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FIGURE 22 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 22. Male holotype of Boophis miadana sp. nov. (ZSM 5107/2005) from Andohahela, 1550 m a.s.l.: (A) dorsolateral view; (B) ventral view.

FIGURE 23 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 23. Male holotype of Boophis haingana sp. nov. (ZSM 5109/2005) from Andohahela: (A) dorsolateral view; (B) ventral view.

FIGURE 1. Maximum parsimony 16S in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 1. Maximum parsimony 16S rRNA phylogram for species in the Boophis majori and B. microtympanum groups. From 480 total characters, 336 were constant and 129 parsimony informative. MP searches retained 39 trees of which a strict consensus is shown. Consensus support values higher than 50, from 2000 bootstrap replicates, are shown; an asterisk indicates Bayesian posterior probabilities equal or higher than 95%. Species newly described or resurrected herein are in bold.

FIGURE 18. Maximum parsimony 16S in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 18. Maximum parsimony 16S rRNA phylogram for species in the Boophis luteus group. From 470 total characters, 347 were constant and 111 parsimony informative. MP searches retained 60 trees of which a strict consensus is shown. Consensus support values higher than 50, from 2000 bootstrap replicates, are shown; an asterisk indicates Bayesian posterior probabilities equal or higher than 95%. The species newly described herein is in bold.

FIGURE 12 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 12. Male holotype of Boophis roseipalmatus sp. nov. (ZSM 211/2004) from Montagne d'Ambre National Park: (A) dorsolateral view; (B) ventral view.

FIGURE 3 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 3. Spectrograms and waveforms of calls of Boophis piperatus sp. nov. from near Vohiparara, Ranomafana National Park (recorded on 28 January 2004, air temperature 20–21°C): (A) call type 1, (B) call type 2 (see text).

FIGURE 16. Maximum parsimony 16S in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 16. Maximum parsimony 16S rRNA phylogram for species in the Boophis albilabris group. From 535 total characters, 445 were constant and 35 parsimony informative. MP searches retained 6 trees of which a strict consensus is shown. Consensus support values higher than 50, from 2000 bootstrap replicates, are shown; an asterisk indicates Bayesian posterior probabilities equal or higher than 95%. The species newly described herein is in bold.

FIGURE 4 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 4. Dorsolateral view (A) and ventral view (B) of male of Boophis andrangoloaka (FGZC 2139) from Ambohitantely; (C) dorsolateral view of Boophis andrangoloaka (ZFMK 60134) from Ambohitantely; dorsolateral view (D) and ventral view (E) of a dark coloured specimen of Boophis rhodoscelis from Ranomafanakely; (F) dorsolateral view of a light coloured specimen of Boophis rhodoscelis from Ranomafanakely.

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FIGURE 3 in High haplotype diversity in a microendemic Malagasy gecko species, Lygodactylus mirabilis (Pasteur, 1962)

FIGURE 3: Haplotype network of L. mirabilis using all the 31 sampled individuals (1251 bp, cytochrome b and 16S rRNA genes). Circle size indicates the frequency of the haplotype, as indicated by the circles on the left side of the figure. Black dots indicate missing haplotypes. Straight lines between two haplotypes indicate that they differ by one mutation.

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FIGURE 1. A in High haplotype diversity in a microendemic Malagasy gecko species, Lygodactylus mirabilis (Pasteur, 1962)

FIGURE 1. A) Map of Madagascar with indicated the location of the Ankaratra Massif. B) Distribution map of the surveyed area along the mountain peaks of the Ankaratra Massif in Madagascar (see Material and Methods for further explanations). White points indicate where Lygodactylus mirabilis specimens have been found; grey points indicate the locations of the individuals sampled and used for the genetic analysis. C) Altitudinal range of the recorded individuals of L. mirabilis found. Grey areas are proportional to the number of L. mirabilis eggs found at specific altitudes.

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FIGURE 1 in Molecular phylogeography of a widespread Malagasy leaf chameleon species, Brookesia superciliaris

FIGURE 1. Phylogenetic relationships of Brookesia superciliaris inferred from the Bayesian and maximum Likelihood analyses of 12S rRNA gene sequence data. Numbers above the branches represent posterior probability values. Numbers below the branches represent maximum likelihood bootstrap values. Sequences of Brookesia therezieni were used as outgroup. Inset picture shows a specimen of B. superciliaris. Map shows sampling localities.

Figure 5 in An integrative approach to characterize Malagasy bats of the subfamily Vespertilioninae Gray, 1821, with the description of a new species of Hypsugo

Figure 5. Echolocation calls of the holotype of Hypsugo bemainty sp. nov. (FMNH 217884), Neoromicia malagasyensis (FMNH 222724), Neoromicia matroka (FMNH 222727), Neoromicia robertsi (FMNH 222729), Pipistrellus hesperidus (FMNH 209270), and Pipistrellus raceyi (FMNH 217886).

Figure 9 in An integrative approach to characterize Malagasy bats of the subfamily Vespertilioninae Gray, 1821, with the description of a new species of Hypsugo

Figure 9. Different views of skull and mandible of Hypsugo bemainty sp. nov. (FMNH 217884, SMG 17029), holotype from Province de Toliara, Kirindy (CNFEREF). Pictures include dorsal view of cranium (upper row, left), ventral view of cranium (upper row, right), and lateral view of cranium and mandible (lower row). (Photograph taken by J. Weinstein, Field Museum image number Z95227_d.)

