission (RRRM-2), forty female C57BL/6NTac mice were flown on the International Space Station. To assess differences in outcomes due to age, twenty 12 week-old and twenty 29 week-old mice were flown, respectively. To directly assess spaceflight effects, half of the young and old mice (10 old, 10 young) were sacrificed on-orbit after 55-58 days (ISS Terminal, ISS-T), while the other half (10 old, 10 young) were returned live to Earth after 32 days and allowed to recover for 24 days (Live Animal Return, LAR) before sacrifice. ISS-T and LAR mice were the same age at sacrifice. Both the ISS-T and LAR animals had independent ground controls (10 mice per group housed in flight hardware in matched environmental conditions), basal controls (10 mice per group sacrificed 2 days before launch), and vivarium controls (10 mice per group housed within standard vivarium habitats). Thus RRRM-2 included a total of 160 mice. This study includes bulk RNA sequencing and single nuclei transcriptomics and epigenomics from left cerebral hemispheres from 4 young ISS-T spaceflight animals, 5 old ISS-T spaceflight animals, 5 young ISS-T ground control animals, and 4 young ISS-T ground control animals.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3355 V1.0

MARS EXPRESS SUN MRS 1/2/3 EXTENDED MISSION 3 2755 V1.0

This is a Mars Express Radio Science data set, collected during the extended mission phase 2010-01-01 to 2012-12-31. It is a Solar Conjunction measurement and covers the time 2011-02-03T09:00:39 to 2011-02-03T10:59:56.500.

Hayabusa2 Mission Bundle

This bundle collects all the mission wide information needed to use and understand the scientific data products produced by the Hayabusa2 mission.

Transcriptional profiling of mammary glands from mice flown on the RRRM-1 mission

In Rodent Research Reference Mission-1 (RRRM-1), also known as RR-8, forty female BALB/cAnNTac mice were flown on the International Space Station. To assess differences in outcomes due to age, twenty 10-12 week-old and twenty 32 week-old mice were flown, respectively. To directly assess spaceflight effects, half of the young and old mice (10 old, 10 young) were sacrificed on-orbit after 22-23 days (ISS Terminal, ISS-T), while the other half (10 old, 10 young) were returned live to Earth after 40 days and allowed to recover for 2 days (Live Animal Return, LAR) before sacrifice. Both the ISS-T and LAR animals had independent ground controls (10 mice housed in flight hardware in matched environmental conditions), basal controls (10 mice sacrificed 1 day after launch), and vivarium controls (10 mice housed within standard vivarium habitats). Thus RRRM-1 included a total of 160 mice. This datasets features ribodepleted total RNA-seq data from mammary glands dissected from six LAR groups (young and old for flight, ground control, and vivarium control). Data from 5-10 livers per group are included. All samples include either Mix 1 or Mix 2 of the ERCC spike-in control. Target depth was 60 M clusters sequenced at PE 150 bp.

Transcriptional profiling of spleens from mice flown on the RR-23 mission

The objective of the Rodent Research-23 missions (RR-23) was to better understand the effects of spaceflight on the eyes, specifically on the structure and function of the arteries, veins, and lymphatic vessels that are needed to maintain vision. To this end, twenty male, C57BL/6J, 16-17 weeks old mice were delivered to the ISS on SpaceX-21 in a single transporter, transferred to two rodent habitats, and maintained in microgravity for 38 days. Flight mice were then returned to Earth alive (Jan 13th, 2021). After splashdown in the Atlantic Ocean, mice were transported to Kennedy Space Center via helicopter. The 20 Flight, 20 Habitat Ground Control (HGC), and 20 Vivarium Ground Control (VGC) mice were removed from Rodent Transporters (Flight and HGC) or vivarium cages (VGC), placed into shipping containers, and flown to Texas A and M University. There, mice underwent post flight procedures, before euthanasia and tissue collection. Flight, HGC and VGC animals were euthanized and dissected on Jan 14th, 17th or 20th of 2021, respectively. Spleens were preserved by immersion in RNAlater and stored at -80C until RNA was extracted, and libraries generated and sequenced (target 60 M clusters per sample, PE 150 bp). This dataset features 9 samples from the Flight group, 10 samples from the HGC group, and 9 samples from the VGC group.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3282 V1.0

restrictedus-pdApr 2025View details →

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3371 V1.0

restrictednotspecifiedMar 2025View details →

Mission Reports IMPACTS V1

The Mission Reports IMPACTS dataset consists of flight plans, plans of the day, science plans, and science summaries logged by scientists during the Investigation of Microphysics and Precipitation for Atlantic Coast-Threatening Snowstorms (IMPACTS) field campaign. IMPACTS was a three-year sequence of winter season deployments conducted to study snowstorms over the U.S Atlantic Coast (2020-2022). The campaign aimed to (1) Provide observations critical to understanding the mechanisms of snowband formation, organization, and evolution; (2) Examine how the microphysical characteristics and likely growth mechanisms of snow particles vary across snowbands; and (3) Improve snowfall remote sensing interpretation and modeling to significantly advance prediction capabilities. The mission reports are available from January 17 through March 1, 2020 in PDF format. It should be noted that this dataset will be updated in subsequent years of the IMPACTS campaign.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3381 V1.0