Figure 4 in An integrative approach to characterize Malagasy bats of the subfamily Vespertilioninae Gray, 1821, with the description of a new species of Hypsugo

Figure 4. Principal Component Analysis plots of 11 craniodental measurements for genotyped Malagasy vesper speci- mens for A, PC1 vs. PC2, and B, PC1 vs. PC3. Information on component loadings is presented in Table 5.

Figure 1 in An integrative approach to characterize Malagasy bats of the subfamily Vespertilioninae Gray, 1821, with the description of a new species of Hypsugo

Figure 1. Map of localities mentioned in text associated with sequenced members of the genera Neoromicia, Pipistrellus, and Hypsugo captured on Madagascar. Other localities mentioned in the text and an elevational gradient are also shown.

Figure 8 in An integrative approach to characterize Malagasy bats of the subfamily Vespertilioninae Gray, 1821, with the description of a new species of Hypsugo

Figure 8. Dorsal (above) and lateral (below) views of bacula from two species of Pipistrellus. A, Pipistrellus raceyi (FMNH 217886, total length 8.90 mm) from Kirindy Forest (CNFEREF); B, Pipistrellus raceyi (FMNH 222722, total length 6.10 mm) from near Andasibe; C, Pipistrellus hesperidus (FMNH 176094, average total length of four specimens 1.9 mm) from Parc National de Kirindy Mitea (redrawn from Bates et al., 2006, Fig. 10A); D, Pipistrellus hesperidus (FMNH 209270, total length 1.90 mm) from Grotte de Sarodrano; E, Pipistrellus hesperidus (FMNH 217905, total length 1.70 mm) from Grotte de Sarodrano; F: Pipistrellus hesperidus (TM 48624, UP-840, total length 2.18 mm) from South Africa.

Figure 10 in An integrative approach to characterize Malagasy bats of the subfamily Vespertilioninae Gray, 1821, with the description of a new species of Hypsugo

Figure 10. Dorsal (above) and lateral (below) views of bacula from two species of Hypsugo. A, Hypsugo bemainty sp. nov. (FMNH 217884, holotype, 1.79 mm) from Kirindy (CNFEREF); B, Hypsugo bemainty (FMNH 213577, total length 1.50 mm) from Kirindy (CNFEREF); C, Hypsugo anchietae (TM 48489, ECJS-120/2009, total length 1.25 mm) from Botswana.

Figure 3. Majority rule 50 in An integrative approach to characterize Malagasy bats of the subfamily Vespertilioninae Gray, 1821, with the description of a new species of Hypsugo

Figure 3. Majority rule 50% consensus neighbour-joining (NJ) proportional cladogram generated from partial cytochrome b sequences (1201 bp). The NJ analysis was conducted using Kimura 2-Parameter (K2P) distance and implemented in PAUP* 4.10b for Unix (Swofford, 2003), incorporating 1000 bootstrap pseudoreplicates. Numbers at nodes indicate bootstrap support. The geographical origins of the different samples per species are indicated as A = Africa and M = Madagascar. Specimens are designated by their voucher number or, where they have not yet been catalogued, by their field number (see Table S1 for definitions of different acronyms).

Figure 2 in An integrative approach to characterize Malagasy bats of the subfamily Vespertilioninae Gray, 1821, with the description of a new species of Hypsugo

Figure 2. Consensus Bayesian tree generated from partial sequences of cytochrome b (680 bp), applying the model GTR + I + G and implemented in MrBayes 3.2 (Huelsenbeck & Ronquist, 2001; Ronquist et al., 2012). The first number at each node indicates posterior probability and the second indicates bootstrap support as generated by RAxML (Stamatakis, 2006; Stamatakis et al., 2008; Stamatakis & Ott, 2008), employing the model GTR+G. An asterisk (*) indicates that support for that node is greater than 0.95 posterior probabilities in the Bayesian analysis and 95% bootstrap support in the ML analysis. The clade identity of every sequenced specimen is provided in Figure 3. The geographical origins of the different samples per species are indicated as A = Africa and M = Madagascar. Numbers in parentheses indicate the number of sequences included in the analysis for that clade as per the reduced dataset. See Monadjem et al. (2010) for the definition of Neoromicia cf. melckorum. The clade identity of every sequenced individual is provided in Figure 3.

Figure 7 in An integrative approach to characterize Malagasy bats of the subfamily Vespertilioninae Gray, 1821, with the description of a new species of Hypsugo

Figure 7. Dorsal (above) and lateral (below) views of bacula from six species of Neoromicia. A, Neoromicia matroka (FMNH 222725, total length 2.12 mm) from Mantadia; B, Neoromicia matroka (FMNH 222728, total length 2.26 mm) from Anjozorobe; C, Neoromicia malagasyensis (FMNH 222724, total length 2.25 mm) from Isalo; D, Neoromicia robertsi (FMNH 222729, total length 3.12 mm), from Anjozorobe; E, Neoromicia capensis, dorsal view (TM 48490, ECJS-112/2009, total length 2.04 mm) and lateral view (TM 48485, ECJS-03/2009, lateral view, total length 2.01 mm) from Botswana; F: Neoromicia nana (TM 48573, UP-923, total length 1.32 mm), from South Africa; G, Neoromicia cf. melckorum (NMZL ECJS-114/ 2010, total length 2.55 mm) from Zambia.